Agilent Technologies InfinityLab 1290 Infinity II User Manual
  1. Manuals
  2. Brands
  3. Agilent Technologies Manuals
  4. Security Sensors
  5. InfinityLab 1290 Infinity II
  6. User manual
Agilent Technologies InfinityLab 1290 Infinity II User Manual

Agilent Technologies InfinityLab 1290 Infinity II User Manual

Evaporative light scattering detectors
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
Agilent InfinityLab
Evaporative Light Scattering Detectors
User Manual
Agilent InfinityLab ELSD User Manual

Advertisement

Table of Contents
loading

Related Manuals for Agilent Technologies InfinityLab 1290 Infinity II

Summary of Contents for Agilent Technologies InfinityLab 1290 Infinity II

  • Page 1 Agilent InfinityLab Evaporative Light Scattering Detectors User Manual Agilent InfinityLab ELSD User Manual...
  • Page 2 CAUTION beyond a notice until ble for errors or for incidental or conse- sent from Agilent Technologies, Inc. as the indicated conditions are fully governed by United States and interna- quential damages in connection with the understood and met.
  • Page 3 In This Book This manual contains information on: • Agilent 1260 Infinity II ELSD (G4260B) • Agilent 1290 Infinity II ELSD (G7102A) Introduction to the Detector This chapter gives an introduction to the module, instrument overview, and internal connectors. Site Requirements and Specifications This chapter provides information on environmental requirements, physical, and performance specifications.
  • Page 4 Maintenance This chapter describes the maintenance of the ELSD. Parts and Materials for Maintenance This chapter provides information on parts for maintenance. Appendix This chapter provides addition information on safety, legal, and Web. Agilent InfinityLab ELSD User Manual...

  • Page 5: Table Of Contents

    Contents Introduction to the Detector Introduction to the ELSD System Overview Site Requirements and Specifications Pre-installation Requirements Site Requirements Physical Specifications Performance Specifications LAN Configuration What You Have to Do First TCP/IP Parameter Configuration PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Software Setup Using the Module Before Using the Detector...
  • Page 6 Cleaning Evaporator Tube Putting the Instrument into Storage Updating Detector Firmware Parts and Materials for Maintenance Identifying Parts and Materials Appendix General Safety Information Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Agilent Technologies on Internet Agilent InfinityLab ELSD User Manual...

  • Page 7: Introduction To The Detector

    Introduction to the Detector Introduction to the ELSD System Overview Basic Principles of Operation Operational Parameters Overview of ELS Detector This chapter gives an introduction to the module, instrument overview, and internal connectors. Agilent InfinityLab ELSD User Manual...

  • Page 8: Introduction To The Elsd

    Introduction to the Detector Introduction to the ELSD Introduction to the ELSD The Evaporative Light Scattering Detector is a unique and highly sensitive detector for semi-volatile and non-volatile solutes in a liquid stream. It is mainly used as a concentration detector for High Performance Liquid Chromatography (HPLC).

  • Page 9: System Overview

    Introduction to the Detector System Overview System Overview Basic Principles of Operation Nebulization The eluent inlet is connected to the nebulizer via a short length of stainless steel capillary tube. The incoming eluent stream passes through the heated nebulizer and is mixed with the incoming nebulizer gas stream. The mixed gas and eluent stream form an aerosol plume containing a uniform dispersion of droplets that then passes as a continuous flow into the evaporator section.
  • Page 10 Introduction to the Detector System Overview Detection Light in the optical chamber is passed through the instrument at right angles to the direction of particle flow. A light trap is located opposite the source of light to capture the transmitted incident beam eliminating internal reflections within the instrument body.
  • Page 11 Introduction to the Detector System Overview Theory There are four main processes, by which the path of electromagnetic radiation or light can change direction, when passing through a medium containing a suspended particulate phase, see Figure 2 on page 11. Reflection Refraction Rayleigh...

  • Page 12: Operational Parameters

    Introduction to the Detector System Overview Operational Parameters The ELS Detector responds to all compounds that are less volatile than the mobile phase and is independent of a compound’s optical properties. It therefore provides advantages over other spectroscopic detectors for detecting compounds that are deficient in a UV chromophore or fluorophore.

  • Page 13: Overview Of Els Detector

    Introduction to the Detector System Overview Overview of ELS Detector Figure 3 ELSD overview (front) Front screen display Keypad Eluent inlet Solvent waste outlet Agilent InfinityLab ELSD User Manual...
  • Page 14 Introduction to the Detector System Overview Figure 4 ELSD overview (rear) Vapor Sensor vent Serial RS232 port LAN connector (only active on G7102A) Firmware button I/O connector (Remote Start input) Mains Switch Mains Input Gas Inlet port Exhaust port Agilent InfinityLab ELSD User Manual...
  • Page 15 1, 5, 6, 11 To make appropriate remote start and A/Z connections from a third-party LC, a NOTE third-party remote start cable for Dimension software (Remote start cable (PL0890-0350)) can be purchased from Agilent Technologies. Agilent InfinityLab ELSD User Manual...

  • Page 16: Site Requirements And Specifications

    Site Requirements and Specifications Pre-installation Requirements Site Requirements Physical Specifications Physical Specifications Agilent 1260 Infinity II ELSD (G4260B) Physical Specifications Agilent 1290 Infinity II ELSD (G7102A) Performance Specifications Performance Specifications (G4260B) Performance Specifications (G7102A) This chapter provides information on environmental requirements, physical, and performance specifications.

  • Page 17: Pre-Installation Requirements

    Site Requirements and Specifications Pre-installation Requirements Pre-installation Requirements For a detailed description of the environmental and operating requirements of the ELSD, see the Site Preparation Checklist. This manual will give you an overview of the ELS detector and describe its operation in more detail.

  • Page 18: Site Requirements

    Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument, see the Site Preparation Checklist for more details. Laser Safety The Agilent 1290 Infinity II ELSD (G7102A) is classified as a "Laser Class 1" product (IEC825-1, CFR1040.10 &...
  • Page 19 Never operate your instrumentation from a power outlet that has no  ground connection. Never use a power cord other than the Agilent Technologies power cord  designed for your region. Use of unsupplied cables...
  • Page 20 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 2 on page 21) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 15 cm (5.9 inches) in the rear for air circulation and electric connections.

  • Page 21: Physical Specifications

    Site Requirements and Specifications Physical Specifications Physical Specifications Physical Specifications Agilent 1260 Infinity II ELSD (G4260B) Table 2 Physical Specifications G4260B Type Specification Comments Weight 11.0 kg (24.3 lbs non-cooled) Dimensions 420 x 200 x 450 mm (height × width × depth) (16.5 x 7.9 x 17.7 inches) Line voltage 100 –...

  • Page 22: Physical Specifications Agilent 1290 Infinity Ii Elsd (G7102A)

    Site Requirements and Specifications Physical Specifications Physical Specifications Agilent 1290 Infinity II ELSD (G7102A) Table 3 Physical Specifications G7102A Type Specification Comments Weight 11.0 kg (24.3 lbs, non-cooled), 13.3 kg (29.3 lbs, cooled) Dimensions 420 x 200 x 450 mm (height ×...

  • Page 23: Performance Specifications

    Site Requirements and Specifications Performance Specifications Performance Specifications Performance Specifications (G4260B) Table 4 Performance Specifications G4260B Type Specification Comment Light Source LED 480 nm (Class 1 LED product) Detector PMT with digital signal processing Nebulizer OFF, 25 – 90 °C Evaporator Non-cooled OFF, 25 –...
  • Page 24 Site Requirements and Specifications Performance Specifications Table 4 Performance Specifications G4260B Type Specification Comment PC control ELSD driver for OpenLab ChemStation edition ELSD driver for OpenLab EZChrom edition LC and CE Drivers Rev. A.02.11 or above Safety and maintenance Gas shut-off Valve, Leak Detection, Laser Interlock In order to control OpenLab CDS edition, the ELSD driver is required.

  • Page 25: Performance Specifications (G7102A)

    Site Requirements and Specifications Performance Specifications Performance Specifications (G7102A) Table 6 Performance Specifications G7102A Type Specification Comment Light Source LASER 405 nm, 10 mW (Class 3B) Detector Dual PMT with digital signal processing Nebulizer OFF, 25 – 90 °C Evaporator Non-cooled OFF, 25 –...
  • Page 26 Site Requirements and Specifications Performance Specifications Specification Conditions ASTM: “Evaporative Light Scattering Detectors Used in Liquid Chromatography”. Table 7 Reference conditions Gas flow 1.6 SLM Neb temperature 70 °C Evaporator temperature 70 °C Data Rate 40 Hz Smoothing ASTM drift tests require a temperature change below 2 °C/h (3.6 °F/h) over one hour period.

  • Page 27: Lan Configuration

    LAN Configuration What You Have to Do First TCP/IP Parameter Configuration Configuration Using Instrument Front Panel Configuration Using Web Browser With Telnet PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Software Setup PC Setup for Local Configuration Agilent ChemStation Setup This chapter provides information on connecting the module to the control software.

  • Page 28: What You Have To Do First

    LAN Configuration What You Have to Do First What You Have to Do First The ELSD has an on-board LAN communication interface. 1 Note the MAC (Media Access Control) address for further reference. The MAC or hardware address of the LAN interface is a worldwide unique identifier. No other network device will have the same hardware address.

  • Page 29: Tcp/Ip Parameter Configuration

    LAN Configuration TCP/IP Parameter Configuration TCP/IP Parameter Configuration To operate properly in a network environment, the LAN interface must be configured with valid TCP/IP network parameters. The parameters are: • IP address • Subnet Mask • Default Gateway The TCP/IP parameters can be configured by the following methods: •...

  • Page 30: Configuration Using Instrument Front Panel

    LAN Configuration TCP/IP Parameter Configuration Configuration Using Instrument Front Panel Configuration of the TCP/IP parameters via the front panel is the recommended method for setting up the module’s LAN interface. On-board configuration is only possible during the module’s start-up procedure. 1 Power on the detector, and press any front key when the following message is displayed “Press a key to enter communication...

  • Page 31: Configuration Using Web Browser

    LAN Configuration TCP/IP Parameter Configuration Configuration Using Web Browser This procedure describes how to change the TCP/IP parameters and hostname using a PC, via a Web browser. 1 Using a Web browser such as Internet Explorer, type in the Host name or IP address of the ELSD into the address bar (for example http://192.168.254.27).
  • Page 32 LAN Configuration TCP/IP Parameter Configuration 3 From the left menu, select Network to configure the TCP/IP settings. The detector can be configured to: • obtain an IP address automatically (DHCP), • use a static IP address, • change Hostname (Advanced Network settings). 4 To use a static IP address, select Use the following IP address.

  • Page 33: With Telnet

    LAN Configuration TCP/IP Parameter Configuration With Telnet Whenever a TCP/IP connection to the module is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. 1 Open the system (DOS) prompt window by clicking the Windows Start button and select Run..
  • Page 34 LAN Configuration TCP/IP Parameter Configuration 3 Enter the following information when prompted: • login:user • Password:Agilent The login details are case-sensitive. The password will be not visible on screen. NOTE 4 At the #> prompt type set network ? to view the available commands. Table 9 Telnet commands Value...
  • Page 35 LAN Configuration TCP/IP Parameter Configuration 5 To change a parameter, follow the style: Parameter value, for example: set network static=on set network ip=156.141.59.43 Then press Enter. Parameter refers to the configuration parameters that you are defining, and value refers to the definitions you are assigning to the parameter. Each entry is followed by a carriage return.

  • Page 36: Pc And Openlab Cds, Openlab Chemstation Or Openlab Ezchrom Software Setup

    LAN Configuration PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Software Setup PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Software Setup PC Setup for Local Configuration This procedure describes how to the change the TCP/IP settings on your PC to match the modules default parameters in a local configuration using a cross-over LAN cable (see Table 8...
  • Page 37 LAN Configuration PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Software Setup 2 Select Alternative Configuration. 3 Enter the following IP and subnet mask address for the PC: • IP address: 192.168.254.1 • Subnet mask: 255.255.255.0 4 Click OK to save the configuration. It can take several seconds for the alternative network settings to take effect.

  • Page 38: Agilent Chemstation Setup

    LAN Configuration PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Software Setup Agilent ChemStation Setup This procedure describes the Agilent OpenLab ChemStation setup for the 1290 Infinity II ELSD detector (G7102A) and 1260 Infinity II ELSD (G4260B) using LAN as the communication method. 1 Open the Instrument Configuration window.
  • Page 39 LAN Configuration PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Software Setup 4 Select IP Address and enter the module’s IP address. Select Hostname and enter the instrument’s serial number. Agilent InfinityLab ELSD User Manual...
  • Page 40 LAN Configuration PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Software Setup 5 Cooled option is selected by default. Therefore, deselect Cooled model option if required. 6 Select OK to complete the configuration. The detector’s serial number is the default hostname. NOTE If the detector is assigned a temporary IP address (e.g DHCP server), NOTE...

  • Page 41: Using The Module

    Using the Module Before Using the Detector Instrument Controls Display Screen Keypad Main menu bar Submenu Screen Status Mode Error conditions Clearing an Error Operational Parameters Method Evaporator Temperature Nebulizer Temperature Evaporator Gas Flow Detector Gain (PMT) Response Time (Smoothing) Light Source Intensity (LED) Power Mode Data Output Rate (Hz)

  • Page 42: Before Using The Detector

    Using the Module Before Using the Detector Before Using the Detector On start-up of both the Agilent 1260 Infinity II ELSD (G4260B) or the Agilent 1290 Infinity II ELSD (G7102A), either Serial or LAN must be selected as the method of communication.

  • Page 43: Instrument Controls

    Using the Module Instrument Controls Instrument Controls The ELS Detector can be used as a standalone detector via the front keypad and screen, as shown in Figure 7 on page 43 or via PC control using software (e.g. OpenLab CDS ChemStation). Display Screen The graphical interface on the front of the instrument displays the current method, status, evaporator temperature, nebulizer temperature, gas flow, and...

  • Page 44: Main Menu Bar

    Using the Module Instrument Controls Main menu bar To change the current settings, use the arrow keys to navigate across the interactive menu bar until the desired option is flashing. Using the up/down arrow keys to alter the parameter to the desired setting. When the cursor is located in the “Home”...

  • Page 45: Submenu Screen

    Using the Module Instrument Controls Submenu Screen The submenu screen is accessed from the front screen by selecting the ↓ key: This screen allows changes to the following electronic parameters: Set Signal Gain Smth Set Time Constant Set Light Source Intensity PwrMd Set Mode of ELSD when powered up Set Data Output Rate...

  • Page 46: Status Mode

    Using the Module Instrument Controls Status Mode The ELS Detector can be operated in two modes; STANDBY or RUN, both of which are described over page: To display the current mode and/or select a new mode, highlight the MODE function on the instrument display. The current mode will now be displayed on the screen.

  • Page 47: Error Conditions

    Using the Module Instrument Controls Error conditions The ELS Detector is equipped with several sensors and error checking facilities to ensure safe operation. If an error is detected, the instrument gives an audible warning and a visible description of the error condition. In event of any error condition, the unit defaults into the STANDBY mode in which the heaters, light source, and gas are turned off.

  • Page 48: Operational Parameters

    Using the Module Operational Parameters Operational Parameters Method The ELS Detector has 10 onboard preset methods. These methods comprise evaporator and nebulizer temperatures and gas flow, which can be optimized for specific applications. These 10 onboard methods are selected using the front keypad and screen, via the METHOD option.

  • Page 49: Nebulizer Temperature

    Using the Module Operational Parameters Nebulizer Temperature The nebulizer temperature can be used to optimize signal response in addition to evaporator temperature. Higher nebulizer temperatures increase peak response, but the nebulizer temperature must not exceed the boiling point of the mobile phase.

  • Page 50: Response Time (Smoothing)

    Using the Module Operational Parameters Response Time (Smoothing) The data outputted from the detector can be averaged to produce a smoother response. The smoothing width is set to the number of data points over which the data is averaged and can be regarded as a digital time constant. The smoothing range is settable from 1 –...

  • Page 51: Data Output Rate (Hz)

    Using the Module Operational Parameters Data Output Rate (Hz) The rate at which the ELS Detector outputs data can be selected from the submenu screen. A 10 Hz output rate is selectable for standard LC applications, a 40 Hz output rate can be chosen for faster LC separations, and 80 Hz is used for UHPLC type applications with very narrow peaks.

  • Page 52: Controlling The Elsd During An Injection

    Using the Module Controlling the ELSD During an Injection Controlling the ELSD During an Injection The ELS Detector can change operational parameters in real time, during a sample injection, using an onboard timetable. Real-Time Operation The ELS Detector can store in memory a series of time-based events, within a single timetable.

  • Page 53: Starting And Stopping Real-Time Control

    Using the Module Controlling the ELSD During an Injection Starting and Stopping Real-Time Control The ELSD contains an internal timer to initiate the time-based events stored within the timetable. To start the internal timer and trigger the on-board timetable, a contact closure input via the I/O connector on the rear of the instrument is required (see “Overview of ELS Detector”...

  • Page 54: Controlling The Elsd Within Openlab Cds, Openlab Chemstation Or Openlab Ezchrom

    Using the Module Controlling the ELSD within OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom Controlling the ELSD within OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom The ELS Detector can be controlled directly using OpenLab CDS, OpenLab ChemStation (ChemStation Rev. B not supported), or OpenLab EZChrom. Digital data acquisition is performed without the need for an A/D interface.

  • Page 55: General Considerations

    Using the Module General Considerations General Considerations The ELS Detector should be thought of as a detector like any other designed for liquid chromatography. The main distinguishing feature is the ability to evaporate the solvent from the column eluent. Therefore, normal system setup precautions should be remembered when starting to use the instrument.

  • Page 56: Solvent Recommendations

    Using the Module General Considerations Solvent Recommendations Any solvent that is intended for use with the ELS Detector should be thoroughly degassed, filtered (0.45 µm) and fully compatible with the columns. Solvents that are not properly degassed may cause problems at nebulization leading to a poor reproducibility.
  • Page 57 Using the Module General Considerations Table 10 Volatile mobile additives compatible with ELS detection Mobile Phase Additive pH Range Bp (°C) Mp (°C) Acids Trifluoroacetic Acid (TFA) 13.70 72.4 -15.4 Formic Acid 3.75 10.25 100.7 Acetic Acid 4.75 9.25 116.0 16.6 Bases Ammonia...

  • Page 58: Transferring Elsd Temperature Methods

    Using the Module General Considerations Transferring ELSD Temperature Methods The direct transfer of ELSD operating conditions from other manufacturers’ ELS detectors, or other designs of ELSD (e.g. Agilent G4218A ELSD) to the ELSD will not provide equivalent performance. As stated in “Basic Principles of Operation”...

  • Page 59: Optimizing Performance

    Optimizing Performance Do’s and Don’ts of ELS Detection Location of the Detector Module Pumping Systems Mobile Phase Priming This chapter gives hints on how to optimize the performance or use additional devices. Agilent InfinityLab ELSD User Manual...

  • Page 60: Do's And Don'ts Of Els Detection

    Optimizing Performance Do’s and Don’ts of ELS Detection Do’s and Don’ts of ELS Detection Decreased performance C AU T I O N High pressures on the internal chamber will lead to increased baseline noise and low sensitivity.  NEVER block the exhaust outlet. NEVER allow the solvent waste outlet tube to become immersed in the ...

  • Page 61: Location Of The Detector Module

    Optimizing Performance Location of the Detector Module Location of the Detector Module Place the detector conveniently near your HPLC system. The modular design of the ELSD enables you to locate it anywhere within the limitations imposed by the length of the power cord, fluid lines, and signal cables. To keep liquid dead volume as low as possible and to minimize peak broadening in the lines, the distance between the column outlet and the flow cell inlet should be kept to a minimum.

  • Page 62: Pumping Systems

    Optimizing Performance Pumping Systems Pumping Systems It is recommended to use a high-performance pumping system with no flow pulses to minimize nebulization problems. Inconsistent solvent flow will result in poor reproducibility. A backpressure regulator maybe necessary on certain pumps to minimize pulsation.

  • Page 63: Mobile Phase Priming

    Optimizing Performance Mobile Phase Priming Mobile Phase Priming The ELS detector does not require any mobile phase priming. It is recommended that priming of the LC system is performed without the ELS detector attached to prevent nonvolatile impurities contaminating the ELS detector. The mobile phase should be degassed and filtered, either by sparging with Helium or using an online degasser.

  • Page 64: Troubleshooting And Diagnostics

    Troubleshooting and Diagnostics Troubleshooting Troubleshooting an HPLC System General Problems Baseline noise Baseline spikes Low sensitivity Spiky peak tops but flat baseline Large Baseline offset Peak tailing Instrument Fails to zero No power No response (completely flat baseline) Temperature error as soon as instrument powered on Display not on, but power connected Cooled Evaporator Temperature reads zero at start-up and cannot be changed Vapor sensor error occurs, but there is no solvent or vapor leak inside unit...

  • Page 65: Troubleshooting

    Troubleshooting Troubleshooting If a problem is encountered, Agilent Technologies advises you to first follow the troubleshooting section to resolve the problem. If there is an error or fault and you follow the recommended course of action but the result is not satisfactory, then direct the matter to Agilent Technologies or your local distributor.

  • Page 66: Troubleshooting An Hplc System

    Troubleshooting and Diagnostics Troubleshooting an HPLC System Troubleshooting an HPLC System Standard practice is to add one component at a time back into the HPLC system so that the component causing the problem is easily identified if/when the condition re-occurs. Begin troubleshooting by adding the pump to the ELS Detector first and finish by adding the column last.

  • Page 67: General Problems

    Troubleshooting and Diagnostics General Problems General Problems Baseline noise Probable cause Suggested actions Increase the temperature of the nebulizer by Poor nebulization 10 °C until the baseline noise decreases. • Increase the temperature of the evaporator Insufficient evaporation by 10 °C until the baseline noise decreases. •...

  • Page 68: Baseline Spikes

    Troubleshooting and Diagnostics General Problems Baseline spikes Probable cause Suggested actions Filter the incoming gas, or change the supply. Particulate matter in the gas supply Replace column or fit an inline filter with a 0.2 µm Column shedding membrane filter directly after the column. •...

  • Page 69: Spiky Peak Tops But Flat Baseline

    Use a lower concentration of stabilizer, High concentration of nonvolatile buffer or unstabilized solvent or, a more volatile buffer stabilizer (ammonium acetate or ammonium formate). Perform cleaning procedure. Contaminated diffuser Refer to local distributor or Agilent Technologies. Optics Heater failed Agilent InfinityLab ELSD User Manual...

  • Page 70: Peak Tailing

    Troubleshooting and Diagnostics General Problems Peak tailing Probable cause Suggested actions Increase evaporator gas flow rate. Eluent particles lingering in the optical chamber Optimize HPLC separation. Poor chromatography Instrument Fails to zero Probable cause Suggested actions • Stop pump flow and switch off unit. Restart Offset too high or output unstable due to unit and A/Z without liquid flowing.

  • Page 71: No Response (Completely Flat Baseline)

    Temperature error as soon as instrument powered on Probable cause Suggested actions • Check RTD connections. Temperature probe fault or disconnected • Consult Agilent Technologies or your local agent for further advice. Agilent InfinityLab ELSD User Manual...

  • Page 72: Display Not On, But Power Connected

    Troubleshooting and Diagnostics General Problems Display not on, but power connected Probable cause Suggested actions Refer to local distributor or Agilent Technologies. Instrument Power Supply Refer to local distributor or Agilent Technologies. Faulty display Cooled Evaporator Temperature reads zero at...

  • Page 73: Cooled Evaporator Will Not Reach Low Temperature E.g. 10 °C

    Probable cause Suggested actions Move detector to laboratory where ambient Ambient lab temperature too high temperature is <25 °C. Consult Agilent Technologies or your local agent Faulty Peltier cooler unit if problem persists. High backpressure from detector Probable cause Suggested actions...

  • Page 74: Error Information

    Error Information What Are Error Messages Module Specific Error Messages Internal temperature exceeded lower limit Internal temperature exceeded upper limit On-board Vapor sensor failed Rear Vapor sensor failed Vapor detected Leak detected Fan Failed Fan Stopped Nebulizer temperature limit exceeded Evaporator temperature limit exceeded LED or Laser light source error Evaporator gas flow rate limit exceeded...

  • Page 75: What Are Error Messages

    Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).

  • Page 76: Module Specific Error Messages

    Error Information Module Specific Error Messages Module Specific Error Messages These errors are detector-specific. Internal temperature exceeded lower limit Error ID: 10 Air temperature inside the instrument is ≤10 °C Probable cause Suggested actions • Increase ambient temperature where The environmental temperature is outside detector is located.

  • Page 77: Rear Vapor Sensor Failed

    Error Information Module Specific Error Messages Rear Vapor sensor failed Error ID: 13 The vapor sensor located on the rear panel of the module has failed. Probable cause Suggested actions Please contact your Agilent service Vapor sensor not connected to the representative.

  • Page 78: Fan Failed

    Error Information Module Specific Error Messages Fan Failed Error ID: 16 Thermal shut-down of the main cooling fan. Probable cause Suggested actions Please contact your Agilent service representative. Fan cable disconnected. Please contact your Agilent service representative. Defective fan. Please contact your Agilent service representative. Defective mainboard.

  • Page 79: Evaporator Temperature Limit Exceeded

    Error Information Module Specific Error Messages Evaporator temperature limit exceeded Error ID: 19 Evaporator temperature exceeded threshold after stabilizing. Probable cause Suggested actions Please contact your Agilent service representative. Defective thermocouple. Please contact your Agilent service representative. Defective evaporator heater assembly. Please contact your Agilent service representative.

  • Page 80: Invalid Nebulizer Temperature

    Error Information Module Specific Error Messages Invalid Nebulizer temperature Error ID: 22 Invalid nebulizer temperature reading. Probable cause Suggested actions Please contact your Agilent service representative. Nebulizer heater not connected to the mainboard. Please contact your Agilent service representative. Defective nebulizer heater. Invalid Evaporator temperature Error ID: 23 Invalid evaporator temperature reading.

  • Page 81: Cooled Evaporator Current Out Of Range Or Communication Failed

    Error Information Module Specific Error Messages Cooled evaporator current out of range or communication failed Error ID: 25 Peltier module current outside of normal range or communication to Peltier module has failed. Probable cause Suggested actions Please contact your Agilent service representative. Defective Peltier assembly.

  • Page 82: Laser Interlock Open Or Failed

    Error Information Module Specific Error Messages Laser interlock open or failed Error ID: 28 The laser interlock on the detector enclosure is open or failed. Probable cause Suggested actions Please contact your Agilent service representative. Module enclosure is open. Please contact your Agilent service representative. Interlock not connected to mainboard.

  • Page 83: Maintenance

    Maintenance Introduction to Maintenance Cautions and Warnings Cleaning the Module Inspection of Cables Drying the Diffuser Cleaning the Nebulizer Cleaning Evaporator Tube Putting the Instrument into Storage Updating Detector Firmware This chapter describes the maintenance of the ELSD. Agilent InfinityLab ELSD User Manual...

  • Page 84: Introduction To Maintenance

    Maintenance Introduction to Maintenance Introduction to Maintenance Trained personnel only should carry out maintenance inside the unit. There are no user serviceable parts inside the instrument. Unauthorized access to the instrument will invalidate the instrument warranty. Information for Service Personnel Please note that this instrument is double fused.

  • Page 85: Cautions And Warnings

    Maintenance Cautions and Warnings Cautions and Warnings The module is partially energized when switched off, as long as the power WAR N IN G cord is plugged in. Repair work at the module can lead to personal injuries, e.g. electrical shock, when the cover is opened and the module is connected to power.
  • Page 86 Maintenance Cautions and Warnings Fire and damage to the module WAR N IN G Wrong fuses Make sure that only fuses with the required rated current and of the  specified type (super-fast, fast, time delay etc) are used for replacement.

  • Page 87: Cleaning The Module

    Maintenance Cleaning the Module Cleaning the Module The exterior of the instrument should be cleaned by wiping down with a soft cloth moistened with dilute detergent solution, followed by wiping down with a cloth moistened with de-ionized water. Ensure that no moisture enters the instrument. Electrical shock and burns WAR N IN G Liquid in the module electronics can cause shock hazard and damage the...

  • Page 88: Inspection Of Cables

    Maintenance Inspection of Cables Inspection of Cables Periodically inspect the connecting cables for signs of physical damage caused by abrasion, solvent spillage, impact etc. Replace damaged cables, particularly the power cord, if any damage is observed. Agilent InfinityLab ELSD User Manual...

  • Page 89: Drying The Diffuser

    Maintenance Drying the Diffuser Drying the Diffuser If the instrument has been operated incorrectly, the diffuser may become blocked with liquid. This is manifested by loss of signal, increased baseline noise and in the extreme case gas bubbling out of the nebulizer drain tube. If this happens, proceed as follows: 1 Increase the gas flow to 2.8 SLM and increase the evaporator to maximum temperature.

  • Page 90: Cleaning The Nebulizer

    Maintenance Cleaning the Nebulizer Cleaning the Nebulizer A loss of sensitivity is a common indicator that the nebulizer requires cleaning. Flushing can remove blockages. Therefore it is recommended to initially flush the instrument with a suitable solvent (for example water). The most common cause of nebulizer blockage is precipitation of mobile phase buffer.

  • Page 91: Cleaning Evaporator Tube

    40 h of use as a preventive routine. It is also recommended to clean the unit following the use of buffers. If cleaning the unit does not solve the problems, consult Agilent Technologies for further assistance. Do not use solvents that contain additives when performing the cleaning NOTE procedure.

  • Page 92: Putting The Instrument Into Storage

    Maintenance Putting the Instrument into Storage Putting the Instrument into Storage If the instrument is to be stored or not used for an extended period, it is recommended to follow this procedure: 1 Flush the detector with a mixture of Acetone/Water (50/50) at 1 mL/min for 15 min.

  • Page 93: Updating Detector Firmware

    Maintenance Updating Detector Firmware Updating Detector Firmware The ELSD Firmware can be upgraded using Lab Advisor. Where Lab Advisor is not available, the following process may be used. The Agilent 1290 Infinity II ELSD (G7102A) also contains additional firmware on the Peltier unit and the Laser assembly.
  • Page 94 Maintenance Updating Detector Firmware 3 Select Serial and choose the appropriate COM port, followed by OK. 4 From the toolbar menu, select Setup >Serial Port to configure the serial port connection. 5 Set the Serial port to the parameters shown below and click OK. The Transmit delay values should be set according to the table below: Table 11 Transmit delay values...
  • Page 95 Maintenance Updating Detector Firmware 6 Put the detector into Service mode by holding down the rear Flash button whilst switching on the module. The front panel will display: SERVICE MODE Reboot for normal operation When in Service mode, the Tera Term displays the bootloader version and firmware menu.
  • Page 96 Maintenance Updating Detector Firmware 9 To choose the firmware file, select the Send File option from the File menu in the toolbar and navigate to the folder where the file is located. 10 Select the firmware hex file and click Open. The download will begin straightaway and progress will be displayed, as shown.
  • Page 97 Maintenance Updating Detector Firmware 11 On successful completion of the file transfer, the software will display a Flash memory updated ok message. 12 Power cycle the module and check that the firmware version displayed on the ELSD front panel at boot-up is correct. Agilent InfinityLab ELSD User Manual...

  • Page 98: Parts And Materials For Maintenance

    Parts and Materials for Maintenance Identifying Parts and Materials This chapter provides information on parts for maintenance. Agilent InfinityLab ELSD User Manual...

  • Page 99: Identifying Parts And Materials

    Parts and Materials for Maintenance Identifying Parts and Materials Identifying Parts and Materials Description G4260B Agilent 1260 Infinity II Evaporative Light Scattering Detector OR G7102A Agilent 1290 Infinity II Evaporative Light Scattering Detector PL0890-0305 Gas inlet tube (5 m) PL0890-0310 Rear exhaust hose (PVC-2 m) PL0890-0315 Solvent waste tube (2 m)

  • Page 100: Appendix

    Appendix General Safety Information Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Agilent Technologies on Internet This chapter provides addition information on safety, legal, and Web. Agilent InfinityLab ELSD User Manual...

  • Page 101: General Safety Information

    Appendix General Safety Information General Safety Information Safety Symbols Table 12 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user shall refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.
  • Page 102 Appendix General Safety Information Table 12 Safety Symbols Symbol Description Power symbol indicates On/Off. The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position. Pacemaker A pacemaker could switch into test mode and cause illness. A heart defibrillator may stop working.
  • Page 103 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
  • Page 104 Appendix General Safety Information Operation Before applying power, comply with the installation section. Additionally the following must be observed. Do not remove instrument covers when operating. Before the instrument is switched on, all protective earth terminals, extension cords, auto-transformers, and devices connected to it must be connected to a protective earth via a ground socket.

  • Page 105: Waste Electrical And Electronic Equipment (Weee) Directive

    Appendix Waste Electrical and Electronic Equipment (WEEE) Directive Waste Electrical and Electronic Equipment (WEEE) Directive This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste NOTE To return unwanted products, contact your local Agilent office, or see...

  • Page 106: Radio Interference

    Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Agilent InfinityLab ELSD User Manual...

  • Page 107: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: https://www.agilent.com Agilent InfinityLab ELSD User Manual...
  • Page 108 The manual describes the following: • Introduction • Site Requirements and Specifications • Using • Optimizing • Troubleshooting and Diagnostics • Error Information • Maintenance • Parts • Safety www.agilent.com  Agilent Technologies Inc. 2022 Edition: 08/2022 Document No: D0013647 Rev. A...

Table of Contents