Analytik Jena SPEKOL 1300 Manual
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Analytik Jena SPEKOL 1300 Manual

Uv vis spectrophotometer
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SPEKOL
1300
UV VIS Spectrophotometer

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Summary of Contents for Analytik Jena SPEKOL 1300

  • Page 1 ® SPEKOL 1300 UV VIS Spectrophotometer...
  • Page 2 + 49 (3641) 77-7407 Fax: + 49 (3641) 77-7449 e-mail: service@analytik-jena.de General information about Analytik Jena AG on the internet: http://www.analytik-jena.de Copyrights and Trademarks Microsoft, Windows 2000 / XP / Vista, MS Excel are registered trademarks of Microsoft Corp. Publication No.: 210:302.23 Edition –...

  • Page 3: Table Of Contents

    Extension of sample compartment ..............18 Flow-through unit....................19 Thermostatted cell holder................19 Thermostatted 3 cell manual changer ............19 Printer......................20 Display and Buttons of SPEKOL 1300 ......... 21 Software function..............23 Special function ....................23 Select Language Mode ..................24 UV / VIS lamp on / off..................24 Absorbance / Transmittance Measurement............24...
  • Page 4 Determination of the concentration via one point calibration ......25 9.5.2 Determination of the concentration via factor ..........25 9.5.3 Determination of the concentration via calibration curve ........26 DNA / Protein Analytics ..................28 Time Scan ......................29 Disposal .................. 31 ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 5: Introduction

    If some parts are missing, damaged or have any other defects, please contact you dealer or the sales representative immediately. Warning and safety labels on SPEKOL 1300 ® The following warning and safety labels are located on the SPEKOL 1300: Caution! Hazardous area.

  • Page 6: Notes On The User's Manual

    Chapters and illustrations are numbered consecutively. • Every illustration has its own caption. • Steps of operation are numbered. • Cross-references are marked by an arrow (e.g. →Notes on the User's Manual p.3) ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 7: Safety Notes

    Before opening the device or removing any device covers, disconnect the power cable! ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...
  • Page 8 Avoid exposing the sample compartment to strongly corrosive substances! For the analysis of such samples, use vapor-tight stoppered cells. After the measurement, do not leave the samples unnecessarily long in the sample compartment of the device! ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 9: Technical Data

    Temperature working range 15°C – 35°C Humidity working range 85 % 465 mm (18.3’) × 365 mm (14.3’) × 175 mm (6.9’) Size (W x D x H) Weight 11.5 kg ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...
  • Page 10 Technical data ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 11: Design Of The Spekol 1300

    Design of the SPEKOL 1300 Design of the SPEKOL 1300 Display and Button display of data’s and control buttons for use of the internal software function Sample compartment to insert accessories and sample cells Cell selector arm of the Cell changer to change the position of the manual cell changer ®...
  • Page 12 Design of the SPEKOL 1300 Power cable socket Printer power connection power cable socket from the printer Fuse holder Voltage selection to select the voltage between 220 and 110 V Power switch ® Fig. 3 SPEKOL 1300 back side ®...

  • Page 13: Unpacking Instruction

    Unpack the contents carefully and check the materials. Place the instruments in suitable location. ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

  • Page 14: Select Light Source

    [ENT] button to turn on or off the lamp. Press [FUNC] until display shows “UV lamp on?” ( “UV lamp off?” ), then press the [ENT] button to turn on or off the lamps. ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 15: Maintenance

    Re-insert fuse holder and lock the bayonet connection. ® Reconnect the power cable to the power input connector of the SPEKOL 1300 and the power outlet. ® Switch the SPEKOL 1300 on again. ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

  • Page 16: Replacing Halogen Lamp

    Turn the power off and unplug the instrument. Put all samples and not fixed elements out of the sample compartment. Place the SPEKOL 1300 carefully on a soft underlay on the top side of the device so that you can access the underside.
  • Page 17 Replace and fix the holder with the new halogen lamp in the device Close the cover of the lamp house using the 2 screws. 10. Replace the device with the top side on top. ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

  • Page 18: Replacing Deuterium Lamp

    Turn off the power and unplug the instrument Put all samples and not fixed elements out of the sample compartment Place the SPEKOL 1300 carefully on a soft underlay on the top side of the device so that you can access the underside...

  • Page 19: Accessories

    1 to 4 mm. This requires accurate alignment of the cell in the light beam. This adjustable cell holder can be used for cells with an aperture high of 8,5 mm. ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

  • Page 20: Round Cell Holder

    This extension is an attachment for tube openings that allows the use of thermostatted ® cell holders. It can easily be adapted to the SPEKOL 1500 sample compartment. ® Fig. 10 SPEKOL 1500 extension of sample compartment ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 21: Flow-Through Unit

    Use the cell selector arm (→ Fig. 1, p.9) of the manual cell changer to set the position of the manual cell changer. Position 4 Sample 2 Position 3 Sample 1 Position 2 Position 1 Reference Fig. 11 Position of 3 cell changer ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

  • Page 22: Printer

    RS 232 at the right side (→Fig. 2, 4 p.9) and the printer power connection at the back side of the device (→Fig. 3, 6 p.10). Start the instrument new. ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 23: Display And Buttons Of Spekol 1300

    Display and Buttons of SPEKOL 1300 Display and Buttons of SPEKOL 1300 Display and buttons Fig. 12 Digital readout: Display the data of transmittance, absorbance and Display concentration. WAVELENGTH SELECTION DOWN and UP button: Change of [∨] , [∧] wavelengths / Selection of digits when changing numerical datas (factor of concentration analysis) / light source exchange selection / Lamp ON/OFF etc.
  • Page 24 Display and Buttons of SPEKOL 1300 ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 25: Software Function

    Measurement of more than one standard to create a Create standard curve calibration curve Load a calibration curve Load standard curve Delete a calibration curve Delete standard curve Time cycle measurement Time scan ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

  • Page 26: Select Language Mode

    For measurements below 325 nm use quartz cells. Glass or plastic cells are only usable above 325 nm. The liquid level must be at least 20 mm in a standard square cell. ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 27: Concentration Mode

    Use the [∧] or [∨] button to set the factor on the digital display and confirm with [ENT]. Use the [ ∧] or [∨] button to set the decimal point to the right position, confirm and measure with [ENT]. ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

  • Page 28: Determination Of The Concentration Via Calibration Curve

    12. “Save Parameters?” The calibration parameters are automatically saved under serial numbers. At least, 50 calibration curves can be stored. Press the [ENT] button to save the calibration or press [P/C] to abort. ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...
  • Page 29 Choose the number of the calibration curve with the [∧] or [∨] button and press the [ENT] button. Press the [ENT] button again to delete the calibration or [P/C] to abort. ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

  • Page 30: Dna / Protein Analytics

    [P/C] after readout of the first result. Insert another sample or move the arm of the cell changer to the next sample position and strike [Ent]. 11. If factors need to be changed, please strike [P/C] repeatedly until you get to the settings. ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 31: Time Scan

    13. To print out the data points (absorbance value and time) press the [ENT] button or press [P/C] to abort. 14. Confirm the message “Time Scan Over” with [ENT]. ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...
  • Page 32 Software function Time Scan ® Issue 07-2008 User’s manual SPEKOL 1300 Analytik Jena AG...

  • Page 33: Disposal

    (samples) cropping up in the analyses in accordance with the relevant legal and local regulations. When the service life of the device is over, dispose of the device with its electronic components as electronic scrap observing the relevant regulations. ® User’s manual SPEKOL 1300 Issue 07-2008 Analytik Jena AG...

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