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Bioer GeneQ Thermal Cycler Manual

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Note:
Patent materials are included in this manual. All rights reserved. No part of this manual shall be
photocopied, reproduced, or translated into other languages, without written permission from the
Bioer Co.
Please read this manual carefully before the Thermal Cycler is first
Patent No.：ZL 98 2 44367.6
Standard No.：YZB/ 0189 - 2008
Document Number: BYQ60410000000ESM
Document Version:
The Bioer Co. reserves the right to modify this manual at any time without
notice.
FebruaryAprilJan,
operated !
2009
20114
Version
2.01.68

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Summary of Contents for Bioer GeneQ Thermal Cycler

  • Page 1 Note: The Bioer Co. reserves the right to modify this manual at any time without notice. Patent materials are included in this manual. All rights reserved. No part of this manual shall be photocopied, reproduced, or translated into other languages, without written permission from the Bioer Co.
  • Page 2 Otherwise, the guard provided by the Thermal Cycler is likely to be damaged, the rated safety level to be reduced, and the rated operation conditions to be affected. The Bioer Co. shall not be in any way responsible for the consequences resulted from buyer’s not observing the following requirements.
  • Page 3 4 Protection Degree: IP 20 5 LABELS a) Tablet BIOER TECHNOLOGY CO., LTD. Name: Thermal Cycler Model：TC-**/H(b) Power:～220-240V 200W 50/60Hz Fuse: 250V 2.5A(Φ5×20) Sort: classⅠ IP20 SN: BYQ*****-*** Date: ****.**...
  • Page 4 The Thermal Cycler is also warranted for a period of eighteen months, from date of shipment from the company, to be free from defects in material and workmanship. The Bioer Co.’s obligation under this warranty shall be limited to repair or exchange (at the Bioer Co.’s option) of the Thermal Cycler ―III―...
  • Page 5 The buyer is responsible for freight to the maintenance shop designated by the Bioer Co. on all warranty claims. The buyer is also responsible for the transportation expenses of the freight to the maintenance shop.

  • Page 6: Table Of Contents

    Gene Q Thermal Cycler Contents CONTENTS CHAPTER 1 INTRODUCTION........................1 PCR Technique............................1 PCR Applications...........................1 PCR Thermal Cyclers...........................2 Common PCR Thermal Cyclers......................2 Features of the Gene Q Thermal Cycler...................3 CHAPTER 2 SPECIFICATIONS........................4 Normal Operating Conditions......................4 Transportation and Storage Conditions.....................4 Basic Parameters..........................4 Performance............................5 Software Functions..........................

  • Page 7: Chapter 1 Introduction

    Gene Q Thermal Cycler Chapter 1 Introduction CHAPTER 1 INTRODUCTION This chapter briefly introduces PCR technique and its applications, PCR thermal cyclers, and the characteristics of our Gene Q Thermal Cycler. 1 PCR Technique PCR (Polymerase Chain Reaction), or cell-free molecule cloning, is a technique for amplifying nucleic acids in vitro, emulating natural DNA replication process.

  • Page 8: Pcr Thermal Cyclers

    Gene Q Thermal Cycler Chapter 1 Introduction 3 PCR Thermal Cyclers A PCR thermal cycler is a key device throughout the PCR experiment. Its performance determines the accuracy of the experimental results. Its parameters, such as temperature keeping and temperature ramp rate, play an extremely important role in DNA denaturation, annealing and extension.

  • Page 9: Features Of The Gene Q Thermal Cycler

    Gene Q Thermal Cycler Chapter 1 Introduction c) Thermoelectric Cooling Thermal Cyclers These are the most advanced thermal cyclers by far. A thermoelectric cooling thermal cycler also has a metal block at its center (like a compressor cooling thermal cycler). A thermoelectric module closely attached to the block’s outside bottom accomplishes Block heating and cooling.

  • Page 10: Chapter 2 Specifications

    Gene Q Thermal Cycler Chapter 2 Specifications CHAPTER 2 SPECIFICATIONS This chapter describes the Thermal Cycler’s operation, transportation and storage conditions, basic parameters, performance and functions. 1 Normal Operating Conditions Ambient temperature: 10℃30℃ Relative humidity: ≤70% Power supply: ~220-240V, 50/60Hz ,200W 100-120V~, 50/60Hz, 200W Note: Before power-on, please check if the above operating conditions are...

  • Page 11: Performance

    Date and time (year, month, day, hour, minute, second) display and calibration Protecting the device from further damage and alarming in case of failure Note: The above software functions are listed just as a reference. The Bioer Co. reserves the right to modify the software functions without notice. — 5 —...

  • Page 12: Chapter 3 Preparations

    Gene Q Thermal Cycler Chapter 3 Preparations CHAPTER 3 PREPARATIONS This chapter introduces the Gene Q Thermal Cycler’s mechanical structure, the keyboard and each key’s functions, and some preparations before power-on. 1 Instructure Description ④ RS232 interface ① Heated lid & Block ②...

  • Page 13: Keyboard Diagram

    Gene Q Thermal Cycler Chapter 3 Preparations 2 Keyboard Diagram 3 Key Functions “Digit & Letter”, select the letter pressing succession. “Function”， change “+, -” when editing process temperature and modifying time. “Right/Left”，moving the cursor one position to the left or right when pressing during file parameter setting.

  • Page 14: Inspection Before Power-On

    Gene Q Thermal Cycler Chapter 3 Preparations 4 Inspection before Power-on Before switch the power on, please ensure that: Supply voltage falls within the specified limits (refer to Chapter 2); The plug has been inserted into the power socket reliably; The grounding of the power line is reliable.

  • Page 15: Chapter 4 Operation Guide

    On the left top of the main interface it displays Hotlid=off 2008-11-01 10:05:00 the state of Hot lid. On the central area it displays Bioer Co. BIOER information, software version number, block model, default file, default user, control mode and sample volume.

  • Page 16: How To Edit A Pcr Program

    Hotlid=off 2008-07-28 10:05:00 password. BIOER Enter password Press digtal button to enter password Bioer Technology Co.,Ltd directly. Press “Back” to go back the main Version: 2008-2.0 interface. Press “Accept” to confirm the Default User: BIOER...
  • Page 17 Gene Q Thermal Cycler Chapter 4 Operation Guide Flie List Interface Press “Edit” to edit the file indicated by  Hotlid=off 2008-07-28 10:05:00 the cursor. User File Name Save Time  Press “New File”to edit a new file. BIOER1* PCR1 2008-07-28 ...
  • Page 18 Gene Q Thermal Cycler Chapter 4 Operation Guide The sign “*” at the top of User and File indicates the current running file is default Note： one, which can be modified in the parameter setting interface. File Edit Interface In this interface, you can edit sections and Hotlid=off 2008-11-01 10:05:00 segments of the file.
  • Page 19 Select User and File Name through pop-up key. Click left button to delete characters while User： BIOER clicking right button to move the cursor. Press “Caps” key to shift the capital or small File Name：...

  • Page 20: How To Set System Parameters

    “Stop” or “Start”.  Press “Pause” and it will remind File Name:PCR1 “Pause state now. Press ‘F3’ to Now Running  User: BIOER continue running.” According to the Temp=00.0 Time=00:00 Cycle 01 of 10 reminding information you can select...
  • Page 21 Gene Q Thermal Cycler Chapter 4 Operation Guide (Please refer to the following figure: relation between block temperature and reagent temperature.) Block Reagent “Tube mode” has considered the phenomenon that the cooling & heating rate is a little slower than that of block. There is an overshoot after reaching the setting temperature, which can allow reagent to reach the setting temperature faster.

  • Page 22: Chapter 5 Upgrade Of Software

    Gene Q Thermal Cycler Chapter 5 Upgrade of Software CHAPTER 5 UPGRADE OF SOFTWARE 1 To Install Upgrading Software Choose and click “Setup.exe” in the CD, start up guide procedure, and then press “Next ” to continue installation (refer to the picture below). Please confirm “License Agreement”...
  • Page 23 Gene Q Thermal Cycler Chapter 5 Upgrade of Software Please input your information (refer to the following picture), and press “Next” to enter into the installation interface. Please select target installation folder (refer to the following picture), and then press “Next” to enter into the installation interface.

  • Page 24: To Modify, Repair Or Remove The Upgraded Software

    Gene Q Thermal Cycler Chapter 5 Upgrade of Software 2 To Modify, Repair or Remove the Upgraded Software If there is an Gene Q Thermal Cycler installation procedure in your computer, click “setup.exe” to start the guide to modify, repair or remove software (refer to the following picture). You can select “Modify”, “Repair”...

  • Page 25: Preparation Before Upgrading

    Gene Q Thermal Cycler Chapter 5 Upgrade of Software 3 Preparation before Upgrading Install upgrading software of Gene Q Thermal Cycler into your computer. Copy the latest upgrading procedure (*.bin) into your computer. Connect the interface of computer ,“COM1” with the interface of instrument, RS232. Power on the instrument, write down the setting value showed in the system parameter setting interface.
  • Page 26 2) Choose “Advanced” in “Communications Port(COM1) Properties”. Revise the “COM Port Number” in “Advanced Settings for COM1"(as showed in Figure 4). figure 3 figure 4 Note： The user can look through www.bioer.com.cn to download the latest procedure. — 20 —...

  • Page 27: Chapter 6 Failure Analysis And Processing

    Gene Q Thermal Cycler Chapter 6 Failure Analysis and Processing CHAPTER 6 FAILURE ANALYSIS AND PROCESSING In this chapter, we briefly represent possible failures, their analysis and processing. 1 Failure Analysis and Processing Procedures Phenomenon Failure Analysis Troubleshooting Disconnected power Check power supply for correct supply connection...

  • Page 28: Blocks Error Message

    Gene Q Thermal Cycler Chapter 6 Failure Analysis and Processing Failure to press“Save” Please refer to Section 1.2, 2.2 The setup of parameters is invalid button after the setup and 3.2 of Chapter4. of parameters Power off the intrument,upgrade RS232 interface line is the software, and re-connect the loose.

  • Page 29: Appendix 1 Connection Diagram (1/2)

    Gene Q Thermal Cycler Appendix 1 Connection Diagram APPENDIX 1 Connection Diagram (1/2) — 23 —...
  • Page 30 Gene Q Thermal Cycler Appendix 1 Connection Diagram Connection Diagram (2/2) — 24 —...

  • Page 31: Appendix 2 Parameter Value & Meaning

    Gene Q Thermal Cycler Appendix 2 Parameter Value & Meaning APPENDIX 2 Parameter Value & Meaning Item Parameter Meaning Range Note Temp(C) Temp. Control point 4.0~99.0 --:-- The time of Temp. control is （set the value before long limitlessly, meanwhile, the Time of Temp.
  • Page 32 Gene Q Thermal Cycler Appendix 2 Parameter Value & Meaning block block reagent reagent Picture 2 Picture1 — 26 —...
  • Page 33 NOTES...

This manual is also suitable for:

Tc-18/h(b)Tc-24/h(b)

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