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Summary of Contents for CAMAG DBS-MS 500
- Page 1 INSTALLATION GUIDE DBS-MS 500...
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Page 2: Table Of Contents
Electrics ............................8 Software ............................8 Priming ............................. 9 Hardware Connections ....................... 10 CAMAG DBS-MS500 contact closure ................... 10 Connectors on measurement systems .................. 11 Agilent APG remote interface ......................11 AB Sciex AUX/ I/O interface ......................12 Shimadzu event interface ......................12 Waters Xevo TQD with AQUITY UPLC system ................ -
Page 3: Introduction
Introduction 1 Introduction The main function of the DBS-MS 500 is the fully automated solvent extraction of dried blood spot samples for detection by a mass spectrometer. The samples are delivered on cards, with up to four spots per card. To ensure reliable automated sample extraction and handling, these cards are stored in removable racks which hold them in exactly defined positions inside the instrument. -
Page 4: Parts Supplied
Check with reference to the parts supplied section in the instruction manual for complete shipment of instrument and accessories. 2 Unpacking/Installation • Check the installation environment according the Environment Specification (CAMAG SOP 402) • Lift the instrument with the necessary care (4 persons) out of its packaging. - Page 5 Unpacking/Installation 5. Move the plate of the shipping protection to the front and 6. Mont the spring of the gripper arm Installation guide, Nov-18...
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Page 6: Installation Of The Instrument
• Connect the MS with 10-32 fittings to Valve 2, port 7; according the below shown figures CAMAG strongly recommends keeping the connection as short as possible (no longer then 2m) to maintain measurement accuracy and quality. Installation guide, Nov-18... - Page 7 Unpacking/Installation Fig. 2: Valves:Fluid Selection F-V1 /Loop F-V2/ CDM (Controlled Delivery Module) F- Port 4: From LC Pump Port 7: To LC/MS system Fig. 3: Loop valve F-V2 connection scheme Installation guide, Nov-18...
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Page 8: Electrics
Connect the LAN cable to the computer (for use of CHRONOS software) • Switch on the unit Software Check the user manual of Chronos (by Axel Semrau GmbH& CO KG) for CAMAG for computer requirements and installation of the software. Installation guide, Nov-18... -
Page 9: Priming
Unpacking/Installation Priming Prior to first use or after a longer period of non-using the instrument, the fluidic has to be filled with the respective solvents (Priming). Priming of extraction circuit • Choose “Manual” • Choose “Extractor” • For the extraction pump (Prime EX): If the system was completely empty, priming can take up to 50 cycles •... -
Page 10: Hardware Connections
Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements. CAMAG DBS-MS500 is simply to include into an LC/MS System similar to an Autosampler and uses a contact closure connector to communicate with other parts of the system. -
Page 11: Connectors On Measurement Systems
Hardware Connections 3.2 Connectors on measurement systems Agilent APG remote interface Agilent uses the APG Remote Interface; see following drawings and definitions: Installation guide, Nov-18... -
Page 12: Ab Sciex Aux/ I/O Interface
Hardware Connections AB Sciex AUX/ I/O interface AB Sciex uses the AUX I/O Interface (8); see following drawings and definitions: Shimadzu event interface You have to connect a cable between the MAN. INJ. terminal and Dbs500 out signal “Loop is Inline”; see following pictures: Back side of Shimadzu Controller CBM-20A/20Alite: Installation guide, Nov-18... -
Page 13: Waters Xevo Tqd With Aquity Uplc System
Hardware Connections Waters Xevo TQD with AQUITY UPLC system For remote control you have to use Connectors I/II of respectively used device (Solvent Manager or Xevo TQD Mass spectrometer), see following pictures: Installation guide, Nov-18... -
Page 14: Thermo System Under Xcalibur
Hardware Connections Output signal “Stop Flow” could be used as an “Error” signal to stop DBS-MS 500. Switch 2-4 are software configurable signals and could be used to send a “Start Next Sample” signal to the DBS-MS 500. Connect in minimum the DBS-MS 500 signal “Loop is Inline”... - Page 15 Hardware Connections Thermo connector to the LC pump Remote control from LC (output 2) to MS (start in) Installation guide, Nov-18...
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Page 16: Connection Examples
Near the main power socket the 12 Pin Contact/Closure Interface Connector of CAMAG DBS-MS500 can be found. For a minimum wiring you have to connect pin 2/3 of CAMAG connector to pin 1/3 of APG Remote Interface or to pin 21/22 (bridge between pin 9/10) of Sciex AUX I/O connector. - Page 17 Hardware Connections Installation guide, Nov-18...
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Page 18: Lc/Ms System Of Waters And Sciex
Near the main power socket, the 12 Pin Contact/Closure Interface Connector of CAMAG DbsMs500 can be found. For a minimum wiring you only have to connect wires on Pin 2/3. Do not connect Waters Interface and Sciex AUX I/O in parallel. -
Page 19: Thermo System Under Xcalibur
Hardware Connections Thermo system under Xcalibur Note that DBS-MS 500 port 3, 6 and 12 are connected internally within the plug. 3.4 Coupling a PAL fraction collector Installation of a PAL fraction collector on the DBS-MS500 • Unpacking and installing the hardware •... - Page 20 Hardware Connections o Fluidic capillaries (setup can be changed by adapting the PAL method in the script editor, not recommended): • Insert a 100 µl syringe into the dilutor and prime it • Connect the interface cable (remote cable) with the DBS-MS500 Pin PAL Description Wire color...
- Page 21 Hardware Connections • Open the PAL executor (program on “PAL data for installation and analysis (copy to stick)”) o Enter the IP address • Load “PAL_method.xml” and define all parameters; • Create or load a sample list and convert it to a job list •...
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Page 22: Software Coupling
Connecting the DBS to a computer which is not on a network o Disable wireless o Enter an IP address in the range of the DBS-MS 500 IP address at the local area connection properties of TCP/IPv4. o Enable and disable the LAN connection •... -
Page 23: Masslynx Coupling
Software coupling o Enter a IP address in the range of the IP address of the DBS-MS 500 at the local area connection properties of TCP/IPv4 (shown under “Connecting the DBS to a computer which is not an a network”). -
Page 24: Analyst Coupling
There are additional steps for the installation of Chronos in combination with Sciex devices. • Hardware configuration in Analyst Define new profile for the DBS-MS 500 with the according hardware setup 2. Run triggered by 1. Select LC controller extrernal device •... - Page 25 Software coupling Define the LC- synchronization in the LCMS method Uncheck auto-equilibration • Starting a run 1. Start a run here, job 2. System is waiting for list needs to be empty external trigger signal 2.1 LCMS is ready • The Chronos data file names should have the ending .wiff •...
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Page 26: Xcalibur Coupling
Software coupling • Check Chronos if the acquisition service was integrated (settings/ instrumental) 4.4 Xcalibur coupling Go to instrument configuration Configure the LC Installation guide, Nov-18... - Page 27 Software coupling Add the pump: Installation guide, Nov-18...
- Page 28 Software coupling Within the pump setup, select input 1 and Relay 2 Add the remote inject task Installation guide, Nov-18...
- Page 29 Software coupling With inject port 1 With inject port 1 Installation guide, Nov-18...
- Page 30 Software coupling Exit the setup and select controlled by external autosampler Installation guide, Nov-18...
- Page 31 Software coupling Open Xcalibur and program a LC method with inject and a relay tasks: • Put the LCMS in a “ready” state: 4) Simulate start signal • (DBS service tool) Go to Service-> I/O signals-> fluidic-> give signals by activating “Lp Inline”...
- Page 32 Chronos standard method CAMAG (Switzerland) · Sonnenmattstrasse 11 · CH-4132 Muttenz 1 Telephone +41 61 467 34 34 · Fax +41 61 461 07 02 · E-Mail: info@camag.com CAMAG (Germany) · Bismarckstraße 27-29 · D-12169 Berlin Telephone 49 30 516 55 50 ·...
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