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3
Optimization of the Agilent 1260 Infinity Quaternary LC VL
Optimizing Detection with DAD
Optimization Overview
Table 10
Optimization Overview
Parameter
1 Selection of flow cell
• Choose flow cell according to used column, see
page 65.
2 Connection of flow cell
• For flow rates from 0.5 ml/min connect column using the zero-dead-volume
fittings of the detector.
• For small column i.d. (e.g 1 mm) the inlet capillary of the micro flow cell can
be connected directly to the column.
3 Setting the peak width (response time)
• Use peak width according
point.
• Set the peak-width close to the width of a narrow peak of interest in your
chromatogram.
4 Setting wavelength and bandwidth
• Sample wavelength:
•
Never miss a peak by the use of a browser wavelength like 250 nm with
100 nm bandwidth.
•
Select specific wavelength with reduced bandwidth if you need
selectivity, e.g. 250,10 nm and 360,100 nm as reference wavelength.
•
Set the sample wavelength to a peak or valley in the spectrum to get
best linearity for high concentrations.
• Reference wavelength:
•
Select the reference wavelength with broad bandwidth (30...100 nm)
wavelength range where your analytes have little or no absorbance (e.g.
sample at 254 nm, reference at 320 nm).
64
"Choosing a Flow Cell"
"Choosing a Flow Cell"
on page 65 as starting
Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
Impact
• peak resolution versus sensitivity
on
• chromatographic resolution
• peak resolution versus sensitivity
versus disk space
• sensitivity versus selectivity
• sensitivity versus linearity
• baseline drift due to RI effects.
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