Image File Settings - GE Amersham Typhoon Operating Instructions Manual

4 Amersham Typhoon Control Software

4.5 Image file settings

4.5

Image file settings

Available image file formats
Depending on the scanning mode, the images can be saved in .gel, .img, or .tif file format.
File
format
.gel
.img
.tif
Description of image file formats
In the fluorescence and phosphor imaging modes, the output log data from the PMT
detectors is first converted to a linear 32-bit floating decimal data format. This data is
stored temporarily for generating 16-bit .img, .gel, and .tif files. Different conversion
functions are used for the different file formats:
•
•
In addition to the .img and .gel formats, the .tif format can be used for presentation of
fluorescence and phosphor imaging data.
In the densitometry mode, the .tif format is used for saving digitization images and relative
OD scans.
76
Fluorescence
General
X
X
X
The .img format uses a logarithmic compression of data which preserves the many
intensity levels of the original data, especially in the low-intensity region. This allows
for measurements over a broad dynamic range. Information about the scan is stored
in a separate text file.
The .gel format uses a square root algorithm to accurately represent small differences
in data, whereas a .tif format may assign the same number to two values, particu-
larly in the fainter part of the image. Image files include information about scan
settings, for example date and time, which can be viewed after the scan, for example
using ImageQuant.
Phosphor
Imaging
DIGE
X X
N/A X
N/A X
Amersham Typhoon Operating Instructions 29193226 AC
Densitometry
OD Digitiz.
N/A N/A
N/A N/A
X X