Zeiss ApoTome.2 Operation Manual
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Zeiss ApoTome.2 Operation Manual

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  • Page 2 Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model. Issued by: Carl Zeiss Microscopy GmbH Carl-Zeiss-Promenade 10 07745 Jena, Germany microscopy@zeiss.com www.zeiss.com/microscopy...

  • Page 3: Table Of Contents

    2.3.3 ApoTome.2 for Axio Zoom.V16 ..................14 General view of ApoTome.2 ..................... 15 Operating principle ......................16 Suitability of objectives for the use of ApoTome.2 ............. 17 Technical data ........................21 STARTUP ......................... 23 Microscope stand requirements ..................23 Unpacking ApoTome.2 ..................... 23 Connecting the control box ....................
  • Page 4 Carl Zeiss Contents ApoTome.2 APPENDIX ........................31 List of abbreviations ......................31 Index ..........................32 423667-7144-001 05/2012...
  • Page 5 Warning and information labels ..................10 Fig. 2-1 System overview ApoTome.2 for Axio Imager ..............12 Fig. 2-2 System overview ApoTome.2 for Axio Observer and Axiovert 200 ........13 Fig. 2-3 System overview ApoTome.2 for Axio Zoom.V16 ............... 14 Fig. 2-4 Components of ApoTome.2....................

  • Page 6: Introduction

    ApoTome.2 through software, please refer to the Operating Manual of the imaging software AxioVision / ZEN 2011 (blue edition). Prior to the initial start-up of the ApoTome.2, read the notes on instrument safety as well as the chapters Instrument Description (Chapter 2) and Startup (Chapter 3).

  • Page 7: Notes On Device Safety

    Carl Zeiss Notes on device safety The ApoTome.2 was designed, produced and tested in compliance with DIN EN 61010-1 (IEC 61010-1) "Safety requirements for electrical measuring, control and laboratory instruments" and meets the requirements of the EC directives 2006/95/EC "Low Voltage Directive" and 2004/108/EC "Electromagnetic Compatibility".
  • Page 8 Carl Zeiss Microscopy Service to repair the instrument. The ApoTome.2 control box is equipped with an integrated power supply which permits the use of line voltages in the range between 100 and 240 V ±10 %, 50 / 60 Hz, without having to adjust the line voltage on the instrument.
  • Page 9 The instruments may only be operated by trained personnel who must be aware of the possible dangers involved with microscopy and the particular application concerned. The ApoTome.2 is a high-precision instrument that can be impaired in its performance or destroyed when handled improperly.

  • Page 10: Warning And Information Labels

    This forfeits all warranty claims. With the exception of the work specified in this manual, no maintenance or repair of the ApoTome.2 may be undertaken. Repairs may only be performed by Zeiss Service staff or specially authorized personnel.

  • Page 11: Instrument Description

    If an Axiovert 200 is used, the control box can only be connected to the PC by a USB cable. A connection via CAN BUS is not possible! Major features of the ApoTome.2 include: − ApoTome.2 slider for the plane of the luminous-field diaphragm in the reflected light path − ApoTome.2 slider with two click-stop positions: Click-stop position 1: An open passage is provided in the reflected light path.

  • Page 12: System Overview

    INSTRUMENT DESCRIPTION Carl Zeiss System overview ApoTome.2 System overview 2.3.1 ApoTome.2 for Axio Imager Fig. 2-1 System overview ApoTome.2 for Axio Imager 423667-7144-001 05/2012...

  • Page 13: Apotome.2 For Axio Observer And Axiovert 200

    INSTRUMENT DESCRIPTION ApoTome.2 System overview Carl Zeiss 2.3.2 ApoTome.2 for Axio Observer and Axiovert 200 Fig. 2-2 System overview ApoTome.2 for Axio Observer and Axiovert 200 05/2012 423667-7144-001...

  • Page 14: Apotome.2 For Axio Zoom.v16

    INSTRUMENT DESCRIPTION Carl Zeiss System overview ApoTome.2 2.3.3 ApoTome.2 for Axio Zoom.V16 Fig. 2-3 System overview ApoTome.2 for Axio Zoom.V16 423667-7144-001 05/2012...

  • Page 15: General View Of Apotome.2

    INSTRUMENT DESCRIPTION ApoTome.2 General view of ApoTome.2 Carl Zeiss General view of ApoTome.2 Slider ApoTome.2 for Axio Imager Slider ApoTome.2 for Axio Observer and Axiovert 200 Slider ApoTome.2 for Axio Zoom.V16 Control box ApoTome.2 Fig. 2-4 Components of ApoTome.2 05/2012...

  • Page 16: Operating Principle

    Fig. 2-6 Image capture in three defined steps (phases) The ApoTome.2 slider is provided with three different transmission grids, which cover the total magnification range, as indicated in Chapter 2.6. The software automatically recommends the optimal grid to be used. Optionally, another grid can be selected manually (e.g. to adjust the section thickness to the experimental conditions).

  • Page 17: Suitability Of Objectives For The Use Of Apotome.2

    INSTRUMENT DESCRIPTION ApoTome.2 Suitability of objectives for the use of ApoTome.2 Carl Zeiss Suitability of objectives for the use of ApoTome.2 The operating principle described in Chapter 2.5 places specific demands on the correction state of the objectives used. Especially the correction of the longitudinal color aberration must be of high quality. Shifting the grid structure along the optical axis minimizes the residual error.
  • Page 18 INSTRUMENT DESCRIPTION Carl Zeiss Suitability of objectives for the use of ApoTome.2 ApoTome.2 Axio Observer / Axiovert 200 Grid / Section thickness DAPI with DAPI with Objective Immersion @ 490nm [RU/µm] FS 34 FS 49 High grid Middle grid Low grid...
  • Page 19 20, 38, 43. Axio Zoom.V16 allows the user to select also the zoom position, besides the objective. ApoTome.2 may be used with Axio Zoom.V16 with a total magnification of 40x or higher. Further filter sets recommended for ApoTome.2 Order No.
  • Page 20 INSTRUMENT DESCRIPTION Carl Zeiss Suitability of objectives for the use of ApoTome.2 ApoTome.2 Order No. Name Excitation / Color splitter / Emission Fluorophores (examples) BP 575-625 488026-9901-000 FS 26 FT 645 mPlum, Nile Red BP 660-710 BP 470/40 489038-9901-000 FS 38 HE...

  • Page 21: Technical Data

    ApoTome.2 slider for Axio Imager ............approx. 278 mm x 90 mm x 76 mm ApoTome.2 slider for Axio Observer/Axiovert 200 ....... approx. 295 mm x 90 mm x 78 mm ApoTome.2 slider for Axio Zoom.V16 ..........approx. 278 mm x 90 mm x 76 mm ApoTome.2 control box ..............
  • Page 22 Technical data ApoTome.2 Fuses as per IEC 127 ApoTome.2 control box ..................T 2 A/H/250 V, 5x20 mm Grid frequencies Axio Imager slider (transmission grids 1 / 2 / 3) ............. 5 / 9 / 17.5 lp/mm Axio Observer / Axiovert 200 slider (transmission grids 1 / 2 / 3) ....... 10 / 17.5 / 35 lp/mm Axio Zoom.V16 slider (transmission grids 1 / 2 / 3) ............

  • Page 23: Startup

    STARTUP The users can install and start up the ApoTome.2 themselves. On request, however, Zeiss Service will install or convert the ApoTome.2 with costs at the customer's place. Before installing and starting up the device carefully read the Notes on device safety.

  • Page 24: Connecting The Control Box

    Fig. 3-1 Connectors on control box (rear panel) • Plug the connection cable ApoTome.2 to the power connector Slider (3-1/3) at the rear side of the control box. • Connect the mains connector (3-1/4) of the control box to a power socket via the mains cable.

  • Page 25: Operation

    OPERATION ApoTome.2 Control and functional elements of ApoTome.2 Carl Zeiss OPERATION Control and functional elements of ApoTome.2 Click stop position 1 - free passage Click-stop position 2 - transmission grid On/off switch Fig. 4-1 Control and functional elements of ApoTome.2...

  • Page 26: Switching Apotome.2 On/Off

    4.2.1 Switching on • Use the on/off switch (4-1/3) at the front panel of the ApoTome.2 control box to switch the device on. • The power indicator in the switch lights up. Switch on the device approximately 15 minutes before the first image capture in order to allow it to warm up to the necessary operating temperature.

  • Page 27: Care, Maintenance, Troubleshooting And Service

    Clean the optical surfaces parallel to the alignment of the grid exerting a slight pressure onto the surface. Clean the transmission grid with extreme care to avoid the damage of the grid structures! For the use of the ApoTome.2 in humid climatic zones, observe the following instructions: •...

  • Page 28: Maintaining The Device

    5.2.1 Performing checks • Make sure that the available line voltage corresponds to the line voltage values as labeled on the ApoTome.2 control box. • Check power cable and power plug for damage. • In the event of perceptible damages, switch off the instrument and safeguard it against inadvertent use.

  • Page 29: Troubleshooting

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ApoTome.2 Troubleshooting Carl Zeiss Troubleshooting Problem Cause Troubleshooting Device does not respond to any no current Actuate power switch, connect control command power cable to power outlet. no connection to the PC Establish CAN BUS cable connection to the stand or the USB cable connection to the PC.

  • Page 30: Requesting Service

    Requesting service All repairs of mechanical, optical or electronic components inside the instrument and of the electrical components of the ApoTome.2 may only be performed by Carl Zeiss Service staff or specially authorized personnel. To ensure optimum setting and trouble-free function of your microscope over a longer period of time, we recommend that you enter into a service/maintenance agreement with Carl Zeiss.
  • Page 31 APPENDIX ApoTome.2 List of abbreviations Carl Zeiss APPENDIX List of abbreviations Conformité Européenne (Conformity with EC directives) Canadian Standards Association Deutsches Institut für Normung (German Institute for Standardization) European Community European standard Mercury vapor short-arc lamp for fluorescence International Electrotechnical Commission...
  • Page 32 APPENDIX Carl Zeiss Index ApoTome.2 Index Page Ambient conditions ..........................21 Care ..............................27 Cleaning the device ..........................27 Click-stop position 1 ..........................26 Click-stop position 2 ..........................26 Connecting the control box .........................24 Control and functional elements ......................25 Designation ............................11 Device safety ............................7 Dimensions ............................21...
  • Page 33 APPENDIX ApoTome.2 Index Carl Zeiss Startup ............................... 23 Storage .............................. 21 Switching off ............................26 Switching on ............................26 System overview ........................12, 13, 14 Technical data ............................ 21 Tools ..............................29 Transmission grid ..........................16 Troubleshooting ..........................29 Unpacking ............................23 Warning labels ...........................

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