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Summary of Contents for Bioer GeneExplorer Series
- Page 1 Thermal Cycler (GeneExplorer Series) Operation Manual Hangzhou Bioer Technology Co., Ltd.
- Page 2 The document contains patent information under copyright protection. All rights reserved. Without prior written approval from Hangzhou Bioer Technology Co., Ltd., any part of the document shall not be copied, re-produced or translated into other language.
- Page 3 Meanwhile, it may damage the safety standard for design and manufacturing , and expected application scope of the instrument. Hangzhou Bioer Technology Co., Ltd. takes no responsibility for any consequences arising from user’s failure for abiding by the following requirements.
- Page 4 Important Notes GeneExplorer Thermal Cyeler Operation Manual voltage required by the instrument (a deviation of 10% is allowed), and ensure the rated loading of the power socket is no less than the requirement of the instrument. D) Attention for power line Generally, the instrument should use the power line supplied along with the instrument.
- Page 5 Important Notes GeneExplorer Thermal Cyeler Operation Manual In any of the following cases, immediately unplug the power supply from the Attention: socket, and contact the supplier or ask qualified maintenance personnel for treatment: Liquid enter into the instrument; The instrument is exposed to rain or wet by water;...
- Page 6 (or fax) back to the company for filing and warranty service. After the packaging box is opened, please properly keep the packaging box and packing materials which will be used during maintenance. Hangzhou Bioer Technology Co., Ltd. will take no responsibility for instrument damage caused by improper packaging during transport to the maintenance department.
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Page 7: Table Of Contents
Contents Important Notes GeneExplorer Thermal Cyeler Operation Manual Contents Chapter One Introduction ..........................2 Introduction to PCR technology ...................... 2 Examples of PCR’s applications ..................... 2 Features of GeneExplorer thermal cyeler ..................2 Use of GeneExplorer thermal cyeler ....................3 Service life of thermal cyeler ...................... -
Page 8: Chapter One Introduction
Chapter One Introduction Important Notes GeneExplorer Thermal Cyeler Operation Manual Chapter One Introduction The chapter covers introduction to PCR technology and examples of its applications, introduction to PCR thermal cyeler and features of GeneExplorer thermal cyeler. 1 Introduction to PCR technology Polymerase Chain Reaction (PCR in short) is a nucleic acid amplification technology of in-vitro simulation of natural DNA reproduction process. -
Page 9: Use Of Geneexplorer Thermal Cyeler
Chapter One Introduction Important Notes GeneExplorer Thermal Cyeler Operation Manual tubes and effectively prevent reagent evaporation and pollution; USB standard port can be connected to general computers, convenient for software upgrade. 4 Use of GeneExplorer thermal cyeler GeneExplorer thermal cyeler, taking a leading position among similar products, has combined various advanced technologies and can meet requirements of various PCR experiments. -
Page 10: Chapter Two Characteristics
Chapter Two Characteristics Gene Explorer Thermal Cyeler Operation Manual Chapter Two Characteristics The chapter mainly introduces the operation, transport and storage conditions of the instrument, and its basic parameters, performance and functions. 1 Normal operation conditions Ambient temperature: 10C ~ 30C RH: ≤70% Power supply: 220V～... -
Page 11: Software Functions
Chapter Two Characteristics Gene Explorer Thermal Cyeler Operation Manual When the set value of hot lid temperature is lower than the set value of module Attention: temperature, the hot lid temperature displayed on the screen will be higher than the set value of hot lid temperature. -
Page 12: Chapter Three Preparations
Gene Explorer Thermal Cyeler Operation Manual Chapter Three Preparations Chapter Three Preparations The chapter mainly introduces the instrument structure, operation of touch screen, and preparations before turning on the instrument. If the instrument is used for the first time, please be familiar with the contents in the chapter before turning on the machine. -
Page 13: Check Before Turning On The Instrument
Gene Explorer Thermal Cyeler Operation Manual Chapter Three Preparations modules hot lid LCD screen power socket power switch USB port network port 1、 Requirements for fuse: 250V 12A 520. For fuse replacement, product which is Attention: in line with the requirements should be used. -
Page 14: Chapter Four Operation Instructions
Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual Chapter Four Operation Instructions In this chapter, the methods of editing, viewing, revising and deleting amplification files are introduced in detail, as well as the method of running PCR amplification files and setting of instrument system parameters. Warning! After the instrument is turned on, if there is any abnormal sound or display, or fault alarm and prompt presents during instrument self-test, please... - Page 15 Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual Press the icon “New Experiment” to enter into the interface of experiment parameter setting. Press the icon “Open Experiment” to enter into the interface of file list. － －...
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Page 16: Pcr Program Editing
Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual Press the icon “Recently Opened” to view experiment. Press the icon “Settings” to enter into Personal Center and System Settings. During operation, instrument operation status is displayed on the main interface, and click to enter into the interface of operation monitoring. -
Page 17: Section Temperature And Constant Temperature Time
Run mode: Standard Mode and High-speed Mode. Standard Mode running characteristics simulate BIOER TOUCH series. 2.3 Function of loop On the interface of PCR program setting, click “Loop”, and it will prompt interface 1-1, select the starting and ending sections of the loop, click “Determine”... - Page 18 Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual Interface 1-1 － －...
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Page 19: Extended Parameter
Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual 2.4 Extended parameter On the interface of PCR program setting, click “Extended parameter” and it will prompt a box for setting extended temperature, extended time, speed, and gradient and other operations. - Page 20 Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual Click it will display the temperature of each module. － －...
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Page 21: Temperature Control Mode
Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual 3.1 Temperature control mode “Control Mode” represents the default temperature control mode after the system is turn on, and it includes two modes of “Block” and “Tube”. “Block” mode only considers the temperature control of module during temperature ramp. -
Page 22: File Management
Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual 4 File management Click Open Experiment on the main interface to enter into the interface of file management. Click the dropdown list on the upper left to select disk for operation. For example, select USB flash disk to operate on USB flash disk contents/GeneExplorer. - Page 23 Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual Press “Function” to delete or rename user. Select user to export to USB flash disk. Click “Manage File” － －...
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Page 24: System Settings
Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual Press “Function” to delete or rename file. Select file to export to USB flash disk. 5 System settings Click “System Settings” on the main interface to enter the interface of setting which includes two major functions which are System Settings and Personal Center. -
Page 25: Software Upgrade
Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual 5. Instrument part use time 6. Dairy 7. About instruments 8. Tm calculation 9. Buzzer setting 10. Exit: exit system software 6 Software upgrade 6.1 UI program upgrade Copy file to USB flash disk \version\update Enter into System Settings->about instruments, select Firmware upgrade/ For setting menu, please pay attention to the capitalization of letters. -
Page 26: Driver Upgrade
Chapter Four Operation Instructions Gene Explorer Thermal Cyeler Operation Manual 6.2 Driver upgrade File Copy to USB flash disk /GeneExplorer location Enter into System Settings->about instruments, select driver upgrade/ － －... -
Page 27: Chapter Five Fault Analysis And Troubleshooting
Chapter Five Fault Analysis and Troubleshooting Gene Explorer Thermal Cyeler Operation Manual Chapter Five Fault Analysis and Troubleshooting The chapter mainly introduces possible faults of the instrument, cause analysis and troubleshooting. 1 Fault analysis and troubleshooting Fault Cause analysis Troubleshooting Check and connect the Disconnecting power supply power supply... - Page 28 Chapter Five Fault Analysis and Troubleshooting Gene Explorer Thermal Cyeler Operation Manual Temperature control time on file editing interface is set as “--:--”, Set temperature control leading to automatic turning off of time in digital form hot lid Loose connector Damaged heating component in Contact supplier...
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Page 29: Wiring Diagram (The Diagram Is Only For Reference, And Please Understand That No Further Notice Will Be Given For Any Modification)
Appendix 1 Gene Explorer Thermal Cyeler Operation Manual Appendix 1 Wiring Diagram (the diagram is only for reference, and please understand that no further notice will be given for any modification) － －... -
Page 30: Appendix 2 Value And Meaning Of Set Parameters
Appendix 2 Gene Explorer Thermal Cyeler Operation Manual Appendix 2 Value and Meaning of Set Parameters Parameter Meaning Set value range Remark Temperature control Temp(C) 4.0~99.0 point (C) Infinite temperature control --:-- (press “+/-“ key before “:” to Temperature control time and turn off hot lid in Time set) - Page 31 Appendix 2 Gene Explorer Thermal Cyeler Operation Manual Key Sound Key sound (yes/no) Yes/No Buzz sound Run End Sound Yes/No Intermittent buzz (yes/no) Module Module Reagent Reagent 图 1 图 2 － －...
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Page 32: Appendix 3 Product Emc Statement
Gene Explorer Thermal Cycler Operation Manual Appendix 3 Appendix 3 Product EMC Statement Class-I Type-A Equipment If the instrument is used under dry environment, especially under dry environment where artificial materials (artificial fabrics, carpet, etc.) present, electrostatic discharge may be generated which may lead to false conclusion. - Page 33 Gene Explorer Thermal Cycler Operation Manual Appendix 3 Radio frequency GB/T 17626.6 3V,150kHz-80MHz,80%AM transmission applicable A The test is applicable only to equipment potentially sensitive to magnetism. CRT display interruption value is allowed to be higher than 1A/m. B Only applicable to cables longer than 3m. C Not applicable to input port expected to connect to battery or chargeable battery (before recharging, it should be removed or disconnected from the equipment).
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Page 34: Appendix 4 List Of Accessories
Gene Explorer Thermal Cyeler Operation Manual Appendix 4 Appendix 4 List of Accessories Model & Name Unit Remark Specification Power line (with shield 250V10A String Accessory magnetic ring clamp) 250V12A, Φ5× 20 Fuse Piece Accessory WiFi Module Piece Accessory Ethernet Patch Cable Piece Accessory Compression Pad... - Page 35 H a n g z h o u B i o e r Te c h n o l o g y C o . , L t d . Add: 1192 Bin An Rd., Hi-tech (Binjiang District), Hangzhou P.C.: 310053 Tel: +86-571- 87774575（or） 87774303 Fax: +86-571- 87772210 E-mail: overseas@bioer.com.cn www.bioer.com.cn...
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