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1.0 General Description
The NuAire US Autoflow Automatic CO
vitro environment for optimum tissue cell culture growth. The chamber also provides an environment for the storage
and preservation of gametes and animal tissue cell cultures at on near body temperature. Six parameters contribute to
optimum growth conditions. These are:
1.
Humidity
2.
Precise temperature control
3.
Precise CO
2
4.
Sterility
5.
Precise O
control
2
6.
Reliability
Like all NuAire equipment, the US Autoflow has been designed to provide the highest quality standards of performance
with matching computer technology, precise temperature control and CO
art technology with years of design, quality and manufacturing experience.
In order to accomplish the foregoing objectives, the US Autoflow features the following:
1.1 Extra Large (20 gallon) Water-Jacket - Each Chamber
The outer stainless steel wall is lined with a space-age insulation providing a R5.0 rating, minimizing heat loss.
The large 20 gallon (75.7 liters) water-jacket utilizes water, one of natures best "sinks" of heat. Its high
capacity to hold heat makes it the ideal medium to surround a chamber in order to obtain temperature
uniformity. In fact, the ability of materials to hold heat, called the specific heat, uses water as the
comparative standard. The large water-jacket surrounding the chamber permits the water to circulate within
the jacket, producing a temperature uniformity of ±0.2°C. The larger the mass, the less susceptible the
environment within the chamber is to environment fluctuations outside. It also adds cabinet stability for the
growth of vibration sensitive cells.
1.2 NuAire Incubator Control Electronics
The NuAire Incubator Control Electronics (NICE) is a state-of-the-art microcomputer based control system
specifically designed to service the precise control requirements of the chambers environment, providing
optimum programmable conditions for culture growth. The microcomputer is "user friendly" with status
indicators, LED display of control parameters, Hidden key and three touch control key pads to permit efficient
operator entry of data.
The microcomputer is supported with Read Only Memory (ROM) containing executable software, Random
Access Memory (RAM) for temporary storage and Electronically Erasable Programmable Read Only Memory
(EEPROM) for control set points and parameters. The EEPROM provides for indefinite storage of these values
during periods of power off or power interruption (power fault tolerant).
The microcomputer includes a complete internal diagnostic software package that permits fault isolation
detection down to the failed component.
OM0123
Rev 15
US Autoflow Automatic CO
Water-Jacketed Incubator
Models NU-4750/D/E, NU-4850/D/E,
Operation & Maintenance Manual
water-jacketed Incubator has been designed to provide a reliable controlled in
2
control
NU-4950/D/E
gas control system combining state-of-the-
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