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Summary of Contents for VWR ShiroGel 700-0250
- Page 1 VWR ShiroGel Horizontal Electrophoresis Gel Boxes INSTRUCTION MANUAL European Catalogue Number(s): 7x10cm 700-0250 15x10cm 700-0253 15x15cm 700-0255 Version: Issued: 08.04.2010 VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010...
- Page 2 Legal Address of Manufacturer United States Europe VWR International VWR International bvba 1310 Goshen Parkway Researchpark Haasrode 2020 West Chester, PA 19380 Geldenaaksebaan 464 800-932-5000 B-3001 Leuven http://www.vwr.com + 32 16 385011 http://be.vwr.com Country of origin VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010...
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Page 3: Table Of Contents
Table of Contents Page Warning Safety information Package Contents Unpacking Installation Intended Use Symbols and conventions Description of Buttons and Switches Instructions for use Troubleshooting General maintenance Technical Service Warranty Disposal Appendix Accessories VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... -
Page 4: Warning
Rinsed with 0.1% DEPC- (diethyl pyrocarbonate) treated distilled water, Caution: DEPC is a suspected carcinogen. Always wear gloves and safety glasses. RNaseZAP™ (Ambion) can also be used. Please consult the instructions for use with acrylic gel tanks. VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... -
Page 5: Package Contents
These product descriptions and included component information should be referred to as soon as the units are received to ensure that all components have been included. The unit should be checked for damage when received. Please contact your supplier if there are any problems or missing items. VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... -
Page 6: Installation
2. Remove the tray. 3. Peel the back off of the loading guide and carefully apply the loading guide directly to the gel box. The unit is now ready for use. VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... -
Page 7: Intended Use
CAUTION This symbol indicates the presence of high voltage and warns the user to proceed with caution Description of Buttons and Switches Electrode wires Replaceable electrodes Height adjustable combs Rubber casting dams VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... -
Page 8: Instructions For Use
The gel must be cooled to between 50°C and 60°C degrees before pouring. Gel Pouring:- The VWR systems allow two different methods of gel casting:- Casting Dams and Traditional Tape Using trays with Casting Dams 1. - Page 9 Note: DNA, RNA have a net negative charge and will migrate towards the anode. 3. Load the samples into the wells using pipettes. Multi-channel pipettes can be used for loading samples with MC compatible combs, see listing in accessories for identification of these. VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010...
- Page 10 However if the gel bands are too faint then the staining procedure should be adjusted so that there is less de-staining. If there is too much background then the staining procedure should be adjusted so that there is more de-staining. VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010...
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Page 11: Troubleshooting
The units should only be cleaned with the following:- Warm water with a low concentration of soap or other mild detergent. Compatible detergents include dishwashing liquid. The units should not be left in detergents for more than 30 minutes. VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... -
Page 12: Technical Service
(2) years from date of purchase. If a defect is present, VWR will, at its option, repair, replace, or refund the purchase price of this product at no charge to you, provided it is returned during the warranty period. -
Page 13: Disposal
By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health. Thank you VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... -
Page 14: Appendix
0.25% xylene cyanole FF in 1x TAE buffer. Only 1–10 ml of the 10x loading dye should be prepared. Ethidium Bromide Solution 10mg/ml Add 10 mg of Ethidium Bromide to 1 ml distilled water. VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... -
Page 15: Accessories
Comb MC 30 sample wells 1,5mm for 15cm gel tray shiroGEL 700-0287 Comb 35 sample wells 1,5mm for 15cm gel tray shiroGEL 700-0288 Comb 8 sample wells 1,0mm for 15cm gel tray shiroGEL 700-0289 Comb 8 sample wells 1,5mm for 15cm gel tray shiroGEL VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010... - Page 16 Local VWR offices in Europe and Asia Pacific Austria Hungary Portugal VWR International GmbH Spektrum-3D Ltd. VWR International - Material de Graumanngasse 7 A VWR International Company Laboratório, Lda 1150 Wien Simon László u. 4. Edifício Neopark Tel.: 01 97 002 0 4034 Debrecen Av.