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Related Manuals for CAMAG AMD 2
Summary of Contents for CAMAG AMD 2
- Page 1 INSTRUCTION MANUAL AMD 2...
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Page 2: Table Of Contents
Further accessories ........................4 Edwards vacuum pump ........................4 Vacuum connection kit ........................5 Additional metal vacuum hose ......................5 Spare bottle set for AMD 2 ....................... 5 Unpacking/Installation ........................5 Getting started ..........................7 The AMD 2 ..........................7 Display and function of keys ...................... -
Page 3: Introduction
1 and has been designed for indoor use only (IP 20). Further, this device has passed the CAMAG Quality Assurance tests and has been delivered in safe operation condition. For detailed instrument data see chapter technical data •... - Page 4 Only authorized personnel may open the instrument. Service and repair is only to be performed by trained specialists. Use spare parts and consumables supplied by CAMAG only. The warranty is voided if parts from other sources are used. Check the service manual before you start service to reduce product-specific risks •...
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Page 5: Parts Supplied
1.3 Further accessories Edwards vacuum pump Optionally a kit containing an Edwards oil sealed rotary vane vacuum pump RV3 (2-phase 220V, operating voltage 100-120V or 220-240V selectable, 50/60 Hz) CAMAG order #022.8880 can be supplied including following parts: Part no Description 343.2100... -
Page 6: Vacuum Connection Kit
Vacuum tube, 2m red Ø 10/20 mm 664.2101 Centring ring DN10KF 672.3101 Clamp ring DN10/16KF Additional metal vacuum hose Additional metal vacuum hose can be supplied under the CAMAG order #140.8880 and contains: Part no Description 672.3102 Flexible vacuum hose DN10KF, 1 m, metal 664.2101... - Page 7 • A vacuum pump with sufficient capacity. If the recommended Edwards oil sealed rotary vane vacuum pump RV3 has been supplied by CAMAG, all necessary connections are provided. If the AMD system is to be operated with a different type vacuum pump, note that the minimum average pumping capacity of 3 m3/h according to PNEUROP and a final pressure of <10mbar are required.
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Page 8: Getting Started
Getting started 3 Getting started 3.1 The AMD 2 The AMD 2 instrument The actual developing chamber is situated in the front part of the housing. Five solvent bottles (1-5) are arranged in front. A conditioning bottle (A) and a solvent waste bottle (B) are integrated in the hood. - Page 9 In the chamber the solvent trough may remain filled with solvent. To remove the solvent and dry the chamber, press the END key. Display Line1 Line 2 Description <RUN> STARTS AMD 2 PROGRAM System ready message. WAITING FOR SYSTEM PRESSURE CLOSE System is waiting for N2-pressure or closing of the lid. PLATE CHECK...
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Page 10: Filling The Bottles
3.3 Initial rinsing The AMD 2 is a powerful tool for determining small amounts of impurities. Handle the instrument, solvents and plates carefully in order to avoid trouble with false peaks (blanks). -
Page 11: Insert A Plate
Getting started • Open visionCATS and double click on the AMD2 icon • Go to the subtab “Manual control” • Set “number of rinsing press” to 3 • Click on “Start” • Activate the tick box “Empty solvent tubes only” •... -
Page 12: Application Hints
Application hints HPTLC plate positioning device with HPTLC plate and plate holder • Now the plate holder containing the correctly positioned HPTLC-plate can be inserted into the separation chamber of the AMD2 device Make sure the layer/sorbent side of the HPTLC plate faces towards the backside of the instrument. - Page 13 Application hints Example of an universal gradient from methanol to n-hexane based on dichloromethane Unlike a gradient in column liquid chromatography, the AMD gradient starts with the strongest, in this case the most polar solvent and is varied toward decreasing elution strength.
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Page 14: Gradient Optimization
Application hints the lowest elution strength „decreasing solvent“ (decreasing the elution strength). The „base solvent“ governs the selectivity of a universal gradient. Examples for a universal gradient are listed below: Increasing solvent Base solvent Decreasing solvent methanol dichloromethane n-heptane methanol t-butyl methyl ether n-heptane acetonitrile... - Page 15 Application hints • A reduction of AMD running times by up to 50 % can be reached by using "extra thin" (100 μm) layers which require only 2 mm migration increments and shorter drying times. Use of Licrosphere plates reduces developing times as well. •...
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Page 16: Maintenance And Service
Additionally to a slight polarity gradient a pH gradient can be employed in the AMD 2 system. This means adding e.g. a small volume of a base to the first bottle content and a small volume of an acid to the third bottle content. The content of bottle 2 is kept neutral. -
Page 17: User Maintenance
5.3 User maintenance User maintenance procedure which should be performed once a week (depending on the usage): AMD 2 • Check proper horizontal levelling of the AMD2 device by means of the spirit level supplied. Placed it on top of the closed chamber lid and level the instrument by rotating the 4 adjustable feet at the bottom of the instrument •... -
Page 18: Service Maintenance
The instrument may only be serviced by authorized technicians who have been properly trained. In addition to the aforementioned user maintenance, CAMAG strongly recommend that maintenance be performed at least once a year by CAMAG authorised service personnel. Regular service and maintenance will ensure that the instrument performs according to CAMAG specifications. -
Page 19: Troubleshooting
Maintenance and Service Maintenance interval of vacuum pump in use Maintenance Refer to the respective manual / suppliers recommendation IQ/OQ CAMAG recommends no IQ/OQ as the pump is tested with the AMD2 IQ/OQ 5.6 Troubleshooting 1. Migration distances are not reproducible: •... -
Page 20: Technical Data
49 30 516 55 50 · Fax 49 30 795 70 73 · E-Mail: infoberlin@camag.com CAMAG Scientific Inc. (USA) · 515 Cornelius Harnett Drive · Wilmington, NC 28401 Telephone 800 334 3909 · Fax 910 343 1834 · E-Mail: tlc@camag.com www.camag.com...
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