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a D:GROUP Company
7.B
INSTRUCTIONS FOR THE FUMIGATION/STERILIZATION OF BIOLOGIC SAFETY
CABINETS
Procedure:
For gas disinfection, formaldehyde is evaporated in the tightly sealed sample chamber. The quantity of the
formaldehyde used depends on the sample chamber volume of the cabinet version to be disinfected (see
Technical Data).
Per cubic meter of sample chamber volume, at least 5 g formaldehyde must be evaporated with 20 ml water
(corresponds with 25 ml of a 20 % formaldehyde solution). The formaldehyde evaporates immediately when
its boiling point is reached. The required reaction time is at least 6 hours.
After the required reaction time, the formaldehyde should be neutralized by evaporating a 25 % ammonium
solution (10 ml per cubic meter of sample chamber volume).
Ambient conditions and accessories:
The temperature at the cabinet location should be approx 21° C, the relative
humidity should be between 60 and 85 %. To evaporate the solution, a heating
device with a container is required.
NOTE – Disinfection procedure!
A disinfection with formaldehyde must be performed in accordance with the specifications
of NSF 49/1992, Annex G. As this procedure has considerable risks, it must only be performed by
specially trained and authorized service personnel!
If you use a FASTER formalin vaporizer follow this propcedure:
1. after filling the two containers of the vaporizer according to the above-said instructions, the vaporizer can
be connected to the power point inside the cabinet.
2. set the timer at 6 hours.
3. cover the cabinet completely with a polyethylene sheet which is to be carefully sealed at the bottom with
adhesive tape (see enclosed picture 1); also the feeding cable of the cabinet must be sealed as shown in
the enclosed picture 2.
4. start the sterilization cycle by pressing SET and STATUS key together, digit the password (3 times arrow
up + ENTER). All the functions of the cabinet are deactivated except the power point. Now the fans start
working for 30 secs. at intervals of 15 min. for totally 8 hours.
CYCLE: abt. 1 hour of formaldehyde evaporation, 6 hours (time set with the timer) of fumigation process,
1 hour of reagent evaporation. Total time : 8 hours.
5. when the sterilization cycle is over, the message "END STERILIZATION" is displayed
6. to go out of the programme press SET and STATUS key together, digit the password (3 times arrow up +
ENTER)
7. At this point, after wearing protective gloves, protective musk and clothing the polyethylene sheet can be
removed and must be submitted to special waste disposal according to possible local regulations.
8. Aerate profusely the room in order to evacuate completely the residual gas and smell.
This fumigation operation is to be considered as a suggestion.
00055EN Rev.10 – 12/2011
23
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