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General Information on Working with Genomic DNA
• For best results ensure that all reagents are allowed to equilibrate to room temperature
N O T E
• When pipetting Sample Buffer, ensure that excess buffer droplets are removed from the
• When pipetting small volumes ensure that no sample remains within the tip.
• When adding sample buffer to sample or ladder, please ensure that they are mixed
• Briefly centrifuge to collect the contents at the base of the tubes.
• Improper mixing can lead to quantification errors.
Essential Measurement Practices
Environmental
• Optimal operating temperature: 20 °C (68 F)
conditions
• Ambient operating temperature: 15 – 30 °C (59 – 86 F)
Steps before use on
• Equilibrate each vial to room temperature for 30 min.
the TapeStation
• Gently vortex mix each vial and briefly spin.
• 'Flick' ScreenTape device to eliminate bubbles in the separation channel, which could interfere
• Do not shake or over mix ladder vial, this could result in degradation of the Genomic DNA
Steps during sample
• Keep reagents at room temperature during sample preparation.
preparation
Storage after use on
• Store all reagent vials and ScreenTape devices at 2 – 8 °C
the TapeStation
• Never store reagent vials or ScreenTape devices at room temperature or below 0 °C.
• If you run less than 16 lanes, store used ScreenTape device upright at 2 – 8 °C for maximum of 2
Pipette carefully
• Always pipette reagents against the side of the sample tube.
• If using a standard pipette ensure that no residual material is left on the outside of the tip.
Mix properly after
• Mix = Vortex the PCR tubes or 96-well plate using Agilent approved IKA vortexer and adaptor at
each pipetting step
• Spin = Move the samples to the bottom of the tubes/wells by pulsing in a centrifuge.
for 30 min prior to use.
tip before transfer to the sample tubes. Care must be taken due to the viscosity of
Sample Buffer.
correctly. To achieve this, gently mix several times with additional pipetting, then cap the
tubes, vortex mix using IKA vortexer and adaptor at 2000 rpm for 1 min.
with sample loading.
ladder.
weeks.
2000 rpm for 1 min.
Agilent Genomic DNA ScreenTape System Quick Guide
3
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