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Related Manuals for NanoEnTek ADAM CellT Plus
Summary of Contents for NanoEnTek ADAM CellT Plus
- Page 2 The information in this manual is described as accurately as possible. Firmware and software changes and updates may change without prior consent or notification. Copyright © 2023 by NanoEntek Inc. All rights reserved. Published in Korea. Documentation: NESMU-ACTP-001E Revision history: V.0.0 DEC 2023...
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Page 3: Table Of Contents
Table of Contents Introduction ........................... 5 General Description......................5 Technology ........................6 Basic ..........................7 principle of counting ......................7 Product contents ........................8 ADAM CellT Plus ......................8 AccuPlus Slide & Reagent ....................8 Upon receiving the instrument ..................8 Product Description ...................... - Page 4 MAIL..........................29 Approval..........................30 Requestable ........................30 Requesting ........................31 Approvable ........................32 Approved ........................33 Setting ........................... 34 Setting ..........................35 Wifi ..........................35 Remote support ......................35 Update .......................... 36 Auto logout ........................36 Backup .......................... 36 Recovery ........................37 User .............................
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Page 5: Introduction
Introduction The ADAM CellT Plus is a new standard of highly accurate General automated cell counter equipped with bright field and two Description fluorescence channels (AO/DAPI). ADAM CellT Plus... -
Page 6: Technology
Also, Trypan Blue exclusion method has been known to overestimate cellular viability. In addition to these limitations, manual counting can be user-dependent. To address these problems, NanoEntek has developed ADAM CellT Plus. ADAM CellT Plus is an image-based fluorescence cell counter. It takes bright field image and two color fluorescence images. -
Page 7: Basic
Introduction CellT Plus uses Acridine Orange (AO) and 4’,6-diamidino- ADAM Basic 2-phenylindole (DAPI) to count total number of cells and number of principle of dead cells, respectively. AO is a cell-membrane permeable dye that stains nucleus of every cells regardless of the cell’s condition. counting Therefore, it is used to count total number of cells. -
Page 8: Product Contents
Product contents ADAM CellT Plus is shipped in a carton box containing followings; ADAM CellT Plus Item Quantity Main device User manual USB hub Wifi dongle Power cord Adapter External Hard disk Test Bead QC slide (optional) PC (only PC Mode) Printer (optional) AccuPlus Slide and reagent has following components: AccuPlus... -
Page 9: Product Description
Product Description The front view showing various parts of the ADAM CellT Plus is Front view shown below: ADAM CellT Plus Control buttons Description ① Door Slide holder is inserted and ejected. ② Power Power on / off. ③ LCD Display processes and results. -
Page 10: Rear View Of Adam Tm Cellt Plus
Product Description The rear view showing various parts of the ADAM CellT Plus.: Rear view ADAM CellT Plus Port Description ① USB Port Port for software update and save the data. ② PC port Connects with PC (Only PC Mode). Connects ADAM CellT Plus power cord to wall ③... -
Page 11: Getting Started
“ADAM CellT icon” to execute the software) Initial Display If you get an error message, please contact your local distributor or sales@nanoentek.com. If booting is successful and no errors are detected, the home screens will be displayed as below. -
Page 12: Error Messages During Booting
[System State] during booting It appears when booting is not working properly. Turn off main power and restart device. If this message still appears after restarting, contact your local distributor or sales@nanoentek.com. Error code Cause Failure of X-axis sensor 0x00000C00 0x00007000... -
Page 13: Count Setting
Getting started Count setting [Cell size] Set the minimum and maximum size of cell. Cell size [Dilution factor] When diluting sample, set the Dilution factor. CAUTION Factor values for the Cell viability reagent is already applied. Dilution factor ADAM CellT Plus... -
Page 14: General Operation
General Operation Instruction is provided in this section for preparing the sample with Instruction Cell viability reagent for use with disposable AccuPlus Slide for automated cell count using the ADAM CellT Plus. Please check the procedure of sample preparation and testing below. -
Page 15: Sample Preparation
General Operation Sample 1. Cultivate the required number of cells. preparation NOTE Concentration out of this range will result in errors. Refer to page 20 for more information about errors. 2. Thoroughly mix the cell pellet by vortexing or pipetting. 3. - Page 16 General Operation Cell counting WARNING [Sample loading error] Be cautious of loading the correct volume of the sample into AccuPlus Slide. The instrument will not detect low or high sample volumes. Correct volume Low volume CAUTION Avoid bubbles which may negatively affect the result. ADAM CellT Plus...
- Page 17 General Operation WARNING [AccuPlus Slide insert error] Completely insert AccuPlus Slide face up, in the direction of the arrow on the slide. The instrument will not detect if slides are inserted incorrectly. See pictures below for proper insertion. WARNING • Please insert or remove the AccuPlus Slide when the slide holder is fully ejected.
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Page 18: Measure
Measure Run sample Start counting process by pressing ‘START’. It may take about 2 minutes longer for auto focus at the initial test. While the test is in progress, you can check the cell images of each channel. ADAM CellT Plus... -
Page 19: Result Analysis
Measure The result will be displayed after being automatically calculated by Result ADAM CellT Plus software. analysis Number of Number of Title Viability Total cell Non-Viable cell Viability 01 1.10E6 5.50E5 Viability 02 2.20E6 5.50E5 • The viability will be automatically calculated by the ADAM CellT Plus software after each measurement of the total cells and the non-viable cells. -
Page 20: Result Analysis - Error Code
Measure Result Analysis - Error code Error code Cause Frames with errors are over 50% of total counting frame. Cells are more than 2 × 10 7 cells/mL. Cells are more than 1 × 10 7 cells/mL. Cells are less than 4 × 10 5 cells/mL. Cells are less than 5 ×... -
Page 21: Qc Mode
The QC Mode uses QC Slide (optional) to check equipment QC QC Mode status by date at a glance. [Activation of QC mode] To activate ‘QC’ mode, an activation code must be entered. 1. Select ‘QC’ tab from top menu. 2. -
Page 22: Qc Slide Edit
QC Slide Edit The QC Mode uses QC Slide (optional) to check equipment QC status by date at a glance. Control buttons Description Selects all QC Slide lot to delete from the QC Slide ① All lot List ② QC Slide List Provides registered QC Slide lot information list ③... -
Page 23: Qc Slide Result Edit
QC Slide Result Edit Control buttons Description ① All Selects all QC result to delete from the QC result List ② QC Slide List QC result information list Selects all QC result to display graph from the QC ③ Visible result List. -
Page 24: Qc Slide Result
• Acceptance slide peak size results can be found in the ADAM CellT Plus Test report. WARNING Contact sales@nanoentek.com or your local distributor if the QC result does not come within the acceptance criteria. ADAM CellT Plus... -
Page 25: Data
Data Data list Control buttons Description ① All Select all data in Data List. ② SEARCH Display the data of the selected date. View and edit the data. ③ EDIT Multiple data can be edited with the same settings. ④ IMAGE Check the cell images of each channel. -
Page 26: Edit
Data EDIT Control buttons Description ① Sample Edit the sample name. ② Cell size graph Allows to view the cell size graph for each channel. Allows to view the number of cells in each cell size. ③ Cell size table T=Total cell (AO), D=Dead cell (DAPI), R=Real cell size(Bright) ④... -
Page 27: Image
Data IMAGE Control buttons Description ① Channel Select a channel. ② Original Check the original image. ③ Counted Check the counted cell image. ④ Frame Select a frame number of the channel. ⑤ Zoom-in/out Zoom in and out to check the cell image. ADAM CellT Plus... -
Page 28: Save
Data SAVE Control buttons Description Selects a save path from the list to send the selected ① Select Path data. ② Data Type Selects which data type to save. Exports the files to a selected save path ③ Apply Files can be sent to only one save path at a time. ADAM CellT Plus... -
Page 29: Mail
Data MAIL Control buttons Description Selects e-mail address from the list to send data ① History The e-mail address where data has been sent will be saved. To send files to new e-mail, enter the applicable e mail ② Mail Address address. -
Page 30: Approval
Approval Requestable Control buttons Description Settings tab related to data approval such as ① Approval Status Requestable, Requesting, Approvable, and Approved. List of data that can check status information related ② Data List to approval. ③ All Select all data in Data List ④... -
Page 31: Requesting
Approval Requesting Control buttons Description Settings tab related to data approval such as ① Approval Status Requestable, Requesting, Approvable, and Approved. List of data that can check status information ② Data List related to approval. ③ All Select all data in Data List ④... -
Page 32: Approvable
Approval Approvable Control buttons Description Settings tab related to data approval such as ① Approval Status Requestable, Requesting, Approvable, and Approved. List of data that can check status information ② Data List related to approval. ③ All Select all data in Data List ④... -
Page 33: Approved
Approval Approved Control buttons Description Settings tab related to data approval such as ① Approval Status Requestable, Requesting, Approvable, and Approved. List of data that can check status information ② Data List related to approval. ③ All Select all data in Data List ④... -
Page 34: Setting
Approval Setting Control buttons Description Set the conditions in the setting tap before ① Count setting counting. Refer to page 14 for more information. Sets the wifi to use the e-mail or remote support ② Wifi function. ③ Remote support Connects to remote support software. -
Page 35: Setting
In PC Mode, please use internet (Windows). Remote support 1. Connect to wifi. 2. Click ‘Remote support’ button. 3. Share your ID and password to NanoEntek. NOTE The remote support feature is to be used for maintenance only by request of NanoEntek. WARNING If you do not see your Remote Support ID and Password, click the ‘... -
Page 36: Update
Setting 1. Prepare the USB with update file. Update 2. Insert the USB. 3. Click the UPDATE button. CAUTION • The ‘AdamUpdate’ folder must exist in the root path of the USB folder. • ADAM CellT Plus can be updated only when the firmware or software file exists in the ‘AdamUpdate’... -
Page 37: Recovery
Setting Recovery • Restore counted data to the point of the last automatic backup (Sync). • Manual recovery restores manually backup counted data. NOTE The automatic backup function is turned off after recovery, please re-enable the automatic backup function. CAUTION Please be careful with manual recovery, because counted data that is not manually backed up will be lost. -
Page 38: User
User User Control buttons Description ① Logout To logout ② User manage To register user ③ Log manage Tracks user access records ④ Document manage Tracks management document records ⑤ Deleted list Tracks deleted data records ADAM CellT Plus provides a comprehensive solution to comply with the requirements of the 21 CFR Part 11 rule. -
Page 39: User Manage
User User manage Control buttons Description ① User Name To view the registered user list. ② Permission To view the user access authority ③ New Registration To register new user ④ Privilege Option for permission setting ⑤ Supervisor Permission To register as a supervisor ⑥... -
Page 40: Privilege
User The Admin can grant or release access to functions when creating Privilege or editing new users (Supervisor, User). Control buttons Description Selects account level and sets default ① User Type&Default permissions ② Tab Tab for permission Settings Detailed functions for each permission ③... -
Page 41: Password Option
User Password Option Control buttons Description ① Change cycles Set password change interval (30, 90, 180 days). Number of incorrect passwords in account lockout ② Account lock (≤ 3, 5, 10, 15). ③ Minimum length Minimum length of password (≤3, 5, 10, 15). Prohibition of using the same password for a ④... -
Page 42: Log Manage
User manage Control buttons Description ① All Select all data in Data List. ② SEARCH Display the data of the selected date. ③ SAVE Save the selected data to USB. ④ DELETE Delete the selected data. ⑤ CLOSE Close the log manage. NOTE The search period is limited to 90 days (Except PC Mode). -
Page 43: Document Manage
User Document manage Control buttons Description ① All Select all data in Data List. ② SEARCH Display the data of the selected date. ③ SAVE Save the selected data to USB. ④ DELETE Delete the selected data. ⑤ CLOSE Close the log manage. NOTE The search period is limited to 90 days (Except PC Mode). -
Page 44: Deleted List
User Deleted list Control buttons Description ① All Select all data in Data List. ② SEARCH Display the data of the selected date. ③ SAVE Save the selected data to USB. ④ DELETE Delete the selected data. ⑤ CLOSE Close the deleted list. NOTE The search period is limited to 90 days (Except PC Mode). -
Page 45: Power Off
Power off Lock Press LOCK before turning off the device. If there is no operation for 1 minute, the lock function will be activated automatically. When the device is locked, the screen will be changed as shown below. Power off If you press the power button 2~3 seconds, then ‘Slide to shut down your PC’... -
Page 46: Maintenance And Cleaning
Maintenance and cleaning 1. ADAM CellT Plus does not need regular maintenance. Maintenance 2. ADAM CellT Plus has no replacement of consumable materials. and cleaning 3. Please clean the exposed surface of ADAM CellT Plus frequently or before testing, using a soft cloth and isopropyl alcohol or deionized water. -
Page 47: Trouble Shooting
Trouble shooting Trouble Problem Description Solution ADAM CellT • No power from outlet • Check power source. shooting Plus does not • Bad power cord. • Replace. power up Inaccurate result • Cell number may be • Adjust the number of cells to recommended out of range. -
Page 48: Warranty
2. Repair or modification done by anyone other than NanoEntek or an authorized agent. 3. Damage caused by substituting alternative parts. 4. Use of fittings or spare parts supplied by anyone other than NanoEntek. 5. Damage caused by accident or misuse. 6. Damage caused by disaster. -
Page 49: Technical Specifications
Technical specifications ADAM CellT Plus Technical Measuring range 5x10 ~ 2x10 cells/mL Specifications Optimal range 4x10 ~ 1x10 cells/mL Analysis time* Please refer to following table. Voltage DC12V Current Objective lens Green, Blue, UV Camera CMOS camera Filter Excitation filter, Dichroic filter, Emission filter Weight 7 kg 277 ×... -
Page 50: Product List
Product list Product list ADAM Fluorescence cell • Main device • User manual CellT Plus analyzer • 20 mLⅹ1 bottle (400 Tests) Cell viability • Acridine orange (AO) & 4’,6- APAD-400 reagent diamidino-2-phenylindole (DAPI) stain AccuPlus Slide • 4ch. Slide 100ea AP4S-100 4ch. -
Page 51: Safety Precautions
Safety precautions Review and follow the safety instructions below: • If water or other material enters the instrument, the adaptor, or power inlet, disconnect the power cord and contact a service person. For operating environment, refer to Product Specifications. • Do not touch the main plug or power cord with wet hands. •... -
Page 52: Mesures De Sécurité
Mesures de sécurité Examiner et suivre les instructions en matière de sécurité ci-dessous: • Si de l'eau ou d'autres matières entrent dans l'instrument, l'adaptateur, ou l‘entrée de la prise, débrancher le cordon d'alimentation et contacter un technicien de service. Pour l'environnement d'exploitation, se reporter aux Spécifications du Produit. -
Page 53: Safety Symbols
Safety symbols The following symbols are found on the instrument and this document. Safety Always use the equipment in the safest possible manner. symbols Symbol Meaning Caution & Warning Protective earth (Ground) Power On/Off The moving parts symbol indicates areas of the medical device in which moving parts can cause injuries. -
Page 54: Warnings
Warnings 1. After using device, please turn off main power. Warning If not, it may cause malfunction or may reduce product life. 2. When turning off the device, be sure to lock the device with Lock button. If not, it may cause mechanical problem or error message when device is booting. -
Page 55: Technical Support
Technical support ADAM CellT Plus... - Page 56 NESMU-ACTP-001E (V.0.0) NanoEntek, Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si Gyeonggi-do, 18531, Korea Tel: +82-2-6220-7940 Fax: +82-2-6220-7999 NanoEntek America, Inc. 220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA Tel: +1-781-472-2558 Fax: +1-781-790-5649 E-mail sales@nanoentek.com Website www.nanoentek.com...
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