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Operation and Maintenance Manual Electronic Laboratory Autoclaves Models LABSCI 11LV This device is not a medical device and not intended for medical use. LV-D: standard autoclave C: optional system for fast cooling PV: optional vacuum pump BH: optional bio hazard filtration...
Table of Contents Paragraph page no. 1. GENERAL INFORMATION Available optional configurations ............... 5 Incoming Inspection ..................6 Warranty ...................... 6 Warranty Statement ..................7 Safety Instructions ....................8 Technical Data ....................10 Operating Conditions ................. 10 Directives and Standards ................11 Ordering Information ................
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Program 7: Bio Hazard 1 (BH, bio-hazard models only) ......41 Program 8: Bio Hazard 2 (BH, bio-hazard models only) ......42 Program 9: Bio Hazard Liquids (BH, bio-hazard models only) ....43 5.10 Program 10: Vacuum Test (PV, pre-vacuum models only) ...... 44 5.11 Program 11: Warm-Up ................
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10.11 Loading and Unloading the Device ............84 Service and Maintenance ................... 86 11.1 Preventive Maintenance ................86 11.2 Checking the Safety Valve ................. 87 Troubleshooting ....................90 Baskets and Containers ..................95 Spare Parts List ....................95 Accessories ......................96 Page 3...
This device is not a medical device and not intended for medical use. LABSCI 11LV is a vertical sterilizer designed especially for sterilization of instruments, liquids and other materials in hospital laboratories, laboratories & research institutes, food laboratories and pharmaceutical facilities.
After reading this manual, operating the autoclave should be straight forward. However, since the autoclave is built using high technology sensitive components, no attempt should be made by the user or any other unauthorized person to repair or re-calibrate it. Only technical personnel having proper qualifications, holding technical documentation and adequate test instrumentation, are authorized to undertake repair or service.
All Tuttnauer products are carefully inspected prior to shipment and all reasonable precautions are taken in preparing them for shipment to assure safe arrival at their destination.
Troubleshooting, Service incidents, Warranty repair, Repair, Services and Return authorization : 224-265-9600 Fax: 224-265-9611 e-mail: service@heidolph.com Tuttnauer USA Co., Ltd., 25 Power Drive Hauppauge, NY 11788, USA : (800) 624 5836, (631) 737 4850, Fax: (631) 737 0720 e-mail:info@tuttnauerUSA.com. Note: If there is any difficulty with this instrument, and the solution is not covered in this manual, contact our representative or us first.
2. Safety Instructions The autoclave has unique characteristics. Please read and understand the operation instructions before first operation of the autoclave. The following issues may require instructions guidance provided by the manufacturer: how to operate the autoclave, the door safety mechanism, the dangers involved in circumventing safety means, how to ensure that the door is closed, and how to select a correct sterilization program.
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Individual glass bottles may be placed within an appropriate container that will be placed in a basket. Never place glass bottles on the floor of the autoclave. Never fill more than 2/3 of the bottle volume. On closing the autoclave's door, make sure it is properly locked before activating.
3. Technical Data 3.1 Operating Conditions The autoclave is intended to work in ‘indoor’ conditions only. Only autoclavable materials shall be used. The environment shall not exceed an ambient temperature range of 5ºC (41ºF)-40ºC (104ºF) and a relative humidity of 85% respectively. ...
3.2 Directives and Standards Every autoclave meets the provisions of the following Directives and is in compliance with the following Standards: ISO 9001: Quality Management System ISO 14001: Environmental Management System ISO 17025: General requirements for the competence of testing and calibration autoclaves ASME Code Section I and section VIII.
The baskets are made of stainless-steel wire and have a handle. The basket allows the operator to load a large quantity of materials into the chamber. • Stainless steel containers - Different size containers are available for these units. The containers are designed for sterilizing waste material. The containers have vent holes along the upper rim.
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Rest of heavy metals ≤ 0.1 mg/l ≤ 0.1 mg/l except iron, cadmium, lead ≤ 2 mg/l ≤ 0.1 mg/l Chloride (Cl) ≤ 0.5 mg/l ≤ 0.1 mg/l Phosphate Conductivity (at ≤ 3 µs/cm 15 µs/cm 20°C) pH value 5 to 7.5 5 to 7 ≤...
3.9 Construction Chamber and door material Stainless steel Outer Cabinet Stainless Steel Chamber insulation Fiberglass with reinforced material 3.10 Utilities Power 1 Ph, 230V/50/60Hz Power Supply Recommended Circuit Breaker Compressed Air (LVC only) 1/2" 3 Bar (44-58 psi) Tap water 1/2", 2-6 Bar (29-44 psi) Mineral free water 1/2", 2-3 Bar (29-44 psi)
3.12 Front View Description Description Door handle Printer Door cover USB socket Right service door Display Right service door grip Keyboard Front leg Main switch Front service cover Printer cover Ventilation grill Page 17...
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3.12.1 Front View: What is Special for WR Configuration Description Description Glass tube indicator of the Inlet for manual water reservoir water level reservoir filling Wheel Page 19...
3.13 Rear View No. Description No. Description Air pressure regulator (C – cooling models Fan grill only) Air pressure gauge (C – cooling models only) Left service door grill Compressed air inlet (C – cooling models Left service door lock only) Mineral free water inlet RJ45 connector...
3.15 Overall Dimensions, WR Configuration(mm) 3.16 Safety Features This autoclave includes built-in safety features such as: Error message display. Electronic pressure and temperature measurement. Safety relief valve to avoid build-up of excessive pressure. Door switches enabling operation to be started only when the door is closed.
Excess temperature protection. 3.17 Description of Operation 3.17.1 Heat The vertical autoclaves are equipped with immersion type, heating elements. After water has been introduced to the chamber and the unit has been activated, the heating elements begin to heat. The temperature and pressure in the chamber increase until appropriate levels are reached.
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flow is stopped automatically, process is completed, and it is possible to open the door and take out the sterilized goods from the chamber. 3.17.4 Exhaust When the timed sterilization cycle is complete, the unit enters the exhaust stage, provided that a program other than the liquid program was selected.
4.2 Description and Functions of the Front Panel Keyboard The front panel is composed of 3 sections: Display screen. Keypad. Printer 4.2.1 Display screen The display is an LCD panel used to display the current status of the autoclave while using Operational Messages and Error Messages. 4.2.2 Keypad The keypad consists of three keys as described below: UP key...
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increases its value. When the cursor is blinking on a menu selection, the UP ▲ key allows browsing backward through the menu. When adjusting a parameter and the cursor is blinking on “SET” or “EXIT” the UP ▲ key activates that procedure.”...
4.3 Displayed Error Messages / Symbols The failures are divided into two categories . Failure that occur before completing the sterilization stage, which in this case will leave the load unsterilized Failure that occur after completing the sterilization stage, which in this case will leave the load sterilized For the list of Displayed Error Messages / Symbols see sec.
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Critical settings have been If a change of the autoclave updated, please Restart the autoclave for setting was made, a restart restart machine changes to be updated. operation is required. for changes to be updated This message is displayed if Perform a new cycle to the electrode in the chamber drain the chamber.
5.1 Program 1: Glass For glass instruments when the manufacturer recommends autoclaving at temperatures of 134C (273F). Drying stage is available for PV (pre-vacuum) models only. Nominal parameters default settings • Sterilization temperature: 134C (273F). • Sterilization time: 3 minutes. •...
5.2 Program 2: Plastic For plastic and other delicate instruments when the manufacturer recommends autoclaving at temperatures of 121C (250F). Drying stage is available for PV (pre-vacuum) models only. Nominal parameters default settings • Sterilization temperature: 121C (250F) • Sterilization time: 15 minutes •...
5.3 Program 3: Liquid A For liquids when the manufacturer recommends autoclaving at temperatures of 121C (250F) for 15 minutes. Cautions! Both PT100 temperature sensors must be inside the bottles. For proper sterilization, fill the bottles with approximately the same amount of liquid. Nominal parameters default settings •...
• Ending: The system checks that End temperature and End pressure have been reached, and then releases the door. 5.4 Program 4: Liquid B – Waste For liquids when the manufacturer recommends autoclaving at temperatures of 121C (250F) for 30 minutes, such as liquid waste. Cautions! Both PT100 temperature sensors must be inside the bottles.
Then steam is exhausted out of the chamber at a fast rate until pressure decreases to ambient pressure. • Drying: N/A • Ending: The system checks that End temperature and End pressure have been reached, and then releases the door. 5.5 Program 5: Liquid A –...
• Cooling: Forced cooling to the required end temperature, chamber pressure remains at approx. 300 kPa and the cooling valve is opened. • Fast Exhaust: The steam is exhausted out of the chamber at a fast rate until pressure decreases to ambient pressure. •...
• Heating: The chamber is heated by actuation of electrical heaters until the sterilization temperature is reached. • Sterilization: Sterilization temperature is maintained constant during the sterilization time. • Cooling: Forced cooling to the required end temperature, chamber pressure remains at approx. 300 kPa and the cooling valve is opened.
• (PV, pre-vacuum models only) Pulse low/Pulse high: At one pulse it will build vacuum down to 25 kPa. All exhaust from the chamber is performed through the bio-hazard filter. • Water Inlet: Water enters the chamber • Heating: The chamber is heated by actuation of electrical heaters until the sterilization temperature is reached.
• Sterilization time: 45 minutes • Drying time: 1 minute. (PV – pre-vacuum models only). Operations sequence: • (PV, pre-vacuum models only) Pulse low/Pulse high: At one pulse it will build vacuum down to 25 kPa. All exhaust from the chamber is performed through the bio-hazard filter.
Cautions! Both PT100 temperature sensors must be inside the bottles. For proper sterilization, fill the bottles with approximately the same amount of liquid. Nominal parameters default settings • Sterilization temperature: 121C (250F) • Sterilization time: 45 minutes Operations sequence: • (PV, pre-vacuum models only) Pulse low/Pulse high: At one pulse it will build vacuum down to 25 kPa.
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only) Vacuum Test is a test program with the following parameters: Nominal parameters default settings • Vacuum pressure: 17.0 kPa • Vacuum time 1: 5 minutes • Vacuum time 2: 10 minutes Operations sequence: • Vacuum is produced in the chamber down to P1=17 kPa. At this stage all the valves close.
5.12 Program 12: Isothermal For liquids when the manufacturer recommends autoclaving at 60- 100C (140-212F) with no drying. Recommending for melting of agar, pasteurization etc. Caution! This is not a sterilization program! Put one PT100 inside the bottle, leave the second one hanging in the chamber outside the bottle (see below).
• Heating: The chamber is warmed up until the Keep-Heat temperature is reached inside the chamber. • Keep Heat: Keep-Heat temperature is maintained constant for the preset Keep-Heat time. • Cooling: N/A. • Fast Exhaust: The steam is exhausted out of the chamber at a fast rate until pressure decreases to ambient pressure.
• Sterilization time: 15 minutes. Operations sequence: • Heating: Air enters the chamber followed by steam that heats it up until the sterilization temperature is reached. Air adds pressure needed to prevent swelling or warping of soft plastic items. Chamber fan is used to mix air with steam.
• Sterilization temperature: 121C (250F). • Sterilization time: 20 minutes. Operations sequence: • Heating: Steam enters the chamber and heats it up until the sterilization temperature is reached. During the interval of the heating stage, between 100°C and 121°C, the heating rate is kept to approximately a rise of 1 degree per minute.
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Cautions! Both PT100 temperature sensors must be inside the bottles. For proper sterilization, fill the bottles with approximately the same amount of liquid. Nominal parameters default settings • Sterilization temperature: 121C (250F). • Sterilization time: 15 minutes. Operations sequence: • (PV, pre-vacuum models only) Pulse low/Pulse high: At one pulse it will build vacuum down to 25 kPa.
6. Checking and Changing Parameters and Other Data Bacsoft control panel allows changing parameters of the cycle and of the system, exporting various data to, and importing from, a USB device or to the printer, and some other options. Cycle parameters are changeable for Custom programs only (see Duplicate cycles), except for the Temperature sensors, Displayed inputs, and Dry Time.
Note: To exit every screen and to return to the previous screen (to get one level up): • Move the cursor to Exit by pressing the UP or DOWN keys and then press the Start/Stop key. - or- • Press the UP and DOWN keys simultaneously. In the next chapter you will see how to change the required parameter as desired.
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Note: Please note the maximum and minimum values for this parameter shown on the screen. Your value must be within these boundaries. Below is the example of changing the Dry time parameter on the screen used in the previous section: Page 54...
Note: To exit every screen and to return to the previous screen: • Move the cursor to Exit by pressing the UP or DOWN keys and then press the Start/Stop key - or- • Press the UP and DOWN keys simultaneously 6.3 Quick options screen When the autoclave is on and no cycle is running, press Up and Down keys simultaneously to enter the Quick options screen.
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Below you can find instructions how to login and enter the Main menu. Section 6.1 above explains how to browse through the menus; section 6.2 explains how to change a parameter. Below is the explanation of some Quick Options. 6.3.1 Version Information This directory allows viewing information of the current, factory default, and previous software versions.
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Enter the Start cycle by clock screen. The following screen will appear: On the Start cycle by clock screen, the time is displayed in the form “HH:MM”. The hour range is 24 hours (i.e. from "0" to "24"). Setting the time to start the cycle Move the cursor to the Time field.
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Disabling the START CYCLE BY CLOCK On the Start Cycle by Clock screen, move the cursor to Disabled. Press Up or Down key to disable Starting cycle by clock. Exit the Enabling the Start Cycle by Clock. 6.3.3 Set date and time This subdirectory enables the operator to set date and time.
Caution! After setting time and date, turn the autoclave off and then on again. 6.4 Logging in and entering the Main menu Below you can find instructions how to login and enter the Main menu. Section 6.1 above explains how to browse through the menus, section 6.2 explains how to change a parameter.
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Choose User, then press the Start/Stop key to enter. The following screen will appear: 0000 is displayed on the screen with the cursor flashing on the right digit. • Set the code to 0001. You will get to the Main menu. Page 60...
Below is the list and the explanation of some options available to user on the Main Menu. 6.5 Changing parameters Bacsoft control panel provides an interface that consists of control screens available through an easy scrollable menu tree. To learn how to scroll through the menus, change the parameters, and perform some other functions using our three-button keypad, see 6.1 and 6.2.
6.6 System Parameters This menu is listing the system parameters that are the same for all cycles. Browse to the following folder: Main menu\System parameters You will see the following screen: 6.6.1 Screen Saver In this menu you can define the screensaver delay time, i. e. how long the keyboard will be untouched before the screensaver activates.
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Below is the instruction for autoclave’s maintenance menu. 6.7.1 Reset atmospheric pressure In this menu you can reset the atmospheric pressure value. To do so: Browse to the following folder: Maintenance\Reset atmospheric pressure The following screen will appear: Leave the door open for 2 minutes at least. Ambient temperature should be less than 45°C.
The following screen will appear to confirm that the test has been done. See the printout shown in the Printer handling chapter of this manual. 6.8 Cycle parameters The Cycle parameters menu includes parameters of a specific sterilization program (cycle). Browse to the following folder: Main menu\Cycle parameters You will see the following screen listing the cycle parameters: Note: For all the standard sterilization cycles, and for Bowie and Dick test, the...
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6.8.1 Dry Time This parameter defines duration of the drying stage for the cycle. Browse to the following folder: Cycle parameters\Drying\Dry Time Change the parameter as desired. Drying stage is divided into two stages. For each stage you can set the total time, on time and off time.
7.2 Screens following aborted cycles after complete sterilization stage The sterilization phase ended successfully – cycle ended, and the reason of failure is displayed For example, the next two scenarios: 7.2.1 Canceled by user after complete sterilization stage The cycle ended successfully, the reason for aborted cycle is displayed.
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7.3.2 Failure according to Cancellation by user before complete sterilization stage When "Cycle Failed" appears on the screen, the user shall press START/STOP key to delete the "Cycle Failed" message An example for all displayed warnings according to Cycle Failed: Cycle Failed message Reason of failure The screen color changed...
8. Printer 8.1 Printer Output The printing is on thermal paper with 24 characters per line and contains the following information: • Date: • Time: • Ser. Num: • Model: • Version: • Cycle Num: • Cycle: • Dry Time: •...
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exhaust The time, temperature, and pressure during E 00:22:08 134.5 311.9 exhaust CLK 2: 12:12:10:00 CLK 1: 12:12:10:00 The time, temperature, and pressure during S 00:22:07 134.5 311.6 sterilization The time, temperature, and pressure during S 00:22:06 134.5 311.6 sterilization The time, temperature, and pressure during S 00:21:06 134.6...
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7.0.00.00 C: Total number of Input/Output functionality that are not as default = 00 D: Total number of parameters values that are not as default = 00 Model: LABSCI 11LV Model name Ser. Num: 000000000001 Model Serial number Time: 11:50:05...
Legend Insert Water Sterilization stage Steam Flush CLK 1 Real Time Clock Air removal stage CLK 2 Software clock Heating stage Exhaust stage Keep Heat (Optional) Drying Stage (LPV Model only) 8.2 Printer Handling 8.2.1 Maintenance Wipe off the soiling on the printer surface with a dry soft cloth with a weak neutral detergent.
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Fig. 1 Open the printer's cover door (1) by pulling it up (see Fig. 2). Page 73...
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Fig. 2 Press the OPEN key to open the printer cover as shown (see Fig. 3/1). Handle the paper cutter carefully not to cut your hand. Place the paper roll making sure it unrolls in the proper direction as shown (see Fig. 3/2). Take out the paper and re-close the cover as shown (see Fig.
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8.2.3 Treating the thermal papers: • Store the papers in a dry, cool, and dark place. • Do not rub the papers with hard substance. • Keep the papers away from organic solvent. Cautions Never disassemble the printer. Failure to follow this instruction may cause overheating or burning of the printer or the AC adapter.
9. Preparation before Sterilization The purpose of packaging and wrapping of items for sterilization is to provide an effective barrier against sources of potential contamination to maintain sterility and to permit aseptic removal of the contents of the pack. Packaging and wrapping materials should permit the removal of air from the pack, penetration of the sterilizing water vapor into the pack and removal of the sterilizing vapor.
Disassemble or sufficiently loosen multiple-part instruments prior to packaging to permit the sterilizing agent to come into contact with all parts of the instrument. Tilt on edge items prone to entrap air and moisture, e.g. hollowware, so that only minimal resistance to removal of air, the passage of steam and condensate will be met.
10. Operating Instructions 10.1 Draining and filling the water reservoir (WR only). Draining CAUTION! Before starting, make sure that the electric cord is disconnected and there is no pressure in the autoclave. The drain valve is located on the bottom of the water reservoir (See 3.14 .
Cautions! Use only water having the characteristics as per table in sec 3.7. Tap water may clog the system. A clogged system causes increase of pressure, which prevent temperature from rising. Under no circumstance should water be filled above the cooling coil.
The door is equipped with an electrical cylinder. This electrical cylinder performs the automatic opening and closing of the door. 10.3 Opening the door 1. Place your thumb on the plastic door cover and the other fingers in the handle. 2.
10.7 Unloading When the cycle ended successfully (including pressing START/STOP key, or any failure, after completing the sterilization stage) message "Cycle Ended" (and the relevant failure message, if applicable) is displayed on the screen. • Verify that there is no pressure in the chamber, according to the reading on the display.
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The load has not completed a sterilization cycle; therefore, it is not sterile. Handle it as contaminated load. Page 83...
10.9 Start Cycle by Clock mode • This mode enables the operator to define the time of the beginning of the cycle. The maximum possible delay is 24 hours. For further information see sec. 6.3.2. “Start Cycle by Clock”. 10.10 Moving the Autoclave Disconnect the power supply cord.
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For detailed loading instructions, see sec. 9 (Preparation before sterilization) 10.11.3 Unloading On completion of the cycle, take out the load immediately from the sterilizer. Do not remove the load from the basket until its temperature reduces to the room temperature. Let the load cool down in an area without air movement (air conditioning, etc.) and with minimum people passing by to avoid possibility of touching the hot load.
11. Service and Maintenance 11.1 Preventive Maintenance The maintenance operations described in this chapter must be fulfilled periodically to keep the autoclave in good working condition and to reduce the breakdown time to a minimum. The user’s maintenance personnel, according to the following instructions can easily execute these operations.
add water to the reservoir until the message disappears. Do not fill the reservoir completely! Put a few drops of oil on the two door pins and door tightening bolts. Clean outer parts of the autoclave with a soft cloth. 11.1.3 Periodically Every 6 months replace the air filter, (if installed) (to be done by an authorized technician).
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Use protective gloves in order not to burn your hands with the hot steam. Pressure-Regulating Nut Rear Wall 11.2.2 ASME-approved type safety valve Operate the sterilization cycle according to the manual. Allow a pressure of approximately 200 kPa (29-psi) to build up in the chamber.
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Attention: Use protective gloves in order not to burn your hands with the hot steam. Safety valve ring Page 89...
12. Troubleshooting This troubleshooting chart enables the user to solve minor malfunctions, prior to contacting our service department. Only technical personnel having proper qualifications and holding technical documentation (including a technician manual) and adequate information are authorized to service the apparatus.
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Message / Symbol / Failure Description Corrective Action Problem damaged. This message is displayed if the temperature drops for Low Temp more than 1 second below Perform a new cycle. the sterilization temperature during sterilization cycle. This message is displayed if the temperature raises 7°F (4°C) above sterilization High Temp...
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Message / Symbol / Failure Description Corrective Action Problem stage. This message is displayed if Chamber Pressure raises 4.2 psi-29 kPa above sterilization High pressure (134°C = 304 kPa, Perform a new cycle. Pressure 121°C = 205 kPa) for 2 seconds during the sterilization stage.
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Message / Symbol / Failure Description Corrective Action Problem This message is displayed at Wait until the autoclave the end of the cycle If the reaches the atmospheric Air Error autoclave does not reach the pressure and perform a atmospheric pressure after new cycle.
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Message / Symbol / Failure Description Corrective Action Problem sensor or Pressure sensor is disconnected or out of range. This message is displayed if Chamber the temperature in the temperature Call for service. chamber is too high or too not in range low from the normal range.
13. Baskets and Containers Container for waste products High Basket Low Basket Stainless steel container for Stainless steel wire baskets waste products, with vent holes Dia. x Height (mm) Capacity Dia. x Height (mm) Capacity 250 x 190 265 x 200 250 x 250 265 x 390 14.
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