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Agilent Technologies Genomic DNA ScreenTape System Quick Manual page 4

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Agilent Genomic DNA ScreenTape System Quick Guide
Prepare TapeStation
Parts required
p/n
5067-5365
1
Launch the Agilent 2200 TapeStation software.
2
Load Genomic DNA ScreenTape device and loading tips into the 2200 TapeStation.
Sample Preparation Genomic DNA ScreenTape Assay
Parts required
p/n
5067-5366
1
Equilibrate all reagents to room temperature for 30 min.
2
Vortex mix before use.
3
Prepare ladder by mixing 10 µL Genomic DNA Sample Buffer ( ) with 1 µL Genomic DNA Ladder ( )
Use a fresh ladder for each run. No electronic ladder is available for the Genomic DNA
N O T E
assay.
4
Prepare sample by mixing 10 µL Genomic DNA Sample Buffer ( ) with 1 µL genomic DNA sample
(10 – 100 ng/µL).
5
Spin down, then vortex using IKA vortexer and adaptor at 2000 rpm for 1 min.
6
Spin down to position the sample at the bottom of the tube.
Sample Analysis
1
Load samples into the 2200 TapeStation.
2
Select the required samples on the controller software.
3
Click Start and specify a filename with which to save your results.
Limited Use Label License
Some products within this system contain SYBR® , which is licensed from Molecular Probes Inc. for
use in research and development only. SYBR® is a registered trademark of Molecular Probes, Inc.
Technical Support
For technical support, please visit
Further Information
Visit Agilent Technologies` web site. It offers useful information, support and current developments
about the products and technology:
*G2964-90040*
*G2964-90040*
G2964-90040
Description
Genomic DNA ScreenTape
Description
Genomic DNA Reagents
www.agilent.com/genomics/contact
www.agilent.com/genomics/tapestation
Part Number: G2964-90040 Rev. D
Edition 06/2015
Printed in Germany
© Agilent Technologies, Inc. 2012 - 2014, 2015
Agilent Technologies
Hewlett-Packard-Straße 8
76337 Waldbronn, Germany

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