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UGO BASILE 37370 Instruction Manual

Plantar test hargreaves apparatus
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instruction manual
Plantar Test (
)
Hargreaves Apparatus
Cat. No. 37370
UGO BASILE S.R.L.
sales@ugobasile.com / service@ugobasile.com www.
.com
ugobasile

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Summary of Contents for UGO BASILE 37370

  • Page 1 Plantar Test ( Hargreaves Apparatus Cat. No. 37370 UGO BASILE S.R.L. sales@ugobasile.com / service@ugobasile.com www. .com ugobasile...
  • Page 2 THE INSTRUMENT IN SAFE CONDITIONS. SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED PERSON- NEL, AUTHORIZED BY UGO BASILE ORGANIZATION. ANY ADJUSTMENT, MAINTENANCE AND REPAIR OF THE OPENED INSTRUMENT UN- DER VOLTAGE SHOULD BE AVOIDED AS MUCH AS POSSIBLE AND, WHEN INEVITA- BLE, SHOULD BE CARRIED OUT BY A SKILLED PERSON WHO IS AWARE OF THE HAZARD INVOLVED.
  • Page 3 (no visual score needed!) Optional 37300 Radiometer for calibration I.R. intensity adjustable in the interval 01-99 (in one digit steps) Data portability via the included memory key Software included NEW: orofacial stimulation by optional holders Ugo Basile: more than 10,000 citations...
  • Page 4 CHECK-LIST Cat. No. 37370 Plantar Test (Hargreaves Apparatus) CLIENTE / CUSTOMER______________________________________________ Ordine No. / Order No. ____________________ Data / Date____/_____/______ UB code  CAT.No. Q.ty DESCRIPTION DESCRIZIONE 37370-001 CONTROL UNIT UNITÀ DI CONTROLLO 37370-002 MOVABLE I.R. SOURCE UNITÀ DI STIMOLAZIONE I.R.

  • Page 5: Table Of Contents

    Page i 37370 Instruction Manual (Rev. 0) C O N T E N T S GENERAL ......................1     ..................1 RINCIPLE OF PERATION     INSTRUMENT DESCRIPTION ................2     I.R. S ........... 2 OVABLE OURCE TIMULATOR  ...
  • Page 6 Page ii 37370 Instruction Manual (Rev. 0) ....... 12 OW TO ET A USTOMIZED XPERIMENTAL ONFIGURATION     5.6.1  13  EQUENCE UMBER 5.6.2  14  XPERIMENT UMBER 5.6.3  14  NIMAL ENDER 5.6.4  14  ..................14 ONFIGURATION     5.7.1  15  ROM THE...
  • Page 7 12.1 ..................... 28 PARE PARTS     12.2 ......................28 PTIONAL     12.3 ..................29 HERMAL RINTER     BIBLIOGRAPHY ....................29     13.1 ....................29 ETHOD APER     13.2 ..........29 APERS ENTIONING ASILE LANTAR    ...

  • Page 8: General

    Page 1 37370 Instruction Manual (Rev. 0) Plantar Test (Hargreaves Apparatus) Cat. 3 7 3 7 0 GENERAL The Hargreaves’ Method (see paragraph 12-BIBLIOGRAPHY) enables the researcher to discern a peripherally mediated response to thermal stimulation caused by drugs in the unrestrained rat.

  • Page 9: Instrument Description

    The I.R. Vessel glides on the large base platform 37000-003. The Glass Pane 37370- is inserted into a consistent frame, whose external dimensions are 86(w)x35(d)cm, held in position by its own weight on the top of the four supporting columns 37370-327, fitted to the platform.

  • Page 10: Animal Enclosure

    Page 3 37370 Instruction Manual (Rev. 0) 2.2.1 Animal Enclosure 37000-006 A modular enclosure original design, combines 3 animal cages. Removable partitions Removable partitions enable the us- er to divide each of the 3 cages into 2 or 4 spaces, thus obtaining up to 12 spaces.

  • Page 11: Graphic Display

    Page 4 37370 Instruction Manual (Rev. 0) The graphic display presents all available commands: the operator sets the experiment configuration via the command keyboard located on the right of the display. 2.3.2 Graphic Display When the instrument is switched on, our logo with the two mice is displayed.

  • Page 12: Data Acquisition & Data Print-Out

    Windows® based Data Acquisition Software Package, which ena- bles the research worker to route to the PC the data originated by all Ugo Basile instru- ments and store them into individual files, ready to be easily managed by most statistical analysis packages available on the market.

  • Page 13: Panel-Mount Printer 37000-145

    Page 6 37370 Instruction Manual (Rev. 0) Panel-Mount Printer 37000-145 The 37370 Plantar Test can be provided with a panel-mount printer, Cat. No. 37000-145, to print the experimental data on its 58 mm wide paper, see Figure 2. The printer can be or-...

  • Page 14: Notes On The Instruction Manual

    Page 7 37370 Instruction Manual (Rev. 0) Protect the environment! Dispose of packaging properly, according to existing and ap- plicable waste management rules and regulations. Notes on the Instruction Manual The Plantar Test Instruction Manual included in the package (on the USB pen-drive) is necessary for the correct installation and operation of the instrument.

  • Page 15: Environment

    Page 8 37370 Instruction Manual (Rev. 0) Once the Plantar Test is assembled, do not attempt to lift or move the combined unit. In particular, do not incline the base, as the framed glass may accidentally come off the supporting columns, with the consequences you may imagine! Environment Avoid direct illumination of the enclosure compartments, e.g., from a...

  • Page 16: Mains Cord

    Page 9 37370 Instruction Manual (Rev. 0) 4.5.3 Mains Cord It is a standard cable, Cat. # E-WP008, see paragraph 12-ORDERING INFORMATION. Make sure your power outtake is provided with a reliable ground connection. Connections Connect the mains cord to a power outtake, provided with a reliable earth connection, protected by a differential earth-leakage switch (CGFI) that breaks at the threshold of 0.03A, within a max.

  • Page 17: Installation Of Cub Software

    Do not spray any liquid on the connectors; h. Keep inflammables far from the heating source. UGO BASILE DOES NOT ACCEPT ANY RESPONSIBILITY FOR PROBLEMS OR HARM CAUSED TO THINGS OR PERSONS, ARISING FROM:  incorrect electrical supply;...

  • Page 18: Operation

    OPERATION Switching On Switch on the 37370; the software takes some seconds to perform a complete checking of the electronic circuit. During the checking, the display shows the mice logo. By de- pressing ESC, the display shows the software version which is actually installed, e.g., “V1.0”, see paragraph 2.3.2.

  • Page 19: Carrying Out The Test On The Animal

    Be patient and dry the glass pane before the first test and at least between each test. Durham Holders for Orofacial Stimulation The Durham Animal Holders are the newest accessory for use with the Plantar Test and Dynamic Plantar Aesthesiometer, manufactured by Ugo Basile. These animal holders extend the scope of the infrared (IR) thermal...

  • Page 20: Sequence Number

    Page 13 37370 Instruction Manual (Rev. 0)  experiment number (see 5.6.2)  animal gender (see 5.6.3) From the main menu, depress the F2 (FNC) key, followed by the F1 (OUT) key. Depress again the key F2 (FTR); the display shows the first setting, the sequence number.

  • Page 21: Experiment Number

    Page 14 37370 Instruction Manual (Rev. 0) 5.6.2 Experiment Number As soon the F4 (OK) or F3 (NUL) keys are depressed, followed by the F4 key again, the second option is highlighted. The experiment number, from 1 to 9, can...

  • Page 22: From The Memory Key To The Instrument

    Page 15 37370 Instruction Manual (Rev. 0) The involved parameters are:  I.R. Intensity  Cut-Off Time  Experiment Number  Gender  Sequence Number  MiniPrinter 5.7.1 From the Memory Key to the Instrument With the memory key inserted in its slot on the front panel, depress F1 (OPR), F2 (SET) and F3 (CFG).

  • Page 23: Downloading

    PC (even when the PC is not connected). USB and serial communication between the Plantar Test and the PC is managed by the Ugo Basile CUB (or Win-DAS) software, see paragraph 3 and software manual. How to set the Data output to MiniPrinter The experimental data are saved in the instrument internal memory.

  • Page 24: Datum Format

    Page 17 37370 Instruction Manual (Rev. 0) In case of operation in conjunction with the Mini-Printer the operator has to select the data output path menu. From the main menu, depress F2 (FNC) key, followed by the F1 (OUT) key. The display shows:...

  • Page 25: Quick Setting Of The 37370 Plantar Test

    = Animal gender = Sequence number = Test OK Quick setting of the 37370 Plantar Test This Instruction Manual extensively explains how to take advantage of all the features of- fered by this microprocessor controlled unit. The following table summarizes them; how- ever, we recommend you to browse the complete manual to go deep into details.

  • Page 26: Memory Management

    Page 19 37370 Instruction Manual (Rev. 0) MEMORY MANAGEMENT Each datum point, no matter whether print- ed or sent to the PC, is saved in the memory (which can store about 300 measures, including the datum proper and the date/time indication).

  • Page 27: Memory Reset

    Page 20 37370 Instruction Manual (Rev. 0) To interrupt the print-out response of the stored data, depress the F4 (ESC) key for one second at least. Before starting a new experimental session, we suggest checking the memory which may contain some data of the previous trial.

  • Page 28: Setting Time & Date

    Page 21 37370 Instruction Manual (Rev. 0) Setting Time & Date To set time and date, from the Main menu depress the F2 (FNC) key, followed again by the F2 (CLK) key. Use the F3 horizontal arrow key to highlight the parameter to be modified.

  • Page 29: Radiometer

    Page 22 37370 Instruction Manual (Rev. 0) Radiometer The Radiometer enables us to deliver, and the user to maintain, the I.R. source of each Plantar test with identical power flux output (mW per square cm). There is no way to calibrate the Plantar Test (as any other I.R. emitting device) without a Radiometer;...

  • Page 30: Calibration Without I.r. Radiometer

    The default calibration value is thus recalled. MAINTENANCE While any service of the instrument ought to be carried out by Ugo Basile personnel or by qualified personnel authorized by UGO BASILE organization, this section of the instruc- tion manuals describes normal maintenance procedures which can be carried out at your...

  • Page 31: Electrical

    Page 24 37370 Instruction Manual (Rev. 0) UNPLUG THE MAINS CORD BEFORE CARRYING OUT ANY MAINTENANCE JOB! 10.1 Electrical To inspect and/or replace the fuses, disconnect the mains cable first! Insert a miniature screwdriver in the slot indentation, see paragraph 4.5.1, and snap out the slide which houses the fuses.

  • Page 32: Replacing The Bulb

    Page 25 37370 Instruction Manual (Rev. 0) Insert the fresh snaphat paying attention to its orientation; its white dot should match the dot on the PCB. A “click” assures you that a proper insertion took place. Reassemble the bottom panel.

  • Page 33: Cleaning The Glass Pane

    Pay attention when handling the glass-pane! In case of breakage, use heavy duty gloves to handle the glass pieces and chips. Spare glass panes 37370-005 (see paragraph 12) are stocked at our factory, each com- plete with its frame. 10.6...

  • Page 34: Instrument Specifications

    Page 27 37370 Instruction Manual (Rev. 0) service@ugobasile.com logistics@ugobasile.com sales@ugobasile.com Before sending any instrument to our factory for repair, please contact our logistics department to obtain a return authorization number (RMA) and shipping/packing instruc- tions. We may not be held responsible for damages during transport due to poor packing;...

  • Page 35: Ordering Information

    12.2 Optional 37300 Heat-Flux I.R. Radiometer (see also paragraph 9.1) 37370-278 Additional Stimulation Base, complete with glass pane and animal enclosure 37100 Set of 2 Durham Holders for Orofacial Stimulation (see paragraph 5.5) 37000-145 Panel-Mount Printer (see also paragraph 3.2) 57145 MiniPrinter (see also paragraph 3.3 and 12.3)
  • Page 36 Page 29 37370 Instruction Manual (Rev. 0) 12.3 Thermal MiniPrinter The Mini-Printer 57145 is a ther- mal array device, for easy con- nection to the 37140 , and other Ugo Basile devices, to print ex- perimental data on its 58mm wide paper Cat.
  • Page 37 Page 30 37370 Instruction Manual (Rev. 0) V. Carozzi et alia: “Chemotherapy-Induced Peripheral Neurotoxicity in   Immune-Deficient Mice: New Useful Ready-to-Use Animal Models” Exp. Neurology 264: 92-102, 2015 V. Vacca et alia: “Botulinum Toxin A Increases Analgesic Effects of Mor- ...
  • Page 38 Page 31 37370 Instruction Manual (Rev. 0) _______________________________________________________________________ INSTRUCTION MANUAL October 2013 REVISION 0...
  • Page 40 APPENDIX 37370 Instruction Manual (Rev. 0) APPENDIX Foot Withdrawal Response to Noxious Radiant Heat in the Rat In the living animal, the temperature at the target site, to which the infrared (IR) beam is applied, is definitely an elusive datum.
  • Page 41 N o t e s _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _...
  • Page 42 N o t e s _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _...
  • Page 43 N o t e s _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _...
  • Page 44 CE CONFORMITY STATEMENT Manufacturer UGO BASILE srl Address Via G. di Vittorio, 2 – 21036 Gemonio, VA, ITALY Phone n. +39 0332 744574 Fax n. +39 0332 745488 We hereby declare that Instrument. PLANTAR TEST Catalog number 37370 It is manufactured in compliance with the following European Union Directives and relevant harmonized standards ...

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