Connection Of Co 2 Gas Cylinder - Sanyo MCO-5M Instruction Manual

BEFORE COMMENCING OPERATION
Connection of CO gas cylinder
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WARNING
Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are
connected correctly and are not liable to become disconnected. Ensure that the gas pressure is
set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure
may result in leakage of CO gas. Elevated level of CO gas can be hazardous to health and may
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lead to asphyxiation and risk of death. If restricted ventilation is suspected, then other methods of
ensuring a safe environment must be considered. These may include atmosphere monitoring
and warning devices. Keep proper gas pressure to avoid gas leak.
The optional kit MCO-5GC is available for this incubator. MCO-5GC is an automatic gas supply
changeover kit which switches the gas supply when one gas cylinder becomes empty.
The connection of CO gas cylinder is different between with MCO-5GC and without MCO-5GC.
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Use a liquefied CO gas cylinder, not a siphon (dip tube) type. The CO gas should be 99.5% or purer.
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1. Install a pressure regulator (optional accessory MCO-100L) on the cylinder. Or use a regulator rated
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at 25MPaG (250kgf/cm G, 3600psiG) on the primary side and 0.2MPaG (2.0kgf/cm G, 30psiG) on the
secondary side.
2. The connecting port for CO gas is located at the rear left side of the incubator (see page 11). Use
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the port A only when MCO-5GC is not installed. With MCO-5GC installed, use the port A and B.
< When MCO-5GC is not installed >
Using the gas supply pipe provided, connect the pressure regulator to the CO connecting port A located
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at the rear left hand side of the incubator.
< When MCO-5GC is installed >
Two gas cylinders having CO gas regulator are necessary. Connect the main cylinder to the CO
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connecting port A and connect the sub-cylinder to the port B.
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3. Set the CO pressure on the secondary side to 0.03MPaG (0.3kgf/cm G, 4.3psiG) (at gas injection).
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Excessive pressure may cause disconnection of internal pipes inside the incubator which will result in
leakage of CO gas into the atmosphere. Elevated level of CO gas can be hazardous to health and
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may lead to asphyxiation and risk of death. The repair of the incubator will be necessary if the
internal pipe is disconnected.
4. Check that no gas is leaking at any point where the pipe connects with the CO regulator or the
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incubator.
Note:
• Refer to "Procedure for replacement of gas cylinder" enclosed with the unit at the time of replacement.
• The incubator, including the gas supply pipes and services must be examined at frequent intervals to
ensure they are safe. Ensure that items such as pipes are replaced if there is any sign of deterioration.
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