Related Manuals for Thermo Scientific TZ-32 Zonal Rotor
Summary of Contents for Thermo Scientific TZ-32 Zonal Rotor
- Page 1 Thermo Scientific TZ-32 Zonal Rotor for the Thermo Scientific WX+ Centrifuge Instruction Manual 50146926-a • 01 / 2015...
-
Page 2: Table Of Contents
Thermo Scientific TZ-32 Zonal Rotor Table of Contents Preface ..........4 1. - Page 3 Thermo Scientific TZ-32 Zonal Rotor 5. 3. 2. Preparation for spinning the rotor ........30 5.
- Page 4 Thermo Scientific TZ-32 Zonal Rotor Table of Figures Rotor Components ............8 Seal attachment components (Type RPZ-S.
- Page 5 Thermo Scientific TZ-32 Zonal Rotor Table of Tables Rotor Accessories ............8 Seal Attachment Accessories .
-
Page 6: Preface
“wall effect”, large capacity, and capability of dynamic continuous loading, unloading and analytical operation of samples while the rotor is spinning. Because of the high performance and reliability, the TZ-32 Zonal rotor can be used in many fields of laboratory work and commercial production. 1. 1. Intended Use The rotor is used in combination with the according centrifuge as a laboratory product designed to separate components by generation of Relative Centrifugal Force (RCF). -
Page 7: Items Supplied
➄ Stopper (455897) ➅ Shaft (209280) ➆ Septa (103547) ➇ CAUTION The TZ-32 zonal rotor assembly does not include the RPZ-S seal attachments assembly. It has to be ordered seperately. See “Figure 2” on page 7. Intended Use — 7... -
Page 8: Rotor Accessories
Thermo Scientific TZ-32 Zonal Rotor Rotor Accessories Table 1 Item Article No. Note Quantity Picture Rotor Accessories Rulon seal 439345 Rotor stand 216715 Seal packing 451153 TZ-32 O-ring 8062222 1AS22 (JIS-B-2401) For rotor O-ring 8062014 1AP14 (JIS-B-2401) For Rulon seal... -
Page 9: Seal Attachment Assembly
Thermo Scientific TZ-32 Zonal Rotor 1. 2. 2. Seal Attachment Assembly Seal Attachment Components Figure 2 Seal attachment Œ components (Type RPZ-S. Part cord. 901306) Ž Part 1 of the shield plate assembly (322897B) ➀ Guard plate assembly (104230A) ➁... -
Page 10: Seal Attachment Accessories
Thermo Scientific TZ-32 Zonal Rotor Seal Attachment Accessories Table 2 Item Article No. Note Quantity Picture Seal Attachment Hexagon socket head 70017308 M6 x 30 Accessories bolt D6 spring washer 80112078 D6 flat washer 80202088 Tee connector 484286 Rubber tube clamp... -
Page 11: Precautions
Thermo Scientific TZ-32 Zonal Rotor 1. 3. Precautions • Do not spin a rotor beyond the allowable speed. » The maximum speed marked on a rotor refers to an allowable one when the maximum density of the sample or density gradient solution is less than 1.2. -
Page 12: Technical Specifications
Thermo Scientific TZ-32 Zonal Rotor Technical Specifications Table 3 Maximum Speed 32000 rpm Technical Maximum Centrifugal Force 102000 x g Specifications K-factor Capacity 1690 ml Maximum Density of Gradient Solution 1.2 g/ml Maximum Radius 8.89 cm Rotor Material Titanium Alloy Weight 15.4 kg... -
Page 13: Construction
Thermo Scientific TZ-32 Zonal Rotor Construction 3. 1. High-Speed Rotation Figure 3 Construction – Œ High Speed Rotation ➀ Cover of rotor ➁ 3. 2. Loading and Unloading Figure 4 Construction – Loading and Unloading ... -
Page 14: Assembly
Thermo Scientific TZ-32 Zonal Rotor Assembly 4. 1. Rotor Assembly The rotor is composed of a rotor body, cover, septa, shaft, cap assembly and Rulon ™ seal attached to the shaft. The TZ-32 rotor has a capacity of 1690 ml. -
Page 15: Shaft
Thermo Scientific TZ-32 Zonal Rotor 4. 1. 5. Shaft A shaft is inserted in the central part of septa, and form a sample passage way. And, the tops of the shaft become the installation of the cap and the connection part of seal assembly. The material of the shaft uses titanium alloy. -
Page 16: Rpz-S Seal Attachment Assembly
Thermo Scientific TZ-32 Zonal Rotor 4. 2. RPZ-S Seal Attachment Assembly 4. 2. 9. Seal assembly The seal assembly is mounted onto the rotor shaft as shown in Figure 10. The rotor, shaft, and adapter rotate, but the other components do not rotate. The outer side of the holder cover is supported by the guard plate which will be described later. -
Page 17: Construction Of The Seal Assembly
Thermo Scientific TZ-32 Zonal Rotor Figure 10 Construction of the seal assembly Œ Ž ‘ ’ “ ” • Body cooling water pipes ➀ Pipe in the rotor center ➁ Cooling water pipe ➂ Coil spring (B) ➃... -
Page 18: Guard Plate Assembly
Thermo Scientific TZ-32 Zonal Rotor 4. 2. 10. Guard plate assembly The center case supports the seal assembly and maintains the contact between the body and the Rulon seal for injecting solution into and extracting it, from the spinning rotor. It is mounted by using the three supports leading from the rotor chamber of the centrifuge. -
Page 19: Operation
Thermo Scientific TZ-32 Zonal Rotor Operation CA UT I O N Never spin the rotor at a speed over 5,000 rpm unless it is filled with the liquid. If this is neglected, the septa will be damaged. CA UT I O N The over-speed decal, if corroded or discolored must be replaced immediately. -
Page 20: Employed Materials
Thermo Scientific TZ-32 Zonal Rotor Maximum Speed The maximum speed of the rotor inscribed on the rotor cover surface is determined using the maximum density of gradient solution below 1.2 g/ml. If the maximum denstity of gradient solution exceeds 1.2 g/ml, decrease the maximum speed of the rotor according to... -
Page 21: Chemical Resistance Of Noryl
Thermo Scientific TZ-32 Zonal Rotor Table 5 CA U T I O N Chemical Do not use the following substances with Noryl Resistance of Noryl Benzene Fluorine Petroleum ether Carbon tetrachloride Freon Polypropylene glycol Chlorine Furfural Pyridine Chloroform Heptane Pyridine sulfate... -
Page 22: General Description
Thermo Scientific TZ-32 Zonal Rotor 5. 1. General Description The zonal rotor is employed in the following manner. Table 6 Procedure Loading Centrifugal Separation Unloading Separation Item Procedure Solution flow Speed 3,000 rpm Specified speed 3,000 rpm Procedure Inject density gradient,... -
Page 23: Preparation
Thermo Scientific TZ-32 Zonal Rotor 5. 2. Preparation 5. 2. 1. Prepare the Centrifuge Mount the supports Mount the three supports onto the fixed pieces in the rotor chamber of the centrifuge as shown in Fig.5-1. Center and adjust height of guard plate. -
Page 24: Centering The Guard Plate
Thermo Scientific TZ-32 Zonal Rotor Mounting the guard plate Put the rotor on the spinning shaft and mount the guard plate.(In TZ-32, mount it to the upper hole in the support.) The guard plate can be fixed by inserting first the end of the fixed block into the left hole on the support, erecting the right knob and then turning it to horizontal. -
Page 25: Adjusting The Base
Thermo Scientific TZ-32 Zonal Rotor Loosen the four screws in the base, and adjust until the center of the rotor shaft coincides with that of the base. Tighten the screws and fix the base. Adjust the case, while visual checking, until it is concentrical with at the center of the shaft. -
Page 26: Tightening The Three Setscrews
Thermo Scientific TZ-32 Zonal Rotor The height of the case is adjusted as follows; Loosen the three setscrews by the hex. bar wrench accessory to this rotor, and the case can be turned. Turning clockwise lowers it, while turning counterclockwise lifts it. After adjustment, tighten the three setscrews to fix the case. -
Page 27: Connect A Tube To The Seal Assembly
Thermo Scientific TZ-32 Zonal Rotor 5. 2. 2. Connect a tube to the seal assembly Tube connection For connecting the tubes, see Figure 20. The two higher ones of the four pipes led from the seal assembly are used for injection into or extraction from the rotor. -
Page 28: Confirm Test Operation
Thermo Scientific TZ-32 Zonal Rotor Cooling water Cooling water is needed for cooling the contact body, which touches the RuIon seal. Use city water as coolant in case centrifuge is to be done at normal temperature. However use cooled water when centrifuge is to be done at a temperature below 5 °C. -
Page 29: Operation
Thermo Scientific TZ-32 Zonal Rotor 5. 3. Operation 5. 3. 1. Prepare the Rotor Put the seal packing and metal ring into the rotor. Put the O-ring into the extrusion on the center bottom of the rotor. Apply a thin coat of vacuum grease to the seal packing and O-ring. -
Page 30: Setting The Seal Packing
Thermo Scientific TZ-32 Zonal Rotor Figure 22 Setting the seal packing Œ Ž Metal ring ➀ Seal packing ➁ O-Ring (1AS22) ➂ Put the Rulon seal and O-Ring into the shaft. CAUTION Be careful not to scratch the Rulon seal surface by a fingernail. When inserting the seal, carefully push it with the soft portion of finger applied. -
Page 31: Shaft Into Septa
Thermo Scientific TZ-32 Zonal Rotor Septa Figure 24 ➀ Shaft into septa Shaft ➁ Œ Put the assembled septa into the rotor. Fit the hole in the bottom end of the shaft onto the extrusion on the rotor bottom where the O-ring (1AS22) is inserted. -
Page 32: Preparation For Spinning The Rotor
Thermo Scientific TZ-32 Zonal Rotor Put the cover onto the rotor containing the septa. Put the rotor on the fixed holder base and screw in the cover by hand. Mount the handle as shown in the figure to fasten the cover. -
Page 33: Spinning
Thermo Scientific TZ-32 Zonal Rotor 5. 3. 3. Spinning CAUTION Never raise the rotor speed to more than 5000 rpm with the rotor not filled with the solution to full extent (less than 90%). High speed spinning of the rotor not full of the solution will cause the septa to be damaged. The procedure of spinning the zonal rotor depends on the type of ultracentrifuge used with the rotor. -
Page 34: Mount The Shield Plate
Thermo Scientific TZ-32 Zonal Rotor Mount the shield plate. Put the tube setter side of the shield plate to right or left end of the rotor chamber in the centrifuge. Insert the tubes from the shield plate into the tube setter through the notch of the shield plate. Mount another shield plate, and position it so that there is no clearance at the center. -
Page 35: Injection Of Sample And Overlay
Thermo Scientific TZ-32 Zonal Rotor Injection of sample and overlay Pipe on the outer wall of the rotor Clamp one end of the pipe at an outer position from the tee on the pump side, and put the other end into a beaker for receiving the “cushion”... -
Page 36: Preparation Of The Pump
Thermo Scientific TZ-32 Zonal Rotor Preparation of the pump Pipe on the outer wall of the rotor and rotor center Clamp each pipe between the tee and the seal assembly, and remove the seal assembly. Flow a heavy extracting solution through the outerwall of rotor line of the clamped pipe and eliminate bubbles from the pipe on the outer wall of rotorline. -
Page 37: High Speed Spinning
Thermo Scientific TZ-32 Zonal Rotor 5. 3. 5. High speed spinning Place the cap Hold the upper fringe as shown in the figure, and push it onto the shaft downwards. CAUTION Make sure that the cap, once set completely, will not come off the shaft. Even when it is pulled holding the fringe. -
Page 38: Deceleration And Extraction
Thermo Scientific TZ-32 Zonal Rotor gradient solution. Clamp other end. Pipe on the rotor center Clamp one end. Lead the other end to a fraction collector through a spectrophotometer flow cell. Figure 37 Deceleration and extraction Œ Œ... -
Page 39: Operational Checkpoints
Thermo Scientific TZ-32 Zonal Rotor 5. 4. Operational Checkpoints 5. 4. 1. Setting of sample temperature and rotor temperature Make the temperature of injecting sample and density gradient solution and the setting temperature of a rotor when spinning as equal as possible. If not, following problems may occur:... - Page 40 Thermo Scientific TZ-32 Zonal Rotor » When the seal assembly is removed due to an injection trouble and the connection is carried out again. a. When the rotor is full Inject a heavy solution from “OUTER WALL” side to push the bubbles out of the shaft at ”CENTER” side.
-
Page 41: Capacity In Rotor
Thermo Scientific TZ-32 Zonal Rotor 5. 5. Capacity in Rotor Figure 38 Relation between 1, 800 volume and radius 1,690 1, 600 1, 400 1, 200 1, 000 RADI US R ( cm) 5. 6. Separation Characteristic Table 9 Centrifugal Force (x g) -
Page 42: Maintenance
Thermo Scientific TZ-32 Zonal Rotor Maintenance 6. 1. Cleaning of Rotor and Septa After use, disassemble and clean the septa, shaft, metal ring, seal packing, and O-ring. Cleaning procedure: Wash it with distilled water at 40 – 50 °C after use, and wipe off water with a soft cloth. After fully drying it, coat it with stopcock grease. -
Page 43: Sterilization Of Rotor
Thermo Scientific TZ-32 Zonal Rotor 6. 2. Sterilization of Rotor Sterilize the rotor appropriately following Table 6-1. Do not autoclave the rotor or sterilize it in boiling water because its material may deteriorate and its strength may decrease. Table 11... -
Page 44: Handling Of Rulon Seal And Renewal
Thermo Scientific TZ-32 Zonal Rotor 6. 4. Handling of Rulon Seal and Renewal Rulon seal is a soft plastic with excellent lubrication developed by mixing tetrafluoroethylene resin with a special filler, and is easily scratched by a fingernail. Do not scratch the upper surface, or solution may leak due to poor sealing. -
Page 45: Disassembling And Cleaning Of Cap Assembly
Thermo Scientific TZ-32 Zonal Rotor 6. 5. Disassembling and Cleaning of Cap Assembly The cap can be disassembled by removing the snap ring using the accessory circlip remover. Be careful not to scratch it since carbon is used as a sliding agent. Wash it in water or clean it by boiling. -
Page 46: Cleaning Of Ball Bearing
Thermo Scientific TZ-32 Zonal Rotor Coil spring Figure 44 ➀ Construction of the Guide body seal assembly ➁ – Œ Body ➂ Housing ➃ — ˜ Circlip ➄ Ball bearing ➅ Ž ™ Adapter ➆ Circlip š ➇... -
Page 47: Consumable Parts Of The Rotor
Thermo Scientific TZ-32 Zonal Rotor 6. 8. Consumable Parts of the Rotor • Rulon Seal • O-Ring • Cap assembly • Seal packing • Stopper 6. 9. Service Parts List for the Cap Assembly Figure 45 Service parts list for... -
Page 48: Service Parts List For The Seal Assembly
Thermo Scientific TZ-32 Zonal Rotor 6. 10. Service Parts List for the Seal Assembly Figure 46 Service parts list for the seal assembly Item Quantity Article No. Adapter (2) 481841 60000178 Circlip 481840 Housing 437842 Ring 437844 Spring 437845 Spring holder... -
Page 49: Troubleshooting
Thermo Scientific TZ-32 Zonal Rotor Troubleshooting Table 12 Symptom Possible Cause Corrective Action Troubleshooting Pressure is 1.0 kgf/cm² or higher 1. The injection flow rate is too high. 1. The appropriate flow rate is approx. when density gradient solution is 50 ml/min. -
Page 50: Check The Rulon Seal
Thermo Scientific TZ-32 Zonal Rotor Table 13 Location Symptom Probable Cause Corrective Action Defective Part Name Portion phenomena and Bypassing and leak of Seal assembly WC sealing face (in 1. Poor sealing due to Replace the contact corrective action sample and solution. -
Page 51: Other Check Points
Thermo Scientific TZ-32 Zonal Rotor 7. 2. Other Check Points • Check of O-rings of rotor shaft for wear Remove the O-rings and check them for wear or crack by stretching a little. If worn or cracked, replace the O-rings with new ones. - Page 52 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode Germany thermoscientific.com/centrifuge © 2015 Thermo Fisher Scientific Inc. All rights reserved. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.
Need help?
Do you have a question about the TZ-32 Zonal Rotor and is the answer not in the manual?
Questions and answers