Related Manuals for logos biosystems LUNA II
Summary of Contents for logos biosystems LUNA II
- Page 1 Catalog No.: L40001 / L40002 Document No.: L44001 Revision Date: December 02, 2014 SW Version: 1.4.4...
- Page 2 IMPORTANT The LUNA-II™ Automated Cell Counter is a Laboratory Electrical Instrument for Scientific Research Use Only, BUT NOT A MEDICAL or THERAPEUTIC or IN VITRO DIAGNOTICS DEVICE.
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Page 3: Table Of Contents
Table of Contents Safety Information ---------------------------------------------------------------------------------------- General Guidelines -------------------------------------------------------------------------------------- Environment Conditions -------------------------------------------------------------------------------- Chapter 1 – Introduction ---------------------------------------------------------------------- 1.1 Product Overview -------------------------------------------------------------------------------- 1.2 Key Features -------------------------------------------------------------------------------------- 1.3 Product Contents --------------------------------------------------------------------------------- 1.4 Product Specifications -------------------------------------------------------------------------- 1.5 Product Description ------------------------------------------------------------------------------ Chapter 2 – Setting up -------------------------------------------------------------------------- 2.1 Installation ------------------------------------------------------------------------------------------ 2.2 Start-Up Screen -----------------------------------------------------------------------------------... - Page 4 4.8 After Counting: Calculation for Subsequent Experiments ------------------------------ Chapter 5 – Focusing Option --------------------------------------------------------------- 5.1 Autofocusing --------------------------------------------------------------------------------------- 5.2 Manual Focusing --------------------------------------------------------------------------------- Chapter 6 – Review the Previous Results ------------------------------------------ Chapter 7 – Maintenance and Troubleshooting --------------------------------- 7.1 Turn On/Off LUNA-II™ -------------------------------------------------------------------------- 7.2 Cleaning -------------------------------------------------------------------------------------------- 7.3 Calibrating the Touchscreen ------------------------------------------------------------------- 7.4 Calibrating LUNA-II™...
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Page 5: Safety Information
Safety Information For best results with the LUNA-II™ Automated Cell Counter, follow the instructions below in addition to the general precautions for using electrical instruments. 1. Be careful to avoid electric shock while operating the instrument. Do not touch it and other components with wet hands. - Page 6 <Symbols used in this User Manual> The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that users of this instrument have the responsibility of returning and disposing of WEEE in an ecologically friendly manner. Follow waste ordinances of your region for proper disposal provisions.
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Page 7: General Guidelines
General Guidelines for Using the LUNA-II™ Automated Cell Counter In order to achieve the best results with the LUNA-II™ Automated Cell Counter, follow the instructions below carefully. The instrument must be operated in compliance with the operating environment described on page 9. -
Page 8: Environment Conditions
Environment Conditions 100 ‒ 240 VAC, 1.2 A Operating Power Frequency 50/60 Hz Electrical Input 12 VDC, 3.3 A Installation Site Indoor use only 10 ‒ 35℃ Operating Temperature 20 ‒ 80% Maximum Relative Humidity ≤2,000 m Altitude Pollution Degree... -
Page 9: Chapter 1 - Introduction
Due to several innovations introduced by Logos Biosystems, LUNA-II™ provides a state of the art cell counting device and eliminates the tedium and subjectivity of manual cell counting. -
Page 10: Key Features
1.2 Key Features Key features Description Small footprint Compact size with light weight saves space and is suitable for either laboratory table or biosafety cabinet. Accuracy & precision With sophisticated optical components and counting algorithm, LUNA-II™ provides optimized and reproducible results every time. -
Page 11: Product Contents
1.3 Product Contents The product package of the LUNA-II™ Automated Cell Counter contains the following components. Component Quantity LUNA-II™ Automated Cell Counter (with or without printer) Power cord (including an adapter) Luna™ Cell Counting Slides 1 box (50 slides for 100 counts) Trypan Blue Stain (0.4 %) 2 x 1 ml Luna™... -
Page 12: Product Specifications
1.4 Product Specifications 1.4.1. LUNA-II™ Automated Cell Counter specifications Instrument Type Benchtop cell counter Dimensions (WxDxH) 28 cm (6.3 11.0 inch) Weight 1.6 kg (3.5 lb) without the external power adaptor ‒ 1 x 10 Cell Concentration Range 5 x 10 cells/ml 3 ‒... -
Page 13: Product Description
1.5 Product Description 1.5.1 Front view of the LUNA-II™ Automated Cell Counter The front view of the LUNA-II™ Automated Cell Counter shows various parts as shown below. Wide touchscreen located in the upper front of the instruments contains buttons for all functions needed to operate the instrument and displays data acquired. - Page 14 1.5.3 Right side view of the LUNA-II™ Automated Cell Counter The right side view of the LUNA-II™ Automated Cell Counter shows a built-in printer (L40001 model only) which allows rapid printing the cell counting results. 1.5.5 Luna™ Cell Counting Slide The Luna™...
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Page 15: Chapter 2 - Setting Up
Chapter 2 – Setting up 2.1 Installation Upon receiving the product package, unpack it carefully and ensure that every component is included and no damage has been occurred. Place the LUNA-II™ Automated Cell Counter on a flat and stable surface. Insert one end of the power cord into the instrument and plug the other end of the power cord into an electrical outlet after checking the outlet configuration in your local area. - Page 16 LOADING sign of the LUNA-II™ Automated Cell Counter. The Start-Up screen will be displayed on the touchscreen as shown below in 2.2. See Section 7.1 for Turn on and Turn off the instrument.
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Page 17: Start-Up Screen
2.2 Start-Up Screen The Start-Up screen will show 5 compartments; Power icon, count, review, protocol, and settings. The Power icon in the Start-Up screen can be used to turn off the instrument. See Section 7.1. for further information to turn on/off the instrument. -
Page 18: Settings
2.3 Settings Generally, there is no need to change the settings of the instrument since they are preset at the time of manufacture. If users need to reset the date, time, or other options, users can adjust or change the options/parameters in the “Settings”... - Page 19 The Settings menu allows you to perform the followings: Change options for cell counting by pressing [Counting Options]. Set the date and/or time by pressing [Date / Time]. Re-calibrate the instrument by pressing [Calibrate]. Update the software by pressing [Update Software]. ...
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Page 20: Calibrating The Touchscreen (Calibrate Touchscreen)
2.4 Calibrating the Touchscreen (Calibrate Touchscreen) This function can be used when the response of touchscreen is abnormal. On the Settings screen, press [Calibrate Touchscreen] as below. Now, users can see the following screen. If users want to calibrate the touchscreen, press [OK]. If not, press [Cancel]. - Page 21 Now, small cross mark will be appeared in the upper left corner in the grey screen as below. Press the cross mark, then the cross mark will move to the upper right corner as below. Press the cross mark again. The mark will move to lower right corner of screen. Press it again. The cross mark will further move to lower left corner and center of screen after each touch.
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Page 22: Changing Options For Cell Counting (Counting Options)
2.5 Changing Options for Cell Counting (Counting Options) The LUNA-II™ Automated Cell Counter provides two options for cell counting: “With Trypan Blue” or “Without Trypan Blue”. This function provides inter-change of these options. Option Description With Trypan Blue* This option can be used for regular bright field counting, when cell samples are mixed with 0.4% trypan blue stain in a 1:1 ratio. - Page 23 In the Counting Options screen, current option will be marked as blue √ mark as below. The counting option can be easily changed by pressing un-selected option. If [Without Trypan Blue] is chosen, the following screen will be displayed.
- Page 24 If [With Trypan Blue] is chosen, the following sign will be displayed. The Counting Option is simply changed by pressing [OK]. Otherwise press [Cancel] to return the previous option. Press [<] in the upper left corner to move to the Settings screen.
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Page 25: Setting The Date And Time
2.6 Setting the Date and Time The LUNA-II™ Automated Cell Counter provides current date and time for record keeping. Users may want to change the date and time, since factory setting is adjusted to the local time of Korean. Once the date and time are set, no additional setting is required for routine laboratory use. On the Settings screen, press [Date / Time] as below. -
Page 26: Calibrating Luna-Ii™ (Calibrate)
2.7 Calibrating LUNA-II™ (Calibrate) Background calibration is a prerequisite for successful detection of cells. The LUNA-II™ Automated Cell Counter provides easy-to-use automatic self-calibration of background. On the Settings screen, press [Calibrate] as below. The following screen will be displayed. For the Calibration Step 1, the counting slide port should be emptied. As directed on the screen, remove the counting slide from the counting slide port of the instrument. - Page 27 Following screen will be displayed with increasing red bar over the time. The Calibration Step 1 will take a few seconds. Do not turn off the instrument. During the process, prepare diluted trypan blue solution by mixing 0.4% trypan blue stain with equal volume of either distilled water, phosphate buffered saline (PBS), or plain medium.
- Page 28 Press [Start] to initiate the Calibration Step 2. Following screen will be displayed with increasing red bar over the time. Do not turn off the instrument. After completing calibration, the instrument will display following sign on the screen. Press [Exit] to move to the Settings screen.
- Page 29 Now, the date of last calibration is changed (Compare to the Settings screen in page 26).
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Page 30: Updating The Software (Update Software)
The current version of software can be identified either in the Initializing screen (see page 15) or the Settings screen as above. The up-to-date software, consisting of single file, can be downloaded from Logos Biosystems Website (http://www.logosbio.com). This software should be saved into the root directory of the USB drive. -
Page 31: Chapter 3 - Protocol Setting
Chapter 3 – Protocol Setting The LUNA-II™ Automated Cell Counter provides a basic (DEFAULT) protocol which can be used for most common cell lines. In addition, LUNA-II™ also provides tools to setup individual protocols. All the parameters in the protocol can be modified by users and saved as alternative protocol. Users can save up to 300 protocols. - Page 32 Min. & Max. Cell Size: Average size of cells also may vary from cell type to cell type. With this parameter, users can optimize the instrument to efficiently detect their own cells. The value can be adjusted with 1 µm of increase per step. Declustering Level: Cultured mammalian cells may form clumps during culture or handling.
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Page 33: Setting The Protocols
3.2 Setting the Protocols In the Start-Up screen, press [protocol] as below. In the Protocol screen, users can identify all the parameters as below. The selected protocol is marked with white letters and blue back ground in the left panel of screen. The value of each parameter is displayed in the right panel of screen. - Page 34 Note: The factory setting DEFAULT protocol cannot be modified. Press other protocol or [New Protocol] and press [Load] as below. Now LUNA-II™ display following sign on the screen.
- Page 35 Press [OK]. To delete the selected protocol, press [Delete]. To edit the selected protocol, press [Edit]. After pressing [Edit], the arrows (upward and downward) will be activated and marked as solid arrows as below. Press the arrows to edit the values. Then press [Save as].
- Page 36 Now users can see the Save as screen as below. Using keyboard in the screen, put the desired name and press [Save] in the lower right corner. Now, new protocol named James is displayed in the Protocol panel of Protocol screen.
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Page 37: Selection Of Protocol
3.3 Selection of Protocol To change protocol to be used, press the protocol button in the Set-Up screen. Select desired protocol by pressing the protocol name and press [Load] to apply selected protocol to use. - Page 38 LUNA-II™ will display following sign on the screen. Now the instrument is ready to count cells with selected protocol. IMPORTANT Selecting the protocol name does not mean changing protocol to be used. To use selected protocol, users should press [Load].
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Page 39: Chapter 4 - Counting Cells
Chapter 4 – Counting Cells 4.1 Preparing the Instrument Turn on or activate the LUNA-II™ Automated Cell Counter (See Section 7.1). Press [count] on the Start-Up screen. LUNA-II™ will display the Count screen. In the Count screen, users can identify the Staining option and selected protocol in the upper panel of screen. -
Page 40: Sample Preparation
4.2 Sample Preparation 4.2.1 Materials required Cell suspension Luna™ Cell Counting Slides Trypan Blue Stain 0.4 % Luna™ USB drive or equivalent 4.2.2 Prepare cell suspension according to standard cell biology procedure. Please avoid clumping of cells for accurate counting. 4.2.3 Mix 10 l of the cell suspension with 10 l of Trypan Blue Stain in an appropriate tube by gentle pipetting up and down. -
Page 41: Counting Cells
4.4 Counting Cells 4.4.1 Insert the sample-loaded slide into the counting slide port of LUNA-II™, ensuring that the loaded chamber is inserted into the instrument. LUNA-II™ analyzes only the inserted chamber upon counting. Note: After inserting the slide, LUNA-II™ only reads the inserted chamber. Note: Make sure that the counting slide is not inserted upside-down. - Page 42 The LUNA-II™ Automated Cell Counter provides 1X, 2X, and 4X images as follows. The field of cell image can be navigated by pressing the screen and moving the finger or stylus pen on the image. Two small red boxes in the upper left corner of cell image represent the entire field and field of view, respectively.
- Page 43 4.4.2 Press [Count] to start counting. Now all of the buttons will be inactivated and the bar, which is indicating the progress of cell counting, will be appeared on the screen as below. In general, the counting takes 15 to 22 seconds. LUNA-II™ will display the Cell Counting Results screen as below,...
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Page 44: After Counting: Image View
4.5 After Counting: Image View To view the captured image of cells, press [Image] as below. The Image screen will be appeared as below. Similar to the Count screen, the magnification of the captured image can be changed by pressing the magnifier button on the right of the image. - Page 45 [Tag] in the upper right corner of the image will activate the Tag function. The live cells will be marked by green circles and dead cells will be marked by red circles as below. Note: This “Tag” function is one of the distinct tools of the LUNA-II™ Automated Cell Counter. This function allows the user to review the data immediately to determine the accuracy of the counting without additional manipulation or devices.
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Page 46: After Counting: Histogram & Gating
4.6 After Counting: Histogram & Gating LUNA-II™ provides graphical analyses of the cell counting results. By pressing Histogram & Gating, you can view the Histogram & Gating screen as below. On this screen, users can review the distribution of cells according to their sizes. Green bars represent the live cells and red bars represent dead cells. - Page 47 When pressing [Live/on], the icon will be changes as [Dead/on] and the histogram will display the distribution of dead cells without live cells as below. In addition, when pressing [Cell number] on the left side of histogram, the LUNA-II™ Automated Cell Counter also display the cell concentration.
- Page 48 In addition, the LUNA-II™ Automated Cell Counter provides gating function. Gating function consists of four components: the lower and upper limit and two arrow head in the lower part of screen as below. The lower and upper limit can be activated by pressing as above and adjusted by pressing the icons with arrow head.
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Page 49: After Counting: Saving And Printing The Results
4.7 After Counting: Saving and Printing the Results The LUNA-II™ Automated Cell Counter provides multiple record options: saving the results and/or printing the results. To save or print the results, press [Save/Print] in the Cell Counting Results screen as below. The Save/Print screen will be seen as below. - Page 50 Now, users can save the selected data by pressing [Save] in the lower right corner of screen. IMPORTANT Remember that the “Raw Image” should be turned on to save the “Raw Image” which should be provided to your distributor or Logos Biosystems to get the best technical support.
- Page 51 The LUNA-II™ Automated Cell Counter provides additional option to report the results. Users easily print the Cell Counting Results by simply pressing [Print] in the upper right corner of screen. The printed report will be displayed as below. Cell Count Report File name: HL-60-05092014-1 3350605 Date: 05 Sep., 2014 13:35...
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Page 52: After Counting: Calculation For Subsequent Experiments
4.8 After Counting: Calculation for Subsequent Experiments The LUNA-II™ Automated Cell Counter provides a built-in dilution calculator. Users easily calculate the amount of cell suspension to dilute for subsequent experiments. Press [Dilution] to activate the Dilution Calculator as below. The Dilution Calculator initially shows the measured concentration of total cells from cell counting. Users can select the Current Concentration of Total, Live, or Dead cells by pressing the Black Sign under the value as below. -
Page 53: Chapter 5 - Focusing Option
Chapter 5 – Focusing Option The LUNA-II™ Automated Cell Counter has been integrated with a novel focusing mechanism based on the liquid lens technology. Unlike traditional autofocusing based on mechanical moving of lens in vertical axis, the liquid lens technology enables autofocusing without mechanical moving. With this advance, the LUNA-II™... -
Page 54: Manual Focusing
After pressing [Count], users can obtain autofocused image as below. 5.2 Manual Focusing Although the LUNA-II™ Automated Cell Counter provides autofocusing function, users may want to focus the image manually with various purposes. The LUNA-II™ Automated Cell Counter also provides manual focusing function. Users can adjust the focus manually by simply clicking the arrow heads (up or down) as below with either activating or deactivating autofocus function. -
Page 55: Chapter 6 - Review The Previous Results
Chapter 6 – Review the Previous Results The LUNA-II™ Automated Cell Counter provides stand-alone review function for previous results. Users can easily review the previous results without additional devices. To review the previous results, the USB drive that contains the previous results should be connected to the USB port of the LUNA-II™... - Page 56 In the Review Files, users can select File name to review the results and image. After selecting the file name, the previous cell counting results will be displayed on the right side of screen as below. The captured image with tags can be also reviewed by pressing the small image under the Results section as above.
- Page 57 In the Previous Counts function, users can overview the list of previous counts with summarized results as below. This list can be exported into USB drive as a CSV format. The LUNA-II™ Automated Cell Counter can store up to 1,000 previous counts.
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Page 58: Chapter 7 - Maintenance And Troubleshooting
Chapter 7 – Maintenance and Troubleshooting 7.1. Turn On/Off LUNA-II™ To turn on LUNA-II™, press the POWER button in front of the instrument. The Power icon in the Start-Up screen can be used to turn off the instrument (see page 17). Alternatively, the instrument is turned off by pressing the POWER button in front of the instrument for 5 seconds. -
Page 59: Calibrating The Touchscreen
7.2.3 Decontaminating with alcohol When the instrument needs to be decontaminated, use a soft cloth lightly moistened with 70% alcohol to wipe the outer surface. Never pour or spray alcohol or any other solution directly onto the instrument; this may cause severe damage to the instrument or give an electric shock to users. Note: do not use an abrasive solution or a bleach solution that can cause scratches on the outer surface or the touchscreen. -
Page 60: Troubleshooting
7.6 Troubleshooting Problem Possible Cause Solution Make sure that cells are not clumped. The more Clumped cells single cells, the better counting results. For best results, the concentration of cells should ‒ 1 x 10 Concentration of cells be 5 x 10 cells/ml. - Page 61 Use the USB drive supplied with the instrument. Or, make sure that your USB drive is compatible Incompatible USB drive with the instrument. The version of the USB drive must be 2.0. Some types of USB drives are not detected or compatible with the instrument. More than one software Delete software with previous versions from the version...
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Page 62: Chapter 8 - Ordering Information
Chapter 8 - Ordering Information The following products can be ordered from your regional supplier or the website (www.logosbio.com). Cat # Product Size L40001 LUNA-II™ Automated Cell Counter (with printer) each LUNA-II™ Automated Cell Counter (without printer) L40002 each L12001 Luna™... -
Page 63: Chapter 9 - Purchaser Notification
Logos Biosystems, Inc. (“Company”) will not claim any consideration against the purchaser of infringement of patents owned or controlled by the Company which cover the product based on the... -
Page 64: Instrument Warranty
9.2 Instrument Warranty Logos Biosystems, Inc. (“Company”) warrants to the original purchaser (“Purchaser”) that the instrument (“Instrument”), if properly used and installed, will be free from defects in materials and workmanship and will conform to the product specifications for a period of one (1) year (“Warranty Period”) from the date of purchase. - Page 65 The Company shall have no responsibility or liability for any special, incidental, indirect or consequential loss or damage resulting from the use or malfunction of the Instrument. This limited warranty is sole and exclusive. The Company makes no other representations or warranties of any kind, either express or implied, including for merchantability or fitness for a particular purpose with regards to this Instrument.
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Page 66: Contact Information
For more information or technical support, please call, write, fax, or email. Our regional suppliers are listed on our web page (www.logosbio.com). Logos Biosystems, Inc. Address: Suite 930 Doosan Venturedigm, 126-1 Pyungchon-Dong, Dongan-Gu, Anyang-City, Gyunggi-Do, Republic of Korea (Zip code: 431-755)