NanoEnTek ADAM-SCC2 Instruction Manual

Automated somatic cell counter

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ADAM
2
SCC
Automated Somatic Cell Counter
Instruction Manual
Copyright
NanoEnTek, Inc. All rights reserved.

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Summary of Contents for NanoEnTek ADAM-SCC2

  • Page 1 ADAM Automated Somatic Cell Counter Instruction Manual Copyright NanoEnTek, Inc. All rights reserved.
  • Page 3 Fax. +1-781-790-5649 The information in this manual is described as accurately as possible. Firmware and software changes and updates may change without prior consent or notification. Copyright © 2019 by NanoEnTek Inc. All rights reserved. Published in Korea. Documentation: NESMU-ASC2-001E Revision history: V.0.0 AUG 2019...
  • Page 5: Table Of Contents

    Lock ADAM-SCC2 Power off SOMA Chip kit Upon receiving the instrument Maintenance and cleaning Trouble shooting Product Description Warranty Front view of ADAM-SCC2 Technical specifications Rear view of ADAM-SCC2 Product list Getting Started Safety precautions Safety symbols Environmental requirements Warnings...
  • Page 6: General Description

    ADAM-SCC2 counts the number of somatic cells to determine the quality of raw milk with unique image cytometer technology. ADAM-SCC2 can be used as an ideal equipment in the research field, dairy farms, milk manufacturing facilities, and veterinary institutes working with somatic cell analysis.
  • Page 7: Technology

    Microfluidic technology of disposable microchips provides low reagent consumption and combining with the ready-to-use reagent makes daily work easy. ADAM-SCC2 is not only easy to use but offers the same repeatability and accuracy as the conventional expensive device. SOMA chip...
  • Page 8: Somatic Cell Count Kit

    Introduction Somatic Cell Somatic Cell Count Kit of ADAM-SCC2 is composed of Propidium Iodide (PI) Count Kit for counting somatic cells. It can be used without diluting raw milk. Measuring range of cell density is 0.05 ~ 1.15 x 10 cells/mL.
  • Page 9: Adam-Scc2

    Power cord Adapter SOMA Chip Kit Calibration Bead Barcode scanner SOMA Chip kit The contents of the ADAM-SCC2’s Somatic Cell Count Kit are listed below: Item SOMA Chip 2x Kit SOMA Chip 4x Kit (Cat. No: CRS-K01) (Cat. No: CRS-K02)
  • Page 10: Front View Of Adam-Scc2

    Product Description Introduction Front view of The front view showing various parts of the ADAM-SCC2 is shown below: ADAM-SCC2 ① Door ② Power ③ ④ ⑤ ⑥ Control buttons Description ① Door Slide holder is inserted and ejected. ② Power Power on / off.
  • Page 11: Rear View Of Adam-Scc2

    Introduction Product Description Rear view of The rear view showing various parts of the ADAM-SCC2. ADAM-SCC2 ① ② ③ USB Port PC Port Power plug Port Description ① USB Port Port for software update and save the data. ③ Power Plug Connects ADAM-SCC2 power cord to wall outlet.
  • Page 12: Environmental Requirements

    •Do not tilt the device too much in the forward when connecting the power cord. •Do not move the device after connecting power cord. - When you connect the power cord to ADAM-SCC2 even without power on the device, it will go through self diagnostic tests.
  • Page 13: Error Messages During Botting

    [System State] during booting It appears when booting is not working properly. Turn off main power and restart device. If this message still appears after restarting, contact your local distributor or sales@nanoentek.com. Error code Cause 0x00000C00 Failure of X-axis sensor...
  • Page 14: Count Setting

    Getting Started Introduction Count setting Set the conditions in the ‘Setting’ tap before counting. [SOMA Chip] Set the SOMA Chip according to you are using. Somachip Somachip [Cell size] Set the minimum and maximum size of cell. Cell size [Dilution factor] When diluting sample, set the Dilution factor.
  • Page 15: Introduction

    100 μL 100 μL 2. Load the mixed sample. Then, wait 1 minute for the sample settling. - 2 channel: 23μL - 4 channel: 13μL 3. Insert SOMA Chip. Get the result. - Result - ADAM-SCC2, Automated Somatic Cell Counter...
  • Page 16: Sample Preparation

    General Operation Introduction Sample 1. Prepare some raw milk sample, SCC-Solution, tube, pipette, and tips. preparation Raw milk Tube SCC-Solution 2. Thoroughly mix the raw milk sample. 1. Add 100 μL of raw milk sample and 100 μL of SCC-Solution in the tube. Counting Cell (1:1 ratio) Milk sample...
  • Page 17 Be cautious of loading the correct volume of the sample into SOMA Chip. The instrument will not detect low or high sample volumes. Correct volume Low volume CAUTION Avoid bubbles which may negatively affect the result. ADAM-SCC2, Automated Somatic Cell Counter...
  • Page 18 General Operation Introduction Counting cell WARNING [SOMA Chip insert error] Completely insert SOMA Chip face up, in the direction of the arrow on the slide. The instrument will not detect if slides are inserted incorrectly. See pictures below for proper insertion. ( O ) ( X ) Correctly inserted...
  • Page 19: Run Sample

    Start counting process by pressing ‘START’ . It may take about 2 minutes longer for auto focus at the initial test. 9010 7436 While the test is in progress, you can check the cell images of each channel. ADAM-SCC2, Automated Somatic Cell Counter...
  • Page 20: Result Analysis

    Measure Introduction Result Analysis The result will be displayed after being automatically calculated by ADAM- SCC2 software. CLOSE Title Count 1.04 x 10E6 1.15 x 10E5 4.68 x 10E5 2.42 x 10E5...
  • Page 21: Result Analysis -Error Code

    Frames with errors are over 50% of total counting frame. Frame with error is a frame that contains cells whose diameter is larger than 100μm. Error frame [#] When this error shown in result window, please check the image. ADAM-SCC2, Automated Somatic Cell Counter...
  • Page 22: Data List

    Data Introduction Data list ① ② ③ ④ ⑤ ⑥ ⑦ Control buttons Description ① Select all data in Data List. ② SEARCH Display the data of the selected date. ③ View and edit the data. EDIT Multiple data can be edited with the same settings. ④...
  • Page 23: Edit

    Cell size setting Set the min/max size of the cell. ④ Set the dilution factor of sample. Dilution Factor Factor values for the SCC-Solution is already applied. ⑤ View the counted cell number each frame. Frame graph ADAM-SCC2, Automated Somatic Cell Counter...
  • Page 24: Image

    Introduction Data IMAGE ① ② ③ ④ Control buttons Description ① Original Check the original image. ② Counted Check the counted cell image. ③ Frame Select a frame number of the channel. ④ Zoom-in/out Zoom in and out to check the cell image.
  • Page 25: Mail

    If you want to receive files by new e-mail, please enter the Mail Address e-mail address. ③ Send the files to a selected e-mail address. Files can be sent Send to only one e-mail at a time. ADAM-SCC2, Automated Somatic Cell Counter...
  • Page 26: Setting

    Setting Introduction Setting ① ⑤ ② ⑥ ③ ④ ⑦ Control buttons Description ① Set the conditions in the setting tap before counting. Count setting Refer to page 10 for more information. ② Dilution factor Set the dilution concentration of the sample. ③...
  • Page 27: Wifi

    • The ‘AdamUpdate’ folder must exist in the root path of the USB folder. • ADAM-SCC2 can be updated only when the firmware or software file exists in the ‘AdamUpdate’ folder. The ‘ADAM SCC2.exe’ file should be in the ‘AdamUpdate’ folder.
  • Page 28: Lock

    Power off Setting Introduction Lock Press LOCK before turning off the device. If there is no operation for 3 minutes, the lock function will be activated automatically. When the device is locked, the screen will be changed as shown below. Power off If you press the power button 2~3 seconds, then ‘Slide to shut down your PC’...
  • Page 29: Maintenance And Cleaning

    1. ADAM-SCC2 does not need regular maintenance. 2. ADAM-SCC2 has no replacement of consumable materials. and cleaning 3. Please clean the exposed surface of ADAM-SCC2 frequently or before testing, using a soft cloth and isopropyl alcohol or deionized water. CAUTION Dispose of wipes in an appropriately labeled solvent contaminated waste container.
  • Page 30: Trouble Shooting

    Trouble shooting Introduction Trouble Problem Description Solution shooting ADAM-SCC2 does • • No power from outlet. Check power source. not power up • • Bad power cord. Replace. Inaccurate result • • Cell number may be out of Adjust the number of cells range.
  • Page 31: Warranty

    Warranty Introduction Warranty If any defects occur in the ADAM-SCC2 during one(1) year warranty period, NanoEnTek will repair or replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded: 1. Defects caused by improper operation.
  • Page 32: Technical Specifications

    Technical Specifications Introduction Technical ADAM-SCC2 Specifications Measuring range 0.05 ~ 1.15 x 10 cells/mL : For initial test, Analysis time < 13 ~ 25 sec/test max 2 min/test Voltage DC12V Current Objective lens 4W Green LED Camera CMOS camera Filter...
  • Page 33: Product List

    25 mL SCC-Solution 100 pcs SOMA Chip 4X CRS-K02 SOMA Chip 4X Kit 25 ml SCC-Solution ADB-500 ADAM Calibration Bead 5 mL Calibration Bead *SOMA Chip 2x Kit: please consult your distributor or manufacture for availability. ADAM-SCC2, Automated Somatic Cell Counter...
  • Page 34: Safety Precautions

    Safety Precautions Introduction Safety Review and follow the safety instructions below : Precautions Always ensure that the power supply input voltage matches the voltage • available at your location. To avoid the danger of electric shock, install the instrument per the •...
  • Page 35: Safety Symbols

    For more detailed information about disposal of your old appliance, please contact your city office, waste disposal service or visit our web-site, www.nanoentek.com. This product conforms to UL 61010-1, CAN/CSA C22.2 No.61010-1 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part l: Gen- eral Requirements.”...
  • Page 36: Warnings

    Warnings Introduction Warnings 1. After using device, please turn off main power. If not, it may cause malfunction or may reduce product life. 2. When turn off the device, be sure lock the device with Lock button. If not, it may cause mechanical problem or error message when device is booting.
  • Page 37: Technical Support

    Technical Support Introduction Technical Visit the our Website at www.nanoentek.com for : Support Technical resources, including manuals, FAQs, etc. • Technical support contact information. • Additional product information and special offers. • For more information or technical assistance, please call or email.
  • Page 40 SCC 2 ADAM ADAM NESMU-ASC2-001E (V.1.0) NanoEnTek, Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel :+82-2-6220-7940 Fax:+82-2-6220-7999 NanoEnTek America, Inc. 240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA Tel: +1-781-472-2558 Fax: +1-781-790-5649 Email sales@nanoentek.com Website www.nanoentek.com...

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