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Pyxis EM-400 Operating Manual

Handheld in-vivo chlorophyll-a analyzer
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Rev. 1.0
Firmware version 1.10

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Summary of Contents for Pyxis EM-400

  • Page 1 Rev. 1.0 Firmware version 1.10...
  • Page 2 Pyxis Lab, Inc 120 Jeffrey Ave. Holliston, MA 0176 www.pyxis-lab.com service@pyxis-lab.com...

  • Page 3: Table Of Contents

    1 Contents General Description ................. 1 Specification..................1 Pyxis EM-400 Major Features ............1 Unpacking the Instrument ............... 2 Standard Accessories ................ 2 Optional Accessories ................. 2 Light Shield Cover ................2 Start Pyxis EM-400 ................... 3 Battery Installation ................3 Description of the Control Keys ............
  • Page 4 Confidentiality The information contained in this manual may be confidential and proprietary and is the property of Pyxis Lab Inc. Information disclosed herein shall not be used to manufacture, construct, or otherwise reproduce the goods disclosed herein. The information disclosed herein shall not be disclosed to others or made public in any manner without the express written consent of Pyxis Lab Inc.

  • Page 5: General Description

    Chlorophyll a is the main photosynthetic pigments of algae. Chlorophyll a measurement can be used to assess algal biomass. Main features include:  The EM-400 is pre-calibrated with using a live algae sample. The fluorescence chlorophyll-a measurement is automatically compensated for sample color and turbidity interference.

  • Page 6: Unpacking The Instrument

    Remove the instrument and accessories from the shipping container and inspect each item for any damage that may have occurred during shipping. Verify that all items listed on the packing slip are included. If any items are missing or damaged, please contact Pyxis Customer Service at service@pyxis-lab.com. 1.4 Standard Accessories ...

  • Page 7: Start Pyxis Em-400

    Replace the battery to resume operation of the EM-400 after the battery warning. The EM-400 will automatically turn on in the measurement mode after new battery installation. The EM-400 battery compartment, shown in Figure 2, is on the back side of the instrument. Install battery as follows: 1.

  • Page 8: Description Of The Control Keys

    2.2 Description of the Control Keys The EM-400 has three keys as shown in Figure 1. The left (<), right (>) and OK keys are used to launch an action indicated on the screen directly above the keys. Please note that the screen is not a tough screen. The labels above the keys indicate the function associated with the keys and can change according to the screen modes.

  • Page 9: Chlorophyll-A Measurement

    3 CHLOROPHYLL-A Measurement 3.1 Measurement When powered on, the EM-400 will be in the measurement (read) mode (see figure The water sample can be transferred to the measurement cell using a pipette or filled directly from a faucet, sample bottle, or sample valve.

  • Page 10: Calibration

    4 Calibration The EM-400 fluorimeter was calibrated using a laboratory cultured live algae sample. The chlorophyll-a concentration in the standard sample was determined by the standard extraction and spectrophometry procedure. Since chlorophyll-a in different algae species has different fluorescent properties, the user must calibrate EM-400 with the real sample under investigation in order to measure an absolute (versus relative) algae concentration.
  • Page 11 Press Calibration key (<). Figure 5, the first screen of the CHLOROPHYLL-A calibration appears. MEASUREMENT CHLOROPHYLL A CALIBRATION Insert DI Solution Press Zero Button Zero Slope Exit Figure 5 Press Zero labeled key (<) to set the zero point. After successful zero set, a checkmark symbol will appear next to “Press Zero Button”...
  • Page 12 Rinse the sample cell out thoroughly (twice) with the desired chlorophyll a standard and with the measurement cell near full, close the light shield. (If the screen darkens, promptly press any key to re-set the timer for auto shutdown. The key pressed does not perform its activity, only re-sets timer. The screen then re-lights.) Press the Slope labeled key (>) to set the slope of the standard desired and complete chlorophyll a calibration.

  • Page 13: Device Information And Diagnosis

    Press the diagnosis labeled key to switch to the diagnosis screen where raw measurement data are displayed (Figure 9). The information has no use for normal operation. Please provide an image of both the device information screen and the diagnosis screen when you contact Pyxis (service@pyxis-lab.com) for troubleshooting your device. MEASUREMENT MEASUREMENT...

  • Page 14: Wireless Connection

    6 Wireless Connection The Pyxis EM-400 can be connected to a smart phone or a computer via WIFI or Bluetooth for upgrading the device software. The EM-400 can be wirelessly paired with other Pyxis devices for exchanging data. In the normal operation modes, the wireless function is turned off.

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