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（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex product. Shodex Asahipak NH2P series is a polymer-based
chromatography column modified with amino group. The column is suitable for the analyses that require HILIC
or ion exchange mode separations.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F7630006
Asahipak NH2P-50 2D
F6713000
Asahipak NH2P-50G 2A
Base Material
: Spherical porous particles of polyvinyl alcohol modified with amino group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Acetonitrile
Operation Manual
Shodex
Asahipak
Column Size (mm)
I.D.
2.0
2.0
SHOWA DENKO K.K. (https://www.shodex.com/)
NH2P-50 2D
Particle
Size (μm)
Length
150
5
10
5
ver.211102E
Theoretical Plate Number
(Per Column)
≥ 3,500
(Guard Column)
1
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Summary of Contents for Shodex Asahipak NH2P-50 2D
- Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex Asahipak NH2P series is a polymer-based chromatography column modified with amino group. The column is suitable for the analyses that require HILIC or ion exchange mode separations.
- Page 2 Pressure (MPa) pH Range Range (°C) Recommended Maximum (Per Column) Asahipak NH2P-50 2D 0.1 - 0.2 2 - 13 4 - 50 Asahipak NH2P-50G 2A Usable solvents are listed below. (1) A mixture of water and acetonitrile or water and ethanol of any ratio can be used. Up to 100 % water, acetonitrile, or ethanol can be used.
- Page 3 This may result in creating an empty space in the column and deteriorates the column. Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 4 Method 2: Adsorption of metal ions or in case Method 1 did not work Washing Solution Cleaning Time 6 min 0.1 M HNO aq. solution 75 min 6 min 0.1 M NaOH aq. solution 75 min 12 min Eluent 40 min SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
- Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.
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