Reagent Injectors; Reagent Injection - BMG Labtech PHERAstar FS Operating Manual

Multifunctional microplate reader
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PHERAstar FS Operating Manual
4.6 Reagent Injection System
The PHERAstar FS is equipped with an internal on board reagent injection system located on the right side
of the instrument (figure 24). Use the sliding door to open, close, and to gain access to the reagent injection
system:
Needle Holder
Waste
Container
Figure 24: Overview of the PHERAstar FS Reagent Injection System
The reagent needles are made of stainless steel, the tubing and valve housing are made of Teflon and Kel-
F, and the syringe barrel is made of glass. All reagent injector materials are among the most chemical
resistant materials that are available.
Note:
Before using a pump in a test run, you need to prime the pump. This can be done using the Control
software (menu command 'Measure | Prime'). You can also prime the pumps without using the
software. When you open the reagent door, you will see two buttons for priming. Refer to chapter
4.6.5 for a detailed description of the priming procedure.

4.6.1 Reagent Injectors

In order to retain optimal performance, it is recommended to follow these guidelines:
Do not use the syringes more than two cycles without liquid.
After each use flush the syringes thoroughly with distilled water.
If the plunger is removed from the syringe barrel, it should be wiped with ethanol before replacing.
Syringes should be cleaned each week using one of the following procedures:
To remove cellular debris and viscous solutions from the syringe barrel:
Take off the syringe barrel and rinse it with distilled water. It may be useful to use the wire syringe cleaners
(can be found in the service box) to scrape particles off the walls.
Cleaning with weak detergent or 10% bleach
1. Fill the syringe with a weak detergent or 10% bleach solution
2. Leave the solution in the syringe for 30 minutes
3. Flush the syringe a minimum of 10 times with distilled water
18/26
Magnetic
Clamp
Priming Buttons
0471B0001A
BMG LABTECH
Sliding Door
Reagent
Injectors
Holder for
Compound
Vessels
2009-09-22

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