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NanoEnTek ADAMII User Manual

Bench-top fluorescence cell counter
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Bench-top Fluorescence Cell Counter
User manual
ADAMII™ ©2016 NanoEnTek Inc.
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Summary of Contents for NanoEnTek ADAMII

  • Page 1 Bench-top Fluorescence Cell Counter User manual ADAMII™ ©2016 NanoEnTek Inc.
  • Page 2 They cannot be reproduced, translated, published or distributed without the permission of the copyright owner. Every effort has been made to ensure that all the information contained in the ADAMII™ User Manual is correct at the time of printing. However, NanoEntek Inc. reserves the right to make any changes necessary without notice as part of ongoing product development.

  • Page 3: Table Of Contents

    24-26 · Initial mode 27-33 · Focusing mode - Data menu 34-43 - Setting menu 44-49 Cleaning & Maintenance Troubleshooting 51-52 Error code 53-54 Warranty Safety precautions 56-57 Safety symbols Product specifications Ordering information Technical support ADAMII™ ©2016 NanoEnTek Inc.

  • Page 4: Introduction

    Introduction ADAMII is a bench top fluorescent cell counting analyzer that can be used in either a hospital and research laboratory. It has 4-channel (Bright, Yellow (PE), Red (PerCP) and Green (FITC)) light sources, so three color fluorescence samples can be analyzed simultaneously. The automatic X-Y-Z stage captures a maxi- mum of 136 images and which increases the accuracy of analysis.

  • Page 5: Product Components

    Product components ADAMII™ is shipped with the following components. Once you receive your instrument, please check that all components listed below were shipped. If any component is missing or damaged, contact your local distributor or e-mail ivdst@nanoentek.com Power cord with 4 adaptor cords USB cable ADAMII™...

  • Page 6: Product Description

    Product description Front view Insert/Eject Button Run Button State LED Insert/Eject Insert and eject the slide from the ADAMII™. button Functions as load and unload. Perform all procedures of automatic counting. Run button State LED Light up when software runs in the laptop and connection of camera and serial perform cor- rectly.
  • Page 7 Power Power switch Serial Camera connection connection supply port port port Power supply port Power supply Power switch Power on/off Serial connection port Control the ADAMII™ operating Camera connection port Control the camera of the ADAMII™ ADAMII™ ©2016 NanoEnTek Inc.

  • Page 8: Installation

    3. Place the instrument on a flat, level surface. 1. Plug the power cord into the electrical outlet and connect two USB cables.. Install the instrument Serial Camera Power supply port USB port 2. Connect the laptop and the ADAMII™ using two USB cables.
  • Page 9 Installation Install 1. Turn on the laptop and ADAMII™ by pressing the Power button. At this time, the slide holder will be ejected automatically. SW program 2. Double-click ADAMII™ icon on Desktop to start the software. ☞ Note: The ADAMII™ will be ready to use after initialization is completed when powered on.

  • Page 10: Home Menu

    Home menu Log in At the ‘Home’ screen, enter the user ID and password to log in. Default ID and password are both ‘master’ . User Click ‘User management’ when you need to manage accounts (e.g. ID addition, password change). To create a new ID, fill out the blank (user management name, user ID, password, confirm PW) and click ‘...
  • Page 11 1. Enter the User ID and set the date that you want to search. 2. Click ‘Search’ button. 3. Information of the selected user will appear. ☞ Note: Only the master user can access the ‘ ‘ A udit trail” . ADAMII™ ©2016 NanoEnTek Inc.

  • Page 12: Calibration

    Calibration 1. For calibration, go to ‘Setting’ menu and click ‘Calibrate’ . Calibration procedure 2. Then, calibration window will appear.
  • Page 13 4. Once again, vortex for 4 seconds and pipette for 25 μL immediately. 4 sec. [Vortexing] [Pipetting calibration bead 25 μL] 5. Load 25 μL of calibration beads on the ADAMII™ assay slide. [Load 25 μL calibration bead] ADAMII™ ©2016 NanoEnTek Inc.
  • Page 14 [Attach dry-proof closure tape] Warning: • Calibration bead loading error - Ensure that 25μL of calibration bead is fully loaded on the ADAMII™ assay slide. - The instrument will not detect in low or high loading volumes. CAUTION: • Avoid bubbles as they may negatively affect results.
  • Page 15 8. Insert the slide with calibration beads into the slide port of the instrument and press ‘Insert sample’ button. 9. Enter lot number of calibration beads. 10. Select Bright channel and adjust the focus using the focus knob. ADAMII™ ©2016 NanoEnTek Inc.
  • Page 16 Calibration ☞ Note: Please check ‘ADAMII™ Focus guide’ before setting the focus. Calibration procedure [Focusing example - representative images] Bright- Good Focus Bright- Bad Focus FL- Good Focus FL- Bad Focus • Bright - Good Focus: Clear circumference of the beads, Dark and clear contrast to the background •...
  • Page 17 ☞ Note: Save the focus in ‘Bright’ and ‘PerCP’ channel. The other channels will be automatically focused. 14. Press the ‘Run’ button to start the auto calibration. Calibration will take about 3 minutes to count the 24 frames. 3 min. ADAMII™ ©2016 NanoEnTek Inc.
  • Page 18 Calibration Calibration 15. When the calibration is completed, the results will be displayed. procedure ☞ Note: If the results are not within the range on the tube, please ask your manufacturer or distributor. 16. When you need previous results of calibration, click ‘Record’ button.
  • Page 19 Calibration Calibration 17. Calibration result list records will appear. procedure 18. Click ‘Save’ button to save the calibration records. The result will be saved in CSV format. 19. Press “Close” button to finish calibration. ADAMII™ ©2016 NanoEnTek Inc.

  • Page 20: Sample Loading

    Prepare sample and check the sample type. preparation To run a sample, click ‘Measure’ menu. Load sample Load 25 μL sample into the ADAMII™ assay slide and attach a dry- proof closure tape. [Load 25 μL sample] [Attach dry-proof closure tape] ☞...

  • Page 21: Operation

    Operation Insert 1. Open the ADAMII™ door. assay slide 2. Press to open up the slide holder. 3. Insert the assay slide into the slide holder. ☞ Note: Assay slide should be inserted to the end of the slide holder, in the direction of arrow on the slide, facing up.
  • Page 22 Warning: • Using ADAMII™ assay slide - Do not reuse the ADAMII™ assay slide, as leftover dye from the previous reading may affect the next reading. - Do not use any other slides as it results in inaccurate count and...
  • Page 23 · Slide inserting and ejecting - blinking Warning: • Close the door after inserting the sample. • Do not look into the ADAMII™ instrument when door is open. Direct exposure to high-energy LED light may be harmful to the eyes. ADAMII™ ©2016 NanoEnTek Inc.

  • Page 24: Measure Menu

    Operation To do a sample test, click the ‘Measure’ menu. Measure menu - Initial mode ☞ Note: Above screen and upcoming screen images only relate to CD34 application. 1. Counting mode 2. Display area 3. Detection channel 4. Insert sample 5.
  • Page 25 (Refer to page 28) channel Insert the sample loaded assay slide and click ‘Insert sample’ button ④ Insert sample to start the test. ☞ Note: Please check the door and slide holder cap is closed. ADAMII™ ©2016 NanoEnTek Inc.
  • Page 26 Operation Counting results of the sample will be displayed. It will be activated Measure menu after inserting sample. (Refer to page 33) - initial mode ⑤ Result area Date, sample/patient ID, and operator information will be displayed. ⑥ Test information...

  • Page 27: Focusing Mode

    Once the assay slide is inserted, focusing mode will appear. Measure menu - Focusing mode ☞ Note: Above screen and upcoming screen images only relate to CD34 application. 1. Display area 2. Detection channel 3. Focus option 4. Save focus 5. Run sample 6. Eject ADAMII™ ©2016 NanoEnTek Inc.
  • Page 28 Operation Display preview image of the sample from the camera. Measure menu - Focusing mode ① Display area Indicate which channel is displayed. For ADAMII™-CD34 assay, ② Detection three fluorescent channels correspond to the markers below: channel • PerCP: CD45 •...
  • Page 29 • 2ch manual focus: Set the focuses for two channels ‘Bright’ and ‘PerCP’ manually, then the other two fluorescent channels will be automatically focused, when this option circle is selected. ☞ Note: 2ch manual focus is recommended. [Save BR focus] [Save PerCP focus] ADAMII™ ©2016 NanoEnTek Inc.
  • Page 30 Operation ☞ Note: Please check ‘ADAMII™ Focus guide’ before setting the focus. Measure menu - Focusing mode [Focusing example – bright field] ③ Focus option Bright- Good Focus Bright- Bad Focus #1 Bright- Bad Focus #2 Bright- Bad Focus #3 •...
  • Page 31 Operation ☞ Note: Please check ‘ADAMII™ Focus guide’ before setting the focus. Measure menu - Focusing mode [Focusing example – fluorescence channel] ③ Focus option CD45 PerCP- Good Focus CD45 PerCP- Bad Focus CD34 PE- Good Focus Bright- Bad Focus #2...
  • Page 32 Operation 1. Once the focusing is done, dilution factor and number of frames to Measure menu capture. If you want to see the viability, please check the circle - Focusing mode under the viability circle. ⑤ Run sample 2. Press the “Run sample” button. 3.
  • Page 33 ☞ Note: The door should be closed when the slide holder is being ejected. If not, the door can be damaged. ☞ Note: If the door is opened, the sample will not be ejected. ADAMII™ ©2016 NanoEnTek Inc.

  • Page 34: Data Menu

    Operation Data menu provides the raw data including counting results, images Data menu from each frame in each channel. Users are able to review, save and export the data. Data list ☞ Note: Above screen and upcoming screen images only relate to CD34 application.
  • Page 35 • Sample type: display the sample type (HPC-A, Mobilized PB, Control) • Reading frame/volume: total frames and volume • Deleted frame/volume: deleted frames and volume • Result frame/volume: final result frames and volume (Subtract the deleted frame/volume from the reading frame/volume) ADAMII™ ©2016 NanoEnTek Inc.
  • Page 36 • Purple: cells counted in PerCP channel (CD45 in ADAMII™-CD34 assay) • Green: cells counted in PE channel (CD34 in ADAMII™-CD34 assay) • Red: cells counted in FITC channel (dead cells in ADAMII™-CD34 assay) ☞ Note: This function is available only in the data menu.
  • Page 37 Double click the captured image, then you can check preview images of Data menu selected channel on the right side of the screen. To check the preview im- ③ Captured ages of other channels, select that channel and double click the captured image image again. ADAMII™ ©2016 NanoEnTek Inc.
  • Page 38 ④ Sample volume The default numerical values of total and sample volumes for each sample type in ADAMII™-CD34 assay are shown below: information • HPC-A: total volume = 275 μL, sample volume 20 μL • Mobilized PB: total volume = 60 μL, sample volume 20 μL •...
  • Page 39 Frame by frame revision of raw data image and removal of invalid frames can be done on the table. For deleting an invalid frame, click on the right mouse button and select ‘Delete’ menu. Then, the “Result area” will show the re-calculated data, reflecting the deleted frame(s). ADAMII™ ©2016 NanoEnTek Inc.
  • Page 40 Operation How to find the invalid frames is described below: Data menu ⑦ Raw data table 1) Sample aggregation: when there is aggregation in specific area of sample, that frame should be deleted. Br image FL image 2) Dust: when there is dust inside the slide, that frame should be deleted.
  • Page 41 4) Cell debris: when there is cell debris, that frame should be deleted. Data menu ⑦ Raw data table Br image FL image 5) Overlapped circle: when there are several circles in one cell, that frame should be deleted. Br image FL image ADAMII™ ©2016 NanoEnTek Inc.
  • Page 42 Operation To review the data that is saved in ADAMII™, select the desired sample from Data menu the data list. Scroll up and down to check the data of sample. ⑧ Data list Data list ADAMII™ allows you to export the data table(.csv), images(.jpg), ⑨...
  • Page 43 • Select all: Click ‘Select all’ button to select all of the listed data. You can also select all of the sample data at once by pressing ‘Shift’ button. • Delete: To delete a file, select the data file and click ‘Delete’ button. ADAMII™ ©2016 NanoEnTek Inc.

  • Page 44: Setting Menu

    Operation Setting menu contains information of ADAMII™ as well as several Setting menu user preferences and other Settings. It allows users to edit settings about the basic operation, software and firmware information. 1. Date & Time 2. Counting settings 3. Calibration 4.
  • Page 45 - Decreasing the value from 80% allows objects to be less round - Adjusting this may be useful if the cell type is not particularly circular or perhaps oddly shaped ADAMII™ ©2016 NanoEnTek Inc.
  • Page 46 Operation Please refer to page 12~19 for calibration. Setting menu ③ Calibration To open the calibration result list, click ‘Record’ button. You can check the data of calibration briefly including date, time, lot number, user ID and result of each channel. Select the sample type.
  • Page 47 3. Find the updated software in the USB drive and double click the file to execute. 4. When you see the window below, the update completed successfully. Press OK button to proceed. 5. Program will be restarted automatically. Check that the version of software was modified properly. ADAMII™ ©2016 NanoEnTek Inc.
  • Page 48 Operation [Firmware update] Setting menu 1. Save the firmware on USB disk and connect to the laptop. ⑥ Update 2. Press ‘FW update’ button. 3. Move to the folder where ‘Firmware update file’ is located and click the file. 4. Press OK button when the automatic update is completed. 5.
  • Page 49 ⑦ Device info tion status if ‘Disconnected’ is displayed. Disk info indicates available hard disk space. Please back up or ⑧ Disk info delete your data from the hard drive when the free space is less than 100MB. ADAMII™ ©2016 NanoEnTek Inc.

  • Page 50: Cleaning & Maintenance

    Cleaning and Maintenance Clean the surface of ADAMII™ instrument with a damp cloth. If liquid spills on ADAMII™, turn off the power immediately and wipe dry. ADAMII™ does not need regular maintenance. To troubleshoot problems with ADAMII™, contact technical support.

  • Page 51: Troubleshooting

    • Set the focus with 2ch manual focus function with autofocus function • First, check the sample type • Second, if you used the bottom of ADAMII™ CD34 Reagent Background of image is that almost used, please use the new reagent suddenly brighten •...
  • Page 52 • If none of the above 3 steps solve the problem, please request technical support • ADAMII™ does not insert and count the slide when the slide holder cap opened. Sensor of slide • But if sensor of slide holder cap is faulty and you start counting without holder cap is faulty closing the cap, then the result will not be accurate.

  • Page 53: Error Code

    Error code Reason Message Position Camera E0001 connection fail Driver Serial E0002 connection fail Serial Stage E0010 disconnected working Camera E0020 Camera disconnected. Open the slide Slide E0030 holder inserting Open the slide Slide E0031 door ejecting ADAMII™ ©2016 NanoEnTek Inc.
  • Page 54 Error code Error code Reason Message Position Data storage E0040 Data save is full Sample density Measuring E0050 is too high E0060 Update fail SW update...

  • Page 55: Warranty

    Warranty NanoEnTek provides 1-year warranty service for defects of material and workmanship. If any defects occur in ADAMII™, NanoEnTek provides repair services for the defective parts at its discretion. The following defects, however, are specifically excluded: 1. Defects caused by improper operation.

  • Page 56: Safety Precautions

    Safety precautions Review and follow the safety instructions below: • If water or other material enters the instrument, adaptor, or power inlet, disconnect the power cord and contact a service person. For operating environment, refer to Product Specifications. • Do not touch the main plug or power cord with wet hands. •...
  • Page 57 Dans la documentation de l’utilisateur, la declaration doit etre incluse pour attirer l’attention sur le fait qu’il peut y avoir des difficultes potentielles pour assurer la compatibilite electromagnetique dans d’autres environnements, en raison des perturbations rayonnees et. ADAMII™ ©2016 NanoEnTek Inc.

  • Page 58: Safety Symbols

    4. For more detailed information about disposal of your old appliance, please contact your city office, waste disposal service or visit our web-site, www.nanoentek.com. This product conforms to UL 61010-1, CAN/CSA C22.2 No.61010-1 “Safety Requirements for Electrical Equipment for Measurement, Con- trol, and Laboratory Use, Part 1: General Requirements.

  • Page 59: Product Specifications

    • CPU: 6th Generation Intel® Core™ i3-6100U Processor   • OS: Windows 8.1 (64 bit)  • RAM: 4 GB DDR3L 1600MHz • HDD: 500GB 7200rpm Hard Drive  • 14” Full HD (1920 X 1080) Anti Glare(16:9) WLED ADAMII™ ©2016 NanoEnTek Inc.

  • Page 60: Ordering Information

    Ordering information Cat. No. Description Contents · Main instrument ADAMII , Bench-top ADAM2 · Power cord Fluorescence Cell Counter · USB connection cable · CPU : 6th Generation Intel® Core™ i3-6100U Processor   · OS : Windows 8.1 (64 bit) ...

  • Page 61: Technical Support

    Technical support Visit the our Website at www.nanoentek.com for : · Technical resources, including manuals, FAQs, etc. · Technical support contact information · Additional product information and special offers For more information or technical assistance, please call or email. Email ivdst@nanoentek.com...
  • Page 62 Memo...
  • Page 63 Memo ADAMII™ ©2016 NanoEnTek Inc.
  • Page 64 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel : +82-2-6220-7942, Fax : +82-2-6220-7999 MT Promedt Consulting GmbH Altenhofstrasse 80, 66386 St. Ingbert, Germany US Branch NanoEnTek USA Inc. 5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA Tel.: +1-925-225-0108, +1-888-988-0108(Toll free), Fax.: +1-925-225-0109...

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