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NanoEnTek ADAM-rWBC Instruction Manual

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DigitalBio is brand name of NanoEnTek.

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Summary of Contents for NanoEnTek ADAM-rWBC

  • Page 1 Instruction Manual DigitalBio is brand name of NanoEnTek.
  • Page 2 The information in this manual is described as accurately as possible. Firmware and software changes and updates may change without prior consent or notification. Copyright © 2013 by NanoEnTek Inc. All rights reserved. Published in Korea. Protected by patents US 7,842,157; US 7,411,680; CN 2004 8 0020890.7;...

  • Page 3: Table Of Contents

    Table of Contents Introduction General description Technology - Mechanical Technology - Viability measurement Product description Packing list Front view of the ADAM-rWBC Rear view of the ADAM-rWBC System installation 10-12 Environmental requirements Power on and initial display Icon functions General operation...

  • Page 4: Introduction

    Introduction ADAM-rWBC is an analyzing device that counts the number of General residual leukocytes in a blood component for transfusion. description Targeting the leukocyte, the ADAM-rWBC uses fluorescent dye to per- form cell dyeing and automated cell counting. Automated cell counting eliminates user bias or subjective interpretation that can be found when counting residual leukocytes using other methods.

  • Page 5: Technology - Mechanical

    LED optics and CCD detec- tion to make provide accurate and reliable leukocyte detection. To use the ADAM-rWBC, a blood sample is mixed with a Propidium Iodide (PI) stain and directly pipetted onto a disposable plastic slide.

  • Page 6: Technology - Viability Measurement

    Introduction ADAM-rWBC is based on the staining of mammalian cell DNA using the Technology fluorescent dye, Propidium Iodide (PI), a nucleic acid dye. When used - Viability with RNase, PI stains only cellular DNA. In the case of blood samples,...

  • Page 7: Product Description

    Product description The ADAM-rWBC is shipped with the components listed in the table follow. Packing list Once you receive your instrument, please check that all items listed follow were shipped. If any items are missing or damaged, contact your local distributor or ivdst@nanoentek.com.

  • Page 8: Front View Of The Adam-Rwbc

    Eject rWBC Door Control buttons Eject • Ejects the slide from the ADAM-rWBC. • Functions as load and unload. Start • Performs all procedures of automatic counting. Lock • Protects the alignment of stage from external shock when the ADAM-rWBC is being moved.

  • Page 9: Rear View Of The Adam-Rwbc

    ADAM-rWBC ON/OFF power Power plug Connects the ADAM-rWBC power cord to wall outlet USB port Port for connect to ADAM-rWBC PC software RS232C port Port for only QC and service Keypad port Port for only QC and service ADAM - rWBC ©2013 NanoEnTek Inc.

  • Page 10: System Installation

    (On the rear side of the main device.) • When you turn on the ADAM-rWBC, it will go through self diagnostic tests. If you get an error message, please contact your local distributor or ivdst@nanoentek.com. If boot up is successful and no errors are...
  • Page 11 When you turn off the device, please remove the r-Slide from the ADAM-rWBC. This is the cause of malfunction. If this message continues to display when no r-Slide is inserted, contact your local distributor or ivdst@nanoentek.com. ADAM - rWBC ©2013 NanoEnTek Inc.

  • Page 12: Icon Functions

    [Device unlocked] It appears when turning off the device without lock- ing. Press the lock button before turn off the device. If this message still appears after re-start, contact your local distributor or ivdst@nanoentek.com. Icon functions Display a status of the functions such as Eject, Start, Lock or Insert.

  • Page 13: General Operation

    Refer to the ADAM-rWBC Kit Package Insert for specific instructions. • The ADAM-rWBC must be calibrated after each start up and at least once each day of use. 1. Let beads equilibrate to room temperature before use (~10 minutes).

  • Page 14: Sample Preparation And Testing

    General operation 1. Leave the r-Kit at room temperature for 10 minutes before use. Sample 2. Carefully dispense 100 uL of well-mixed RBC or platelet sample into preparation a clean test tube. and testing 3. Add 400 µL of reagent into the tube. 4.

  • Page 15: Operating The Adam-Rwbc

    Load 100μL of sample into the r-Slide and ensure correct filling. The instrument will not detect in low or high sample volumes. CAUTIO N: • Avoid bubbles as they may negatively affect results. Low volume Correct volume ADAM - rWBC ©2013 NanoEnTek Inc.
  • Page 16 General operation • r-Slide insert error Operating the Completely insert r-Slides face up, in the direction of the ar- ADAM-rWBC row on the slide. The instrument will not detect if slides are inserted incorrectly. See pictures below for proper insertion.

  • Page 17: Start Input

    Starts the counting process by the Start button input. Once the focusing process finished after calibration step, ADAM-rWBC will skip the focusing process for the repeated experiments. Captures the images from the valid r-Slide measuring area and ana- lyzes the images in memory and performs the counting process.

  • Page 18: Maintenance And Cleaning

    ADAM-rWBC does not need regularly scheduled maintenance be- Maintenance cause ADAM-rWBC has no consumable materials to replace. and cleaning Frequent or before every testing, please clean the exposed outer surface of ADAM-rWBC using a soft cloth and isopropyl alcohol or deionized water.

  • Page 19: Trouble Shooting

    Refer to the 11 page message [ System init. ] Refer to the 11 page [ Device unlocked ] Refer to the 12 page Result error [ Bad sample! ] Refer to the 17 page message ADAM - rWBC ©2013 NanoEnTek Inc.

  • Page 20: Warranty

    Warranty If any defects occur in the ADAM-rWBC during one(1) year warranty period, NanoEnTek will re- pair or replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded: Defects caused by improper operation. Repair or modification done by anyone other than NanoEnTek or an authorized agent.

  • Page 21: Technical Specifications

    0 °C ≤ Temperature ≤ 40 °C 10 % ≤ Humidity ≤ 90 % Altitude ≤ 2,000 m Transportation & storage environment condition -30 °C ≤ Temperature ≤ 60 °C 10 % ≤ Humidity ≤ 90 % ADAM - rWBC ©2013 NanoEnTek Inc.

  • Page 22: Product List

    ADAM-rWBC Kit(50 tests) r-Solution 25mL(RDR-50) Standard bead solution 7mL(ADST-001) Accesories Barcode scanner * Each component and power cord are available for purchase separately. Please contact your local distributor or ivdst@nanoentek.com. * This Instruction Manual can be downloaded from the NanoEnTek website, www.nanoentek.com.

  • Page 23: Safety Precautions

    · Use this equipment only as specified in this manual and as specified in any documentation associated with its components. Use of the equipment in an unspecified manner may result in damage to the device or injury to the user. ADAM - rWBC ©2013 NanoEnTek Inc.
  • Page 24 Consignes de securite Examinez et suivez les consignes de securite ci-dessous : · N’installez pas l’instrument dans un endroit humide comme une serre ou un incubateur pour eviter un risque de choc electrique. Si de l’eau ou tout autre materiau penetre dans l’instrument, l’adaptateur, ou l’entrees d’alimentation, debranchez le cordon d’alimentation et contactez un tech- nicien de service.

  • Page 25: Safety Symbols

    “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part l: General Requirements.” Instruments bearing the TUV symbol are certified by TUV Product Services to be in conformance with the applicable safety standard for the US and Canada. ADAM - rWBC ©2013 NanoEnTek Inc.

  • Page 26: Warnings

    Warnings 1) After using device, please turn off main power. If not, it can be cause of malfunction or reduce lifetime of device. 2) When turn off the device, be sure lock the device with Lock button. If not, it can be cause of mechanical problem or error message when device is booting. Item Warning Battery inside device...

  • Page 27: Technical Support

    Technical support Visit the our Website at www.nanoentek.com for : Technical resources, including manuals, FAQs, etc. · Technical support contact information · Additional product information and special offers. · For more information or technical assistance, please call or email. NanoEnTek, Inc.
  • Page 28 ADAM-rWBC NESMU-ARC-001E (V.4.0) DigitalBio is brand name of NanoEnTek. NanoEnTek, Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel. +82-2-6220-7942 Fax. +82-2-6220-7999 NanoEnTek America, Inc. 240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA Tel. +1-781-472-2558 Fax. +1-781-790-5649 EC Representative MT Promedt Consulting GmbH Altenhofstrasse 80, 66386 St.

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