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CAMAG DBS-MS 500 Instruction Manual

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INSTRUCTION MANUAL
DBS-MS 500

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Summary of Contents for CAMAG DBS-MS 500

  • Page 1 INSTRUCTION MANUAL DBS-MS 500...

  • Page 2: Table Of Contents

    Start the method ..........................27 Setting up a stack extraction method ..................28 Additional screens ........................29 Setting up an extraction with software (Chronos for CAMAG) support ........29 Maintenance and Service ......................30 Cleaning ..........................30 Decontamination ........................30 Declaration of Decontamination ......................
  • Page 3 Introduction Service Maintenance ....................... 33 Troubleshooting ........................33 Change of X/Y drive battery ......................33 Change of extraction head ......................33 Fittings and capillaries ........................34 Installing high pressure fittings ......................34 Fittings – dead volumes ........................34 Fittings – System leaks ........................35 Exchange of capillaries ........................

  • Page 4: Introduction

    (specific extraction solvent mixture). This extract will then be delivered to the HPLC-MS system for further separation and detection as required. Handling and extraction processes are to be designed for maximum robustness and minimum carryover. 1.2 Basic overview Fig. 1: The DBS-MS 500, Overview of functionalities Instruction Manual, Sep-18...
  • Page 5 The DBS card is only accepted for analysis if all parameters comply. CAMAG has incorporated its knowhow of more than a decade of optical HPTLC image analysis to build this precise and reliable OCR-module.

  • Page 6: Fluidic Principle

    Introduction 1.3 Fluidic principle Fig. 2 shows the fluidic principle diagram with loop valve (V2) in position “Elution”. The Elution pump presses the elution solvent through the ambos and DBS card into the DBS- MS500 loop (connection 3 and 8 of loop valve). Meanwhile the flow of the LC pump is bypassed (through connection 4 and 7).

  • Page 7: Precautions

    Introduction to ambos. The rinsing unit is placed in-between the ambos and the extraction head. The rinsing solvent is cleaning the ambos and extraction head. Fig. 3 Fluidic principle diagram in position “Inline” 1.4 Precautions • Please read this operating manual before starting the installation! This manual contains information and warnings the user has to follow to ensure reliable operation of the instrument •...
  • Page 8 1 and has been designed for indoor use only (IP 20). Further, this device has passed the CAMAG Quality Assurance tests and has been delivered in safe operation condition. For detailed instrument data see chapter technical data •...
  • Page 9 Introduction • Spare fuses must be of the type specified by the instrument manufacturer. It is forbidden to short-circuit or manipulate fuses • Use only the original, with the instrument delivered power cord type • If the instrument is found to be defective, it must be switched off and steps must be taken to ensure that it cannot be switched on by mistake •...

  • Page 10: Parts Supplied

    Introduction 1.5 Parts supplied DBS-MS 500 basic, parts supplied with the instrument Part no Units Reference 050.0705 Chronos Software license 110.5115 HMI (user interface) 115.5139 Waste canister 10L GL45 including tube/filter 115.5110 Rack 125.1035 Cable to HMI 125.5152 Contact closure cable 305.00xx...

  • Page 11: Unpacking/Installation

    Unpacking/Installation 2 Unpacking/Installation 2.1 Unpacking Check with reference to the parts supplied section whether any parts are missing. Lift the instrument carefully out of its packaging. 2.2 Installation environment The place for installation should meet the following requirements: Connections 115 / 230V AC; 50 / 60Hz; min. 130W; A grounded outlet should be within 2 meters of the instrument Contact closure connector HMI connection...

  • Page 12: Removal Of Shipping Protection

    Unpacking/Installation Other requirements: • Do not place the instrument in a location where the temperature undergoes significant changes (e.g. under an air conditioning duct or by a window) • Significant changes in temperature will affect the performance of the unit •...
  • Page 13 Unpacking/Installation 5. Move the plate of the shipping protection to the front and 6. Mont the spring of the gripper arm Instruction Manual, Sep-18...

  • Page 14: Installation Of The Instrument

    5 and 6 • Connect the MS with 10-32 fittings to Valve 2, port 7; according the below figure CAMAG strongly recommends keeping the connection as short as possible (no longer then 2m) to maintain measurement accuracy and quality. Instruction Manual, Sep-18...
  • Page 15 Unpacking/Installation Fig. 5: Fluid Sel F-V1 /Loop F-V2/ CDM F-V3 valvs Port 4: From LC Pump Port 7: To LC/MS system Fig. 6: Loop valve F-V2 connection scheme Instruction Manual, Sep-18...

  • Page 16: Electrics

    Unpacking/Installation Electrics Fig 7. Electric connection panel • Connect the HMI with the instrument (red) • Connect the air or Nitrogen pressure (>5.5 bar up to 8 bar) with a 6mm outer diameter hose (purple). Make sure you keep the length of the hose as short and the diameter as big as possible •...

  • Page 17: Priming

    NO = normally open IN+ = optical input positive (24V) IN- = optical input ground (GND) • Connect the power cord (use only the original delivered CAMAG power cord) • Connect the LAN cable to the computer (for use of CHRONOS software) •...
  • Page 18 Unpacking/Installation Priming of IS circuit • Choose “manual” • Choose “Internal Standard” • Choose the appropriate IS Bottle • Set the no of Prime Cycles to 5 • Set the destination parameter to “to waste” • Activate the priming • Set the destination parameter to “to Needle”...

  • Page 19: Getting Started

    Getting started 3 Getting started 3.1 The HMI (Human-Machine interface) The HMI allows you to control and set all parameters of the DBS-MS500. Not active Active for user Switch on Setup Screens Main Screen Start Start Open Method Manual Service Stack Editor Stack Method...

  • Page 20: Setting Up An Extraction With Hmi Support

    Getting started 3.2 Setting up an extraction with HMI support • Mount the DBS cards (upside down, front side with DBS spots facing towards the back of the instrument) to extract into the racks (in a continuous way) Fig.9: DBS card (front side, upside up) •...

  • Page 21: General Method Settings

    Getting started General method settings • Choose “Method Editor” Fig. 11: Method editor screen • Enter a description, Editor Name and Company if applicable Sample settings • Choose “Sample List” Fig.12: Sample List screen Instruction Manual, Sep-18...
  • Page 22 Getting started Settings: Spot to extract (touch the spot to extract, active ones are black) Start: (Rack 1 - 5; Card 1 - 100) End: (Rack 1 - 5; Card 1 - 100) Card positions: Every 10th position is marked with a metallic sleeve Fig.13: card and rack position (seen from front side of the instrument) No of samples per card: Informs about the maximum number of extractions at one card (up to 4 extractions)
  • Page 23 Getting started Fig.14: General screen Settings: LC-MS-Cycletime: Expected shortest cycle time when no information from the measurement system is received to start new cycle Spot Type: Blood Plasma Blank Card Type: Manufacturer cards Custom Card Config Custom Cards: This screen sets the criteria for customized cards. Instruction Manual, Sep-18...
  • Page 24 Getting started Fig.15: Custom Card screen Settings: Spot to Spot: set distance between spots (for standard DBS cards this is 13.7mm) Offset X: Offset value for left-right direction Offset Y: Offset value for top-bottom direction With dashed ring: Set it active when card has dashed rings Dashed ring diameter: Set to the actual diameter of the dashed ring With Checkboxes: Activate if checkboxes are used •...

  • Page 25: Image Processing Settings

    Getting started Image processing settings • Activate “Image Processing” if applicable • Choose “properties” next to “Image Processing” Fig.16: Image processing screen Settings: Barcode: Choose the applicable barcode Code 128 ITF 14 No barcode Check limits, but extract anyway: If active, the instrument extracts the spot although the spot is not within the below specified specification limits Checkbox Test: Checkboxes are taken into account if active Extraction Test: Extraction is checked if active...

  • Page 26: Internal Standard Settings

    Getting started Internal Standard settings • Activate “Internal Standard” if applicable • Choose “properties” next to “Internal Standard” Fig.17: Internal Standard screen Settings: IS Bottle No: Choose Is bottle for IS application IS-Dry Time: Time between spraying IS and extraction Cleaning Time: Time after each IS application to wash the needle and spray nozzle channel Application Speed and Volume: Choose the appropriate speed and Volume for IS...

  • Page 27: Extraction Settings

    Getting started Extraction settings • Activate “Extraction” if applicable • Choose “properties” next to “Extraction” Fig.18: Extraction screen Settings: Elution Process Flow Rate: Flow rate for the extraction process Volume: Volume to be eluted Bottle No. E: Choose the appropriate bottle for the extraction solvent Rinsing Process •...

  • Page 28: Start The Method

    Getting started Number of Cycles: Choose the appropriate number of rinsing cycles Path through: Inlet: The system is cleaned with the extraction pump through the inlet capillary. (No time can be chosen, one filling volume of syringe per cleaning process, 250µL) Bottle No.

  • Page 29: Setting Up A Stack Extraction Method

    Getting started 3.3 Setting up a stack extraction method Multiple methods can be stacked to perform them in one run. Therefore you have to set up the methods and name them respectively. Fig.20: Method Stack screen • Choose “Stack Editor” •...

  • Page 30: Additional Screens

    Abort: Ends the method 3.5 Setting up an extraction with software (Chronos for CAMAG) support Chronos (by Axel Semrau GmbH& CO KG) for CAMAG is a powerful software for efficiently automating complex sample preparation/acquisition steps with CAMAG DBS samplers. Several data system interfaces allow integration into existing systems. Chronos for CAMAG exchanges data with other systems and controls all of the processes.

  • Page 31: Maintenance And Service

    Maintenance and Service 4 Maintenance and Service 4.1 Cleaning Clean the instrument on a regular base in an appropriate way to assure problem-free functionality over a long period. A good way to perform a cleaning would be to follow the decontamination instruction outlined below.

  • Page 32: Declaration Of Decontamination

    5) Demount the waste bottle B5 and wash it, the bottle has to be shipped back in a non- mounted state. 6) Mount the extraction head back to the plunger and close all covers of the DBS-MS 500. I hereby confirm that I performed a decontamination of the DBS-MS 500 according to the above protocol.

  • Page 33: User Maintenance

    Maintenance and Service 4.3 User maintenance Regular check/maintenance (at least all 3 month) by the user is strongly recommended. User maintenance procedure: • Check the catch basin at the back of the instrument (underneath the fluidic). It has to be completely dry; no signs of liquid should be present. If there are signs of liquid stop operation, check the fluidic connections for leakage and replace the defective parts.

  • Page 34: Service Maintenance

    4.4 Service Maintenance A regular maintenance is mandatory to keep the instrument in perfect working condition. CAMAG recommends executing regular maintenance by a qualified service person, for in detailed information about service and replacement cycles, check the Maintenance Data Sheet (attachment A). If the system works in a high throughput environment, shorter...

  • Page 35: Fittings And Capillaries

    Remove the fitting to confirm the ferrule is swaged onto the tube. • Because the ferrule is permanently attached to the tubing CAMAG highly recommends that the fitting only be used in the receiving port into which it was initially swaged. Failure to this may result in dead volume and/or leaks.

  • Page 36: Fittings - System Leaks

    Maintenance and Service Mixing chamber The tube The tube is too short Fig.23: Potential dead volumes in capillary-based applications Fittings – System leaks Before you even see your first drip from a fitting, your system can tell you that the problem exists.
  • Page 37 Maintenance and Service Make sure you are using a nut and ferrule that are compatible with each other and with the components of your system Check the condition of the nut and ferrule After repeated use, nuts (and especially ferrules) will gradually become deformed to the point of being incapable of creating the seal they were designed to make.

  • Page 38: Exchange Of Capillaries

    Technical data Exchange of capillaries If capillaries or tubing have to be exchanged, only CAMAG original spare parts have to be used as replacement. Color table of capillary color Inner diameter Outer diameter Order no yellow 0.18 mm 1/16” 696.0003 blue 0.25 mm...

  • Page 39: Appendix A: Maintenance Data Sheet

    Appendix A: Maintenance Data Sheet 6 Appendix A: Maintenance Data Sheet The maintenance data sheet informs about maintenance interval of the instrument. In addition, it identifies consumable parts with the respective replacement cycle. Maintenance interval User Maintenance 3 Month for average throughput Service Maintenance 6 Month for average throughput Consumable parts...
  • Page 41 Notes: __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________...

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