Troubleshooting - NanoEnTek ADAMII User Manual

Bench-top fluorescence cell counter
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Troubleshooting

Installation
ADAMII™
does not power on
Operator program
does not start
Camera preview
Camera preview fail
Operation
Warning message
when there are more than
1,000 cells in 1 frame
Total CD45 count is higher than
bright count (WBC count)
Total CD45 count is lower than
bright count (WBC count)
Frame becomes out of focus
towards the end
Frame is in focus and then out of
focus. The following frame is in fo-
cus again. It happens repeatedly.
Focusing is not adjusted well
with autofocus function
Background of image is
suddenly brighten
ADAMII™ ©2016 NanoEnTek Inc.
• Check on/off switch on back side of main instrument.
• Check power source or contact your distributor
• Check on/off switch on back side of main instrument.
• Check connection between instrument and PC
• Check connection between instrument and PC
• Reboot the instrument or PC
• Request technical support
• Dilute the cell concentration less than 1,000 cells/μL
• Recommend 500 ~ 900 cells in 1 frame
• Delete image that has bubble, fluorescence debris or dust,
and then check the result
• Check the focus of image
• If the focus of image was not set properly, load and
read the sample again
• Change the slide and read again
• If you are still having problem, request technical support
• Request technical support
• Set the focus with 2ch manual focus function
• First, check the sample type
• Second, if you used the bottom of ADAMII™ CD34 Reagent
that almost used, please use the new reagent
• If none of the above 2 steps solve the problem, please
request technical support
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