Description Of Optic - BMG Labtech PHERAstar FS Operating Manual

BMG LABTECH
4.5 Description of Optic Modules
There are single channel optic modules as well as dual channel optic modules for various detection
technologies.
Depending on the type of optic module, it can consist of filters, dichroic mirrors, beam splitters and
polarization film. Emission channel A is always active (whatever measurement principle is in use) and
Emission channel B is active when dual emission detection is used (for instance as in fluorescence
®
polarization, HTRF and dual luminescence assays).
The optic module is an important part of the PHERAstar FS and all parts used in the optic module are
carefully selected and accurately aligned and tested for optimal performance.
4.5.1 Fluorescence Intensity and TRF Optic Module
Standard fluorescence intensity optic modules are available in an almost unlimited variety of different
configurations (contact sales rep with your wishes). All standard fluorescence intensity optics are configured
as can be seen in figure 18.
Figure 18: Optic module for single
channel fluorescence intensity
The configuration of the filters can be freely chosen from a long list of optimized filters available.
4.5.2 Fluorescence Polarization Optic Module
The fluorescence polarization optic module consists of many parts, all specially selected and aligned for the
best performance. Fluorescence polarization optic modules (figure 19) can be customized to fit any
polarization assay, also at wavelengths other than the standard excitation 485 nm and emission at 520 nm
and 520 nm.
Figure 19: Optic module for fluorescence polarization
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