Histogram Statistics; Linear Region Statistics; Log Region Statistics - Beckman Coulter Navios Instructions For Use Manual

Histogram Statistics

Linear Region Statistics

For linear signals, statistics for histogram regions are calculated as follows:
total =
---------------------------------------------------------------------------------------------------------- -
Total number of events in the gated display
Number of cells in region
total =
----------------------------------------------------------------------------- -
Total number of cells in the file
Number = Number of cells in the region
Mode = Intensity containing the largest number of cells within the region
Total is expressed as a percent (%).
Where d = Standard deviation
CV = Coefficient of Variation (expressed in % CV)
M = High region marker channel
H
M = Low region marker channel
L
C = Raw digital channel number
n
C = Channel count
ch
N = Integral of all counts between & including marker channels
= Mean channel number (rounded to nearest whole channel)
For Mean and SD, the summations are performed over all the channels that lie within the region.
Mean = median intensity
Median intensity = the smallest intensity such that sum (intensity) 
Sum (intensity) = sum of events from the lower edge of the region to the intensity
Half Peak CV
Where FWHM=the Full Width Half Max value of a Normal or Gaussian peak.

Log Region Statistics

The method of geometric calculations of Means depends on your
773232AH
Number of events in region
×
100
(
sum median intensity
+ 0.5 –
------------------------------------------------------------------------------- -
count in median intensity
FWHM
=
------------------- -
2.354
×
100
) area
–
---------- -
2
Advanced Statistics
Operation Principles
Plot Display
area
---------- -
2
Configuration.
3-15
3