Setting A Stop Counter; Use Stop Condition - Beckman Coulter Navios Instructions For Use Manual

Using Navios Software
Plots Menu
In normal blood, where lymphocytes account for about 30% of the white cell count, we have no
problem with the above setup. We can predict with a normal sample that the total count from a
5,000 cell lymphocyte count is around 5,000 x 100/30 or 16,666 events total.
If the sample is from a lymphopenic patient, this could be a problem. A 5,000 lymphocyte count may
not be achieved before the sample tube is empty or RAM memory is full.
It is normal under these circumstances to set two counters, one for the stop count and one for the
maximum permissible count, that is, the maximum total event count. If a stop count is used, it is
recommended that the stop count be set to a large number, for example, 100,000 events. Thus, with
a lymphopenic patient, if the lymphocytes are less than 5% of the white cell count, an acquisition
would terminate on the total event count of 100,000 before a 5,000 cell lymphocyte count could be
achieved.
NOTE
If a Stop condition is reached while you are adjusting voltages and gains, acquisition is not restarted
and the data plots are not refreshed. You can avoid this situation by making all cytosetting adjustments
while in

Setting a Stop Counter

NOTE
If a stop count is used on a plot that contains an AutoGate region, the stop count is not exact.
1
H ighlight the plot on which you wish to set a stop counter.
2
Plots > Acquisition Save and Stops
box.

Use Stop Condition

Maximum Events
Save Histogram Data in FCS Format
NOTE
10-112
Setup Mode.
You cannot make any changes on this screen when running a
command to view the Acquisition Plot Set-up dialog
locked protocol.
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