Sliding Door; Needle Holder - BMG Labtech PHERAstar FS Operating Manual

BMG LABTECH
Cleaning with acid / base (best procedure if cells are used in the syringe)
1. Fill the syringe with 0.1M NaOH and leave it in the syringe for 10 minutes.
2. Flush the syringe a minimum of 10 times with distilled water.
3. Fill the syringe with 0.1M HCl, and leave the solution in the syringe for 10 minutes.
4. Flush the syringe a minimum of 10 times with distilled water.

4.6.2 Sliding Door

The Sliding Door can be opened by pressing onto the two ends of the handle and sliding it upwards. The
door will stay at the position it is released. The PHERAstar FS can not start a test run if the sliding door is in
the open position. Thus always close the sliding door before starting a test run.

4.6.3 Needle Holder

The needle holder is an S-shaped device and houses one or two needles connected to the respective
reagent injectors (figure 25). The arrangement of the needles and the architecture of the needle holder itself
allow simultaneous injection and measurement for all detection modes except absorbance. The tubing
coming from the syringe barrels are assembled together in a magnetic clamp to minimize dead volume and
to guide them to the needle holder. The magnetic clamp can easily be removed by gently pulling it away from
the instrument. In order to execute external priming, to exchange the needle holder or to clean the needles it
is necessary to first pull away the magnetic clamp from the reader. The magnetic clamp is also equipped
with a sensor which prevents the PHERAstar FS from executing a test run when the magnetic clamp and the
needle holder are not properly installed.
Magnetic Clamp
Needle holder with
needles
Figure 25: Needle holder assembly
The needle holder slides easily in and out off its reference position. Note that the tubing assembly, the
magnetic tube clamp, and the needle holder always come as one unit. The waste container for internal
priming comes as a unit but can be taken apart for cleaning as shown in figure 26.
Two versions of the needle holder assembly are available. Needle holder A1 incorporates 2 needles and is
used for the 96/384 square well plate format. Needle holder A2 has 1 needle and is used for the 96/384
round well plate format. Each version is recognized in the Control software by a sensor placed on the inside
of the magnetic tube clamp.
2009-09-22
Connection to the valves
of the syringes
0471B0001A
PHERAstar FS Operating Manual
Figure 26: Waste container
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