Signal Processing; Voltage Pulse Signals; Peak Signal; Two Laser 8 Color Filter Block Configuration - Beckman Coulter Navios Instructions For Use Manual

Operation Principles

Signal Processing

Figure 3.5 Two Laser 8 Color Filter Block Configuration
525 BP 40
550 DC SP
595 DC SP
Figure 3.6 Three Laser 10 Color Filter Block Configuration
Signal Processing

Voltage Pulse Signals

The Cytometer has up to twelve sensors (FS, SS,FL1-FL10), each generating a voltage pulse signal as
each cell passes through the laser beam. A voltage pulse signal is proportional to the intensity of
light the sensor received. The Cytometer electronics amplify, condition, integrate, and analyze
these pulses.

Peak Signal

Figure 3.7 shows how a peak voltage pulse signal forms as a cell crosses the laser beam.
• Part I of
3-6
755 LP
655 DC SP 620 BP 30
550 DC SP 655 DC SP
595 DC SP 730 DC SP
575 BP 30 730 DC SP
Figure 3.7 shows when the cell enters the laser beam and some light is scattered.
660 BP 20
710 DC SP
755 LP
710 DC SP
750 DC SP
695 BP 30/ 725 BP 20
675 BP 20
750 DC SP
773232AH