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Related Manuals for Bioer GeneExplorer GE-96G
Summary of Contents for Bioer GeneExplorer GE-96G
- Page 1 GeneExplorer Thermal Cycler Instruction for Use GE-96G, GE-384G, GE-4I, GE-48D, GE-48DS, GE-48DG, GE-96S Hangzhou Bioer Technology Co., Ltd. Note: Please understand that no further notice will be given for any...
- Page 2 The document contains patent information under copyright protection. All rights reserved. Without prior written approval from Hangzhou Bioer Technology Co., Ltd., any part of the document shall not be copied, re-produced or translated into other language.
- Page 3 Meanwhile, it may damage the safety standard for design and manufacturing, and expected application scope of the instrument. Hangzhou Bioer Technology Co., Ltd. takes no responsibility for any consequences arising from user’s failure for abiding by the following requirements.
- Page 4 Important Notes GeneExplorer Thermal Cycler Instruction for Use rated loading of the power socket is no less than the requirement of the instrument. D) Attention for power line Generally, the instrument should use the power line supplied along with the instrument. In case of power line damage, it must be replaced instead of repair.
- Page 5 Important Notes GeneExplorer Thermal Cycler Instruction for Use G) Attention for operation During experiment operation, avoid liquid dripping on the instrument. When dealing with reagent, wear personal protective equipment such as protective gloves. Attention: In any of the following cases, immediately unplug the power supply from the socket, and contact the supplier or ask qualified maintenance personnel for treatment: ⚫...
- Page 6 Important Notes GeneExplorer Thermal Cycler Instruction for Use Area with the mark pasted on the DANGER! instrument shall avoid improper use and be careful of danger. Area with the mark pasted on the SCALDING! instrument causes high temperature and is scalding during use.
- Page 7 55 ℃. Instrument calibration Before the instrument is delivered, the device is fully calibrated according to the Bioer 's factory inspection standard, all the actions are under the requirements of ISO 13485 Medical devices - Quality management systems Requirements for regulatory purposes. If the instrument is installed in the required working environment, there is no need to calibrate or correct the temperature after installation.
- Page 8 After the packaging box is opened, please properly keep the packaging box and packing materials which will be used during maintenance. Hangzhou Bioer Technology Co., Ltd. will take no responsibility for instrument damage caused by improper packaging during transport to the maintenance department.
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Page 9: Table Of Contents
Chapter One Introduction GeneExplorer Thermal Cycler Instruction for Use Contents CHAPTER ONE INTRODUCTION ........................3 ......................3 NTRODUCTION TO TECHNOLOGY PCR’ ......................3 XAMPLES OF S APPLICATIONS ..................3 EATURES OF XPLORER HERMAL YCLER ....................4 SE OF XPLORER HERMAL YCLER ...................... - Page 10 Chapter One Introduction GeneExplorer Thermal Cycler Instruction for Use ) ................. 25 FURTHER NOTICE WILL BE GIVEN FOR ANY MODIFICATION ................26 PPENDIX ALUE AND EANING OF ARAMETERS EMC S ......................28 PPENDIX RODUCT TATEMENT ........................30 PPENDIX IST OF CCESSORIES －...
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Page 11: Chapter One Introduction
Chapter One Introduction GeneExplorer Thermal Cycler Instruction for Use Chapter One Introduction The chapter covers introduction to PCR technology and examples of its applications, introduction to PCR Thermal Cycler and features of GeneExplorer Thermal Cycler. Introduction to PCR technology Polymerase Chain Reaction (PCR in short) is a nucleic acid amplification technology of in-vitro simulation of natural DNA reproduction process. -
Page 12: Use Of Geneexplorer Thermal Cycler
Chapter One Introduction GeneExplorer Thermal Cycler Instruction for Use ⚫ Adopting advanced American thermoelectric refrigeration technology thermoelectric refrigeration element manufactured under Japanese quality management mode which presents stable and reliable performance; ⚫ Maximum ramp rate up to 4C/s and can be adjusted based on experiment requirements. ⚫... -
Page 13: Service Life Of Thermal Cycler
Chapter One Introduction GeneExplorer Thermal Cycler Instruction for Use Service life of Thermal Cycler Service life of Thermal Cycler is 5 years from the date of instrument installation. － ―... -
Page 14: Chapter Two Characteristics
Chapter Two Characteristics Gene Explorer Thermal Cyeler Operation Manual Chapter Two Characteristics The chapter mainly introduces the operation, transport and storage conditions of the instrument, and its basic parameters, performance and functions. Normal operation conditions Ambient temperature: 10C ~ 30C RH: ≤70% Power supply: 220V～... -
Page 15: Software Functions
Chapter Two Characteristics Gene Explorer Thermal Cyeler Operation Manual Temperature 4.0-105° C(min. resolution: 0.1° C) range File saving ≥2000 standard programs amount Operation Continuous operation mode Dimensions 420mm× 269mm× 254mm(L× W× H) Net weight 13kg Attention: When the set value of hot lid temperature is lower than the set value of module temperature, the hot lid temperature displayed on the screen will be higher than the set value of hot lid temperature. -
Page 16: Chapter Three Preparations
Gene Explorer Thermal Cyeler Operation Manual Chapter Three Preparations Chapter Three Preparations The chapter mainly introduces the instrument structure, operation of touch screen, and preparations before turning on the instrument. If the instrument is used for the first time, please be familiar with the contents in the chapter before turning on the machine. -
Page 17: Check Before Turning On The Instrument
Gene Explorer Thermal Cyeler Operation Manual Chapter Three Preparations 520. For fuse replacement, Attention: 1、 Requirements for fuse: 250V 12A module hot lid LCD screen pressure balancing compression pad power socket power switch product which is in line with the requirements should be used. Contact the plate hole supplier or manufacturer if you need fuse. -
Page 18: Chapter Four Operation Instructions
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual Chapter Four Operation Instructions In this chapter, the methods of editing, viewing, revising, and deleting PCR amplification files are introduced in detail, as well as the method of running PCR amplification files and setting of instrument system parameters. - Page 19 Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual Picture for GE-96G Picture for GE-48DS Picture of Model GE-384G/GE-4I/GE-96S would show the module type with 384G, plate and 96V respectively. Press the icon “New Experiment” to enter into the interface of experiment parameter setting.
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Page 20: Pcr Program Editing
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual Press the icon “Open Experiment” to enter into the interface of file list. Press the icon “Recently Opened” to view experiment. Press the icon “Settings” to enter Personal Center and System Settings. During operation, instrument operation status is displayed on the main interface, and click to enter the interface of operation monitoring. -
Page 21: Setting Of Program Section
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual Picture for “New Experiment” selection 2.1 Setting of program section Select one section of the program and the section will light up; click “Section”, then there will prompt the checkbox of “Insert” and “Add”. Click “Insert”, a new section will be added before the section selected and lighting up. -
Page 22: Function Of Loop
GeneExplorer Thermal Cycler Operation Manual Run mode: Standard Mode and High-speed Mode. Standard Mode running characteristics simulate BIOER TOUCH series. 2.3 Function of loop On the interface of PCR program setting, click “Loop”, and it will prompt interface 1-1, select the starting and ending sections of the loop, click “Determine”... -
Page 23: Extended Parameter
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual Interface 1-1 Interface 1-2 2.4 Extended parameter On the interface of PCR program setting, click “Extended parameter” and it will prompt a box for setting extended temperature, extended time, speed, and gradient and other operations. Extended temperature: the volume of temperature increase or decrease for each loop. -
Page 24: Save Experiment
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual 2.5 Save experiment On the interface of PCR program setting, click “Save”, and input username and file name to save the file. Files saved can be found under “Open Experiment”. 3 Run program Before running experiment, set up hot lid temperature, reaction volume, temperature control mode and control mode on the interface of PCR program setting and then click “Run”... -
Page 25: Temperature Control Mode
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual Click it will display the temperature of each module. 3.1 Temperature control mode “Control Mode” represents the default temperature control mode after the system is turn on,and it includes two modes of “Block” and “Tube”. “Block”... -
Page 26: File Management
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual the temperature ramp of reagent is lagging that of module, the actual temperature change of reagent is inconsistent with our settings that the actual time of reagent reaching set temperature is shorter than the set time (see the figure below for the relation between module temperature and reagent temperature). - Page 27 Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual New Experiment Click to enter into user management. Press “Function” to delete or rename user. Select user to export to USB flash disk. Click “Manage File” － －...
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Page 28: System Settings
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual Press “Function” to delete or rename file. Select file to export to USB flash disk. 5 System settings Click “System Settings” on the main interface to enter the interface of setting which includes two major functions which are System Settings and Personal Center. -
Page 29: Personal Center
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual 5.1 Personal Center In Personal Center, you can log on with registered account, or log out. 5.2 System Settings System Settings include the following 10 settings: 1. Network setting 2. Http remote access 3. -
Page 30: Driver Upgrade
Chapter Four Operation Instructions GeneExplorer Thermal Cycler Operation Manual 6.2 Driver upgrade File Copy to USB flash disk /GeneExplorer location Enter into System Settings->about instruments, select driver upgrade/ － －... -
Page 31: Chapter Five Fault Analysis And Troubleshooting
Chapter Five Fault Analysis and Troubleshooting Gene Explorer Thermal Cycler Operation Manual Chapter Five Fault Analysis and Troubleshooting The chapter mainly introduces possible faults of the instrument, cause analysis and troubleshooting. 1 Fault analysis and troubleshooting Fault Cause analysis Troubleshooting Check and connect the Disconnecting power supply power supply... - Page 32 Chapter Five Fault Analysis and Troubleshooting Gene Explorer Thermal Cycler Operation Manual Damaged heating component in hot manufacturer Damaged temperature sensor in hot Loose contact of PCB Abnormal characters Contact supplier displayed on screen Damaged PCB manufacturer When setting parameters Hot lid temperature is not set, hot lid for running file, select is set as “OFF”.
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Page 33: Appendix 1 Wiring Diagram
Appendix 1 Gene Explorer Thermal Cycler Operation Manual Appendix 1 Wiring Diagram (the diagram is only for reference, and please understand that no further notice will be given for any modification) － －... -
Page 34: Value And Meaning Of Set Parameters
Appendix 2 Gene Explorer Thermal Cycler Operation Manual Appendix 2 Value and Meaning of Set Parameters Parameter Meaning Set value range Remark Temperature control Temp(C) 4.0~99.0 point (C) Infinite temperature --:-- control time and turn (press “+/-“ key before Temperature control off hot lid in the Time “:”... - Page 35 Appendix 2 Gene Explorer Thermal Cycler Operation Manual In this mode (enter into constant temperature status when overshoot for about 3℃ after Tube reaching temperature), reagent temperature close temperature more quickly (see Fig. 2). Sample Sample volume (ul) 10~200 Volume Hot lid off Holid Hot lid on (℃)
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Page 36: Appendix 3 Product Emc Statement
Gene Explorer Thermal Cycler Operation Manual Appendix 3 Appendix 3 Product EMC Statement Class-I Type-A Equipment If the instrument is used under dry environment, especially under dry environment where artificial materials (artificial fabrics, carpet, etc.) present, electrostatic discharge may be generated which may lead to false conclusion. - Page 37 Gene Explorer Thermal Cycler Operation Manual Appendix 3 frequency applicable transmission Pulse group GB/T 17626.4 1kV(5/50ns.5kHz) applicable signal to Surge GB/T 17626.5 main applicable power Radio 3V,150kHz-80MHz,80%A supply frequency GB/T 17626.6 applicable transmission A The test is applicable only to equipment potentially sensitive to magnetism.
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Page 38: Appendix 4 List Of Accessories
Gene Explorer Thermal Cyeler Operation Manual Appendix 4 Appendix 4 List of Accessories Model & Name Unit Remark Specification Power line (with shield 250V10A String Accessory magnetic ring clamp) 250V12A, Φ5× 20 Fuse Piece Accessory WiFi Module Piece Accessory Ethernet Patch Cable Piece Accessory Compression Pad... - Page 39 H a n g z h o u B i o e r Te c h n o l o g y C o . , L t d . Hangzhou Bioer Technology Co., Ltd. Add: 1192 BinAn Rd, Binjiang District, 310053 Hangzhou,...
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