Related Manuals for BMG Labtech FLUOstar Omega
Summary of Contents for BMG Labtech FLUOstar Omega
- Page 1 FLUOstar Omega LUMIstar Omega POLARstar Omega SPECTROstar Omega Operating Manual Revision G...
- Page 2 Germany www.bmglabtech.com support@bmglabtech.com +49-781-969680 Copyright 2007-2014 BMG LABTECH. All rights reserved. All BMG LABTECH brand and product names are trademarks of BMG LABTECH. Other brand and product names are trademarks or registered trademarks of their respective holders. 2/29 0415B0001G...
- Page 3 Although these instructions were carefully written and checked, we cannot accept responsibility for problems encountered when using this manual. Suggestions for improving this manual will be gratefully accepted. BMG LABTECH reserves the right to change or update this manual at any time. The Revision Number is stated at the bottom of every page.
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Page 4: Table Of Contents
Omega Operating Manual BMG LABTECH TABLE OF CONTENTS TECHNICAL SPECIFICATIONS SAFETY INFORMATION ESCRIPTION OF ARNINGS ENERAL NFORMATION NVIRONMENTAL AFETY TANDARDS LECTRICAL AFETY HOTOBIOLOGICAL AFETY HEMICAL AND IOLOGICAL AFETY LEANING AND NSTRUMENT ISINFECTION INSTALLATION RANSPORT OFTWARE NSTALLATION OWER AND OMMUNICATION ONNECTIONS... -
Page 5: Technical Specifications
BMG LABTECH Omega Operating Manual 1 Technical Specifications Measurement principles FLUOstar Omega: - Fluorescence intensity - Time-resolved fluorescence – TR-FRET (opt.) - Luminescence (opt.) - UV/VIS absorbance spectra ® ® - AlphaScreen / AlphaLISA (opt.) - Upgradeable to POLARstar Omega... - Page 6 Omega Operating Manual BMG LABTECH Luminescence - Limit of detection 20 amol / well ATP - Spectral range 240 ... 740 nm - Dynamic range: 9 decades Fluorescence polarization - Limit of detection: <5 mP SD at 1 nM fluorescein - Spectral range: (ex.
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Page 7: Safety Information
Prior to turning on the instrument the first time, let the instrument adapt to room temperature for at least 3 h to avoid condensation which can cause a short circuit. BMG LABTECH will void the warranty if damage occurs to electrical and/or mechanical parts in cases where the instrument was turned on before the recommended accommodation time. -
Page 8: Environmental Safety Standards
Omega Operating Manual BMG LABTECH 2.3 Environmental Safety Standards The environmental safety standards for operation under norm IEC 61010-1 are met under the following conditions: • Indoor use (adhere to the Occupational Exposure Limit Values for ECM, UPS, vibration, and sunlight when setting up the instrument in the laboratory) •... -
Page 9: Chemical And Biological Safety
Omega Operating Manual A measurement run is indicated by a blinking green LED on the instrument. The following light source is incorporated and encapsulated in the BMG LABTECH ‘Omega’ microplate reader instrumentation: Xenon high power flash lamp – emits ultraviolet light during operation at levels that could injure the eyes or skin. - Page 10 Omega Operating Manual BMG LABTECH Disinfection Steps 1. Disconnect the instrument from the main power supply. 2. Remove the USB cable from the connector. 3. Clean all outside surfaces of the instrument carefully with cotton wool, which has been soaked in disinfecting solution.
- Page 11 BMG LABTECH Omega Operating Manual Disinfection Certification This instrument and its inventory have never been in contact with any dangerous biological material, or if so, the instrument and its inventory have been disinfected according to the instructions of the operating manual of the instrument.
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Page 12: Installation
When unpacking the instrument, please check to ensure that all of the following parts are included: • FLUOstar Omega or LUMIstar Omega or POLARstar Omega or SPECTROstar Omega reader • Control and MARS Data Analysis software (CD ROM in a cover inside this manual) •... -
Page 13: Software Installation
USB Communication Connection Connect the USB cable to the FLUOstar Omega (or POLARstar Omega or SPECTROstar or LUMIstar Omega) and to the USB port on the PC. Please connect the reader directly to your PC and do not use a USB-hub. -
Page 14: Instrument Overview
Instrument turned on/busy light Plate carrier Figure 4: FLUOstar Omega A constant green light means the instrument is turned on. A flashing green light means the instrument is busy (e.g. performing a measurement, plate in/out, priming, etc.). A faster flashing green light (5 flashes per second) means an error has occurred. - Page 15 Measurement Injection needle holder Absorbance optic Bottom optic head position Figure 6: FLUOstar Omega & POLARstar Omega top view of reagent box with spectrometer based absorbance and 2 reagent injectors; no top optic is installed 2014-05-16 0415B0001G 15/29...
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Page 16: Description Of Components
Omega Operating Manual BMG LABTECH 5 Description of Components 5.1 Optics All readers are equipped with standard UV/Vis optics for top reading. For the Omega, there are different top reading optics available for fluorescence intensity, fluorescence polarization, luminescence, absorbance and time-resolved fluorescence. -
Page 17: Fluorescence Intensity Optics
BMG LABTECH Omega Operating Manual 5.2.1 Fluorescence Intensity Optics The fluorescence intensity and time-resolved fluorescence light guides are silver in color, liquid-filled and come connected to the excitation and emission positioning wheels. The yellow marked light guide routes the excitation light to the sample while the blue marked light guide routes the emission light to the detector. -
Page 18: Fluorescence Polarization Optics
(PMT 1) and the center light guide into the lower emission position (PMT 2). Only the POLARstar Omega can measure fluorescence polarization. It is possible to upgrade the FLUOstar Omega to a POLARstar Omega. Regarding filters for fluorescence polarization see chapter 5.3.3. Figure 12: fluorescence polarization optic 5.2.4... -
Page 19: Absorbance Optics
BMG LABTECH Omega Operating Manual 5.2.6 Absorbance Optics This optic can only be used with readers with PMT based absorbance, not with spectrometer based absorbance. There is an optimized optic for absorbance mode available (see figure 15). This optimized absorbance optic can be used for plate formats up to 384-well. The light guide connects to the red-marked excitation-positioning wheel. -
Page 20: Advanced Optic Head For Trf / Tr-Fret
2. Make sure that the firmware of your Omega reader is at least v.1.12. The firmware version can be read by opening the Control software and clicking on the reader icon in the upper right corner. Contact BMG LABTECH in case you need an update of the reader firmware (support@bmglabtech.com). - Page 21 BMG LABTECH Omega Operating Manual 1) Open the lid of the reagent box of the FLUOstar/POLARstar Omega. Take the body of the TRF-optic head in the left hand, the light guides in your right hand and feed them through underneath the already installed excitation light guides.
- Page 22 Omega Operating Manual BMG LABTECH Figure 22: Schematic of the Quick-Fix holder (left) and actual image of the TRF-optic head in the instrument (right). Connect the excitation light guide (yellow) to the matching pin hole (yellow) of the Excitation Positioning Wheel.
- Page 23 6) Double check the layout to make sure well B2 is selected. If you have dispensed your assay solution into a different well, change the layout accordingly. 7) Set the Gain to 2300 (for white microplates) or 2500 (for black microplates). In general, BMG LABTECH recommends white plates and 200 flashes for best performance.
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Page 24: Filters
BMG LABTECH 5.3 Filters In the FLUOstar Omega, 4 excitation and 4 emission filters are factory installed; in the POLARstar Omega, 4 excitation and 5 emission filters are factory installed. (Filter selection varies with instrument configuration. If your unit is equipped with luminescence, then a lens will be installed. The PMT based absorbance option will also add filters.) - Page 25 BMG LABTECH Omega Operating Manual The excitation filters and the emission filters are located in their respective filter wheels behind the filter wheel cover (figure 28 to figure 30). To access the filters, first remove the light guides. The filter wheel cover can then be removed by loosening the 4 thumbscrews (figure 29).
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Page 26: Fluorescence Filters
Omega Operating Manual BMG LABTECH Next to the axle in the center of the housing is a small positioning pin. This pin must fit into one of the holes on the back of the filter wheel. Replace the filter wheel on the axle and push it into position (the axle should stick out 3mm). -
Page 27: Spacers
BMG LABTECH Omega Operating Manual 5.4 Spacers The Omega readers are designed for most microplate formats. The height of some microplates exceeds the space allowed under the optic. The minimum space between the optic and microplate should be 1.5 mm. -
Page 28: Reagent Injectors
Omega Operating Manual BMG LABTECH 5.5 Reagent Injectors The Omega readers can be equipped with up to 2 reagent injectors (figure 34). Figure 34: Reagent injectors When the reagent injector(s) are not in use, the needle(s) can be placed in the needle holder (figure 6). -
Page 29: Fan Cover
BMG LABTECH Omega Operating Manual 5.6 Fan Cover The Fan Cover is optional. It allows soaking ambient air into the instrument. The airflow is from the front to the back of the reader. The fan ventilating capability has the following advantages: •...
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