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Bioer LifePro Manual

Thermal cycler
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The Bioer Co. reserves the right to modify this manual at any time without notice.
Caution:
The manual contains copyright protected and patented material. Without prior written consent from
Hangzhou Bioer Technology Co., Ltd., any part of the manual shall not be duplicated, reproduced or
translated into any other language.
Before initial use of this instrument, please carefully
File No.: BYQ60630000000ESM
File version: July 10, 2009
read this manual!
Version 1.0

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Summary of Contents for Bioer LifePro

  • Page 1 Caution: The manual contains copyright protected and patented material. Without prior written consent from Hangzhou Bioer Technology Co., Ltd., any part of the manual shall not be duplicated, reproduced or translated into any other language. Before initial use of this instrument, please carefully read this manual! File No.: BYQ60630000000ESM...
  • Page 2 Hangzhou Bioer Technology Co., Ltd. shall be irresponsible for the consequence resulted from user’s failure to observe the following requirements.
  • Page 3 After unpacking, the packing box and packing materials shall be well kept for future repair. For the damage of instrument occurring during transporting to the repair department due to improper package, Hangzhou Bioer Technology Co., Ltd. shall bear no liability.

  • Page 4: Table Of Contents

    Chapter I Introduction ........................1 1. Introduction of PCR technology ...................... 1 2. Example of PCR technology application scope ................1 3. Features of LifePro Thermal Cycler....................1 4. Use of LifePro Thermal Cycler......................1 Chapter II Features ........................... 3 1.

  • Page 5: Chapter I Introduction

    Botany/zoology: animal inflectional disease diagnosis, plant pathogen detection and etc. 3. Features of LifePro Thermal Cycler LifePro Thermal Cycler is a PCR instrument with multiple functions and several applications. Below are its features: Use advanced thermoelectric refrigeration technology from ITI of US and thermoelectric refrigeration elements made under quality management of Japan, which are steady and reliable in quality.
  • Page 6 LifePro Thermal Cycler Introduction different PCR experiments and rank top in similar products. LifePro Thermal Cycler is attached with: 1) 0.2 ml standard centrifugal tube 2) Strip of 8 tube 3) Strip of 12 tube 4) Full/half apron plates －...

  • Page 7: Chapter Ii Features

    LifePro Thermal Cycler Features Chapter II Features This chapter introduces the usage, transportation, storage conditions of the instrument, as well as its basic parameters, performance and functions. 1. Normal working conditions Ambient temperature: 10°C ～ 30°C Relative humidity: ≤70% Power supply: 220V～, 50Hz...

  • Page 8: Software Functions

    LifePro Thermal Cycler Features If the hot-lid temperature setting is lower than module temperature setting, the hot-lid Caution: temperature shown on screen is higher than hot-lid setting. 4. Software functions a) Edit the file (including file number, temperature and time of each segment, cycle times setting, file view, revision and deletion, automatic file connection, gradient temperature setting, the maximum paragraph, step and cycle times for editable file are 5, 16 and 99 respectively.

  • Page 9: Chapter Iii Preparations

    LifePro Thermal Cycler Preparations Chapter III Preparations This chapter introduces the structure of instrument, keyboard for operation, function of buttons, and preparations before start. Please get familiar with the contents in this chapter for the first time to use this instrument.

  • Page 10: Keyboard Diagram

    LifePro Thermal Cycler Preparations 2. Keyboard diagram 3. Instruction for buttons Character key: to input characters, press button continuously input letters. Display mode switch key: switch display mode between curve and text during running. switch plus/minus program Plus/minus key: temperature editing and time refining.

  • Page 11: Check Before Start

    LifePro Thermal Cycler Preparations 4. Check before start Check the following before insert the plug and power on the instrument: 1) Whether the voltage of power supply matches the voltage required by instrument. (see Chapter II for requirements of power supply) 2) Make sure the power line pug has inserted in power socket reliably.

  • Page 12: Chapter Iv Instruction For Operation

    Main interface Shown on the upper left of main interface is the status of hot-lid setting. Hotlid=off 2009-05-1 10:05:00 Shown in the middle of main interface is the information such as the Bioer information, BIOER software version, module type, default program, default user, control mode, sample Bioer Technology Co.,Ltd...

  • Page 13: How To Edit Pcr Program

    LifePro Thermal Cycler Instruction for operation Test tube type selection Hotlid=off 2009-05-1 10:05:00 Press to +/- select the test tube type in this interface. Press “Back” to return to main interface. BIOER Press “Next” to access the interface for file running if Control Mode is Block now, Bioer Technology Co.,Ltd...
  • Page 14 LifePro Thermal Cycler Instruction for operation Press “Run” to access the interface for file running when Control Mode is Block; press “Run” to bounce the dialog box below if Control Mode is Tube now: Select Sample Volume: 050μl It is the same with main interface, confirm the password, input the sample type and micro liter amount of sample size.
  • Page 15 LifePro Thermal Cycler Instruction for operation Interface for file saving Press character key in this interface to input Hotlid=off 2009-05-1 10:05:00 file name, press confirmation key to move the cursor forward. Select User and File Name by up/down key. Delete character by left key, move cursor by right key.

  • Page 16: How To Set System Parameters

    LifePro Thermal Cycler Instruction for operation File running interface 2 Hotlid=off 2009-05-1 10:05:00 Press to shift between running interface 1 and running interface 2. Temp 1c 95.0 2c 55.0 3c 72.0 4 04.0 Time 1:00 02:00 01:45 00:00 File Name:PCR1 Now Running •••...

  • Page 17: Gradient Module Temperature Distribution Features

    LifePro Thermal Cycler Instruction for operation b) “Tube” mode considers the temperature rise/fall of reagent that lags behind the module. There is an overshoot when it reaches the set temperature so that the reagent reaches the set temperature faster. At the set time same with “Block” mode, obviously the actual time that reagent reaches set temperature is prolonged.

  • Page 18: How To Input/Output Pcr Program

    LifePro Thermal Cycler Instruction for operation 2. How to input/output PCR program 2.1 Connect to USB flash memory USB connection interface Press “UsbLink” in main interface to access Hotlid=off 2009-05-1 10:05:00 the interface for USB connection. If USB flash memory is inserted, as the figure at left shows: The Instrument has Connected UsbDisk.

  • Page 19: Output File To Usb Flash Memory

    1. USB reads/writes 500 users, each user has 2,000 files. Caution: 2. USB reads/writes bioer file at USB root directory in fixed directory. 3. Each user is the next subfolder under bioer file. 4. Don’t plug USB flash memory in/out quickly. To protect device and USB flash memory from damage, the device will terminate the USB function until next run if USB flash memory is plugged in/out quickly.

  • Page 20: Chapter V Connection To Computer

    Click “Setup.exe” in accompanied disc; install the LifePro computer connection program, which is able to control 30 LifePro instruments on one computer. 2. Online setting LifePro is equipped with embedded online module inside and tools for setting are available. 2.1 Tool software installation Step 1, click and run nead_setup.exe.

  • Page 21: Device Configuration

    User’s Manual of LifePro Thermal Cycler Connection to computer 2.2 Device configuration 2.2.1 Connect the device to computer by network cable (cross, B type connection.) network cable compute device 2.2.2 Run Network Enabler Administrator search button configuration mapping function list －...

  • Page 22: Configuration

    User’s Manual of LifePro Thermal Cycler Connection to computer 2.2.3 Configuration Click the search button, and the tool searches instrument automatically. instrument network number it searched Double click the network number in the list column that it searched, open the property page and select network items.
  • Page 23 User’s Manual of LifePro Thermal Cycler Connection to computer Select TCP Server Mode under Operating Mode. Select TCP Server Mode Click OK to complete IP address setting. Click the COM Mapping in function list. － －...
  • Page 24 User’s Manual of LifePro Thermal Cycler Connection to computer confirm add target device remove target device Click the “add target device” button to add target device. Double click the network number setting property mapping page it searched in list column, and select Advanced Settings, setting as shown below.
  • Page 25 User’s Manual of LifePro Thermal Cycler Connection to computer Select Serial Parameters, setting as shown below. Click OK, click “confirmation”, apply the setting, finally click “remove device” and complete mapping setting. 1. Several devices online can’t use a same IP address.

  • Page 26: Main Interface

    User’s Manual of LifePro Thermal Cycler Connection to computer 3. Main interface 3.1 Tool bar Amend user name Input file Change file code Grads calculate Copy file Output user Paste user New file Link equipment Delet file Save file Equipment setup...
  • Page 27 User’s Manual of LifePro Thermal Cycler Connection to computer View running file Run File (A/B) is shown below the device name when device is running file. Double click Run File (A/B) to see running file contents and device running status.
  • Page 28 User’s Manual of LifePro Thermal Cycler Connection to computer View instrument/device storage status Double click System to view instrument/device storage status. Set system parameters Double click Settings sub-item under System items to set system parameters. － －...

  • Page 29: User Menu

    User’s Manual of LifePro Thermal Cycler Connection to computer 3.3 User menu How to… Create new file Change user name Copy user Paste user Delete user Import user Export user Create a user Select device name sub-item in device list or any user name sub-item in device name sub-item.

  • Page 30: File Menu

    User’s Manual of LifePro Thermal Cycler Connection to computer Copy user Select user name to be copied by device list. Select Copy User menu under User menu. Paste user Select User sub-item in device list or device item in device list.
  • Page 31 User’s Manual of LifePro Thermal Cycler Connection to computer Select Create New File menu under File menu to bounce dialog box. 3 Input file name and password, click OK. Edit file Double click user sub-item in device list, open file list, double click edit file or create new file, access the interface to edit file.
  • Page 32 User’s Manual of LifePro Thermal Cycler Connection to computer Paste file Select User item under device list. File can’t be overlaid if it is already existed and open. Prompt File is only copied and pasted between computer and device. File can’t be overlaid if it is already existed and open.

  • Page 33: Tool Menu

    User’s Manual of LifePro Thermal Cycler Connection to computer Save file After file is edited, click Save File item under File menu to save file. Import file Select user item on local computer. Select Export File menu under File menu.
  • Page 34 User’s Manual of LifePro Thermal Cycler Connection to computer One device can only connect to one computer. Disconnect Select device not connected, click Disconnect menu. Calculate gradient Select gradient menu. Input gradient initial point and gradient value. Select 8*12 (96 holes) as calculation module type.
  • Page 35 User’s Manual of LifePro Thermal Cycler Connection to computer － －...

  • Page 36: Chapter Vi Firmware Upgrading

    LifePro Thermal Cycler Firmware upgrading Chapter VI Firmware upgrading 1. Device configuration Connect device to computer by network cable (cross, B type connection). network cable compute device 2. Way to upgrade instrument software 1) Power on, open instrument, press F1 on instrument to access software upgrading preparation status and find “Prepare to upgrade…”...
  • Page 37 LifePro Thermal Cycler Firmware upgrading Network device number it searched Double click the network number searched on list column, open the property page, and select network. Open the property page, select Operating Mode, and click setting. － －...
  • Page 38 LifePro Thermal Cycler Firmware upgrading Select Real com Mode under Operating Mode choices. Select Real Com Mode Click COM MAPPING in function list. － －...
  • Page 39 LifePro Thermal Cycler Firmware upgrading add target device confirm remove target device Click button for add target device. － －...
  • Page 40 LifePro Thermal Cycler Firmware upgrading Double lick network number setting mapping property page searched in list column, and select Advanced Settings, setting as shown below. Select Serial Parameters, setting as shown below. Click OK, click confirmation, and apply the setting.
  • Page 41 LifePro Thermal Cycler Firmware upgrading 9) Firmware upgrading is finished; 10) Click remove device, remove device mapping; 11) Recover the configuration. Open Network Enabler Administrator, select configuration function. configuration Double click network number searched in list column, open property page, and select Network.
  • Page 42 LifePro Thermal Cycler Firmware upgrading Select TCP Server Mode Click OK, close Network Enabler Administrator. 12) Restart the instrument, access interface for instrument system parameter setting, and set system parameters again. Program version might upgrade without notice. User can visit www.bioer.com.cn...

  • Page 43: Chapter Vii Failure Analysis And Handling

    LifePro Thermal Cycler Failure analysis and handling Chapter VII Failure analysis and handling This chapter introduces the failures the instrument might have, and the way to analyze and handle. 1. Failure analysis and handling Failure Analysis Handling Check power Power is not connected...
  • Page 44 LifePro Thermal Cycler Failure analysis and handling Hot-lid closes automatically since temperature temperature control control time in digital time is set as “--:--“ in form file editing interface Hot plug-in is loose Heating element Contact supplier hot-lid is damaged manufacturer...

  • Page 45: Attachment 1 Wiring Diagram

    LifePro Thermal Cycler Attachment1 Attachment 1 Wiring diagram (This diagram is for reference only and subjected to change without notice.) －41－...

  • Page 46: Attachment 2 Value And Meaning Of Parameter Setting

    LifePro Thermal Cycler Attachment 2 Attachment 2 Value and Meaning of Parameter Setting Scope of setting Parameter Meaning Note value Temperature Temp(C) control point 4.0~99.0 (Celsius) --:-- Temperature control time Temperature (press plus/minus to is infinitely long, close Time control time...
  • Page 47 LifePro Thermal Cycler Attachment 2 Hot-lid off Holid Hot-lid on (°C) 30~110 Key Sound Key tone (yes/no) Yes/No Buzz Prompt tone at Run End Sound running finish Yes/No Continuous buzz (yes/no) module module reagent reagent Fig.1 Fig.2 － －...
  • Page 48 Memorandum...

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