Acquiring The Image - BIO RAD VERSADOC User Manual

4.3.9

Acquiring the Image

To collect the sample image, simply press the ACQUIRE button. The yellow LED on the
front panel of the VersaDoc will flash during acquisition to indicate that the image is being
captured. The software will automatically set all instrument collection parameters and
prompt you to take additional steps for best results.
NOTE: You will notice that after the initial image capture, the software also acquires a dark image. This
image is taken for the same amount of time as the exposure, however, this dark image is acquired with the
camera shutter closed. This provides a very accurate way to measure and remove the dark current noise
from the image.
Once the image is captured, it will be displayed in its own window. You may save the
captured image for storage and/or future analysis.
NOTE: When an application is selected that utilizes UV transilluminator, the software will first acquire an
initial image and then prompt you to remove the sample. You must make sure that the sample platen is free
of any solution from the sample. You will then be prompted to place the VersaDoc Fluorescent Reference
plate.
When your application requires white light transillumination, the software will also prompt
you to place the VersaDoc white light conversion screen on the UV transilluminator platen.
This white light conversion screen absorbs the UV from the UV transilluminator and
converts this broadband UV into white light. See figure 4.5.
Figure 4.5 White Light Conversion Screen
In this application, the software will first take an initial image and then prompt you to
remove the sample.
NOTE: Please make sure that the surface of the VersaDoc white light conversion screen is clean after the
sample has been removed.
After the image has been acquired, its appearance can be optimized. The image may also
be analyzed in various ways using the Quantity One program. Analysis options include
object volume analysis, lane profile analysis including regression analysis and molecular
weight determination, colony counting, fingerprinting, VNTR and differential display studies.
The image and various data reports may also be printed or exported to other software
programs. Please refer to the Quantity One software manual for detailed instructions.
Note: Please refer to the Quantity One software manual for information concerning additional features
available in the acquisition window for VersaDoc.
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