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1.1.1 Operation and Maintenance Manual Electronic Laboratory Autoclaves Models LABSCI 11LV First time published; 06.22.2014 Current revision (G) published 01.17.2016 ELV-D: standard autoclave C: optional system for fast cooling PV: optional vacuum pump BH: optional bio hazard filtration WR: water recycling system F: optional fan for super-fast cooling Cat.
Table of Contents Paragraph page no. 1. GENERAL Available optional configurations ............... 5 Incoming Inspection ..................6 Warranty ...................... 6 Warranty Statement ..................7 Accessories ....................7 Safety Instructions ....................8 Technical Data ....................10 Operating Conditions ................. 10 Directives and Standards ................10 Ordering Information ................
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Program 6: Liquid B – Waste Cooling (C – cooling models only) .... 37 Program 7: Bio Hazard 1 (BH, bio-hazard models only) ......38 Program 8: Bio Hazard 2 (BH, bio-hazard models only) ......39 Program 9: Bio Hazard Liquids (BH, bio-hazard models only) ....40 5.10 Program 10: Vacuum Test (PV, pre-vacuum models only) ......
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10.11 Loading and Unloading the Device ............78 Service and Maintenance ................... 80 11.1 Preventive Maintenance ................80 11.2 Checking the Safety Valve ................. 81 Troubleshooting ....................84 Baskets and Containers ..................89 Spare Parts List ....................89 Accessories ......................90 Page 3...
1. Generals 1.1 Introduction Models LABSCI 11LV ELV/ELVC/ELVPVBH are vertical sterilizers designed especially for sterilization of instruments, liquids and other materials in hospital laboratories, laboratories & research institutes, food laboratories and pharmaceutical facilities. The sterilizer is fully automatic with a choice of ten programs (including two custom programs), eliminating any need for operator intervention during a cycle.
Only technical personnel having proper qualifications, holding technical documentation and adequate test instrumentation, are authorized to undertake repair or service. 1.2 Available optional configurations ELV is the basic model of the vertical laboratory autoclave. A special feature of the C models is the fast cooling stage for liquids. In this stage pressure in the chamber is increased by means of compressed air to compensate the fast decreasing of pressure due to the fast cooling.
All Tuttnauer products are carefully inspected prior to shipment and all reasonable precautions are taken in preparing them for shipment to assure safe arrival at their destination.
Troubleshooting, Service incidents, Warranty repair, Repair, Services and Return authorization : 224-265-9600 Fax: 224-265-9611 e-mail: service@heidolph.com Tuttnauer USA Co., Ltd., 25 Power Drive Hauppauge, NY 11788, USA : (800) 624 5836, (631) 737 4850, Fax: (631) 737 0720 e-mail:info@tuttnauerUSA.com. Note: If there is any difficulty with this instrument, and the solution is not covered in this manual, contact our representative or us first.
2. Safety Instructions The autoclave has unique characteristics. Please read and understand the operation instructions before first operation of the autoclave. The following issues may require instructions guidance provided by the manufacturer: how to operate the autoclave, the door safety mechanism, the dangers involved in circumventing safety means, how to ensure that the door is closed, and how to select a correct sterilization program.
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On closing the autoclave's door, make sure it is properly locked before activating. Before withdrawing baskets, wear heat resistant gloves. Before opening the door, verify that there is no pressure in the chamber (chamber pressure gauge is located on the autoclave's front panel or door, depends on model).
Tuttnauer. Ltd. company meets the provisions of the following standards: 9001:2008 (Quality Systems) Tuttnauer. Ltd. company also works in conjunction with and refers to: ANSI/AAMI ST55 American Society of Mechanical Engineers Section VIII, Division 1, for unfied pressure vessels. EN 13060 Small Steam Sterilizers.
We certify that this instrument is guaranteed to be free from defects in material and workmanship for one year against faulty components and assembly with the exception of glassware, lamps and heaters. The warranty does not include and does not replace routine treatment and preventive maintenance to be performed according to instructions in sec.
3.5 Environment Emission Information Peak sound level generated by the sterilizer is « 70 / dBA with a back sound level of 60 dBA. Total heat transmitted by the sterilizer is < 240 W/h 3.6 Electrical Data Property Value Total Power 2400W (2*1200W) Voltage 230VAC/1Ph...
Compliance with the above data should be tested in accordance with acknowledged analytical methods, an authorized laboratory. Attention: We recommend testing the water quality once a month. The use of water for autoclaves that does not comply with the table above may have severe impact on the working life of the sterilizer and can invalidate the manufacturer’s guarantee.
• The electrical network must comply with local rules or regulations. • Verify that there is an easy access to the main power switch, to the water cut-off valve and to the current leakage safety relay. • Make sure while placing the autoclave, to leave space around the machine, to give the technician access to service the machine.
3.12 Front View Description Description Door handle Printer Door cover USB socket Right service door Display Right service door grip Keyboard Front leg Main switch Front service cover Printer cover Ventilation grill Right service door lock Page 16...
1.1. Front View: What is Special for WR Configuration Description Description Glass tube indicator of the Inlet for manual water reservoir water level reservoir filling Wheel Page 17...
3.13 Rear View No. Description No. Description Air pressure regulator (C – cooling models Fan grill only) Air pressure gauge (C – cooling models only) Left service door grill Compressed air inlet (C – cooling models Left service door lock only) Mineral free water inlet RJ45 connector...
3.16 Overall Dimensions, WR Configuration(mm) 3.17 Safety Features This autoclave includes built-in safety features such as: Error message display. Electronic pressure and temperature measurement. Safety relief valve to avoid build-up of excessive pressure. Door switches enabling operation to be started only when the door is closed.
3.18 Description of Operation 3.18.1 Heat The ELV vertical autoclaves are equipped with immersion type, heating elements. After water has been introduced to the chamber and the unit has been activated, the heating elements begin to heat. The temperature and pressure in the chamber increase until appropriate levels are reached.
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When the timed sterilization cycle is complete, the unit enters into the exhaust stage, provided that a program other than the liquid program was selected. The steam is exhausted from the chamber, bringing the internal pressure down to atmospheric pressure. Page 23...
4.2 Description and Functions of the Front Panel Keyboard The front panel is composed of 3 sections: Display screen. Keypad. Printer 4.2.1 Display screen The display is a LCD panel used to display the current status of the autoclave while using Operational Messages and Error Messages. Program description: Program icon and name •...
on “SET” or “EXIT” the UP ▲ key activates that procedure.” DOWN key This key has the following functions: • In the menu directories: This key enables the operator to browse through the cycles. • In the directories available: When the cursor is blinking on a number, the DOWN ▼ key decreases its value.
Failure that occur after completing the sterilization stage, which in this case will leave the load sterilized For the list of Displayed Error Messages / Symbols see sec. 13. TROUBLESHOOTING 4.4 Displayed operational messages / Symbols Message / Message / Symbol Required Action Symbol Name Description...
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This message is displayed if Perform a new cycle to the electrode in the chamber drain the chamber. senses water. Supplied Mineral free water reservoir Fill the mineral free Distilled Water empty" water reservoir. Error Page 28...
5. Sterilization Programs Sterilization Programs Temp. models models models only only only Icon Description 134°C Glass (273F) 121°C Plastic (250F) 121°C Liquid A (250F) Liquid B – 121°C Waste* (250F) Liquid A – 121°C √ Cooling* (250F) Liquid B 121°C √...
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Sterilization Programs Temp. models models models only only only Icon Description Bio Hazard √ Liquids* (250F) √ Vacuum test (PV only)* 80 °C Warm-Up* 176F) 80 °C Isothermal* 176F) 121C Mixture* (250F) 121C Glass Test* (250F) 121C Durham* (250F) *These programs are optional Page 30...
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During the process, the stages of the cycle will be displayed on the screen: The stages names are as follows: Start Pulse Low (PV models only) Pulse High (PV models only) Water Inlet Heating Sterilization Cooling (C models only) Exhaust Drying (PV model only) Ending Page 31...
5.2 Program 2: Plastic For plastic and other delicate instruments, when the manufacturer recommends autoclaving at temperatures of 121C (250F). Drying stage is available for PV (pre-vacuum) models only. Nominal parameters default settings • Sterilization temperature: 121C (250F) • Sterilization time: 15 minutes •...
5.3 Program 3: Liquid A For liquids when the manufacturer recommends autoclaving at temperatures of 121C (250F) for 15 minutes. Cautions! Both PT100 temperature sensors must be inside the bottles. For proper sterilization, fill the bottles with approximately the same amount of liquid. Nominal parameters default settings •...
Then steam is exhausted out of the chamber at a fast rate until pressure decreases to ambient pressure. • Drying: N/A • Ending: The system checks that End temperature and End pressure have been reached, and then releases the door. 5.4 Program 4: Liquid B –...
• Cooling: N/A • Slow Exhaust: Steam is exhausted from the chamber at a slow rate, until it reaches the pressure of 30 kPa above the ambient pressure. Then steam is exhausted out of the chamber at a fast rate until pressure decreases to ambient pressure.
• Heating: The chamber is heated by actuation of electrical heaters until the sterilization temperature is reached. • Sterilization: Sterilization temperature is maintained constant during the sterilization time. • Cooling: Forced cooling to the required end temperature, chamber pressure remains at approx. 300 kPa and the cooling valve is opened.
Operations sequence: • (PV, pre-vacuum models only) Pulse low/Pulse high: at one pulse it will build vacuum down to 25 kPa. • Water Inlet: Water enters the chamber • Heating: The chamber is heated by actuation of electrical heaters until the sterilization temperature is reached. •...
• Drying time: 1 minute. (PV – pre-vacuum models only). Operations sequence: • (PV, pre-vacuum models only) Pulse low/Pulse high: at one pulse it will build vacuum down to 25 kPa. All exhaust from the chamber is performed through the bio-hazard filter. •...
Nominal parameters default settings • Sterilization temperature: 121C (250F) • Sterilization time: 45 minutes • Drying time: 1 minute. (PV – pre-vacuum models only). Operations sequence: • (PV, pre-vacuum models only) Pulse low/Pulse high: at one pulse it will build vacuum down to 25 kPa. All exhaust from the chamber is performed through the bio-hazard filter.
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All exhaust from the chamber before completion of the Sterilization stage is performed through the bio-hazard filter. For liquids when the manufacturer recommends autoclaving at temperatures of 121ºC (250F) for 45 minutes. Cautions! Both PT100 temperature sensors must be inside the bottles. For proper sterilization, fill the bottles with approximately the same amount of liquid.
5.10 Program 10: Vacuum Test (PV, pre-vacuum models only) Vacuum Test is a test program with the following parameters: Nominal parameters default settings • Vacuum pressure: 17.0 kPa • Vacuum time 1: 5 minutes • Vacuum time 2: 10 minutes Operations sequence: •...
5.12 Program 12: Isothermal For liquids when the manufacturer recommends autoclaving at 60- 100C (140-212F) with no drying. Recommending for melting of agar, pasteurization etc. Caution! This is not a sterilization program! Put one PT100 inside the bottle, leave the second one hanging in the chamber outside the bottle (see below).
• Heating: The chamber is warmed up until the Keep-Heat temperature is reached inside the chamber. • Keep Heat: Keep-Heat temperature is maintained constant for the preset Keep-Heat time. • Cooling: N/A. • Fast Exhaust: the steam is exhausted out of the chamber at a fast rate until pressure decreases to ambient pressure.
• Sterilization temperature: 121C (250F). • Sterilization time: 15 minutes. Operations sequence: • Heating: Air enters the chamber followed by steam that heats it up until the sterilization temperature is reached. Air adds pressure needed to prevent swelling or warping of soft plastic items. Chamber fan is used to mix air with steam.
Nominal parameters default settings • Sterilization temperature: 121C (250F). • Sterilization time: 20 minutes. Operations sequence: • Heating: Steam enters the chamber and heats it up until the sterilization temperature is reached. During the interval of the heating stage, between 100°C and 121°C, the heating rate is kept to approximately a rise of 1 degree per minute.
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For liquids when the manufacturer recommends autoclaving at temperatures of 121ºC (250F) for 15 minutes. Cautions! Both PT100 temperature sensors must be inside the bottles. For proper sterilization, fill the bottles with approximately the same amount of liquid. Nominal parameters default settings •...
6. Checking and Changing Parameters and Other Data Bacsoft control panel allows changing parameters of the cycle and of the system, exporting various data to, and importing from, a USB device or to the printer, and some other options. Cycle parameters are changeable for Custom programs only (see Duplicate cycles), with the exception of the Temperature sensors, Displayed inputs, and Dry Time.
Note: To exit every screen and to return to the previous screen (to get one level up): • move the cursor to Exit by pressing the UP or DOWN keys and then press the Start/Stop key. - or- • press the UP and DOWN keys simultaneously. In the next chapter you will see how to change the required parameter as desired.
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Note: Please note the maximum and minimum values for this parameter shown on the screen. Your value must be within these boundaries. Below is the example of changing the Dry time parameter on the screen used in the previous section: Page 51...
Note: To exit every screen and to return to the previous screen: • move the cursor to Exit by pressing the UP or DOWN keys and then press the Start/Stop key - or- • press the UP and DOWN keys simultaneously 6.3 Quick options screen When the autoclave is on and no cycle is running, press Up and Down keys simultaneously to enter the Quick options screen.
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Below you can find instructions how to login and enter the Main menu. Section 7.1 above explains how to browse through the menus; section 7.2 explains how to change a parameter. Below is the explanation of some Quick Options. 6.3.1 Version Information This directory allows viewing information of the current, factory default, and previous software versions.
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On the Start cycle by clock screen, the time is displayed in the form “HH:MM”. The hour range is 24 hours (i.e. from "0" to "24"). Setting the time to start the cycle Move the cursor to the Time field. Set the required time .
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Disabling the START CYCLE BY CLOCK On the Start Cycle by Clock screen, move the cursor to Disabled. Press Up or Down key to disable Starting cycle by clock. Exit the Enabling the Start Cycle by Clock. 6.3.3 Set date and time This subdirectory enables the operator to set date and time.
Caution! After setting time and date, turn the autoclave off and then on again. 6.4 Logging in and entering the Main menu Below you can find instructions how to login and enter the Main menu. Section 7.1 above explains how to browse through the menus, section 7.2 explains how to change a parameter.
Choose User, then press the Start/Stop key to enter. The following screen will appear: 0000 is displayed on the screen with the cursor flashing on the right digit. • Set the code to 0001. You will get to the Main menu. Below is the list and the explanation of some options available to user on the Main Menu.
Below is the typical parameter changing screen: 6.6 System Parameters This menu is listing the system parameters that are the same for all cycles. Browse to the following folder: Main menu\System parameters You will see the following screen: 6.6.1 Screen Saver In this menu you can define the screensaver delay time, i.
6.7 Maintenance Maintenance procedures provided by Bacsoft software allow you additional tests and USB input/output options. Browse to the following folder: Main menu\Maintenance You will see the following screen listing the maintenance options: Below is the instruction for autoclave’s maintenance menu. 6.7.1 Reset atmospheric pressure In this menu you can reset the atmospheric pressure value.
Note: Please reset the atmospheric pressure when you install the autoclave for the first time, and each time you relocate or calibrate the autoclave. 6.7.2 Printer test In this menu you can check the normal function of the printer. The printer will print the list of errors.
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Note: For all the standard sterilization cycles, and for Bowie and Dick test, the only changeable cycle parameter is dry time (you will not see other parameters on your screen). For the custom cycles created by duplication, and for the Warm Up cycle, more options are changeable. Operator is not allowed to create custom cycles;...
7. Screens 7.1 Screens following a complete successfully cycle – "Cycle Ended" 1. System Ready 2. Pulse Low (ELPV 3. Pulse High (1/1) model only) (ELPV model only) 4. Heating 5. Sterilization 6. Exhaust 7. Drying (ELPV 8. Ending 9. Cycle Ended model only) Display can be activated only by an authorized person.
7.2 Screens following aborted cycles after complete sterilization stage The sterilization phase ended successfully – cycle ended and the reason of failure is displayed For example the next two scenarios: 7.2.1 Canceled by user after complete sterilization stage The cycle ended successfully, the reason for aborted cycle is displayed.
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7.3.2 Failure according to Cancellation by user before complete sterilization stage When "Cycle Failed" appears on the screen, the user shall press START/STOP key in order to delete the "Cycle Failed" message An example for all displayed warnings according to Cycle Failed: Cycle Failed message Reason of failure The screen colour changed...
8. Printer 8.1 Printer Output The printing is on thermal paper with 24 characters per line and contains the following information: • Date: • Time: • Ser. Num: • Model: • Version: • Cycle Num: • Cycle: • Dry Time: •...
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The time, temperature and pressure during E 00:22:08 134.5 311.9 exhaust CLK 2: 12:12:10:00 CLK 1: 12:12:10:00 The time, temperature and pressure during S 00:22:07 134.5 311.6 sterilization The time, temperature and pressure during S 00:22:06 134.5 311.6 sterilization The time, temperature and pressure during S 00:21:06 134.6 311.0...
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TIME °C Ster Time: 4.0min Sterilization time for selected program Sterilization temperature in chamber for selected Ster Temp: 134.0°C program Unwrapped Cycle: Cycle name instru Cycle Num: 000001 Cycle number Software version A.B.CC.DD = 7.0.00.00 A: Door Type: Single motor = 7 B: Vacuum Type = 0 Version: 7.0.00.00...
Heating stage Exhaust stage Keep Heat (Optional) Drying Stage (ELPV Model only) 8.2 Printer Handling 8.2.1 Maintenance Wipe off the soiling on the printer surface with a dry soft cloth with a weak neutral detergent. After that, wipe the printer with a dry cloth. 8.2.2 Setting paper PLUS II Front view 1-Paper mouth...
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Fig. 2 Press the OPEN key to open the printer cover as shown (see Fig. 3/1). Handle the paper cutter carefully not to cut your hand. Place the paper roll making sure it unrolls in the proper direction as shown (see Fig. 3/2). Take out the paper and re-close the cover as shown (see Fig.
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8.2.3 Treating the thermal papers: • Store the papers in a dry, cool and dark place. • Do not rub the papers with hard substance. • Keep the papers away from organic solvent. Cautions Never disassemble the printer. Failure to follow this instruction may cause overheating or burning of the printer or the AC adapter.
9. Preparation before Sterilization The purpose of packaging and wrapping of items for sterilization is to provide an effective barrier against sources of potential contamination in order to maintain sterility and to permit aseptic removal of the contents of the pack. Packaging and wrapping materials should permit the removal of air from the pack, penetration of the sterilizing water vapor into the pack and removal of the sterilizing vapor.
Disassemble or sufficiently loosen multiple-part instruments prior to packaging to permit the sterilizing agent to come into contact with all parts of the instrument. Tilt on edge items prone to entrap air and moisture, e.g. hollowware, so that only minimal resistance to removal of air, the passage of steam and condensate will be met.
10. Operating Instructions 10.1 Draining and filling the water reservoir (WR only). Draining CAUTION! Before starting, Make sure that the electric cord is disconnected and there is no pressure in the autoclave. The drain valve is located on the bottom of the water reservoir (See 3.14 .
Cautions! Use only water having the characteristics as per table in sec 4. Tap water may clog the system. A clogged system causes increase of pressure, which prevent temperature from rising. Under no circumstance should water be filled above the cooling coil.
10.4 Loading • Load the autoclave properly according to instructions in sec. 9. PREPARATION BEFORE STERILIZATION. • Select the program. ▪ UP key: next program. ▪ DOWN key: previous program. • Verify that you chose the required program. 10.5 Closing the door Hold the opening handle in open position, while pushing the door until it comes to closed position, then release the handle.
• Wear heat-resistant gloves or use the tray handle to remove the load from the autoclave • On completion of the cycle, the load shall be visual inspected to ascertain that the load is dry, and that the colour of the sterilization indicators turned to the required colour.
Caution: Before moving the autoclave, verify that the electrical, air and water connections have been disconnected, and there is no pressure in the chamber. Do not drop this device! 10.11 Loading and Unloading the Device 10.11.1 Safety Protective equipment and clothes and other safety instructions should be implemented in accordance with local and national regulations and/or rules! For proper sterilization - Do not overload the chamber.
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To avoid injuries use heat resistant gloves while unloading the autoclave. Page 79...
11. Service and Maintenance 11.1 Preventive Maintenance The maintenance operations described in this chapter must be fulfilled periodically in order to keep the autoclave in good working condition and to reduce the breakdown time to a minimum. The user’s maintenance personnel, according to the following instructions can easily execute these operations.
Put a few drops of oil on the two door pins and door tightening bolts. Clean outer parts of the autoclave with a soft cloth. 11.1.3 Periodically Every 6 months replace the air filter, (if installed) (to be done by an authorized technician).
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Pressure-Regulating Nut Rear Wall 11.2.2 ASME-approved type safety valve Operate the sterilization cycle according to the manual. Allow a pressure of approximately 200 kPa (29-psi) to build up in the chamber. Operate the safety valve by pulling the ring of the safety valve using a tool, i.e.
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Attention: Use protective gloves in order not to burn your hands with the hot steam. Safety valve ring Page 83...
12. Troubleshooting This troubleshooting chart enables the user to solve minor malfunctions, prior to contacting our service department. Only technical personnel having proper qualifications and holding technical documentation (including a technician manual) and adequate information are authorized to service the apparatus.
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Message / Symbol / Failure Description Corrective Action Problem the temperature drops for more than 1 second below the sterilization temperature during sterilization cycle. This message is displayed if the temperature raises 7°F (4°C) above sterilization High Temp temperature during the Perform a new cycle.
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Message / Symbol / Failure Description Corrective Action Problem seconds during the sterilization stage. This message is displayed if High the system cannot reach Pressure Perform a new cycle. atmospheric pressure ± 10kPa (Ending) during the ending stage. This message is displayed if High the system cannot reach Pressure...
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Message / Symbol / Failure Description Corrective Action Problem check time time has passed. exceeded - Please call for service Cycle Number of cycles, since last counter periodical maintenance, exceeded - exceeded the preset number Call for service. Please call as defined by "cycle counter"...
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Message / Symbol / Failure Description Corrective Action Problem This message is displayed if Chamber the pressure in the chamber is pressure Call for service. too high or too low from the not in range normal range. This message is displayed if I/O Card I/O card is faulty (both while Call for service.
13. Baskets and Containers Container for waste products High Basket Low Basket Stainless steel container for Stainless steel wire baskets waste products, with vent holes Dia. x Height (mm) Capacity Dia. x Height (mm) Capacity 250 x 190 265 x 200 250 x 250 265 x 390 14.
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