Install Consumable Components; Calibrate The System - Thermo Scientific Dionex Operator's Manual

Dionex Integral Systems Operator's Manual
7.2.4

Install Consumable Components

Install the columns, suppressor (if used), and any other consumable
components according to the product manuals.
7.2.5

Calibrate the System

Pump Calibration
Always calibrate the dilution pump and stepper motor loading pump after
replacing any component in the pump flow path that changes the system
backpressure, including columns, sample loops, and tubing. The purpose
of the pump calibration is to accurately determine the pump stroke
volume, using the same hardware components (tubing, fittings, columns,
and so on) used for routine analysis.
1. Open the SP ePanel in Chromeleon 7.
2. Click the button for the procedure to be performed (for example, click
Dilution Pump Calibration to calibrate the dilution pump). A
window appears.
3. Follow the instructions in the window to complete the calibration.
NOTE The peristaltic loading pump, if installed, does not
Standard Loop Calibration
Before running the initial analysis, calibrate the fixed-volume standard
(sample) loop on the metering (ME) valve. Calibration of the standard
loop (and the dilution pump) will determine the correct calibration
standard concentration and ensure accurate analytical results.
Standard loops are usually made from 0.5-mm (0.020-in) ID PEEK tubing
with 10-32 ferrule fittings.
•
For analyses in the ppm to ppb concentration range, use a loop with a
volume of 20 to 100
•
For analyses of 10 to 100 ppb, use a loop with a volume of 100 to
250
88
require calibration. Adjusting the speed of the
pump delivers the required flow rate (see
Section 10.9).
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Doc. 065250-06 03/2020