Olympus U-UCD8A Instructions Manual
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Olympus U-UCD8A Instructions Manual

Motorized universal condenser
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INSTRUCTIONS
U-UCD8A
MOTORIZED UNIVERSAL CONDENSER
This instruction manual is for the Olympus Motorized Universal Condenser Model U-UCD8A. To ensure the
safety, obtain optimum performance and to familiarize yourself fully with the use of this condenser, we
recommend that you study this manual thoroughly before operating the microscope system. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 3 5 5

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Summary of Contents for Olympus U-UCD8A

  • Page 1 U-UCD8A MOTORIZED UNIVERSAL CONDENSER This instruction manual is for the Olympus Motorized Universal Condenser Model U-UCD8A. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this condenser, we recommend that you study this manual thoroughly before operating the microscope system. Retain this instruction manual in an easily accessible place near the work desk for future reference.
  • Page 2 Printed on 100% recycled paper with soy ink.

  • Page 3: Table Of Contents

    U-UCD8A CONTENTS IMPORTANT — Be sure to read this section for safe use of the equipment. — 1 SYSTEM DIAGRAM 2 NOMENCLATURE 3 VARIOUS MICROSCOPY PROCEDURES 6-18 3-1 Preparation ..................................................6 3-2 Brightfield Observation (BF) ......................................6-8 3-3 Phase Contrast Observation (PH) ..................................9-10 3-4 Nomarski Differential Interference Contrast Observation (DIC) ................

  • Page 4: Important - Be Sure To Read This Section For Safe Use Of The Equipment

    IMPORTANT · The U-UCD8A is a motorized universal condenser that can be used for advanced research applications by combining it with the BX-UCB control box and U-HSTR2 hand switch (interlocking with other motor- ized modules is also available by running the BX2 software on a PC).
  • Page 5 U-UCD8A 10. An intermediate tube or sliders may be necessary depending on the method of observation. 11. In brightfield observation, engage the optical elements (small) other than 1, 2 and 3 in the light path. The field of view may be cut off if optical elements 1, 2 and/or 3 are used.

  • Page 6: System Diagram

    SYSTEM DIAGRAM }For the connection of connection cables, refer to the instruction manual for the BX-UCB control box. Motorized Universal Condenser Control Box U-UCD8A BX-UCB Microscope BX/BX2 series Motorized Transmitted Light Filter Wheel Hand Switch U-FWT* U-HSTR2 * Necessary in DIC and simple polarized light observation using polarizer (U-FW32PO).

  • Page 7: Nomenclature

    U-UCD8A NOMENCLATURE Connector To be connected to the BX-UCB. Top lens (Page 23) The U-TLD dry type top lens Turret cover clamping screw (Page 30) (provided as standard) may be exchanged with the U-TLO oil immersion type top lens (optional).
  • Page 8 Optical Elements (For the applicable objectives, see pages 25 to 29.) Phase Contrast Ring (Small) DIC Prism (Small) DIC Prism (Large) U-DIC10S U-PH1S Other DIC prism than the U-DP10S U-PH2S U-DIC10S/U-DP10S. U-PH3S Darkfield ring Phase contrast ring (Large) U-DFA U-PH3 ·...

  • Page 9: Various Microscopy Procedures

    U-UCD8A VARIOUS MICROSCOPY PROCEDURES 3-1 Preparation · If the top lens and optical elements have not been assembled yet, first read Chapter 6, ASSEMBLY” (pages 23 to 35). · Set the main switch of the BX-UCB control box to “ I ” (ON) to make the microscope ready for observation.
  • Page 10 }When a transmitted light DIC slider (U-DICT, U-DICTS, etc.) is used in combination, pull out the slider until it clicks in place to disengage the slider from the light path. }When a reflected light analyzer (U-AN) is used in combination, pull out the analyzer until it clicks in place to disengage the analyzer from the light path.
  • Page 11 U-UCD8A Field Iris Diaphragm Aperture iris 70-80% diaphragm {The field iris diaphragm controls the size of the illuminated 30-20% image area. By stopping down the field iris diaphragm, in accordance with the objective in use, until its image circumscribes the field of view, stray light can be reduced, which in turn increases the definition and contrast of the image.

  • Page 12: Phase Contrast Observation (Ph)

    3-3 Phase Contrast Observation (PH) Applicable objective power Power 100X Phase Contrast (PH) ¦* ¦ ¦ ¦ ¦ Top lens IN * With the super-widefield observation (FN 26.5), flare may be observed in the peripheral areas of the field of view.
  • Page 13 U-UCD8A 6. Rotate the upper section of the U-CT30 centering telescope and bring the bright ring (condenser ring slit) and dark ring (objective phase plate) into focus. 7. Use the optical element centering knob to center the phase contrast ring so that the bright ring overlaps the dark ring within the field of view.

  • Page 14: Nomarski Differential Interference Contrast Observation (Dic)

    3-4 Nomarski Differential Interference Contrast Observation (DIC) Applicable objective power Power 100X ¦ ¦ ¦ ¦ ¦ Nomarski Differential Interference Contrast (DIC) Top lens IN To perform Nomarski DIC observation, the transmitted light DIC slider and an analyzer (U-ANT or U-AN) are required.
  • Page 15 U-UCD8A Installation using U-DICTS, U-DICTHC or U-DICTHR Clamping knob** Analyzer (U-AN) Prism position switch knob Pushed in: BFP1 To be inserted into the mount on the reflected Pulled out: NORMAL light illuminator DIC-compatible revolving nosepiece Analyzer for (U-D6RE, U-D5BDRE, etc.)
  • Page 16 1. Adjust the polarizer in accordance with the following procedure. a) Engage the transmitted light DIC slider in the light path and tighten the clamping knob. b) Engage the U-ANT or U-AN analyzer in the light path. 2. Select the BF brightfield observation light path (with no optical element engaged). 3.
  • Page 17 U-UCD8A Operation using U-DICT without U-ANT a) Pull out the transmitted light DIC slider to disengage it from the light path. b) While looking into the eyepiece, loosen the clamping screw of the U-FWT filter wheel and rotate the U-FWT to the position where the field of view is perfectly dark.
  • Page 18 9. Rotate the prism control knob of the DIC slider to adjust the contrast of the background color as discussed below. a) Rotate the prism control knob of the DIC slider to obtain the background interference color that can achieve the maximum contrast according to the specimen under observation.

  • Page 19: Darkfield Observation (Df)

    U-UCD8A 3-5 Darkfield Observation (DF) Applicable objective power Power 100X Darkfield (DF) ¦* ¦** ¦** ¦** ¦** Top lens IN * With the super-widefield observation (FN 26.5), flare may be observed in the peripheral areas of the field of view.

  • Page 20: Simple Polarized Light Observation (Kpo)

    6. Insert the eyepiece into the eyepiece sleeve and look at the darkfield image. Repeat centering until the optimal darkfield effect is obtained. 7. Move the condenser up or down until uniform darkfield illumination is attained. 8. Open the field iris diaphragm to the extent that even brightness is attained. # Keep eyes away from the eyepiece while changing the objective during darkfield observation or chang- ing from darkfield observation to another observation mode.
  • Page 21 U-UCD8A 1. Select the BF brightfield light path (with no optical element engaged). 2. Engage the U-FW32PO polarizer on the U-FWT filter wheel in the light path. 3. Mount the objective to be used in the revolving nosepiece, and rotate the nosepiece to swing in the objective.

  • Page 22: Troubleshooting Guide

    Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Problem...
  • Page 23 U-UCD8A Problem Cause Remedy Page c. Image glares and resolution Aperture iris diaphragm is stopped down Open aperture iris diaphragm to proper is low under brightfield obser- excessively. diameter. vation. Top lens is swung out. Swing top lens into light path when 10X –...
  • Page 24 Problem Cause Remedy Page g. No interference color appears Polarizer is not inserted in light path. Engage polarizer in light path. during Nomarski observation. Analyzer is not inserted in light path. Engage analyzer in light path. Engage DIC prism by rotating turret. DIC prism is not inserted in light path.

  • Page 25: Specifications

    U-UCD8A SPECIFICATIONS Specifications Item Dry type top lens (U-TLD) Immersion type top lens (U-TLO) Applicable microscopes BX or BX2 series Transmitted light (brightfield, darkfield, phase Transmitted light (brightfield, DIC) Applicable microscopy contrast, DIC, polarized light) Type Achromat-Aplanat, motorized swing-out type top lens (Top lens interchangeable) 1.4 (top lens in) when immersed in oil, 0.2 (top...

  • Page 26: Assembly

    ASSEMBLY The motorized parts may be damaged if you attempt to operate them manually with an excessive force. Connect them according to the instruction manual of the BX-UCB control box, set the main switch to “ I ” (ON) and control them from the U-HSTR2 hand switch. When operating a switch, be careful not to approach your hand or finger to the condenser to prevent injury.

  • Page 27: Mounting The Optical Elements

    U-UCD8A 6-2 Mounting the Optical Elements Optical Element Mounting Positions (Fig. 6) 1, 3: The U-DIC10S or U-DP10S optical element can be mounted. When not in use, any one of the U-PH1S, U-PH2S and U- PH3S can be mounted here.
  • Page 28 Optical Elements and Compatible Objectives Phase contrast (PH) and darkfield (DF) observations: When the U-TLD top lens is used UIS2 Series Observation Optical Element Applicable Objectives U-PH1S PlanN10XPh, PlanN20XPh, UPlanFLN10XPh, UPlanFLN20XPh U-PH2S PlanN40XPh, UPlanFLN40XPh PlanN100XOPh, UPlanFLN60XOPh, UPlanFLN100XOPh U-PH3S, U-PH3 PlanN10X, PlanN20X, PlanN40X, PlanN50XOI, UPlanFLN10X, UPlanFLN20X, UPlanFLN60XOI, U-DFA UPlanFLN100XOI, UPlanSApo10X UIS Series...
  • Page 29 U-UCD8A UIS2 Series DIC observation (U-DICT/DICTS) (Note) For the U-DICTHC and U-DICTHR, see next page. DIC Slider U-DICT Shift type U-DICTS Top Lens U-TLD U-TLO U-TLD U-TLO UPlanFLN U-DIC10, U-DIC10S U-DIC10, U-DIC10S U-DIC20 U-ODIC20 U-DIC20 U-ODIC20 U-DIC40 U-ODIC40 U-DIC40 U-ODIC40...
  • Page 30 DIC observation (U-DICTHC/DICTHR) High-contrast type High-resolution type DIC Slider U-DICTHC U-DICTHR U-TLO Top Lens U-TLD U-TLD (for VEC/DIC) UPlanFLN U-DIC10HC U-DIC10HR U-DIC20HC U-DIC20HR U-DIC40HC U-DIC40HR 40XO U-DIC40HC U-DIC40HR U-DIC60HC U-DIC60HR U-ODIC60HR 60XOI 100XO U-DIC100HC U-DIC100HR U-ODIC100HR 100XOI UPlanSApo U-DIC10HC U-DIC10HR U-DIC20HC U-DIC20HR U-DIC40HC...
  • Page 31 U-UCD8A UIS Series (Note) Usable regardless of the model number (3, 2 or none). DIC observation (U-DICT/DICTS) (Note) For the U-DICTHC and U-DICTHR, see next page. DIC Slider U-DICT Shift type U-DICTS Top Lens U-TLD U-TLO U-TLD U-TLO UPlanFI U-DP10, U-DP10S...
  • Page 32 DIC observation (U-DICTHC/DICTHR) High-contrast type High-resolution type DIC Slider U-DICTHC U-DICTHR U-TLO Top Lens U-TLD U-TLD (for VEC/DIC) UPlanFI U-DP10HR U-DP20HC U-DP20HR U-DP40HC U-DP40HR 60XOI3 U-DPO60HC U-DPO60HR U-ODPO60HR 100XO3 U-DP100HC U-DP100HR U-ODP100HR 100XOI3 UPlanApo U-DP10HR 10XW3 10XO3 U-DPA20HR 20XO3 U-DPA40HR 60XW3 U-DPO60HC U-DPO60HR...
  • Page 33 U-UCD8A Mounting the Phase Contrast ² (Figs. 7 - 9) Ring or Darkfield Ring 1. Swing in the top lens by motorized operation. ³ 2. Using the Allen screwdriver provided with the condenser, loosen the turret cover clamping screw @ and remove the turret cover ².
  • Page 34 5. Insert the phase contrast ring or darkfield ring in the aperture Spring | Optical of the turret as far as it will go. element Slightly depress the spring | provided inside the turret with the side of ring while inserting. # Be careful not to push the ring sit plate inside the element frame.
  • Page 35 U-UCD8A Mounting the DIC Prism (Figs. 10 & 11) }The mounting method is variable between the mounting posi- tions with centering mechanism (1, 3, 4, 8) and those without centering mechanism (5, 6, 7). When the mounting position is 1, 3, 4 or 8: The DIC prism can be mounted in the same way as a phase contrast ring.
  • Page 36 When the mounting position is 5, 6 or 7: }To clamp the DIC prism, do not use the built-in optical ele- ment centering screw but use the provided Allen wrench ² which is designed exclusively for this purpose. 1. Engage the position to be used in the light path by motorized operation, loosen the position clamping screw by inserting the Allen wrench ²...

  • Page 37: Mounting The Condenser

    U-UCD8A 6-3 Mounting the Condenser (Fig. 12) Also refer to the instruction manual for the microscope in use. Attach the condenser to the condenser holder in accordance with the following procedure. 1. Press the stage UP botton @ to raise the stage to a height where it does not hit the objective.

  • Page 38: Centering The Condenser

    6-4 Centering the Condenser (Fig. 13) Also refer to the instruction manual for the microscope in use. Center the condenser in accordance with the following proce- Field iris diaphragm image dure. Eyepiece field of view 1. Select the BF (brightfield) observation light path (with no opti- cal element engaged).
  • Page 40 Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan Wendenstrasse 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London, EC1Y OTX, U.K. 31 Gilby Road, Mount Waverley, VIC., 3149, Australia 5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.

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