Cellink BIO X6 User Manual page 91

4.6 BIO X6 Protocol Parameter Guidelines
4.6.1 BIO X6 Protocol Parameter Guidelines Protocol aim
The aim of this protocol is to provide instructions on how to improve printed structures.
Illustrations will provide clarity on how to adjust the print settings and how it will affect the
print. At the beginning of the document is an explanation of what the different parameters in
DNA Studio are.
4.6.2 Background
The basic settings in the DNA Studio printhead tab are printhead, nozzle type, pressure, print
speed, temperature, External pressure pump. By modifying these values, improvements to
the print can be made. Below you can find common issues and how these parameters can
influence your print.
4.6.3 Parameters in DNA Studio
Nozzle type:
The nozzle type includes the size of the nozzle/needle inner diameter. Changing the nozzle
type will result in thinner or thicker printed lines. It is recommended to use a nozzle type
between 16–30 G.
Pressure:
Pressure flow determines material flow out of the nozzle type (16–30 G). (Pressure should
be between 0–200 kPa with the internal air compressor and between 0–700 kPa with an
external air compressor). Extrusion pressure depends mainly on the viscosity and rheological
properties of the material to be printed.
Speed:
The speed of the printhead during printing moves. Increasing the printing speed will result in
a shorter printing time while decreasing the speed can give higher resolution of the printed
structures. It is not recommended to use a speed higher than 0–10 mm/s.
Temperature:
BIO X6 User Manual v. 1.0 91