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Summary of Contents for NanoEnTek ADAM MC Plus
- Page 2 All the materials in this user manual are protected by Korean and international copyright laws. They cannot be reproduced, translated, published or distributed without the permission of the copyright owner. ADAM MC Plus Instruction Manual Website: www.nanoentek.com E-mail: sales@nanoentek.com Manufactured by NanoEntek, Inc.
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Page 3: Table Of Contents
Table of Contents Introduction ........................... 5 General Description......................5 Technology ........................6 Basic principle of counting ....................7 Product contents ........................8 ADAM MC Plus ......................8 AccuPlus Slide ......................... 8 Upon receiving the instrument ..................8 Product Description ......................9 Front view of ADAM MC Plus .................. - Page 4 Update .......................... 28 Power off ..........................29 Lock ..........................29 Power off ........................29 Maintenance and cleaning ....................30 Trouble shooting ........................ 31 Warranty ..........................32 Technical specifications ....................33 Product list ......................... 34 Safety precautions ......................35 Mesures de sécurité ......................36 Safety symbols ........................
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Page 5: Introduction
Introduction The ADAM MC Plus is a new standard of highly accurate General automated cell counter equipped with bright field and two Description fluorescence channels (AO/DAPI). ADAM MC Plus... -
Page 6: Technology
Also, Trypan Blue exclusion method has been known to overestimate cellular viability. In addition to these limitations, manual counting can be user-dependent. To address these problems, NanoEntek has developed ADAM MC Plus. ADAM MC Plus is an image-based fluorescence cell counter. It takes bright field image and two color fluorescence images. -
Page 7: Basic Principle Of Counting
Introduction MC Plus uses Acridine Orange (AO) and 4’,6-diamidino- ADAM Basic 2-phenylindole (DAPI) to count total number of cells and number of principle of dead cells, respectively. AO is a cell-membrane permeable dye that counting stains nucleus of every cells regardless of the cell’s condition. Therefore, it is used to count total number of cells. -
Page 8: Product Contents
Product contents ADAM MC Plus is shipped in a carton box containing followings; ADAM MC Plus Item Quantity Main device User manual USB hub Wifi dongle Power cord Adapter Printer (optional) AccuPlus Slide has following components: AccuPlus Slide AccuPlus Slide Cell viability reagent Item (Cat. -
Page 9: Product Description
Product Description The front view showing various parts of the ADAM MC Plus is Front view shown below: of ADAM MC Plus Control buttons Description ① Door Slide holder is inserted and ejected. ② Power Power on / off. ③ LCD Display processes and results. -
Page 10: Rear View Of Adam Tm Mc Plus
Product Description The rear view showing various parts of the ADAM MC Plus.: Rear view of ADAM MC Plus Port Description ① Port for software update and save the data. USB Port ② Connects with PC. PC port ③ Connects ADAM MC Plus power cord to wall outlet. -
Page 11: Getting Started
2. Press the power button for 2~3 seconds. Initial Display If you get an error message, please contact your local distributor or sales@nanoentek.com. If booting is successful and no errors are detected, the home screens will be displayed as below. -
Page 12: Error Messages During Booting
It appears when booting is not working properly. Turn off main power and restart device. If this message still appears after restarting, contact your local distributor or sales@nanoentek.com. Error code Cause Failure of X-axis sensor 0x00000C00 0x00007000... -
Page 13: Count Setting
Getting started Count [Cell size] setting Set the minimum and maximum size of cell. Cell size [Dilution factor] When diluting sample, set the Dilution factor. CAUTION Factor values for the Cell viability reagent is already applied. Dilution factor ADAM MC Plus... -
Page 14: General Operation
General Operation Instruction is provided in this section for preparing the sample with Instruction Cell viability reagent for use with disposable AccuPlus Slide for automated cell count using the ADAM MC Plus. Please check the procedure of sample preparation and testing below. -
Page 15: Sample Preparation
General Operation Sample 1. Cultivate the required number of cells. preparation NOTE Concentration out of this range will result in errors. Refer to page 20 for more information about errors. 2. Thoroughly mix the cell pellet by vortexing or pipetting. 3. - Page 16 General Operation Cell WARNING counting [Sample loading error] Be cautious of loading the correct volume of the sample into AccuPlus Slide. The instrument will not detect low or high sample volumes. Correct volume Low volume CAUTION Avoid bubbles which may negatively affect the result. ADAM MC Plus...
- Page 17 General Operation WARNING Cell [AccuPlus Slide insert error] counting Completely insert AccuPlus Slide face up, in the direction of the arrow on the slide. The instrument will not detect if slides are inserted incorrectly. See pictures below for proper insertion. WARNING •...
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Page 18: Measure
Measure Start counting process by pressing ‘START’. Run sample It may take about 2 minutes longer for auto focus at the initial test. While the test is in progress, you can check the cell images of each channel. ADAM MC Plus... -
Page 19: Result Analysis
Measure The result will be displayed after being automatically calculated by Result ADAM MC Plus software. analysis Number of Number of Title Viability Non-Viable cell Total cell Viability 01 1.10E6 5.50E5 Viability 02 2.20E6 5.50E5 • The viability will be automatically calculated by the ADAM Plus software after each measurement of the total cells and the non-viable cells. -
Page 20: Result Analysis - Error Code
Measure Result Analysis - Error code Error code Cause Frames with errors are over 50% of total counting frame. Cells are more than 2 × 10 cells/mL. Cells are more than 1 × 10 cells/mL. Cells are less than 4 × 10 cells/mL. -
Page 21: Data
Data Data list Control buttons Description Select all data in Data List. ① Display the data of the selected date. ② SEARCH View and edit the data. ③ EDIT Multiple data can be edited with the same settings. Check the cell images of each channel. ④... -
Page 22: Edit
Data EDIT Control buttons Description Edit the sample name. ① Sample Allows to view the cell size graph for each channel. ② Cell size graph Allows to view the number of cells in each cell size. T=Total cell (AO), D=Dead cell (DAPI), R=Real cell ③... -
Page 23: Image
Data IMAGE Control buttons Description ① Select images Select images measured in bright field, AO or DAPI. ② Original Check the original image. ③ Counted Check the counted cell image. ④ Frame Select a frame number of the channel. Zoom-in/out Zoom in and out to check the cell image. -
Page 24: Save
Data SAVE Control buttons Description Selects save path from the list to send the selected ① Select Path data. ② Data Type Selects which data type to save. Exports the files to a selected save path ③ Apply Files can be sent to only one save path at a time. ADAM MC Plus... -
Page 25: Mail
Data MAIL Control buttons Description Selects e-mail address from the list to send data. The e-mail address where data has been sent will ① History be saved. To send files to new e-mail, enter the applicable e-mail ② Mail Address address. -
Page 26: Setting
Setting Setting Control buttons Description Set default values of cell size and dilution ① Count setting factor. ② Wifi Set the wifi to use the e-mail function. ③ Remote support Connects to remote support software. ④ Mail Do not change the setting in mail. ⑤... -
Page 27: Wifi
If connection error occurs, please contact a laboratory facility manager Remote support 1. Connect to wifi. 2. Click ‘Remote support’ button. 3. Share your ID and password to NanoEntek. NOTE The remote support feature is to be used for maintenance only by request of NanoEntek. WARNING If you do not see your Remote Support ID and Password, click the ‘Close’... -
Page 28: Update
Setting 1. Prepare the USB with update file. Update 2. Insert the USB. 3. Click the UPDATE button. CAUTION The ‘AdamUpdate’ folder must exist in the root path of the USB folder. • • ADAM MC Plus can be updated only when the firmware or software file Exists in the ‘AdamUpdate’... -
Page 29: Power Off
Power off Press LOCK before turning off the device. Lock If there is no operation for 1 minute, the lock function will be activated automatically. When the device is locked, the screen will be changed as shown below. Power off If you press the power button 2~3 seconds, then ‘Slide to shut down your PC’... -
Page 30: Maintenance And Cleaning
Maintenance and cleaning 1. ADAM MC Plus does not need regular maintenance. Result 2. ADAM MC Plus has no replacement of consumable materials. analysis 3. Please clean the exposed surface of ADAM MC Plus frequently or before testing, using a soft cloth and isopropyl alcohol or deionized water. -
Page 31: Trouble Shooting
Trouble shooting Trouble Problem Description Solution ADAM shooting • No power from outlet • Check power source. Plus does • Bad power cord. • Replace. not power up Inaccurate result • Cell number may be • Adjust the number of cells to recommended out of range. -
Page 32: Warranty
Warranty Warranty If any defects occur in the ADAM MC Plus during one (1) year warranty period, NanoEntek will repair or replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded: 1. Defects caused by improper operation. -
Page 33: Technical Specifications
Technical specifications ADAM MC Plus Technical Measuring range 5x10 ~ 2x10 cells/mL Specifications Optimal range 4x10 ~ 1x10 cells/mL Analysis time* Please refer to following table. Voltage DC12V Current Objective lens 4W Green LED Camera CMOS camera Filter Excitation filter, Dichroic filter, Emission filter Weight 7 kg 277 ×... -
Page 34: Product List
Product list Product ADAM-MC Fluorescence cell • Main device list • User manual Plus analyzer • 20 mLⅹ1 bottle (400 Tests) APAD-400 Cell viability reagent • Acridine orange (AO) & 4’,6-diamidino- 2-phenylindole (DAPI) stain • 4ch. Slide 100ea AP4S-100 AccuPlus Slide 4ch. ADAM MC Plus... -
Page 35: Safety Precautions
Safety precautions Review and follow the safety instructions below: • If water or other material enters the instrument, the adaptor, or power inlet, disconnect the power cord and contact a service person. For operating environment, refer to Product Specifications. • Do not touch the main plug or power cord with wet hands. •... -
Page 36: Mesures De Sécurité
Mesures de sécurité Examiner et suivre les instructions en matière de sécurité ci-dessous: • Si de l'eau ou d'autres matières entrent dans l'instrument, l'adaptateur, ou l‘entrée de la prise, débrancher le cordon d'alimentation et contacter un technicien de service. Pour l'environnement d'exploitation, se reporter aux Spécifications du Produit. -
Page 37: Safety Symbols
Safety symbols The following symbols are found on the instrument and this Safety document. symbols Always use the equipment in the safest possible manner. Symbol Meaning Caution & Warning Protective earth (Ground) Power On/Off The moving parts symbol indicates areas of the medical device in which moving parts can cause injuries. -
Page 38: Warnings
Warnings 1. After using device, please turn off main power. Warning If not, it may cause malfunction or may reduce product life. 2. When turning off the device, be sure to lock the device with Lock button. If not, it may cause mechanical problem or error message when device is booting. -
Page 39: Technical Support
Technical support ADAM MC Plus... - Page 40 NESMU-AMCP-001E(V.0.1) NanoEntek, Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si Gyeonggi-do, 18531, Korea Tel: +82-2-6220-7940 Fax: +82-2-6220-7999 NanoEntek America, Inc. 220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA Tel: +1-781-472-2558 Fax: +1-781-790-5649 E-mail sales@nanoentek.com Website www.nanoentek.com...
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