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Troubleshooting
Related Manuals for logos biosystems Luna-II yf
Summary of Contents for logos biosystems Luna-II yf
- Page 1 User Manual LBSM-MD-ML-LUY-001 www.logosbio.com VL1712-01...
- Page 2 Do not disassemble the device on any occasion as this will invalidate your warranty. TRADEMARKS The trademarks used in this document are the property of Logos Biosystems, Inc. © 2017 Logos Biosystems, Inc. All rights reserved. The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that users of this instrument have the responsibility of returning and disposing of WEEE in an environmentally friendly manner.
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Page 3: Table Of Contents
1.2 Key Features ------------------------------------------------------------------------------------------------------------------- 1.3 Product Contents ------------------------------------------------------------------------------------------------------------- 1.4 Product Specifications ------------------------------------------------------------------------------------------------------- 1.5 Product Description ---------------------------------------------------------------------------------------------------------- ™ 1.5.1 Front View of the LUNA-II YF ------------------------------------------------------------------------------------ ™ 1.5.2 Rear View of the LUNA-II YF ------------------------------------------------------------------------------------ Chapter 2 – Setting Up -------------------------------------------------------------------------------- 2.1 Installation ----------------------------------------------------------------------------------------------------------------------... -
Page 4: Safety Precautions
Read this manual carefully before you begin to use this instrument to ensure that you know how to operate it safely and correctly. Use the instrument as specified by Logos Biosystems. Keep this manual in an easily accessible location for future reference. -
Page 5: General Guidelines
General Guidelines ™ Follow the instructions below to obtain the best results with the LUNA-II YF Automated Yeast Cell Counter. ™ Hold PhotonSlides by the edges to avoid touching the optical surface. Take care that the optical surfaces of the slide do not become smudged, damaged, or contaminated. -
Page 6: Chapter 1 - Introduction
CSV files and provides the option to generate comprehensive PDF ™ reports with the date, time, protocol used, cell images, and relevant histograms. The LUNA-II YF also provides the option to review previous data that can be exported to USB as a CSV file. -
Page 7: Product Contents
The warranty does not cover damage that may occur during shipping and handling. Any damage claims must be filed with the carrier. Contact your local distributor or Logos Biosystems if anything is missing. 1.4 Product Specifications ™... -
Page 8: Product Description
1.5 Product Description ™ 1.5.1 Front View of the LUNA-II YF ™ The front of the LUNA-II YF has a wide touchscreen, a power button, a slide port to insert slides and a USB port for easy data transfer. Touchscreen... -
Page 9: Chapter 2 - Setting Up
Chapter 2 – Setting up 2.1 Installation ™ Place the LUNA-II YF on a clean and level surface. Connect the power cord to the AC adapter. Connect the power cord to an electrical outlet after checking the outlet configuration in your local area. -
Page 10: Settings
2.3 Settings The instrument is preset at the time of manufacture and may be used immediately. Users may adjust the settings of the instrument as desired. Select SETTINGS from the main menu. The Settings screen displays: a home icon: press this icon to return to the main menu, ... -
Page 11: Settings: Save Options
2.3.1 Settings: Save Options Save Options allows the user to set the type of file(s) saved and the default file name prefix used for subsequent counts. Press Save Options in the Settings screen. There are three saving options. Default Save Description Analyzed Image Tagged image of live and dead cells... -
Page 12: Settings: Dilution Calculator Options
Press < to return to the Settings screen. 2.3.3 Settings: Date/Time ™ The LUNA-II YF uses a 24-hour clock and is preset to Korean Standard Time. Adjust the settings to the local date and time for accurate record keeping. Press Date/Time in the Settings screen. -
Page 13: Settings: Background Calibration
Logos Biosystems continually provides software updates to ensure optimal performance. The existing version of software is displayed in the startup screen and the Settings screen. Download the most recent version from the Logos Biosystems website (www.logosbio.com) into the root directory of a compatible USB drive. -
Page 14: Chapter 3 - Protocol Settings
Chapter 3 – Protocol Settings ™ The LUNA-II YF provides two default protocols that can be used for most common yeast cells. Users may create and save up to 300 unique protocols. 3.1 Protocol Parameters ™ LUNA-II YF protocols have the following parameters:... -
Page 15: Creating And Editing Protocols
3.2 Creating and Editing Protocols Select PROTOCOL from the main menu. The Protocol screen includes a list of saved protocols. The selected protocol is highlighted in blue. The parameters of the selected protocol are displayed in the right panel. The AO-PI and FDA-PI protocols cannot be modified or deleted. To create a new protocol, select one of the default protocols and press Save as. -
Page 16: Protocol Selection
3.3 Protocol Selection When staining cells with Acridine Orange and Propidium Iodide, select the AO-PI protocol. When staining cells with Fluorescein Diacetate and Propidium Iodide, select the FDA-PI protocol. Select the desired protocol in the Protocol screen. Press Load to apply the selected protocol. Now the instrument is ready to count cells with the selected protocol. -
Page 17: Chapter 4 - Counting Yeast Cells
Chapter 4 – Counting Yeast Cells 4.1 Instrument Preparation Select COUNT from the main menu. The set protocol, date, and time appear in the panel at the top of the Count screen. Set the desired protocol prior to counting (see Section 3.3: Protocol Selection). 4.2 Sample Preparation Prepare a yeast suspension according to standard procedures. -
Page 18: Slide Insertion
4.3 Slide Insertion ™ ™ Insert the slide face up and sample-side first into the slide port of LUNA-II YF . The LUNA-II YF can only analyze the inserted chamber. Important! Do not insert the slide facedown. 4.4 Color/Light Adjustment ™... -
Page 19: Focusing
4.5 Focusing ™ The LUNA-II YF has an autofocusing algorithm that works in tandem with a focusing mechanism to rapidly obtain the Z position of the sample by applying a small voltage to a liquid lens. The elimination of mechanical parts in the focusing mechanism removes noise and significantly reduces the need for servicing. -
Page 20: Results
4.7 Results ™ The LUNA-II YF has onboard data analysis software that allows users to analyze data immediately. 4.7.1 Results: Image View *Average size indicates the fluorescent object size. Press Image to view the captured image of the analyzed cell sample. -
Page 21: Results: Histogram And Gating
™ The LUNA-II YF provides a gating function that can be controlled by the gating bar on the bottom of the screen. Select the desired light grey limit icon. The selected icon will become red. -
Page 22: Results: Dilution Calculator
4.7.3 Results: Dilution Calculator Users may use the onboard dilution calculator to compute dilutions for subsequent experiments. Press [Dilution] and the dilution calculator will appear. The dilution calculator starts out with the preset concentration of cells (total, live, or dead) as the current concentration. The current concentration options are Total, Live, Dead, and Custom. -
Page 23: Results: Saving And Printing
4.7.4 Results: Saving and Printing Press [Save/Print] in the Results screen. Select the desired saving options. The files to be saved as default may be set in the Settings (see Section 2.3.1: Settings: Save Options). Using the onscreen keyboard, name the count as desired. A default file name prefix may be set in the Settings (2.3.1 Settings: Save Options). - Page 24 AOPI_07032016_161208.pdf ™ A summary of each count performed is automatically saved to the LUNA-II YF ™ The LUNA-II YF stores up to 1000 counts onboard. ™ Alternatively, press Print (the LUNA Printer is sold separately). The printed report will contain the cell count results and protocol details.
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Page 25: Chapter 5 - Review Previous Results
The review screen has two options: [Review Files] and [Previous Counts]. [Review Files] brings up data from a USB ™ drive and Previous Counts] looks up data stored directly on the LUNA-II YF ™ ™ Insert a USB drive into the LUNA-II YF . -
Page 26: Chapter 6 - Maintenance And Troubleshooting
6.2 Cleaning ™ Turn the LUNA-II YF off and disconnect the power cable before cleaning. Make sure that liquids do not enter any part of the instrument during cleaning. Clean the surfaces of the instrument with a soft cloth dampened with distilled water. Wipe dry immediately. Do not pour or spray liquids directly onto the instrument. -
Page 27: Troubleshooting
Use the supplied or a compatible USB drive. Download the file again Incorrectly saved or into the root directory of the USB drive. Insert the USB and press damaged software [Software Updates] in the Settings screen. If the problem persists, contact your local distributor or Logos Biosystems. -
Page 28: Chapter 7 - Ordering Information
Chapter 7 – Ordering Information Cat # Product Quantity ™ Counter L50001 LUNA-II YF Automated Yeast Cell Counter 1 unit ™ L12005 PhotonSlide , 50 Slides 1 box ™ L12006 PhotonSlide , 500 Slides 10 boxes ™ Slides L12007 PhotonSlide... -
Page 29: Chapter 8 - Purchaser Notification
Logos Biosystems, Inc. (“Company”) will not claim any consideration against the purchaser of infringement of patents owned or controlled by the Company which cover the product based on the manufacture, use or sale of a therapeutic,... - Page 30 Logos Biosystems HEADQUARTERS FL 2 & 3 28 Simindaero 327beon-gil, Dongan-gu Anyang-si, Gyeonggi-do 14055 OUTH OREA Tel: +82 31 478 4185 Fax: +82 31 478 4184 Email: sales@logosbio.com 7700 Little River Turnpike STE 207 Annandale, VA 22003 Tel: +1 703 622 4660...
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