Fluorescent Light; Side Scatter Collection; Two Laser, 6 Color Filter Block Configuration - Beckman Coulter Navios Instructions For Use Manual

Figure 3.3 Side Scatter Collection
b
c
d
1. Violet laser beam
2. Blue laser beam

Fluorescent Light

Band pass and Long pass filters are used to transmit color bands. The color bands are designed to
measure fluorescence light from the fluorochromes such as FITC, PE, ECD, PC5, PC5.5, PC7, APC, APC
AlexaFluor700, APC AlexaFluor750, Pacific Blue and Krome Orange (with PMT and violet laser
upgrades installed) that are excited by illumination from the lasers. Dichroic filters are used to
reflect colors. Positions of the dichroic filters have been efficiently designed to reduce the number
of optical surfaces fluorescence light must pass to reach the photo sensors. Their locations relative
to the optical axis have also been optimized for light to pass symmetrically through each filter. You
can individually interchange the optical filters. There is no need to realign the optical system when
the filters are changed.
Figure 3.4 Two Laser, 6 Color Filter Block Configuration
525 BP 40
550 DC SP
595 DC SP
773232AH
3. Red laser beam
4. Side scatter collected through fiber optic cable
mounted on the right side of flow cell
755 LP
655 DC SP 620 BP 30 710 DC SP
550 DC SP 655 DC SP
595 DC SP 730 DC SP
575 BP 30 730 DC SP
Light Collection, Separation And Measurement
e
660 BP 20
710 DC SP
695 BP 30/
675 BP 20
Operation Principles 3
3-5