How Is Prism Created; Prism Dividers; Setting A Single-Parameter Prism Divider - Beckman Coulter Navios Instructions For Use Manual

How is Prism Created?

In single parameter analysis of surface marker data, one or two regions are normally set to measure
both Negative and Positive cells. We can then look at a second parameter and calculate the 2
phenotypes from parameter-1 positive populations and the two phenotypes from parameter-1
negative populations (four phenotypes in total).
We can repeat this with 3, 4 or more parameters.
The Prism Divider can be used instead to mark the dividing line between the Negative and Positive
cells.

Prism Dividers

A Prism is created by setting one or more prism dividers. There are three ways of setting prism
dividers, two of them involve graphical positioning (single and dual prism dividers). The third uses
Analysis > View/Modify Prism
divider on one plot is replicated on any other plot using that parameter.
Each new parameter that has a prism divider set on it multiplies the total number of phenotypes by
2.
Only one divider can be set per parameter.

Setting a Single-Parameter Prism Divider

Single parameter prism dividers are set by placing a
Single-parameter histogram in focus and selecting
. The divider can then be set by clicking on the
selected plot, positioning the divider, and then clicking
once more to fix the divider position.
773232AH
that can set the divider numerically on any divider. Any changes to the
Using Navios Software 10
Plots Menu
10-105