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Bertin Technologies Cryolys Evolution User Manual

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Document reference: CLYS-800-DU002-A
Document reference: CLYS-800-DU001-A
Translated instructions from the French original version
Original Instructions
Cryolys® is a registered trademark of Bertin Technologies.
Cryolys
is a registered trademark of Bertin Technologies.
®
All rights reserved
© 2017 Bertin Technologies.
© 2017 Bertin Technologies. All rights reserved
For Research purposes only
For research use only

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Summary of Contents for Bertin Technologies Cryolys Evolution

  • Page 1 Document reference: CLYS-800-DU002-A Document reference: CLYS-800-DU001-A Translated instructions from the French original version Original Instructions Cryolys® is a registered trademark of Bertin Technologies. Cryolys is a registered trademark of Bertin Technologies. ® All rights reserved © 2017 Bertin Technologies. © 2017 Bertin Technologies. All rights reserved...
  • Page 2 Restricted User License: By purchasing or using the Product, the user accepts the terms and conditions of this License in full. The purchase or use of the Product confers on the User the non-transferable right to use the Product for Research purposes only. The term Research denotes any scientific research programs conducted directly by the User for their own purposes;...
  • Page 3 The information contained in this document is provided solely for use with this Bertin Technologies product under the terms and conditions of the Restricted User License. Bertin Technologies is not liable for any use of this information when applied to other products.

  • Page 4: Table Of Contents

    Table of Contents ............................1 Introduction........................5 Safety instructions and recommendations ........... 5 Warranty ......................9 Description of the Cryolys Evolution ..............10 ® Description of the equipment ............... 10 Specifications ....................11 Generating cold air ..................12 Installing the Cryolys Evolution ................

  • Page 5: Introduction

    Bertin Instruments. Bertin Technologies cannot be held liable for any damage or injuries caused by using this device in a manner other than that described in this manual. WWW.BERTIN-INSTRUMENTS.COM...
  • Page 6 Reference labels are attached to the device's components. Users must comply with the information printed thereon. 1.1.1 Risk of electric shock As the Cryolys Evolution is connected electrically to the rear panel of the ® Precellys Evolution, usage precautions and recommendations against electric ®...
  • Page 7 1.1.3 Risks linked to dry ice Dry ice (cardice) is made from an accumulation of carbon dioxide in a solid state with a temperature of -80°C. It sublimates in air into CO , a colorless and odorless gas. The principal risks linked to the handling of this product are asphyxia and burns.
  • Page 8 1.1.5 Special Safety Symbol Comply with the safety instructions associated with this symbol. 1.1.6 Recommendations Any use of this device other than that described in this manual can alter its external protection and damage its internal components. We recommend that you: Do not install unauthorized components or accessories;...

  • Page 9: Warranty

    - Opening, repair or modification of the device by unauthorized persons. - Alteration or removal of the label on which the device's serial number is printed. Manufacturer: Bertin Technologies Parc d’activité du Pas du Lac 10bis Avenue Ampère 78180 MONTIGNY-LEBRETONNEUX FRANCE WWW.BERTIN-INSTRUMENTS.COM...

  • Page 10: Description Of The Cryolys ® Evolution

    2 DESCRIPTION OF THE CRYOLYS EVOLUTION ® The Cryolys Evolution is a device designed to keep biological samples in the ® Precellys Evolution at a temperature between 0°C and 10°C to avoid any ® degradation linked to a temperature increase during homogenization. The Cryolys Evolution allows you to: ®...

  • Page 11: Specifications

    Main seal: ensures a good seal between the cover and the module. Diffuser Plate: this is attached to the cover and directs air over the dry ice. Cooling module: ensures uniform diffusion of cooled air inside the Precellys ® Evolution. Module cover: Encloses the cooling module.

  • Page 12: Generating Cold Air

    Weight (empty module) 1.1 kg Operating temperature 15-30°C Humidity 15-85% RH Altitude < 2,000 m Operating specifications Maximum air flow 168 m /hour Dry ice reservoir 1.8 kg Dry ice consumption Approx. 1.5 kg/30 mins Minimum dry ice volume 0.5 kg Noise level <...
  • Page 13 Temperature during homogenization without Cryolys Evolution ® Temperature during homogenization with Cryolys Evolution ® Precellys Evolution homogenization cycles ® Figure 2: Temperature inside the tubes during homogenization with and without Cryolys Evolution ® WWW.BERTIN-INSTRUMENTS.COM...

  • Page 14: Installing The Cryolys ® Evolution

    3 INSTALLING THE CRYOLYS EVOLUTION ® 3.1 Unpacking and installation It is important to check the delivered package carefully and inform your carrier of any damage. 3.1.1 Package contents The package contains the following items: One Cryolys Evolution (module + specific Precellys® lid) ®...
  • Page 15 3.1.2 Unpacking the package 1. Open the cardboard 2. Pull out the inner box 3. Open the inner box box. using the central handle. and pull out the top foam protector. 4. Check that all 5. Separate the Cryolys 6. Lift the hinge ®...
  • Page 16 CAUTION - IMPORTANT Do not hold the Cryolys Evolution by its cover ® 3.1.3 Installing the Cryolys Evolution ® Note: Make sure the Precellys® Evolution is disconnected from the power supply before installation. Required tool: N 5 Allen (hex) key 1.
  • Page 17 4. Insert the hinge 5. Gently tighten the 2 6. Connect both fan and reinforcement into the screws supplied with the temperature sensor hinge screw holes cover connectors to their respective connections at the back of the Precellys Evolution. ® 7.
  • Page 18 Figure 3: Precellys Evolution "settings" menu ® Registering your product will ensure you are informed when new software versions are made available for the Precellys Evolution. This can be done via the ® Bertin Instruments website (www.bertin-instruments.com). 1. Insert the USB stick 2.

  • Page 19: Packaging And Transport

    4. Press OK and wait 60 seconds. 5. The Precellys Evolution will restart ® automatically. 3.2 Packaging and transport Before transporting the device, you must: Check that there is absolutely no dry ice inside the module Ensure there is no humidity or, if necessary, dry any humid areas If you have to transport or ship the Cryolys Evolution, arrange and pack it by ®...

  • Page 20: Storage

    4. Detach the hinge 5. Lift the lid off the 6. Replace the module reinforcement from the Precellys Evolution. on top of the Precellys® ® hinge on the Precellys lid and place the entire ® Evolution. unit inside the foam protector and on the inner box 7.

  • Page 21: Use

    4 USE 4.1 Description of the user interface 4.1.1 Main interface Figure 4: Precellys Evolution main interface screen ® Mixer protocol settings interface: see Precellys Evolution user manual ® (02520-800-DU003-B). Cooling protocol settings’ interface: allows you to set the various parameters of the Cryolys Evolution.

  • Page 22: Starting Temperature-Controlled Homogenization

    Operating temperature selection field: allows you to set the temperature between 0 and 10°C Operating mode selection field: allows you to choose manual or automatic mode Note: for information about navigating the Precellys Evolution from the screen interface, ® please refer to the Precellys Evolution user manual (02520-800-DU003-B).
  • Page 23 4.2.2 Prepare the samples Place the samples into the Precellys tubes and add the required buffer if necessary Note: Bertin Instruments recommends the use of the Precellys Lysing Kits. ® Close the tubes and arrange them ad hoc in the tube holder Note: Bertin Instruments guarantees the compatibility of all Precellys consumables ®...
  • Page 24 Program the homogenization parameters via the dedicated interface: volume, speed, number & duration of cycle and pause Program cooling parameters via the dedicated interface: ON/OFF, temperature and mode Note: You can use a saved protocol if the Cryolys Evolution module activation field is set ®...
  • Page 25 When the chosen temperature is reached, the following message appears: "Temp. is reached." Open the Precellys® lid Place the tubes and the tube holder (fitted with the plate) into the Precellys Evolution (refer to the Precellys Evolution user manual 02520- ®...

  • Page 26: Cleaning And Decontamination

    5 CLEANING AND DECONTAMINATION 5.1 Recommendations For safety reasons, and to avoid damaging the instrument, follow these instructions: Do not spray anything directly onto the device, particularly on the electrical connectors. Clean the instrument when it is unplugged. Do not use a sponge-scourer to avoid damage to the device. Do not use an airborne decontamination process.

  • Page 27: Support And Documentation

    6 SUPPORT AND DOCUMENTATION 6.1 Frequently asked questions Question Answer Can the Cryolys Evolution fall off when We recommend separating the cooling ® I open the cover of the Precellys module containing dry ice from the ® Evolution? cover when operating the cover Can I use any type of ice (ice cubes, The Cryolys Evolution must absolutely...

  • Page 28: Troubleshooting

    6.2 Troubleshooting Problem Possible cause Action(s) to be performed encountered Module is badly Reposition the module on the positioned Precellys® lid. Message 1. Check that the interface appears: "No board connector is Cryolys Evolution ® plugged in has been Bad connection 2.

  • Page 29: Support

    This equipment is subject to selective collection in accordance with the European directive on waste electrical and electronic equipment (WEEE) 2012/19/EU. Bertin Technologies is responsible for treatment of WEEE for products sold in France (via RECYLUM). For other countries, the importer is responsible for treatment and this waste treatment is dependent on current regulations.

  • Page 30: Appendix: Example Of Ce Declaration

    7 APPENDIX: EXAMPLE OF CE DECLARATION WWW.BERTIN-INSTRUMENTS.COM...

  • Page 31: Appendix: Electrical Diagram

    APPENDIX: ELECTRICAL DIAGRAM WWW.BERTIN-INSTRUMENTS.COM...
  • Page 32 WWW.BERTIN-INSTRUMENTS.COM...

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