Related Manuals for Cole Parmer 1200 Series
Summary of Contents for Cole Parmer 1200 Series
- Page 1 Cole Parmer 1200 SERIES SPECTROPHOTOMETER new version) USER’S MANUAL Cole Parmer Instruments Company 625 East Bunker Court Vernon Hills, IL 60061 800-232-4340...
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Page 2: Table Of Contents
Table of Contents General Information ………………………………………………………………………………… Introduction ………………………………………………………………..........Working Principle …………………………………………………………………………………..Specifications ......…………………………………………………………………………… Unpacking Instructions …………………………………………………………………………… Installation …………………………………………………………………………………………………… 1200 and 1201 Spectrophotometer Operation Panel ……………………... Changing Curette Holders ……………………………………………………………………..Basic Operation ………………………………………………………………………………………... Sample Preparation and Analysis ………………………………………….….… Concentration Mode …….……….……………………………………………….…… Factor Mode …………………………..……….…………………………………………. -
Page 3: General Information
General Information The spectrophotometer described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory. For the correct and safe use of this spectrophotometer it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual. -
Page 4: Introduction
Radio Interference For compliance with the EMC standards referred to in the EC Declaration of Conformity, it is necessary that only shielded cables supplied by Cole Parmer are used when connecting the instrument to computers and accessories. Introduction The newly redesigned Cole Parmer 1200/1201 Spectrophotometers are single beam, general purpose instruments designed to meet the needs of the conventional laboratory. -
Page 5: Specifications
In the spectrophotometer, light from the Halogen Lamp is focused on the entrance slit of the Monochromator where the collimating mirror directs the beam onto the grating. The grating disperses the light beam to produce the spectrum, a portion of which is focused on the exit slit of the Monochromator by a collimating mirror. -
Page 6: Unpacking Instructions
3. Select either 230V or 125V on the Voltage Selector Switch on the bottom of the 1200/1201 shown in Figure-2, to match your local main voltage supply. 4. Turn on your Cole Parmer 1200/1201 and allow it to warm up for 15 minutes before taking any readings. -
Page 7: 1200 And 1201 Spectrophotometer Operation Panel
0A/100%T Print/Enter Sample compartment Wavelength Knob Figure-3 Cole Parmer 1200 Spectrophotometer MODE Button: There are four modes. T mode is transmittance mode; A mode is absorbance mode; C mode is to measure unknown sample concentration through a standard solution; F mode is to measure unknown value with a previously determined factor. -
Page 8: Changing Curette Holders
Windows-based Application Software, the ® communication with the PC will be set up (refer to the UNICO 1200 Series Spectrophotometer Software User’s Manual Version SS-1.22 for details). Sample Compartment: Accept 10 mm test tube or 10 mm square cuvette (with different cuvette holders). - Page 9 Figure-5 4-position Cuvette Holder with Base Step 1 Remove 4-position Cuvette Holder Rod. Unscrew the 4-position Cuvette Holder Rod by turning the knob counterclockwise until it was removed from the Holder. Gently slide the guide rod out of the Sample Compartment. Step 2 Locate the Front Screw of the Holder Base.
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Page 10: Basic Operation
Basic Operation Simple OPERATION INSTRUCTIONS are printed on the front panel of your Cole Parmer 1200/1201. Three Basic Operations: Sample Preparation and Analysis, Concentration Mode-C, and Factor Mode are shown in this section. Sample Preparation and Analysis Spectrophotometer Warm-up and 0%T Check Turn on the spectrophotometer by turning on the Power Switch (IO). -
Page 11: Concentration Mode
Concentration Mode C is used for determining the concentration of unknown samples. NOTE: This method should only be used when the relationship between Absorbance and Concentration is known to be linear. The concentration of the Standard solution used to calibrate the instrument should be higher than the most concentrated sample. 1. -
Page 12: Output And Data Processing
Printer Model 1200/1201 Model has a RS232C Interface connector that can be connected to any RS232 printers (Cole Parmer provides RS232 printer, item number: S-1100-206, as an optional accessory). It requires a 9-pin, null modem connection cable. The RS232 Printer Setting should be as follows: •... -
Page 13: Maintenance
Refer to the 1200 Series Spectrophotometer Software User’s Manual Version SS- 1.22 for detail. Maintenance Lamp Replacement 1. Turn off and unplug the instrument. 2. Remove the lamp access door at the back of the instrument by removing the four set screws. -
Page 14: Absorbance Accuracy Check
flashes. The reading should be 0.000A. If not, repeat step 5. 6. Remove the Cuvette Adapter and insert the Holmium filter into it. Place it in the Sample Compartment again and close the lid. 7. Record the Absorbance reading on the Digital Display window. 8. -
Page 15: Stray Light Check
PSG Bulletin 1967, No. 17, page 505 Make up N/100 sulfuric acid as the solvent and use part of it to make a solution containing 120 + 0.5 mg/l of potassium dichromate. Wash out a square cuvette with solvent, and fill with solvent. Put the cuvette in the Cuvette Adapter, then into the Sample Compartment and close the lid. - Page 16 Table-3 1200 and 1201 Parts List Item Number Description S-1200 Model 1200 (new) Spectrophotometer 5 nm Bandpass Wavelength range: 325~1000 nm. Voltage preset at 110V Complete with 10 mm single Square Cuvette Holder, V-type Holder for 5~10 mm round tube, a pair of Glass Cuvettes, 12 pcs.
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Page 17: Troubleshooting
Troubleshooting Table-4 Troubleshooting PROBLEM Possible Cause Solution Power cord not connected to outlet Plug instrument in Instrument Inoperative Dead Power outlet Change to a different outlet Wrong voltage setting (Power indicator has no light) Internal fuse blown or defective electronic Call an authorized service component engineer... - Page 18 Instrument drift No sufficient warm up time Check lamp has been properly Significant temperature change installed or has moved during and noise Lamp not adjusted properly transit Refer to Lamp Replacement instructions in this manual Lamp old or defective Replace with a new lamp Sample Holder Misaligned Refer to Lamp Replacement instructions in this manual...
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