Zeiss Axiolab 5 Instruction Manual

Upright microscope for routine and entry-level research

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Instruction Manual

ZEISS Axiolab 5, Axiolab 5 materials

Upright Microscope for Routine and Entry-Level Research

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Page 1 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials Upright Microscope for Routine and Entry-Level Research...
Page 2 Effective from: 05/2024 © 2024 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor reproduced or transmitted in any form or by any means - including electronic or mechanic methods, by photocopying, recording or by any in- formation or filing system.
Page 3: Table Of Contents
Binocular Tube ..................34 3.2.9 Eyepieces ....................38 3.2.10 Eyepieces with Eyepiece Reticles............38 3.2.11 Nosepiece with Objectives..............39 3.2.12 Condenser Carrier ................. 39 3.2.13 Condensers ................... 40 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 4 Setting Up for Reflected Light Polarization Microscopy ......87 5.5.4 Setting Up for Reflected Light Fluorescence Microscopy ......89 Switching Off the Microscope ................92 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 5 10.5.7 Color Filter Carrier 3x for Filter d=32mm ..........127 10.5.8 Assembling Polarizer or Color Filter Carrier onto the Condenser Carrier . 128 10.6 DIC Slider C 6x20 ....................129 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 6 10.12.1 Preparing the Binocular Tubes ............... 150 10.12.2 Assembling the Dual Observation ............152 10.12.3 Operating the Dual Observation ............153 Revision History................154 Glossary..................155 Index ....................156 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 7: About This Instruction Manual
1 About this Instruction Manual | 1.1 Text Conventions and Link Types 1 About this Instruction Manual This Instruction Manual (further called "document") is considered to be part of the Axiolab 5, Axi- olab 5 materials, herein after referred to as "microscope".
Page 8: Explanation Of Warning Messages And Additional Information
NOTICE indicates a potentially harmful situation which, if not avoided, may result in property damage. Info Provides additional information or explanations to help the operator better understand the contents of this document. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 9: Explanation Of Symbols
In vitro diagnostic medical device Serial number Catalogue number WEEE label: Do not discard as unsorted waste. Send to separate collection facilities for recovery and recycling Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 10: Further Applicable Documents
Axiocam 202 mono § Axiocam 208 color § Multidiscussion Units 1.5 Contact If you have any questions or problems, contact your local ZEISS Sales & Service Partner or one of the following addresses: Headquarters Phone: +49 1803 33 63 34 Fax:...
Page 11: Safety
Carl Zeiss Suzhou Co., Ltd., Suzhou, China 2.1 Intended Purpose The Axiolab 5 microscopes are instruments for general microscopic imaging for the in vitro exami- nation of various biological samples including samples collected from humans or animals. This imaging provides information to further assess physiological and pathological conditions.
Page 12: General Safety Information
The training will be documented by ZEISS and its completion is to be confirmed by the operator. Special application training is offered for a fee. Current training dates, additional information and the registration form can be found at www.zeiss.com...
Page 13: Order And Use Of Spare Parts
ZEISS 2 Safety | 2.2 General Safety Information 2.2.3 Order and Use of Spare Parts Using spare parts that are not provided by ZEISS can be hazardous or can lead to property dam- age. § Unless authorized by ZEISS, all spare parts should be installed by a ZEISS service representa- tive.
Page 14: Optical Risk Grouping
Only use the handle, if applicable, for transport of the microscope. Otherwise hold the micro- scope with one hand and the base plate with the other hand. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 15: Electrical Hazards
The protective earth connection must not be impaired by the use of extension cables. § Always use the power cords supplied by ZEISS. When an unsuitable power cord is used, ZEISS can no longer guarantee the electrical safety and functionality of the microscope. §...
Page 16: Hazards Generated By Materials And Substances
Hazards eye injuries. Consumables that are not approved by ZEISS can lead to property damage. Consult your ZEISS Sales & Service Partner to learn what consumables you can order and how to handle them. Immersion Oil Ensure that no immersion oil enters the surface water or the sewage system. The immersion oil can cause skin irritation.
Page 17: Hazards Generated By Radiation
Check all warning labels for availability and legibility. § Immediately replace damaged or illegible warning labels. In case a label is missing, contact your ZEISS service representative for free of charge replacement. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 18: Labels On The Axiolab 5
May be harmful UV Radiation to the eyes. Do not stare at operating lamp. May be harmful to the eyes. UDI label Microscope type label Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 19: Safety Devices And Interlocks
To verify the safety of the microscope and its components, contact your ZEISS service representa- tive and keep the service logs and logbooks. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 20: Product And Functional Description
ZEISS 3 Product and Functional Description The Axiolab 5, Axiolab 5 materials is a transmitted-light microscope of compact design with a small footprint. The microscope provides high resolution, infinity-corrected objectives for transmit- ted-light techniques and for reflected-light techniques, depending on the microscope type.
Page 21: Main Components
3.1.1 Axiolab 5 Bio-TL Fig. 2: Main components - Axiolab 5 Bio-TL Eyepieces [ 38] Binocular tube [ 34] Stand Bio/TL Mechanical stage [ 42] Condenser [ 40] Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 22: Axiolab 5 Bio-Tl/Fl
3.1.3 Axiolab 5 Pol-TL Fig. 4: Main components - Axiolab 5 Pol-TL Eyepieces [ 38] Binocular tube [ 34] Stand Pol-TL Rotary stage [ 42] Condenser [ 40] Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 23: Axiolab 5 Pol-Tl/Conoscopy
3.1.5 Axiolab 5 Pol-TL/RL Fig. 6: Main components - Axiolab 5 Pol-TL/RL Eyepieces [ 38] Binocular tube [ 34] Stand Pol-TL/RL Rotary stage [ 42] Condenser [ 40] Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 24: Axiolab 5 Mat-Tl/Rl
3.1.6 Axiolab 5 Mat-TL/RL Fig. 7: Main component - Axiolab 5 Mat-TL/RL Eyepieces [ 38] Binocular tube [ 34] Stand Mat-TL/RL Mechanical stage [ 42] Condenser [ 40] Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 25: Controls And Functional Elements On Components
Focusing drive – coarse adjustment (left side) Focusing drive – fine adjustment (left Power switch On/Off side) Slot for 6x20mm slider Nosepiece [ 39] with 5 positions BF Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 26: Axiolab 5 Bio-Tl/Fl Stand
Stage carrier for mechanical stages Snap button (left side) Focusing drive – coarse adjustment (left side) Focusing drive – fine adjustment (left Power switch On/Off side) 4-position reflector turret Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 27: Axiolab 5 Pol-Tl Stand
Focusing drive – coarse adjustment (left side) Focusing drive – fine adjustment (left Power switch On/Off side) Nosepiece with 5 positions BF Slot for 6x20mm slider Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 28: Axiolab 5 Pol-Tl/Conoscopy Stand
(right side) Snap button (right side) Slot for 6x20mm slider Luminous-field diaphragm Stage carrier for rotary stages (also suit- able for mechanical stages) Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 29: Axiolab 5 Pol-Tl/Rl Stand
4x centerable, 1x fixed) 3.2.5 Axiolab 5 Pol-TL/RL Stand This section shows the functional elements and controls of stand Pol-TL/RL. Fig. 12: Functional elements and controls - stand Pol-TL/RL Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 30 Nosepiece, with 5 positions BF DF Pol Slot for polarizer slider 6x30mm, re- Slot for 2-position filter slider, reflected flected light (slider inserted) light (covered) Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 31: Axiolab 5 Mat-Tl/Rl Stand
Focusing drive – fine adjustment (right side, finger wheel) Focusing drive – coarse adjustment Snap button (right side) (right side) Slot for 6x20mm slider Luminous-field diaphragm Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 32: Functions Of Stand Keys And Display Elements
The indicator light starts blinking in RED after 3 s until 20 s is reached. After 20 s, the indicator light turns to GREEN con- tinuously. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 33 * Short press means: hold less than 1 second, then release. ** Long press means: hold at least 1.5 seconds. *** Blinking: the indicator light alternately goes on/off at 500 ms intervals Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 34: Binocular Tube
30° § field of view 23 mm Fig. 14: Binocular Photo Tube 30°/23 (50:50) Eyepiece socket Binocular section Angle scale Camera port (covered) Dovetail ring mount Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 35 Shift knob to front (eye symbol): 100% light to eyepieces § Shift knob to the rear (camera sym- bol): 100% light to camera Dovetail ring mount Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 36 The following features and controls are available: § Reverse image § Camera port with splitting ratio 50:50 § Viewing angle -2° to 28° § Field of view 23 mm Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 37 Field of view 25 mm Fig. 18: Binocular ergo photo tube -2° to 28°/25 (50:50), reversed image Binocular section Eyepiece socket Angle scale Camera port (covered) Dovetail ring mount Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 38: Eyepieces
Red dot, corresponds to the zero White dot, corresponds to the zero diopter setting when a reticle is inserted diopter setting when no reticle is in- serted Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 39: Nosepiece With Objectives
Fig. 22: Condenser carrier Knurled knob for vertical adjustment Centering hex screw (left/right), op- (left/right) tional: knurled screw Orientation groove Main spring Fastening screw for height stop Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 40: Condensers
Display field of the adjusted modulator Knurled ring for adjusting the position disk position of the modulator disk Modulator disk with 5 positions for condenser modules Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 41 § Compatible with low-power system for objectives 2.5x/4x Fig. 25: Condenser 0.8 BF WD=5.8mm Lever for setting the aperture di- Front lens aphragm Dovetail ring mount Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 42: Stages
X Coaxial knurled knob for Y adjustment Coaxial knurled knob for X adjustment Vernier scale for display of the adjust- ment range in Y Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 43: Reflector Turret
Field for the supplied stickers, the stick- ers can be labeled with the filter combi- nation data of the reflector module and pasted on the corresponding field Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 44: Storage Compartment
Users can manually turn it off, so the field of view remains the brightness of the first objective or turret until the second one arrives in the position. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 45: Microscopy And Contrast Techniques
(constructive and destructive interference) – de- pending on their phase. As a result, these interferences create image contents with intensity dif- ferences visible to the human eye. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 46: Transmitted Light Polarization
The gray-white color appearing first in the bright position in the above example corresponds to a path difference of 150 nm according to the Michel-Lévy color chart). Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 47 (red: 550 nm). In this case, the interference color of the sample visibly changes from gray-white to orange (resulting path difference = 400 nm). Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 48 With optical anisotropy all transparent samples show the interference colors that are characteristic to them. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 49 → blue → yellow) Quartz wedge Arrow = (direction of Direction of motion at movement insertion) Lambda/4 plate (position of black spots) Tab. 1: Determining optical character Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 50: Transmitted Light Polarization For Coniscopic Observation
The objective collects the light reflected on the sample and creates, with the tube lens, the microscopic intermediate image. This image can then be examined visually or documented using a camera. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 51: Reflected Light Darkfield Microscopy Using The Köhler Method
The spectra of the excitation and the emission filter must match very closely. They must be inserted in a Reflector Module FL EC P&C reflector module together with the according Dichroic Beam splitter. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 52: Installation
It is recommended to keep the original packing and store it away for later use, e.g. for stowing the microscope during periods of non-use or for returning the microscope to the manufacturer for repair. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 53: Assembling The Binocular Tube
5. Rotate the binocular tube into the desired observation position. 6. Re-tighten the clamping screw with the hex key. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 54: Assembling The Binocular Tube With An Intermediate Plate
7. Rotate the binocular tube into the desired observation position. 8. Re-tighten the clamping screw of the intermediate plate with the hex key. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 55: Assembling Components Into The Binocular Tube
3. Instead of an eyepiece insert an auxiliary microscope or pinhole diaphragm one eyepiece socket. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 56: Assembling The Objectives
7. Apply the protective cap to prevent sample marker from drying out. 8. Always replace the dust protection caps on any empty positions on the nosepiece. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 57: Assembling Condensers
6. Screw in the centering screws until they engage with the dovetail ring of the condenser. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 58: Assembling The Dry Darkfield Condenser
Z sits horizontally on the condenser carrier. 7. Screw in the centering screws until they engage with the dovetail ring of the condenser holder Z. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 59: Assembling The Condenser, Chromatic-Aplanatic 0.9 Bf Df Phc Dic
7. Press the condenser with the dovetail ring against the mainspring of the condenser carrier until the condenser sits horizontally on the condenser carrier. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 60: Assembling The Modulator Disk In The Condenser 0.9 Bf Pol
The guide serves as a stop for the modulator disk. The pin of the disk’s clamping screw must slide into the orientation groove of the condenser. 4. Tighten the disk’s clamping screw. 5. Replace the condenser in its carrier. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 61: Loading The Reflector Turret
7. Apply the supplied stickers with the filter combination data of the respective module to the corresponding field of the cover cap (positions 1 to 4). Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 62: Removing Reflector Modules
The microscope is switched off. ü Procedure 1. Connect the power cord to the mains socket of the stand. 2. Connect the power cord to the mains. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 63: Operation
Before installing or removing the light source always make sure it is switched off. Prerequisite ü The microscope is connected to the mains. Procedure 1. Switch the microscope on using the power switch On/Off. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 64: Adjusting
Both microscopic images including the eyepiece reticle are thus in focus. From this point, use only the focusing drive for any subsequent focusing activity. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 65: Adjusting The Height Stop On The Condenser Carrier
2. Focus the sample with the focusing drive. 3. Close the luminous-field diaphragm. 4. Adjust the condenser vertically until a sharp im- age is visible. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 66: Using The Light Manager Function
GREEN / GREEN / GREEN à The indicator light blinks in the following sequence, when the Light Manager is switched off: GREEN / ORANGE / GREEN Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 67 à Dazzle protection function disabled: The indicator light blinks ORANGE twice. à Dazzle protection function enabled: The indicator light blinks GREEN twice. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 68: Setting The Eco/Permanent Mode
2. Adjust the image brightness using the Intensity/LM knob on the microscope stand. 3. Insert the high-contrast sample into the sample holder of the stage. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 69 6. Set the condenser with the knurled knob for vertical adjustment to the upper stop. 7. Swivel in the 10x objective on the nosepiece. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 70 11. Center the luminous-field diaphragm using the two centering screws on the condenser carrier Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 71: Setting Up For Transmitted Light Darkfield Microscopy
ü A suitable condenser for transmitted light darkfield microscopy is installed [ 57]. ü The illumination is adjusted for transmitted light brightfield microscopy [ 68]. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 72 3. Swivel the objective with the highest possible aperture into position on the nosepiece. 4. Place the sample on the stage. 5. Focus the sample. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 73 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 74: Setting Up For Transmitted Light Phase Contrast Microscopy
The microscope is adjusted for transmitted light brightfield microscopy [ 68]. § The rotary stage is centered [ 121]. § The objectives are centered [ 122]. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 75 Optically anisotropic substances may remain dark when an isotropic direction, e.g. from optically single-axle or double-axle crystals, is oriented parallel to the observation direction. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 76 6. Insert the determined compensator into the slot as far as it will go. 7. Use the enclosed operating instructions for measurement preparation and measurement procedure. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 77 If the crystal needles oriented parallel to the gamma direction of the lambda plate are yellow, and the crystal needles lying at a right angle to the gamma direction are blue, the crystals are monosodium urate crystals (gout). Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 78 ü The illumination is adjusted for transmitted light brightfield microscopy [ 68] using the KÖH- LER method. Lambda compensator or lambda/4 compensator is available. ü Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 79 5. Place the Pol adjustment sample on the microscope. 6. Turn the rotary stage until the adjustment sample appears dark. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 80 6. Insert the determined compensator into the slot as far as it will go. 7. Use the enclosed operating instructions for measurement preparation and measurement procedure. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 81 The axial figure will appear in the field of view. 9. Bring the axial figure into focus using the setting wheel 10. Determine the optical character of the crystal. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 82 10. For the detection of gout or pseudo-gout, select crystal needles that are oriented in the gamma direction (see marking on the lambda plate). Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 83: Setting Up For Reflected Light Techniques
Intensity/LM knob on the microscope stand. 3. Place a high-contrast reflected light sample into the sample holder of the mechanical stage. 4. Swivel the 10x objective into the beam path. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 84 A to a medium position. 8. Adjust the knurled wheel of the luminous-field diaphragm F so that the field diaphragm be- comes visible in the field of view. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 85 The illumination is now adjusted according to the KÖHLER method. Info Never use the aperture diaphragm for controlling image brightness. Use the Intensity/LM knob for illumination intensity! Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 86: Setting Up For Reflected Light Darkfield Microscopy
4. Completely open the aperture diaphragm A. 5. Switch off or remove the neutral filter as necessary. 6. Place the sample on the stage. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 87: Setting Up For Reflected Light Polarization Microscopy
1. If required, push the RL button for reflected light illumination. 2. Swivel in the P&C (for DIC or Pol) reflector module on the reflector turret. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 88 6. Focus the sample. 7. Observe the sample in the polarization contrast now present while turning the Pol rotary stage. The illumination is now adjusted for polarization microscopy. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 89: Setting Up For Reflected Light Fluorescence Microscopy
The microscope is adjusted for reflected light brightfield microscopy [ 83]. Procedure 1. Slide the fluorescence protection shield into the compensator slit above the nosepiece. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 90 Ph objective). 5. Seek the sample detail to be examined. 6. Adjust the light intensity by turning the Inten- sity/LM knob. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 91 11. Adjust the light intensity for reflected light by turning the Intensity/LM knob. 12. Focus the sample. The illumination is now adjusted for fluorescence microscopy. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 92: Switching Off The Microscope
ZEISS 5.6 Switching Off the Microscope Procedure 1. Switch the microscope off using the power switch On/Off. 2. Cover the microscope with the dust cover. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 93: Care And Maintenance
Make sure that no cleaning liquid or moisture gets inside the microscope and its compo- nents. In case of damage, the affected parts of the microscope must be taken out of operation. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 94: Maintenance Schedule
2. Wipe optical surfaces in a circular motion to- wards the edge of the optics with slight pres- sure. 3. Dry with a lint-free cloth. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 95: Removing Water-Soluble Contamination
Switch off the microscope and its components prior to opening or cleaning. Disconnect live parts from the power supply. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 96: Mechanical Stages
6. Bring the microscope back into operation. 6.3.4 Mechanical Stages Axiolab 5 stands are fitted with the respective mechanical stage at the factory according to cus- tomer requirements. The friction adjustment of the coaxial knurled knobs is set at an average value at the factory.
Page 97 Large friction adjustment: (counter-clockwise) It should not be shifted more than one revolution. 4. Re-insert the adjusting pin into the coaxial knurled knob for the Y adjustment. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 98: Troubleshooting
7 Troubleshooting | ZEISS 7 Troubleshooting The following table provides hints for solving common problems. If you cannot solve the problem or if you are unsure about a certain technical difficulty, contact your local ZEISS service represen- tative. Symptom Cause...
Page 99 The image brightness is unac- Adjust the lamp brightness or ceptable. insert a conversion filter. The binocular tube is optically/ Call in service personnel for mechanically misaligned. check/repair. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 100 A wrong or missing reflector Check the reflector turret and module for reflected light is in make sure the correct reflector use. is in use. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 101: Resetting The Microscope To Factory Settings
It blinks GREEN after 20 s are reached. When the indicator light stops blinking and remains GREEN, the reset to the default factory setting is successful. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 102: Decommissioning And Disposal
Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 103: Disposal
In general, a well-visible warning sign must be affixed to the article itself and to the outside of the packaging, together with detailed information on the type of contamination. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 104: Technical Data And Conformity
The system must be connected to a building earth point at all times. Fuses in the stand 2x T 3.15 A/H, 5x20 mm (in compliance with IEC 127) Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 105 410/509 telescopic, height, upright image scopic Binocular tube 20°/23 20° - None - 442/481 Binocular photo tube 20°/23 Pol 20° - None - 442/481 (100:100) Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 106: Applicable Standards And Regulations
Member States relating to the making avail- able on the market of electrical equipment designed for use within certain voltage limits Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 107 Electrical equipment for measurement, control and labora- tory use - EMC requirements - Part 2-6: Particular require- ments - In vitro diagnostic (IVD) medical equipment Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 108: Accessories And System Expansions
Only the following accessories may be used with the microscope as their safe use has been confirmed by ZEISS. Only original parts from ZEISS may be used. Check in advance whether your microscope can be retrofitted with a system expansion or accessories.
Page 109 LED module 505 nm for FL § LED module 470 nm for FL § LED module 385 nm for FL § LED module 565 nm for FL § Illuminator TL/RL LED 10 Axiolab Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 110: Assembling The Reversible Eyecups
Sometimes the eyeglass protection rings are seated very tightly in the eyepiece groove, so you may need a blunt sample (wooden stick) to prod them off. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 111: Binocular Tube
The following features and controls are available: § upright image § camera port with toggle graduation (100:0/0:100) § viewing angle 20° § field of view 23 mm Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 112: Binocular Ergo Photo Tube 20°/23 (100:0/0:100)
20° § field of view 23 mm, usable 22 mm § vertical adjustment of 44 mm with vertical scale Fig. 35: Binocular Ergo Photo Tube 20°/23 (100:0/0:100) Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 113: Binocular Photo Tube 30°/23 (98:2), Reversed Image
Viewing angle 30° § Field of view 23 mm Fig. 36: Binocular photo tube 30°/23 (98:2), reversed image Eyepiece socket Binocular section Angle scale Camera port Dovetail ring mount Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 114: Binocular Photo Tube 30°/23 (100:0/30:70/0:100), Reversed Image
(as in a mechanical stage) to allow easy positioning of the object in the x- and y-axis, and movement along, and rotation about the z-axis. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 115: Assembling A Mechanical Stage And Sample Holder
3. Turn the stage to orient it in an XY direction and tighten the fastening screws. 4. Place the sample holder on the stage and fasten the two clamping screws . 10.3.2 Removing a Mechanical Stage and Sample Holder Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 116: Rackless Mechanical Stage, 75X30 R
X Coaxial knurled knob for Y adjustment Coaxial knurled knob for X adjustment Vernier scale for display of the adjustment range in Y Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 117: Mechanical Stage, 75X30 R
X Rack Coaxial knurled knob for Y adjustment Coaxial knurled knob for X adjustment Vernier scale for display of the adjustment range in Y Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 118: Rotary Stage Pol 360° With Sample Guide
Spring lever Click stop every 45° Vernier scale for display of the adjust- Control knob to enable/disable the clip ment range in Y stop function Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 119 4. If available, loosen the two clamping screws on the sample guide 5. Remove the sample guide by lifting it upwards. 6. If available, remove the clamping device from the rotary stage. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 120 7. If required, insert the sample guide with the two cylindrical pins on the underside into the holes provided and tighten the two clamping screws . Use a 2 mm hex key. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 121 5. Check whether the sample detail moves when the stage is rotated again; repeat the proce- dure, if required. 6. Re-tighten the screw cap. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 122 6. Check whether the sample detail moves when the stage is rotated again; repeat the proce- dure if required. 7. Center the other three objectives in the same manner. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 123: Analyzer Sliders
Fig. 43: Analyzer slider TL/RL with lambda plate, each rotatable +/- 10° Empty position Analyzer and lambda plate Handle for adjusting the lambda plate Handle for adjusting the analyzer Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 124: Polarizers
The polarizer is mounted on the bottom of the condenser carrier. Fig. 45: Polarizer D, 90°, rotatable, removable Locking pin Lever for rotating the polarizer Handle of the polarizer for swivelling in/ Holding pin Locking clamp Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 125: Polarizer, Fixed, With Lambda Plate, Rotatable
Fig. 47: Polarizer, rotatable, with color filter carrier Locking pin Handle of the polarizer for swivelling in/ Handle of the color filter carrier Holding pin Locking clamp Polarizer Color filter carrier Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 126: Circular Polarizer D
Fig. 49: Low-power system for objectives 2.5x/4x Centering screw (2x) Locking pin Handle of the low-power system for Holding pin swivelling in/out Locking clamp Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 127: Color Filter Carrier 3X For Filter D=32Mm
The color filter carrier is mounted on the bottom of the condenser carrier. The three filter carriers can be swivelled in/out separately. Fig. 50: Color filter carrier 3x for filter d=32mm Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 128: Assembling Polarizer Or Color Filter Carrier Onto The Condenser Carrier
Proceed in the reverse order for removal. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 129: Dic Slider C 6X20
Knurled ring for adjusting the position Sliding control for setting the aperture of the 5-positions turret disk diaphragm Dovetail ring mount Display field of the adjusted turret disk position Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 130: Assembling The Condenser, Achromatic-Aplanatic 0.9 Bf Df Phc Dic
7. Screw in the centering screws until they engage with the dovetail ring of the condenser. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 131: Centering The Darkfield Diaphragm Of The Condenser
4. Check the centering and the overlap of the lighter annular phase diaphragm (in the con- denser) with the darker phase ring (in the ob- jective). Both rings must be centered and overlapping Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 132: Light Source For Transmitted Light
2. Swing the cover down. 3. Remove it from the retaining channels on the stand. 4. Set the cover aside. Proceed in the reverse order for installing. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 133: Exchanging The Halogen Lamp
9. Insert the cable of the lamp plug into the stand so that it is not damaged when the cover is attached. 10. Insert the cover [ 132]. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 134: Exchanging The Led Light Source
3. Pull the old LED light source with adapter out of the holding tube. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 135 - Release the pressure until the clamping bracket opens and engages on both sides in the retaining elements. 5. Insert the cover [ 132]. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 136: Light Source For Reflected Light
2. Swing the cover slightly upwards and apply pressure from below to remove it from the stand. Proceed in the reverse order for installing. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 137: Exchanging The Halogen Lamp
LED light source in the adapter [ 139] § assembling the LED light source including adapter [ 139] § assembling the cover [ 136] Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 138 3. Pull the old LED light source with adapter out of the holding tube Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 139 - Release the pressure until the clamping bracket opens and engages on both sides in the retaining elements. 5. Insert the cover [ 136]. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 140: Exchanging The Led Modules Of The Fluorescence Light Source
Avoid exposure of skin to the radiation. Use suitable protective equipment/protective clothing if required. Before installing or removing the light source always make sure it is switched off. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 141 10.9.4.3 Assembling the Module Holder Procedure 1. Screw the module holder into the stand socket to fix the modules in place. 2. Insert the cover [ 136]. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 142: Axiocam 202 Mono/208 Color
10.10.1 Assembling the Axiocam 202 mono or Axiocam 208 color C-mount camera adapter Parts and Tools  USB (Commercial Micro-D) cable (USB 2.0)  HDMI cable  Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 143: Operating Modes Using The Axiocam 202 Mono/208 Color
Record and save images on the USB drive § Record and save videos on the USB drive Fig. 54: Axiocam as standalone system USB Type-C drive (included in package) Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 144 Fig. 55: Axiocam connected to an HD monitor, TV or projector USB hub (input type C to output type A) USB Type-C drive provided in package Mouse, keyboard Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 145 Advanced features in Labscope/Matscope Fig. 56: Axiocam connected with Labscope/Matscope via a Wi-Fi dongle USB Wi-Fi dongle (please see the ZEISS website for the recommended model) Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 146 Record and save images via software § One-key fluorescence (this works only with Axiolab Bio-TL/FL) § Advanced features in Labscope/Matscope Fig. 57: Axiocam connected with Labscope/Matscope via a WLAN router Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 147 Record and save images via software § One-key fluorescence (this works only with Axiolab Bio-TL/FL) § Advanced features in Labscope/Matscope Fig. 58: Axiocam connected with Labscope/Matscope via a USB Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 148: Assembling The Filter 32X4 Mm On The Luminous-Field Diaphragm Operating Ring
3. To secure the filter, insert the filter clamp on the luminous-field diaphragm operating ring Proceed in the reverse order for removal. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 149: Dual Observation
Joystick for LED arrow position control server Observation tube mount for co-ob- DC input socket (5V) server ON/OFF switch Filter plate for LED arrow color chang- Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 150: Preparing The Binocular Tubes
2. Remove the tube lens using the ring tool 3. Store the tube lens in dust-free conditions. 4. Turn the binocular tube. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 151 5. Insert the required glass filter into the adaptation set. 6. Tighten the retaining ring using the mounting tool 7. Screw the adaptation set into the binocular tube. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 152: Assembling The Dual Observation
4. Install the binocular tube for the co-observer 5. Install the eyepieces for the co-observer. 6. Connect the plug-in power cable to the microscope stand. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 153: Operating The Dual Observation
6. Switch off the dual observation by turning the rotary button counterclockwise. à The blue LED below the knob goes out. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 154: Revision History
04/2023 § Editorial rework § Implementation of UKCA marking § 05/2022 Implementation of revision history § Adaptation to Regulation (EU) 2017/746 (IVDR) Tab. 2: Revision History Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 155: Glossary
Contrary to traditional polarization interferometers, work in this technique is carried out in circularly po- larized light. Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 156: Index
12 mounting 59 Condenser carrier 39 Darkfield condenser Halogen lamp, replacing 133, 137 installing 58 Coniscopic observation 50 Connecting to the mains 62 Contamination 103 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 157 Mechanical stage 42, 96, 116, 117 adjusting the friction 97 installing 115 removing 116 setting the drive length 96 Modulator disk installing 60 Nosepiece 39 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 158 Tool 44 Training 12 Transmitted light polarization 50 Transmitted light brightfield 45, 68 darkfield 45, 72 phase contrast 45, 74 polarization 46, 74 Transport 102 Instruction Manual ZEISS Axiolab 5, Axiolab 5 materials | en-US | Rev. 11 | 430037-7444-001...
Page 160 Carl Zeiss Microscopy GmbH Carl-Zeiss-Promenade 10 phone: +49 1803 33 63 34 07745 Jena fax: +49 3641 64 3439 Germany info.microscopy.de@zeiss.com www.zeiss.com/microscopy...

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