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logos biosystems Luna II User Manual

Second generation automated cell counter
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Catalog #: L10001
Document #: LBSM-MD-ML-LUB-001
Version: VL1712-01

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Summary of Contents for logos biosystems Luna II

  • Page 1 Catalog #: L10001 Document #: LBSM-MD-ML-LUB-001 Version: VL1712-01...

  • Page 2: Table Of Contents

    IMPORTANT LUNA™ Automated Cell Counter is a Laboratory Electrical Instrument for Scientific Research Use Only, BUT NOT A MEDICAL or THERAPEUTIC or IN VITRO DIAGNOTICS DEVICE. Table of Contents Safety Information ----------------------------------------------------------------------------------------------------- General Guidelines --------------------------------------------------------------------------------------------------- Environment Conditions --------------------------------------------------------------------------------------------- Chapter 1 – Introduction ------------------------------------------------------------------------------ 1.1 Product Overview --------------------------------------------------------------------------------------------- 1.2 Product Contents ---------------------------------------------------------------------------------------------...
  • Page 3 Chapter 4 – Reanalyzing Saved Count Results -------------------------------------- 4.1 Importing Previous Counts ---------------------------------------------------------------------------------- 4.2 Recounting ------------------------------------------------------------------------------------------------------ 4.3 “Previous Count” Button ------------------------------------------------------------------------------------- 4.4 Setting up the Protocol for Counting --------------------------------------------------------------------- Chapter 5 – Maintenance and Troubleshooting ------------------------------------- 5.1 Cleaning --------------------------------------------------------------------------------------------------------- 5.2 Changing the Battery ----------------------------------------------------------------------------------------- 5.3 Calibrating the Counter -------------------------------------------------------------------------------------- 5.4 Updating the Firmware --------------------------------------------------------------------------------------- 5.5 Troubleshooting ------------------------------------------------------------------------------------------------...

  • Page 4: Safety Information

    8. Do not disassemble the instrument in any event. If the instrument is malfunctioning or broken, please contact your local distributor or Logos Biosystems. Disassembling the instrument invalidates its warranty. 9. Use only authorized components (adaptor, power cord, and USB drive).
  • Page 5 <Symbols used in this User Manual> The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that users of this instrument have the responsibility of returning and disposing of WEEE in an ecologically friendly manner. Follow waste ordinances of your region for proper disposal provisions.

  • Page 6: General Guidelines

    General Guidelines for Using the LUNA™ Automated Cell Counter In order to achieve the best results with the LUNA™ Automated Cell Counter, users must follow the instructions below carefully. The instrument must be operated in compliance with the operating environment described on page 7.

  • Page 7: Environment Conditions

    Environment Conditions Operating Power 100 - 240 VAC, 1.5A Frequency 50/60 Hz Electrical Input 12 VDC, 3.5A Installation Site Indoor use only Operating Temperature 10 - 35℃ Maximum Relative Humidity 20 - 80% ≤2,000 m Altitude Pollution Degree www.logosbio.com...

  • Page 8: Chapter 1 - Introduction

    Due to several innovations introduced by Logos Biosystems, LUNA™ provides a state of the art cell counting device and eliminates the tedium and subjectivity of manual cell counting.
  • Page 9 Key features of the LUNA™ Automated Cell Counter are as follows: Key features Description Due to its minimal size (22 x 21 x 8.5 cm) & weight (1.2 kg), it Small footprint can be used on a clean bench or stored in a biosafety cabinet for convenience.

  • Page 10: Product Contents

    1.2 Product Contents The LUNA™ Automated Cell Counter in the product package consists of the following components. Component Quantity LUNA™ Automated Cell Counter Power cord (with AC adapter) LUNA™ Cell Counting Slides, 50 Slides (100 Counts) 1 box Trypan Blue Stain, 0.4 % 2 x 1 mL USB Drive, 16 GB When you receive the product package, please immediately check it with the above component list...

  • Page 11: Product Specifications

    1.3 Product Specifications 1.3.1. LUNA™ Automated Cell Counter specifications Instrument Type Benchtop cell counter Dimensions (WxDxH) 22 x 21 x 8.5 cm Weight 1.2 kg – 1 x 10 – 1 x 10 Cell Concentration Range 1 x 10 cells/mL (Optimally 5 x 10 cells/mL) Cell Diameter Range 5 - 60 µm...

  • Page 12: Product Description

    1.4 Product Description 1.4.1 Front view of the LUNA™ Automated Cell Counter The front view of the LUNA™ Automated Cell Counter shows a wide touch screen. This interface contains buttons for all functions and displays results. 1.4.2 Rear view of the LUNA™ Automated Cell Counter The rear view of the LUNA™...
  • Page 13 1.4.3 Right side view of the LUNA™ Automated Cell Counter The right side view of the LUNA™ Automated Cell Counter shows a focus knob and a slide port. Use the focus knob to get optimized cell images by adjusting contrast between live cells with bright centers and dead cells with dark centers.
  • Page 14 1.4.5 LUNA™ Cell Counting Slide The plastic disposable Cell Counting Slide consists of 2 chambers, labeled as A and B, which can be used for the same sample as duplicates or for 2 different samples. The depth of the counting chamber is 100 µm.

  • Page 15: Chapter 2 - Setting Up

    Chapter 2 – Setting up 2.1 Installation 2.1.1 Upon receiving the product package, unpack it carefully and ensure that every component is included and no damage has occurred. 2.1.2 Place the LUNA™ Automated Cell Counter on a flat, stable surface. 2.1.3 Insert one end of the power cord into the instrument and plug the other end of the power cord into an electrical outlet after checking the outlet configuration in your local area.

  • Page 16: Start-Up Screen

    2.2 Start-Up Screen When the cell counter is turned on, the Start-Up screen is displayed. The Start-Up screen will show 7 compartments; menu, display, protocol & date, function, zoom, staining status, and setting. <Start-Up Screen of the LUNA™ Automated Cell Counter> Note: While operating the instrument, the buttons that are not in use are inactivated.

  • Page 17: Setting

    2.3 Settings Generally, users do not need to change the “Settings” of the instrument since they are preset at the time of manufacture. If users need to reset the date, time, or other options, users can adjust or change the options/parameters from the “Settings” menu described below. 2.3.1 Press the “Settings”...

  • Page 18: Setting The Date And Time

    2.4 Setting the Date and Time 2.4.1 On the “Settings” screen, select the date and time buttons. 2.4.2 After selecting the field, erase the number using the “Backspace” button ( ), set the date or time using the number buttons, and press the “Apply” button ( ) to save the changes.
  • Page 19 IMPORTANT Remember that the “Raw Image” MUST be turned on to save the “Raw Image” which should be provided to your distributor or Logos Biosystems to get the best technical support. 2.5.3 The “Auto-header” which is red-lined in the above “Save Options” image is the newly created folder name or file name that all the images start with during the saving process.

  • Page 20: Counting Options

    2.6 Counting Options 2.6.1 In the “Counting Options”, users can adjust Staining Option and Auto Exposure function. <Staining Options> 2.6.2 In the “Staining options” of “Counting Options”, users can choose one of the three staining options as below. Staining Options Description With Trypan...
  • Page 21 <Dilution Factor Check> Note: The setting of the “Dilution Factor” must be checked and done manually. When users choose these 2 options, the “Dilution Factor” at the “Protocol” menu must be checked for correction. The “Dilution Factor” does not automatically change. For example, without a dye, the “Dilution Factor”...
  • Page 22 2.6.3 “Auto Exposure” function of LUNA™ Automated Cell Counter can be used on the software version 3.4 or higher. With only “With Trypan Blue (1:1)” option, auto exposure function can be applied. As a default, “Auto Exposure” button is activated (On). The description of “Auto Exposure”...

  • Page 23: Result/Printer/Calculator Options

    2.7 Result/Printer/Calculator Options 2.7.1 This option shows three different menus, as below. <Result/Printer/Calculator Options> Option Sub-option Description The default value is “off.” When it is turned on, the counting result Result Display box displays only the total cell number, not showing live/dead cells. The default value is “Total Cells (on).”...

  • Page 24: Chapter 3 - Counting Cells

    Chapter 3 – Counting Cells 3.1 Sample Preparation 3.1.1 Prepare the following materials in order to count cells  Cell sample  LUNA™ Cell Counting Slides (Cat #L12001) or LUNA™ Reusable Slide (Cat #12008)  Trypan Blue Stain, 0.4% (Cat #T13001) or Erythrosin B Stain (Cat #L13002) 3.1.2 If desired, insert a USB drive to save data and results, 3.1.3 Mix 10 µl of the cell sample with 10 µl of Trypan Blue Stain or Erythrosin B Stain gently in a microtube by pipetting up and down.
  • Page 25 3.3.1 Insert the sample-loaded slide into the slide port of the instrument, ensuring that the loaded chamber is inserted first into the slide port. The instrument analyzes only the inserted chamber upon counting. When completely inserted, a soft click can be heard. Note: After inserting the slide, LUNA™...
  • Page 26 Note: To get the best focus, the image can be magnified by using the “Zoom-in” button located on the right side of the “Count” button. Initially, the “Zoom-in” button is set to a 1x image. When pressed once, a 2x image appears. This 2x image may be the best zoom-in status for focusing. When pressed again, a 4x image will be shown.

  • Page 27: Using The "Tag" Function

    3.4 Using the “Tag” Function 3.4.1 After performing the counting function, the “Tag” button can be used to verify the counting results. <Tagged Cell Image> Press “Tag” button Green circle – live cell Red circle – dead cell Note: This “Tag” function is one of the distinct tools of the LUNA™ automated cell counter because it allows the user to review the data to determine the accuracy of the counting on-site without depending on a computer.

  • Page 28: Cell Size And Number Distribution

    3.5 Cell Size and Number Distribution 3.5.1 To obtain more data on the distribution of cell size and number in graphical representation, press the “Graph/Gating” button on the lower left side of the screen. A histogram will appear with more details on the size and number of the cell sample as shown below.
  • Page 29 <Combination: Live Cells and Cell Number> The combination of “Live” and “Cell number” shows the distribution of cell size vs. cell number for only live cells. <Combination: Dead Cells and Cell Number> The combination of “Dead” and “Cell number” shows the distribution of cell size vs.
  • Page 30 <Combination: Live Cells and Cell Number> The combination of “Live” and “Cell number” shows the distribution of cell size vs. cell number for only live cells. <Combination: Dead Cells and Cell Number> The combination of “Dead” and “Cell number” shows the distribution of cell size vs.
  • Page 31 <Combination: Total Cells and Concentration> The combination of “Total” vs. “Cell concentration” shows the distribution of cell size vs. cell concentration for total cells. <Combination: Live Cells and Concentration> The combination of “Live” vs. “Cell concentration” shows the distribution of cell size vs. cell concentration for only live cells.
  • Page 32 3.5.1.1 Graphical representation of live and dead cells When the “Total” button icon is clicked, the histogram is expressed as a “Stacked Column Chart.” <Graphical Representation of Live and Dead Cells> Live cells Dead cells 3.5.2 “Cluster Map” button 3.5.2.1 When the “Cluster Map” button is pressed, the percentage distribution of the clustered cells are displayed.

  • Page 33: Using The Dilution Calculator

    3.6 Using the Dilution Calculator 3.6.1 The LUNA™ provides a built-in dilution calculator that helps easily calculate adjustments to obtain a desired concentration. <Built-in Dilution Calculator> 3.6.2 The dilution calculator initially shows the current concentration. Put the appropriate numbers into the blanks of the “Desired Concentration” and “Final Volume” that you want to obtain. Users can choose one of “Total”, “Live”...
  • Page 34 Users can also calculate their custom dilution. 3.6.3 Press the “Calculate” button and then a dilution instruction will appear in the message box. www.logosbio.com...

  • Page 35: Saving The Image And Generating A Report

    3.7 Saving the Image and Generating a Report of the Current Count 3.7.1 To save data and/or generate a report, insert a USB drive into the USB port on the right side of the instrument. Note: Please note that it takes more time to save all the images and PDF report. 3.7.2 Press the “Save”...
  • Page 36 3.7.3 Enter the file name using the keyboard buttons on the screen. The date can be added by pressing the “Date/Time” button. Note: Make sure that the cursor is located in the file name box before entering the file name. 3.7.4 Once the file name is verified, press the “Enter”...
  • Page 37 <Cell Information File>  The .csv file automatically saved shows more detailed cell information such as each cell size and cell status.  In the .csv file, the information of each cell can be checked by cell id given by x-axis and y- axis.

  • Page 38: Printing The Counting Results

    Note: The LUNA™ Printer and Paper are specially designed for the LUNA™ and must be purchased only from Logos Biosystems or your local distributor. Note: For more information on the LUNA™ Printer, please see the User Manual of the LUNA™ Printer.
  • Page 39 <Print Button> 3.8.4 The counting results and its protocol will be printed out as below. <Printed Results> Note: The “Print” button prints only the latest counting result. www.logosbio.com...
  • Page 40 Note: If the “Protocol” part of the print is not necessary, users can turn off the option in the “Results/Printer/Calculator” Option in the “Settings” menu, as shown below. <Result/Printer/Calculator Options> www.logosbio.com...

  • Page 41: Chapter 4 - Reanalyzing Saved Count Results

    Chapter 4 – Reanalyzing Saved Count Results The LUNA™ Automated Cell Counter provides an easy-to-use re-analysis function using the previous count results saved in the USB drive for further analysis or review. 4.1 Importing Previous Counts 4.1.1 Insert the USB drive containing the images (TIF format) into the USB port of the instrument. 4.1.2 Press the “Review”...

  • Page 42: Previous Count" Button

    4.3 “Previous Count” Button The button “Previous Count” in the “Review” menu shows a new window below it that contains up to 1,000 previous counting results that have been saved in the internal memory of LUNA. <”Previous Count” button> <”Previous Count” window> www.logosbio.com...
  • Page 43 1. “Export to USB (.CSV)” – Users can save the data of all previous counts into a USB memory drive which can be handled in PC software. 2. “Erase all” – All data can be removed from the memory. 3. “Close” – Close this window. www.logosbio.com...

  • Page 44: Setting Up The Protocol For Counting

    4.4 Setting the Protocol for Counting For reanalyzing saved images, the LUNA™ Automated Cell Counter enables you to apply protocols containing new parameter sets. Users can have unique protocols for each and apply them to specific cell types. When the “Protocol” button is pressed on the main screen, a list of protocols will show up in the protocol list box.
  • Page 45 Parameter Range Default Description The default value for the dilution factor is “2”. Users can modify this value according to the dilution of the original samples. If diluted for “A” times, users set this variable to “2 x A”. For example, 10 times diluted samples should have the dilution factor of 20 (2 x 10).
  • Page 46 Minimum Use this parameter to set the minimum cell size for Cell Size 3-60 inclusion. The unit is 1 micrometer. (μm) Maximum Use this parameter to set the maximum cell size for Cell Size 3-60 inclusion. The unit is 1 micrometer. (μm) None, Low, There are 4 options for the level of declustering.

  • Page 47: Chapter 5 - Maintenance And Troubleshooting

    Chapter 5 – Maintenance and Troubleshooting 5.1 Cleaning Generally, the LUNA™ Automated Cell Counter does not require regular maintenance for appropriate operation. However, if the instrument is used for long periods of time and continuously, it may need to be cleaned or decontaminated to remove any dirt or dust on the surface of the LUNA™ cell counter. Be sure to turn off the LUNA™...

  • Page 48: Changing The Battery

    5.2 Changing the Battery The battery of the LUNA™ Automated Cell Counter is expected to last for 10 years. However, if the date/time changes or slows with unknown causes, it may indicate that the battery is weakening or running out. To change the battery, contact the service personnel of the instrument supplier in your country.

  • Page 49: Calibrating The Counter

    5.3 Calibrating the Counter In general, recalibration of the LUNA™ Automated Cell Counter is not necessary since it is pre- calibrated during manufacture. However, if needed, please follow the procedure below. 5.3.1 Turn on the instrument to show the Start-Up screen on the display. If the instrument is already turned on, please turn off and on, and then go to the “Start-Up”...
  • Page 50 5) The LUNA™ will show you the progress bar as shown below. Do not turn off the LUNA. <Calibration – Step 1> 5.3.3 Step 2 Calibration 1) After Calibration Step 1, a new pop-up box will come on the screen as shown below. <Calibration –...
  • Page 51 4) Then, LUNA™ will show you the progress bar as shown below. Do not turn off the LUNA. <Calibration – Step 2> 5.3.4 Step 3 Calibration 1) After Calibration Step 2, a new pop-up box will come on the screen as shown below. <Calibration –...
  • Page 52 4) Then press the “Start” button. 5) Then you will see the progress bar as below for custom staining. <Calibration – Step 3> 6) After Calibration Step 3, you will see the message box as shown below. <Calibration Completion> www.logosbio.com...

  • Page 53: Updating The Firmware

    5.4 Updating Firmware 5.4.1 If the instrument is already turned on, please turn off and on, and then go to the “Start-Up” screen. Press the “Settings” button and then the information of the last update and current firmware version will be shown. 5.4.2 Check the firmware version and download the latest version from the LUNA™...

  • Page 54: Troubleshooting

    5.5 Troubleshooting Problem Possible Cause Solution Ensure that the cell image is on an appropriate focus by using the focus knob. You may use the “Zoom-in” button when adjusting the cell Unfocused cell image image. Make sure that live cells have bright centers and dead cells have dark/blue centers.
  • Page 55 Generally, re-calibration takes several minutes. However, it may take more time, depending on the extent of background adjustment. If the calibration takes more than Freezing during calibration 10 minutes, reset the system by turning off and on using the power button located rear side of instrument.

  • Page 56: Chapter 6 - Ordering Information

    Chapter 6 - Ordering Information The following products can be ordered from your regional supplier or the website (www.logosbio.com). Cat # Product Unit LUNA™ Automated Cell Counter L10001 1 unit LUNA™ Cell Counting Slides, 50 Slides L12001 1 box LUNA™ Cell Counting Slides, 500 Slides L12002 10 boxes LUNA™...

  • Page 57: Chapter 7 - Purchaser Notification

    Logos Biosystems, Inc. (“Company”) will not claim any consideration against the purchaser of infringement of patents owned or controlled by the Company which cover the product based on the manufacture, use or sale...
  • Page 58 without issuing a credit. In no event shall the Company accept any returned instrument (including its components) that might have been used or contaminated in some labs, including but not limited to, HIV or other infectious disease or blood- handling labs. This limited warranty does not cover refund, replacement, and repair incurred by accident, abuse, misuse, neglect, unauthorized repair, or modification of the Instrument.
  • Page 59 Logos Biosystems HEADQUARTERS FL 2 & 3 28 Simindaero 327beon-gil, Dongan-gu Anyang-si, Gyeonggi-do 14055 OUTH OREA Tel: +82 31 478 4185 Fax: +82 31 478 4184 Email: sales@logosbio.com 7700 Little River Turnpike STE 207 Annandale, VA 22003 Tel: +1 703 622 4660...

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