Shodex ODP2 HP-4B Operation Manual

(Please read this manual carefully to achieve the best and consistent column performance for a long time)

Important Handling Instructions

Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.

Before Using the Column

(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex ODP2 HP series is polymer-based reversed phase
chromatography columns which retain hydrophilic compounds stronger than ordinarily ODS columns do. The
small pore size of the packing material excludes proteins fast, and this makes the columns suitable for
separating small-molecular weight components, such as drugs, even in the presence of protein.
The column also works as a hydrophobic interaction chromatography (HIC) column by using high-concentration
ammonium sulfate aqueous solution as an eluent.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F7622001 ODP2 HP-4B
F7622002 ODP2 HP-4D
F7622003 ODP2 HP-4E
F6714010 ODP2 HPG-4A
Base Material : Spherical porous particles of polyhydroxymethacrylate
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile = 55/45
Operation Manual

Shodex ODP2 HP-4B, 4D, 4E
Column Size (mm)
I.D.
4.6
4.6
4.6
4.6
SHOWA DENKO K.K. (https://www.shodex.com/)
Particle
Size (μm)
Length
50 5
150 5
250 5
10 5
ver.211124E
Theoretical Plate Number
(Per Column)
≥ 3,500
≥ 10,000
≥ 17,000
(Guard Column)
1